SRR936194_2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR936194_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	46601
Sequences flagged as poor quality	0
Sequence length	125
%GC	27

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2051	4.401193107444047	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1742	3.738117207785241	No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1429	3.0664578013347352	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	892	1.914122014549044	No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	792	1.69953434475655	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	503	1.0793759790562434	No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	438	0.9398939936911226	No Hit
ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	367	0.787536748138452	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	280	0.6008454754189824	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	204	0.4377588463766872	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	187	0.4012789425119632	No Hit
AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	168	0.3605072852513895	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	164	0.3519237784596897	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	162	0.34763202506383983	No Hit
ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	152	0.32617325808459047	No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	137	0.2939851076157164	No Hit
AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	114	0.24462994356344286	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	111	0.23819231346966802	No Hit
AAAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	102	0.2188794231883436	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	102	0.2188794231883436	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	97	0.20815003969871892	No Hit
AAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	85	0.18239951932361967	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.17596188922984485	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81	0.17381601253191992	No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC	81	0.17381601253191992	No Hit
TAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	77	0.16523250574022016	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	65	0.13948198536512094	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	65	0.13948198536512094	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	63	0.13519023196927105	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	62	0.13304435527134612	No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61	0.13089847857342116	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	59	0.1266067251775713	No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	58	0.12446084847964635	No Hit
GAACAAAAAAACACAAAAAACATGGGGGGGAGGTAGTGACGAAAAAAAAA	56	0.12016909508379649	No Hit
AAAAAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53	0.11373146499002167	No Hit
GAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52	0.11158558829209672	No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT	52	0.11158558829209672	No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA	51	0.1094397115941718	No Hit
GAACAAAAAAACACAAAGAACATGGGGGGGAGGTAGTGACGAAAAAAAAA	47	0.10085620480247204	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACAAGG	40	1.546141E-10	104.11843	2
GTACATA	50	9.094947E-12	95.193985	1
TACAAGA	25	1.7088547E-5	95.193985	2
TACATGA	40	1.8613719E-8	89.24437	2
TACATAA	50	9.113137E-10	83.29474	2
GGAAACA	120	0.0	79.32832	1
CGCAGAA	30	4.222692E-5	79.32832	2
GAACAAA	520	0.0	74.37031	1
GGAATCA	50	8.747156E-8	71.39549	1
GTACAAA	125	0.0	71.39549	1
CATAGGA	25	0.0020449592	71.39549	6
ACATAGG	35	9.063628E-5	67.995705	5
GAAAACA	405	0.0	63.16885	1
GAGAACA	370	0.0	61.10425	1
AGTGCCA	15	0.0042254203	59.496246	104-105
TCTGCTG	15	0.0042254203	59.496246	66-67
AGAACAA	250	0.0	59.496243	2
GAAACAA	220	0.0	56.79187	2
ACAACAC	305	0.0	56.5702	5
GAATCAA	65	5.369984E-7	54.919613	2

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content