##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936194_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 46601 Sequences flagged as poor quality 0 Sequence length 125 %GC 27 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 17.559515890216947 15.0 7.0 31.0 2.0 33.0 2 19.127851333662367 23.0 7.0 31.0 2.0 34.0 3 20.372331065856955 24.0 7.0 31.0 2.0 34.0 4 21.0028969335422 22.0 7.0 35.0 2.0 37.0 5 25.49219973820304 31.0 17.0 35.0 2.0 37.0 6 21.89277054140469 26.0 8.0 35.0 2.0 37.0 7 22.95424990880024 30.0 8.0 35.0 2.0 37.0 8 21.06321752752087 23.0 7.0 35.0 2.0 37.0 9 20.721336452007467 18.0 7.0 37.0 2.0 39.0 10-11 22.286077551983865 26.5 7.0 37.0 2.0 39.0 12-13 22.710639256668312 28.5 7.0 37.0 2.0 39.0 14-15 23.337020664792604 29.0 7.0 38.0 2.0 41.0 16-17 23.57680092701873 30.0 7.0 38.0 2.0 41.0 18-19 22.995311259415033 30.0 2.0 38.0 2.0 41.0 20-21 22.569290358575998 30.0 2.0 37.0 2.0 41.0 22-23 21.844928220424457 28.5 2.0 36.5 2.0 41.0 24-25 20.968509259457953 27.0 2.0 36.0 2.0 41.0 26-27 20.519806441921844 26.0 2.0 36.0 2.0 40.5 28-29 20.06236990622519 25.0 2.0 36.0 2.0 40.0 30-31 19.578185017488895 25.0 2.0 36.0 2.0 40.0 32-33 19.038754533164525 25.0 2.0 35.0 2.0 40.0 34-35 18.48228578785863 23.0 2.0 35.0 2.0 40.0 36-37 17.82530417802193 10.5 2.0 35.0 2.0 40.0 38-39 17.226926460805565 2.0 2.0 35.0 2.0 40.0 40-41 16.898349820819295 2.0 2.0 35.0 2.0 40.0 42-43 16.524741958327077 2.0 2.0 35.0 2.0 40.0 44-45 16.423563872019912 2.0 2.0 35.0 2.0 40.0 46-47 16.24101414132744 2.0 2.0 35.0 2.0 40.0 48-49 15.999742494796248 2.0 2.0 34.0 2.0 40.0 50-51 15.845421772064977 2.0 2.0 34.0 2.0 40.0 52-53 15.643484045406751 2.0 2.0 34.0 2.0 40.0 54-55 15.503894766206734 2.0 2.0 33.5 2.0 40.0 56-57 15.35965966395571 2.0 2.0 33.0 2.0 40.0 58-59 15.141960472951224 2.0 2.0 33.0 2.0 40.0 60-61 14.931707474088538 2.0 2.0 33.0 2.0 40.0 62-63 14.703622239866096 2.0 2.0 33.0 2.0 39.0 64-65 14.478348104117938 2.0 2.0 32.0 2.0 39.0 66-67 14.351805755241305 2.0 2.0 32.0 2.0 39.0 68-69 14.218160554494538 2.0 2.0 32.0 2.0 39.0 70-71 13.986202012832344 2.0 2.0 31.0 2.0 38.0 72-73 13.773717302203817 2.0 2.0 31.0 2.0 37.5 74-75 13.55659749790777 2.0 2.0 30.5 2.0 37.0 76-77 13.274275230145275 2.0 2.0 30.0 2.0 36.5 78-79 13.117465290444411 2.0 2.0 30.0 2.0 36.0 80-81 12.95800519302161 2.0 2.0 30.0 2.0 35.5 82-83 12.783695628849166 2.0 2.0 30.0 2.0 35.0 84-85 12.673064955687646 2.0 2.0 30.0 2.0 35.0 86-87 12.481470354713418 2.0 2.0 30.0 2.0 35.0 88-89 12.264479303019248 2.0 2.0 29.5 2.0 35.0 90-91 12.08974056350722 2.0 2.0 29.0 2.0 35.0 92-93 11.953069676616382 2.0 2.0 29.0 2.0 35.0 94-95 11.877524087465934 2.0 2.0 29.0 2.0 35.0 96-97 11.778953241346752 2.0 2.0 29.0 2.0 35.0 98-99 11.626488701959186 2.0 2.0 29.0 2.0 35.0 100-101 11.480601274650759 2.0 2.0 28.0 2.0 35.0 102-103 11.065213192849939 2.0 2.0 26.5 2.0 34.0 104-105 11.252494581661338 2.0 2.0 28.0 2.0 34.5 106-107 11.20871869702367 2.0 2.0 28.0 2.0 35.0 108-109 11.170715220703418 2.0 2.0 28.0 2.0 35.0 110-111 11.077627089547434 2.0 2.0 28.0 2.0 35.0 112-113 10.98625565974979 2.0 2.0 27.0 2.0 35.0 114-115 10.807858200467802 2.0 2.0 26.5 2.0 35.0 116-117 10.681348039741636 2.0 2.0 25.5 2.0 35.0 118-119 10.563292633205297 2.0 2.0 25.0 2.0 35.0 120-121 10.40110727237613 2.0 2.0 25.0 2.0 35.0 122-123 10.157067444904616 2.0 2.0 25.0 2.0 35.0 124-125 9.46569816098367 2.0 2.0 21.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.149618319722233 1101 2 0.8599367157706332 1101 3 0.9149697607242899 1101 4 1.2991335113419282 1101 5 2.0934120118484145 1101 6 -0.7082212138355928 1101 7 -1.069474945198536 1101 8 -1.02411983399589 1101 9 0.7833523430819298 1101 10-11 -0.2915821553670348 1101 12-13 -0.33224433318151725 1101 14-15 1.043530720786098 1101 16-17 0.284777111467843 1101 18-19 1.1525422182374925 1101 20-21 0.17024882045630463 1101 22-23 0.48391886004787565 1101 24-25 0.8635093411366874 1101 26-27 1.2164235668732069 1101 28-29 1.0405780466601549 1101 30-31 1.107643596085584 1101 32-33 1.2819357474697206 1101 34-35 1.6272995008515636 1101 36-37 1.0499462195195015 1101 38-39 0.9099672356391011 1101 40-41 0.9338183806359943 1101 42-43 0.8091041273781521 1101 44-45 1.007622620374999 1101 46-47 1.2655988361691506 1101 48-49 1.3707185117197476 1101 50-51 1.1329196814932203 1101 52-53 1.1684955788754419 1101 54-55 0.9510534729096705 1101 56-57 0.9699070780150176 1101 58-59 0.8824665592288845 1101 60-61 0.9182201945777706 1101 62-63 1.007918663176845 1101 64-65 1.545812354381649 1101 66-67 1.2225675619736744 1101 68-69 0.8928183939287262 1101 70-71 0.6145896945236569 1101 72-73 0.5091091752160768 1101 74-75 0.07730998227143004 1101 76-77 0.30300357466388483 1101 78-79 -0.0011635200027768633 1101 80-81 0.16010277942437945 1101 82-83 0.17857331275357424 1101 84-85 0.43302873478467063 1101 86-87 0.5171203935064526 1101 88-89 0.7502058223260306 1101 90-91 0.6079233444137291 1101 92-93 0.46718294394896986 1101 94-95 0.24925964562410918 1101 96-97 0.4447084445646112 1101 98-99 0.11977651365713804 1101 100-101 0.5470887906816184 1101 102-103 0.5593280563570815 1101 104-105 0.7207851777677092 1101 106-107 0.972130063630015 1101 108-109 0.8938936341556047 1101 110-111 0.7862348307748199 1101 112-113 -0.09784704806591193 1101 114-115 0.4118585000353132 1101 116-117 0.828032638705249 1101 118-119 1.1431387096199153 1101 120-121 0.984689712324375 1101 122-123 1.4644050630457013 1101 124-125 1.3278527632329293 1102 1 1.1691054992094116 1102 2 1.0235264593603794 1102 3 -1.3993892136346844 1102 4 0.5268258190342365 1102 5 0.33495047338687556 1102 6 -1.6251442907586693 1102 7 1.3536019778783874 1102 8 0.9333160634400102 1102 9 0.3382241379537234 1102 10-11 -1.3044026681875494 1102 12-13 -0.6389109998481821 1102 14-15 -1.432366715111339 1102 16-17 -0.7477869910962625 1102 18-19 0.9640806797759538 1102 20-21 1.8579411281486138 1102 22-23 2.323662449791467 1102 24-25 1.6335093411366834 1102 26-27 2.20821843866808 1102 28-29 0.9567318928140018 1102 30-31 -0.27517691673492806 1102 32-33 0.06296138849535993 1102 34-35 -0.18577742222535676 1102 36-37 -1.0285153189420377 1102 38-39 -0.7715712258993612 1102 40-41 -0.4600277732101574 1102 42-43 -0.9524343341603085 1102 44-45 -1.2105825078301287 1102 46-47 -1.072606292035978 1102 48-49 -1.3703071293058944 1102 50-51 -1.1388751903016523 1102 52-53 -0.8466326262527648 1102 54-55 -0.8904849886287884 1102 56-57 -0.40496471685677626 1102 58-59 -0.623943697181371 1102 60-61 -0.3958823695247915 1102 62-63 -0.7754146701564864 1102 64-65 -0.6536748251055293 1102 66-67 -0.599740130334018 1102 68-69 -0.5582072470969148 1102 70-71 -0.342589792655831 1102 72-73 -0.24935236324546217 1102 74-75 -0.11756181260036414 1102 76-77 -0.2016118099514994 1102 78-79 0.3206313517920947 1102 80-81 0.21856431788591912 1102 82-83 0.1516502358304983 1102 84-85 -0.2074840857281508 1102 86-87 -0.4439052475191865 1102 88-89 -0.22364033152012297 1102 90-91 -1.417974091483707 1102 92-93 -1.105893979127952 1102 94-95 -1.6471506107861487 1102 96-97 -1.363496683640518 1102 98-99 -1.535095281214657 1102 100-101 -1.355731722138895 1102 102-103 -1.1878514308224055 1102 104-105 -1.078958411975881 1102 106-107 -1.03402378252383 1102 108-109 -1.8014909812290103 1102 110-111 -1.5642779897379988 1102 112-113 -1.5645137147325787 1102 114-115 -2.472244064067251 1102 116-117 -1.9396596689870602 1102 118-119 -1.330451033969828 1102 120-121 -1.656592338957676 1102 122-123 -1.9027744241337867 1102 124-125 -1.8521472367670722 1103 1 2.6607894908934036 1103 2 2.0096664455003648 1103 3 2.8001949859495134 1103 4 2.264178556386973 1103 5 1.5626012010376051 1103 6 1.104931939317563 1103 7 1.0521466764230851 1103 8 2.180024310148255 1103 9 2.3451541448837325 1103 10-11 1.372832259047378 1103 12-13 1.9331610722238857 1103 14-15 2.0710982883536673 1103 16-17 1.710632967323697 1103 18-19 2.0768665425618167 1103 20-21 2.750699270906754 1103 22-23 1.3690539951830125 1103 24-25 2.5817075393348823 1103 26-27 2.9602974407470803 1103 28-29 3.464181650263761 1103 30-31 3.2218778303198192 1103 32-33 3.14040421593819 1103 34-35 3.685497699049762 1103 36-37 3.99643270600599 1103 38-39 4.401678947350813 1103 40-41 4.063187750005362 1103 42-43 4.2230680913421175 1103 44-45 3.895100097852481 1103 46-47 4.0736168541871685 1103 48-49 3.8585563495575848 1103 50-51 3.8117485103220474 1103 52-53 3.953090173470036 1103 54-55 3.676909328765529 1103 56-57 3.6939611320690755 1103 58-59 3.3667007934631172 1103 60-61 3.2920940684516466 1103 62-63 3.143504248762433 1103 64-65 3.205992534561833 1103 66-67 3.5993243187304333 1103 68-69 3.2292147903251216 1103 70-71 3.241436541370506 1103 72-73 2.6382082743151773 1103 74-75 2.8259586309200806 1103 76-77 3.2595801512404616 1103 78-79 3.3745121556728996 1103 80-81 3.367039716361317 1103 82-83 3.154248988429252 1103 84-85 3.1130287347846703 1103 86-87 3.498922195308257 1103 88-89 3.4009265430467526 1103 90-91 3.0517071281975134 1103 92-93 3.096462223228249 1103 94-95 2.9493497357141987 1103 96-97 2.746690426546593 1103 98-99 2.6313981352787597 1103 100-101 2.407088790681618 1103 102-103 2.333111840140864 1103 104-105 2.0873617543442844 1103 106-107 1.665733667233619 1103 108-109 1.9850648053267772 1103 110-111 1.8260546505946404 1103 112-113 1.9625133122944476 1103 114-115 2.1729395811163936 1103 116-117 2.3770416477142593 1103 118-119 2.0730486195298248 1103 120-121 2.2550500726847353 1103 122-123 2.0956663243069613 1103 124-125 1.8122671776473425 1104 1 0.8905192206231334 1104 2 0.7529096887436069 1104 3 1.259654445408973 1104 4 0.6966109888194048 1104 5 0.2517903902267946 1104 6 0.1995265339121559 1104 7 0.4170115412879518 1104 8 0.7881324182563638 1104 9 -0.8980890983595131 1104 10-11 -0.22176233554721847 1104 12-13 -1.0127848737220582 1104 14-15 -0.5775503602949819 1104 16-17 -0.16774541105468188 1104 18-19 0.4147043803996553 1104 20-21 -0.0601115399040566 1104 22-23 -0.10391897778996295 1104 24-25 -0.006130298502956322 1104 26-27 0.47381095426059616 1104 28-29 0.7952627313448417 1104 30-31 0.9989048573468473 1104 32-33 0.20121502674899716 1104 34-35 -0.3212590577069925 1104 36-37 0.2464327060059901 1104 38-39 0.5165438122156765 1104 40-41 0.38075531757293035 1104 42-43 0.5203653886394157 1104 44-45 0.8748298275822073 1104 46-47 1.262806043376358 1104 48-49 0.8855833765846093 1104 50-51 1.3860728346463702 1104 52-53 1.520657741037601 1104 54-55 1.1498823017385007 1104 56-57 1.4304476185555597 1104 58-59 0.9883224150847418 1104 60-61 1.4272292035867835 1104 62-63 1.008369113627296 1104 64-65 1.408695237264535 1104 66-67 0.903378372784486 1104 68-69 0.7021877632980935 1104 70-71 1.1535987035326656 1104 72-73 0.4219920580989598 1104 74-75 0.6840667390281894 1104 76-77 0.8812017728620827 1104 78-79 0.5569445881053312 1104 80-81 0.1643370136586153 1104 82-83 0.36370844788871004 1104 84-85 0.6332990050549405 1104 86-87 0.8908140872001482 1104 88-89 0.3536292457494561 1104 90-91 0.6192746957650801 1104 92-93 0.2518676286336543 1104 94-95 0.3277281140925794 1104 96-97 0.06425799411415944 1104 98-99 0.29356029744092105 1104 100-101 -0.2145328309400032 1104 102-103 -0.5114827544537288 1104 104-105 -0.35858419160165944 1104 106-107 -0.3139960624961109 1104 108-109 -0.548718978457007 1104 110-111 -0.6131345385945473 1104 112-113 -0.28748668770555064 1104 114-115 -0.6176009594241467 1104 116-117 -0.5621475414749302 1104 118-119 -0.9134378669566594 1104 120-121 -0.805760738126077 1104 122-123 -0.8975769189362826 1104 124-125 -0.8904355250553593 1105 1 -2.9236236894101886 1105 2 -2.809104380119777 1105 3 -0.7834777278601877 1105 4 -1.7717340685667473 1105 5 -2.873163330617338 1105 6 -2.0459381088127593 1105 7 -3.946187273965659 1105 8 -2.5554440349091294 1105 9 -2.310528935456883 1105 10-11 -2.1363310138145266 1105 12-13 -1.766764881126722 1105 14-15 -1.3526336627755455 1105 16-17 -2.8381452629613833 1105 18-19 -3.439238603680316 1105 20-21 -4.00226259506881 1105 22-23 -4.8243003180343145 1105 24-25 -4.598225818680664 1105 26-27 -4.0836677573277065 1105 28-29 -4.28554067480103 1105 30-31 -3.9073335728641876 1105 32-33 -3.217333658922975 1105 34-35 -4.165668535678115 1105 36-37 -4.496903095548991 1105 38-39 -3.935603540616608 1105 40-41 -3.413487555437065 1105 42-43 -3.4269689319825787 1105 44-45 -3.6177655074788806 1105 46-47 -3.685268743739524 1105 48-49 -3.605993817047377 1105 50-51 -3.3606876244428534 1105 52-53 -3.2682167498916836 1105 54-55 -2.6869373946702346 1105 56-57 -2.6991340178753926 1105 58-59 -2.3755243083510234 1105 60-61 -2.2484464720888937 1105 62-63 -2.3747297386496378 1105 64-65 -1.9924524858009978 1105 66-67 -1.910857095560571 1105 68-69 -1.9457204188566628 1105 70-71 -1.9296112187183514 1105 72-73 -1.7771008704460254 1105 74-75 -1.8021420725230897 1105 76-77 -1.5864028180301784 1105 78-79 -1.3491087254822283 1105 80-81 -1.2148743895253915 1105 82-83 -1.3159472351916293 1105 84-85 -0.8458753748043719 1105 86-87 -0.6430622548953728 1105 88-89 -0.4373740863497666 1105 90-91 -0.5882410391479151 1105 92-93 -0.40770290079988847 1105 94-95 -0.3998727744672159 1105 96-97 -0.2718212358006866 1105 98-99 -0.21282622606888957 1105 100-101 -0.010719428496463124 1105 102-103 0.14864312485023312 1105 104-105 -0.24149792725512143 1105 106-107 -0.4983722194750069 1105 108-109 -0.6678415256617463 1105 110-111 -0.5097469043849969 1105 112-113 -0.47547261884216674 1105 114-115 -0.504031910923592 1105 116-117 -0.38082580878333694 1105 118-119 -0.5606055826175265 1105 120-121 -0.15896325571215542 1105 122-123 0.27161967491784544 1105 124-125 0.5291313020457125 1106 1 -0.6510743209704088 1106 2 -0.791491855657938 1106 3 0.031679717434244026 1106 4 -0.7375764453680276 1106 5 -0.042172403736000064 1106 6 0.871692206077828 1106 7 -0.6928515685751577 1106 8 -0.9229942928703494 1106 9 0.02820082793041223 1106 10-11 -0.32837869216357163 1106 12-13 0.35243099149380797 1106 14-15 0.32171253896791896 1106 16-17 1.3298853365760657 1106 18-19 1.2330616987569734 1106 20-21 1.7428895130969977 1106 22-23 1.035866911995928 1106 24-25 -1.400040442413097 1106 26-27 0.150276380726023 1106 28-29 -0.03985485377274145 1106 30-31 0.1348297432717338 1106 32-33 -3.1733023477683773 1106 34-35 0.1323211458732132 1106 36-37 1.1073488169221015 1106 38-39 1.880443426115292 1106 40-41 1.483861670679282 1106 42-43 1.6822643005383249 1106 44-45 1.7303931831455621 1106 46-47 0.725512256082574 1106 48-49 0.6992899402911732 1106 50-51 0.8736123221858634 1106 52-53 1.1734306438105033 1106 54-55 0.8610967629529611 1106 56-57 0.5847122728202141 1106 58-59 0.8153669921293201 1106 60-61 1.258047034404612 1106 62-63 0.6109489662071503 1106 64-65 0.2314400600093549 1106 66-67 0.06984028924640207 1106 68-69 0.07091363202396472 1106 70-71 0.8577931577271194 1106 72-73 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3.15587716747752 2214 8 2.4325937810275846 2214 9 2.6466387280584556 2214 10-11 1.1192159666986896 2214 12-13 2.260150033015666 2214 14-15 0.38381241092694296 2214 16-17 0.8489085668668999 2214 18-19 2.153950668941718 2214 20-21 1.6957887265595915 2214 22-23 2.4064540713154834 2214 24-25 2.547828589962979 2214 26-27 1.2753907030234437 2214 28-29 1.3065874363315189 2214 30-31 0.8021975866959146 2214 32-33 0.8749873906622092 2214 34-35 1.0459849468609548 2214 36-37 1.2282560786744305 2214 38-39 1.3965400055921542 2214 40-41 1.2407667374435043 2214 42-43 1.682813047565947 2214 44-45 1.123021681407863 2214 46-47 1.8102936718499052 2214 48-49 2.3552255539732663 2214 50-51 2.4136708552021418 2214 52-53 2.552157550706424 2214 54-55 1.3785652099988752 2214 56-57 1.2983577822403713 2214 58-59 1.8096966061772406 2214 60-61 1.3501450772068821 2214 62-63 1.3830360340688657 2214 64-65 1.5234649365412771 2214 66-67 1.6524267169032516 2214 68-69 1.4572315394686317 2214 70-71 1.3828526053217782 2214 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-5.318696757586425 2216 70-71 -4.938743638809678 2216 72-73 -4.612405976299076 2216 74-75 -4.542386987425537 2216 76-77 -4.316996425336113 2216 78-79 -4.187527156366414 2216 80-81 -4.312321462999863 2216 82-83 -4.536578202397941 2216 84-85 -4.674850053094117 2216 86-87 -4.47712203073597 2216 88-89 -4.72130932918912 2216 90-91 -4.133591807101423 2216 92-93 -3.9782716015055755 2216 94-95 -4.133164596800134 2216 96-97 -3.795897616041451 2216 98-99 -3.5811325772519513 2216 100-101 -3.4035172699244427 2216 102-103 -3.1097628527338266 2216 104-105 -3.2164875495050183 2216 106-107 -3.362112360612409 2216 108-109 -3.6027730325110614 2216 110-111 -3.80437122983124 2216 112-113 -3.632695532914397 2216 114-115 -3.574808166631353 2216 116-117 -3.328937058264448 2216 118-119 -3.177770381289175 2216 120-121 -3.097734530099869 2216 122-123 -2.6655949369542995 2216 124-125 -2.0188139034337382 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 8617.0 3 1724.0 4 2598.0 5 3742.0 6 4800.0 7 2117.0 8 1173.0 9 817.0 10 730.0 11 573.0 12 496.0 13 399.0 14 291.0 15 316.0 16 347.0 17 326.0 18 305.0 19 321.0 20 333.0 21 314.0 22 362.0 23 542.0 24 720.0 25 624.0 26 1010.0 27 565.0 28 597.0 29 510.0 30 551.0 31 699.0 32 784.0 33 933.0 34 1164.0 35 1383.0 36 1777.0 37 2703.0 38 1330.0 39 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.27565932061543 30.147421729147446 6.4075878200038625 9.169331130233257 2 10.27016587626875 53.29499366966374 20.885817900903415 15.549022553164097 3 15.4031029377052 63.30765434218149 13.087701980644193 8.20154073946911 4 5.478423209802365 39.85751378725779 16.05115770047853 38.61290530246132 5 4.987017445977554 73.01130877019807 14.036179481127014 7.965494302697366 6 12.476127121735585 55.72198021501684 11.572713031909187 20.229179631338383 7 14.463208944014077 67.51786442351022 8.519130490762 9.499796141713697 8 13.115598377717216 65.87841462629558 10.05772408317418 10.94826291281303 9 14.926718310765864 55.44087036758868 7.040621445891719 22.59178987575374 10-11 12.919250659857084 62.844144975429714 10.912855947297269 13.323748417415935 12-13 16.595137443402503 61.83236411235811 10.346344499045085 11.226153945194309 14-15 15.691723353576103 60.98259693997983 10.211154267075814 13.114525439368254 16-17 15.241089247011866 60.85384433810434 12.182142014119869 11.722924400763931 18-19 16.807579236497073 58.274500547198556 14.362352739211607 10.555567477092765 20-21 21.220574665779704 53.327181820132616 13.758288448745734 11.693955065341946 22-23 18.88 54.90255033557047 14.319463087248321 11.897986577181207 24-25 20.427673225896438 54.401193107444044 13.852707023454434 11.318426643205083 26-27 21.14010428960752 54.72736636552864 13.32589429411386 10.806635050749984 28-29 17.69382631274007 57.199416321538166 14.49539709448295 10.611360271238816 30-31 17.187399412029787 58.80345915323706 14.420291410055578 9.588850024677582 32-33 16.39771678719341 60.09849574043476 13.892405742366043 9.611381730005794 34-35 14.78616338705178 60.66178837364005 14.094118151970989 10.457930087337182 36-37 15.524344971137957 60.850625523057445 14.468573635758888 9.156455870045708 38-39 16.247505418338662 61.79159245509753 13.353790691186884 8.607111435376922 40-41 14.188536726679684 63.363447136327544 13.670307504130813 8.777708632861955 42-43 12.76045578421064 65.45353104010644 13.037273878242955 8.74873929743997 44-45 11.759404304628656 66.94169653011738 12.232570116521105 9.066329048732861 46-47 11.649964593034484 66.42239436921955 12.144589171906182 9.783051865839788 48-49 11.546962511534087 66.43419669105813 12.346301581511126 9.672539215896654 50-51 11.60060942898221 66.9180918864402 12.39994849895925 9.081350185618334 52-53 10.632819038218063 66.5178858822772 12.335572198021502 10.513722881483229 54-55 10.317375163623097 65.6262741142894 12.259393575245166 11.796957146842342 56-57 11.191819918027509 63.980386686980964 11.246539773824596 13.581253621166928 58-59 11.492242655737002 61.57271303190919 11.540524881440312 15.394519430913501 60-61 12.325915752880839 59.68648741443317 12.30874873929744 15.678848093388552 62-63 14.18209909658591 58.33029334134461 12.343082766464239 15.144524795605244 64-65 14.202484925216197 57.91828501534302 12.949292933628035 14.929937125812751 66-67 14.729297654556769 57.24340679384563 13.200360507285252 14.826935044312354 68-69 14.962125276281624 57.53095427136756 12.82590502349735 14.681015428853458 70-71 14.63380614149911 56.66831183880174 13.355936567884811 15.34194545181434 72-73 15.115555460183257 55.29816956717667 13.563013669234566 16.023261303405505 74-75 15.606961224008067 54.01064354842171 15.42456170468445 14.957833522885775 76-77 16.689556018111197 52.1812836634407 16.957790605351818 14.171369713096285 78-79 15.988927276238707 51.98064419218472 18.178794446471105 13.851634085105468 80-81 16.34299693139632 52.32291152550375 17.959915023282765 13.374176519817171 82-83 16.352653376536985 51.242462608098535 17.58009484775005 14.82478916761443 84-85 16.307589965880563 50.36265316194931 17.948112701444174 15.38164417072595 86-87 17.680951052552523 48.303684470290335 18.40089268470634 15.614471792450807 88-89 18.05969828973627 47.17173451213493 18.661616703504215 16.106950494624577 90-91 17.791463702495655 46.50543979742924 19.394433595845584 16.308662904229525 92-93 17.18525353533186 47.70283899487136 18.410549129847 16.70135833994979 94-95 16.26681830861999 49.03972017767859 17.758202613677817 16.935258900023605 96-97 15.400957061007276 50.38840368232441 16.821527435033584 17.389111821634728 98-99 14.953541769489926 52.75959743353147 15.804381880217164 16.48247891676144 100-101 13.237913349498937 56.27990815647733 15.323705499881976 15.158472994141755 102-103 11.079161391386451 60.63067316152014 14.723932962811956 13.566232484281453 104-105 10.033046501148043 63.44928220424454 13.878457543829532 12.63921375077788 106-107 9.072766678826634 66.3977167871934 13.299070835389799 11.23044569859016 108-109 8.724061715413832 67.66914873071393 12.582348018282868 11.024441535589364 110-111 8.882856591060277 68.153043926096 12.187506705864681 10.776592776979035 112-113 9.468680929593786 68.46312310894615 11.829145297311216 10.239050664148838 114-115 9.534130168880496 68.10798051543958 11.741164352696295 10.616724962983627 116-117 9.607089976609943 67.02645865968542 12.279779403875454 11.086671959829188 118-119 9.950430248277934 66.51895882062617 12.221840733031481 11.308770198064419 120-121 10.834531447823007 64.84517499624471 12.460033046501149 11.860260509431129 122-123 11.188601102980622 63.023325679706446 12.576983326538057 13.211089890774877 124-125 11.741164352696295 63.29263320529602 12.587712710027683 12.37848973198 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1303.0 1 2022.0 2 2656.5 3 2103.5 4 1281.0 5 790.0 6 585.0 7 508.0 8 502.5 9 472.5 10 399.0 11 327.5 12 282.5 13 246.5 14 211.5 15 186.5 16 170.0 17 157.5 18 158.5 19 170.0 20 189.5 21 222.5 22 243.0 23 270.5 24 322.5 25 432.5 26 535.0 27 602.0 28 721.5 29 848.0 30 998.5 31 1167.5 32 1327.0 33 1394.5 34 1458.0 35 1602.0 36 1711.0 37 1643.0 38 1495.5 39 1411.5 40 1262.5 41 1108.0 42 1030.5 43 915.0 44 799.5 45 746.0 46 732.5 47 774.5 48 1092.5 49 1117.0 50 914.0 51 811.0 52 526.0 53 378.0 54 312.0 55 248.0 56 198.0 57 158.5 58 126.5 59 119.5 60 91.0 61 58.0 62 53.5 63 55.0 64 50.0 65 43.5 66 41.5 67 48.0 68 43.5 69 31.0 70 25.5 71 26.0 72 35.0 73 42.5 74 37.5 75 18.0 76 11.5 77 11.0 78 12.0 79 10.0 80 2.5 81 1.0 82 0.5 83 1.0 84 1.5 85 1.0 86 1.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.08261625287011008 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 46601.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.3551211347396 #Duplication Level Percentage of deduplicated Percentage of total 1 83.31522647501254 42.78663547992532 2 7.466989804445929 7.669363318383726 3 3.555908407153602 5.478423209802365 4 1.9847902390105299 4.077165726057381 5 1.2159451780043458 3.122250595480784 6 0.6560254053150593 2.0214158494452907 7 0.4303860939328097 1.5471770992038798 8 0.2757813805783052 1.133022896504367 9 0.19638977101788402 0.9077058432222485 >10 0.7437740264081565 7.01487092551662 >50 0.07521310379408325 2.5750520375099244 >100 0.058499080728731404 5.768116564022232 >500 0.012535517299013872 4.693032338361838 >1k 0.012535517299013872 11.205768116564021 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2051 4.401193107444047 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1742 3.738117207785241 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1429 3.0664578013347352 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 892 1.914122014549044 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 792 1.69953434475655 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 503 1.0793759790562434 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 438 0.9398939936911226 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 367 0.787536748138452 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 280 0.6008454754189824 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 204 0.4377588463766872 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 187 0.4012789425119632 No Hit AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 168 0.3605072852513895 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 164 0.3519237784596897 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 162 0.34763202506383983 No Hit ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 152 0.32617325808459047 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 137 0.2939851076157164 No Hit AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 114 0.24462994356344286 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 111 0.23819231346966802 No Hit AAAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 102 0.2188794231883436 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 102 0.2188794231883436 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 97 0.20815003969871892 No Hit AAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 85 0.18239951932361967 No Hit AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 82 0.17596188922984485 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81 0.17381601253191992 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 81 0.17381601253191992 No Hit TAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.16523250574022016 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 65 0.13948198536512094 No Hit GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 65 0.13948198536512094 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 63 0.13519023196927105 No Hit AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 62 0.13304435527134612 No Hit GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 61 0.13089847857342116 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 59 0.1266067251775713 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 58 0.12446084847964635 No Hit GAACAAAAAAACACAAAAAACATGGGGGGGAGGTAGTGACGAAAAAAAAA 56 0.12016909508379649 No Hit AAAAAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 53 0.11373146499002167 No Hit GAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 52 0.11158558829209672 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 52 0.11158558829209672 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 51 0.1094397115941718 No Hit GAACAAAAAAACACAAAGAACATGGGGGGGAGGTAGTGACGAAAAAAAAA 47 0.10085620480247204 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0010729383489624686 0.0 22-23 0.0 0.0 0.0 0.006437630093774812 0.0 24-25 0.0 0.0 0.0 0.01502113688547456 0.0 26-27 0.0 0.0 0.0 0.024677582026136777 0.0 28-29 0.0 0.0 0.0 0.028969335421986653 0.0 30-31 0.0 0.0 0.0 0.06223042423982318 0.0 32-33 0.0 0.0 0.0 0.12767966352653376 0.0 34-35 0.0 0.0 0.0 0.20707710134975643 0.0 36-37 0.0 0.0 0.0 0.28969335421986653 0.0 38-39 0.0 0.0 0.0 0.3894766206733761 0.0 40-41 0.0 0.0 0.0 0.5407609278770842 0.0 42-43 0.0 0.0 0.0 0.6652217763567305 0.0 44-45 0.0 0.0 0.0 0.7843179330915646 0.0 46-47 0.0 0.0 0.0 0.8916117679878114 0.0 48-49 0.0 0.0 0.0 1.0160726164674578 0.0 50-51 0.0 0.0 0.0 1.0976159309886053 0.0 52-53 0.0 0.0 0.0 1.1941803823952277 0.0 54-55 0.0 0.0 0.0 1.300401278942512 0.0 56-57 0.0 0.0 0.0 1.4473938327503701 0.0 58-59 0.0 0.0 0.0 1.6126263384905903 0.0 60-61 0.0 0.0 0.0 1.7403060020171242 0.0 62-63 0.0 0.0 0.0 1.8583292204029958 0.0 64-65 0.0 0.0 0.0 1.946310165017918 0.0 66-67 0.0 0.0 0.0 2.043947554773503 0.0 68-69 0.0 0.0 0.0 2.1555331430655995 0.0 70-71 0.0 0.0 0.0 2.335786785691294 0.0 72-73 0.0 0.0 0.0 2.4548829424261283 0.0 74-75 0.0 0.0 0.0 2.576124975858887 0.0 76-77 0.0 0.0 0.0 2.7209716529688204 0.0 78-79 0.0 0.0 0.0 2.8668912684277164 0.0 80-81 0.0 0.0 0.0 2.9816956717667003 0.0 82-83 0.0 0.0 0.0 3.1286882255745585 0.0 84-85 0.0 0.0 0.0 3.32503594343469 0.0 86-87 0.0 0.0 0.0 3.5310401064354844 0.0 88-89 0.0 0.0 0.0 3.720950194201841 0.0 90-91 0.0 0.0 0.0 3.895839145082723 0.0 92-93 0.0 0.0 0.0 4.082530417802193 0.0 94-95 0.0 0.0 0.0 4.2370335400527885 0.0 96-97 0.0 0.0 0.0 4.417287182678484 0.0 98-99 0.0 0.0 0.0 4.582519688418703 0.0 100-101 0.0 0.0 0.0 4.843243707216583 0.0 102-103 0.0 0.002145876697924937 0.0 5.056758438660115 0.0 104-105 0.0 0.002145876697924937 0.0 5.239157957983734 0.0 106-107 0.0 0.002145876697924937 0.0 5.439797429239716 0.0 108-109 0.0 0.002145876697924937 0.0 5.6415098388446605 0.0 110-111 0.0 0.002145876697924937 0.0 5.940859638205189 0.0 112-113 0.0 0.002145876697924937 0.0 6.216604793888543 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACAAGG 40 1.546141E-10 104.11843 2 GTACATA 50 9.094947E-12 95.193985 1 TACAAGA 25 1.7088547E-5 95.193985 2 TACATGA 40 1.8613719E-8 89.24437 2 TACATAA 50 9.113137E-10 83.29474 2 GGAAACA 120 0.0 79.32832 1 CGCAGAA 30 4.222692E-5 79.32832 2 GAACAAA 520 0.0 74.37031 1 GGAATCA 50 8.747156E-8 71.39549 1 GTACAAA 125 0.0 71.39549 1 CATAGGA 25 0.0020449592 71.39549 6 ACATAGG 35 9.063628E-5 67.995705 5 GAAAACA 405 0.0 63.16885 1 GAGAACA 370 0.0 61.10425 1 AGTGCCA 15 0.0042254203 59.496246 104-105 TCTGCTG 15 0.0042254203 59.496246 66-67 AGAACAA 250 0.0 59.496243 2 GAAACAA 220 0.0 56.79187 2 ACAACAC 305 0.0 56.5702 5 GAATCAA 65 5.369984E-7 54.919613 2 >>END_MODULE