FastQCFastQC Report
Thu 26 May 2016
SRR936193_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936193_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306870
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC6810.2219180760582657No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA6720.2189852380486851No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG6700.21833349626877832No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG6650.2167041418190113No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT6130.1997588555414345No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT5730.18672401994329846No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC5030.16391305764656044No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG4930.16065434874702642No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT4590.14957473848861083No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT4510.14696777136898362No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC4440.1446866751393098No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT4440.1446866751393098No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG4440.1446866751393098No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC4390.1430573206895428No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT4370.142405578909636No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA4340.1414279662397758No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC4230.13784338645028837No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT4070.132629452211034No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA4020.13100009776126698No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG3770.12285332551243197No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT3760.12252745462247858No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC3740.12187571284257177No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG3730.12154984195261838No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA3690.12024635839280479No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT3680.11992048750285138No Hit
GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC3630.11829113305308436No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC3520.11470655326359697No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT3500.11405481148369016No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG3480.11340306970378336No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC3360.10949261902434256No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA3360.10949261902434256No Hit
GCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTT3330.10851500635448234No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC3280.10688565190471536No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC3230.10525629745494836No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT3230.10525629745494836No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA3220.10493042656499496No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA3190.10395281389513475No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT3190.10395281389513475No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG3170.10330107211522793No Hit
ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT3130.10199758855541435No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA3120.10167171766546095No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG3070.10004236321569394No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA208.4801466E-489.238022
GTTACCA359.147829E-567.9908759
CGTTTTC201.7525992E-459.6280420-21
GCGGAGT300.00423584859.4920126
GGTATCA4900.053.4214061
CATTCCG1155.456968E-1251.732199
CCCGGGT350.00779497350.9931563
GACATAT1700.048.9934271
GTGGTAC505.3299824E-447.593611
GGGTATG909.703217E-846.271577
TAGGCAT1301.8189894E-1145.763095
CCTTTAG1204.1654857E-1044.619011
GGCACAT558.524253E-443.266925
TTCGATA558.524253E-443.266924
GTACATG19800.042.6659931
AGGCTTA1700.041.9943666
CATGGGT2000.041.644414
ATGGGAT6800.041.1194845
GTATAGG2750.041.1035731
TTAGGCA1455.820766E-1141.0289764
Produced by FastQC (version 0.11.5)