##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936193_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 306870 Sequences flagged as poor quality 0 Sequence length 125 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.888483722749047 31.0 30.0 34.0 26.0 34.0 2 29.929901912862125 31.0 30.0 34.0 26.0 34.0 3 30.012689412454787 31.0 30.0 34.0 26.0 34.0 4 33.53825072506273 35.0 35.0 37.0 28.0 37.0 5 33.8027829374002 37.0 35.0 37.0 30.0 37.0 6 33.82185290188028 37.0 35.0 37.0 29.0 37.0 7 33.698931143480955 37.0 35.0 37.0 29.0 37.0 8 33.60601883533744 37.0 35.0 37.0 28.0 37.0 9 34.99815231205397 39.0 35.0 39.0 27.0 39.0 10-11 34.90890768077688 39.0 35.0 39.0 27.0 39.0 12-13 35.00830481963047 39.0 35.0 39.0 27.0 39.0 14-15 36.117715970932316 40.0 36.0 41.0 27.0 41.0 16-17 36.11734447811777 40.0 36.0 41.0 27.0 41.0 18-19 36.21660149248868 40.0 36.0 41.0 27.5 41.0 20-21 36.34883827027732 40.0 36.0 41.0 28.0 41.0 22-23 36.2478981327598 40.0 36.0 41.0 27.5 41.0 24-25 36.25307785055561 39.0 36.0 41.0 28.0 41.0 26-27 36.348610160654346 39.5 36.0 41.0 29.0 41.0 28-29 36.26087919966109 39.0 36.0 41.0 29.0 41.0 30-31 36.200431778929186 39.0 36.0 41.0 29.0 41.0 32-33 36.03227751164988 39.0 35.0 41.0 28.5 41.0 34-35 36.019544106624956 39.0 35.0 41.0 28.0 41.0 36-37 35.904995600742986 39.0 35.0 41.0 28.0 41.0 38-39 35.65563430768729 39.0 35.0 41.0 27.0 41.0 40-41 35.78543357121909 39.0 35.0 41.0 27.5 41.0 42-43 35.71720435363509 39.0 35.0 41.0 27.0 41.0 44-45 35.58793625965393 39.0 35.0 41.0 27.0 41.0 46-47 35.611361488578225 39.0 35.0 41.0 27.0 41.0 48-49 35.553138136670256 39.0 35.0 41.0 27.0 41.0 50-51 35.46962720370189 39.0 35.0 40.5 27.0 41.0 52-53 35.399051715710236 39.0 35.0 40.0 27.0 41.0 54-55 35.25308273861896 39.0 34.0 40.0 27.0 41.0 56-57 35.110092221461855 39.0 34.0 40.0 27.0 41.0 58-59 34.90691172157591 38.5 34.0 40.0 26.0 41.0 60-61 34.724850914067844 38.0 34.0 40.0 26.0 41.0 62-63 34.51357089321211 38.0 34.0 40.0 26.0 41.0 64-65 34.25791866262587 38.0 33.0 40.0 25.5 41.0 66-67 33.979471763287385 37.5 33.0 40.0 25.0 41.0 68-69 33.690574184508094 37.0 33.0 39.5 24.5 41.0 70-71 33.365200247661875 36.5 32.5 39.0 23.0 41.0 72-73 33.01982598494476 36.0 32.0 39.0 22.5 40.0 74-75 32.61038224655391 36.0 32.0 38.5 22.0 40.0 76-77 32.26839541173787 35.0 32.0 37.5 22.0 39.5 78-79 31.88237690227132 35.0 31.5 37.0 20.5 39.0 80-81 31.522349854987453 35.0 31.0 36.5 20.0 39.0 82-83 31.156626584547205 35.0 31.0 36.0 19.5 37.5 84-85 31.072978785805063 35.0 31.0 36.0 20.0 37.0 86-87 30.943021474891648 35.0 32.0 35.5 19.5 37.0 88-89 30.72574868836967 35.0 31.0 35.0 18.5 36.0 90-91 30.533787923224818 35.0 31.0 35.0 18.0 36.0 92-93 30.4056049793072 35.0 31.0 35.0 17.0 36.0 94-95 30.268152637924853 34.0 31.0 35.0 12.5 36.0 96-97 30.154327565418583 34.0 31.0 35.0 10.5 35.0 98-99 30.061656401733632 34.0 31.0 35.0 7.5 35.0 100-101 29.983898719327403 34.0 31.0 35.0 7.0 35.0 102-103 29.01936324828103 33.5 29.5 34.5 7.0 35.0 104-105 29.701606543487472 34.0 30.5 35.0 3.5 35.0 106-107 29.82679147521752 34.0 31.0 35.0 2.0 35.0 108-109 29.783911754163 34.0 31.0 35.0 2.0 35.0 110-111 29.684123570241468 34.0 31.0 35.0 2.0 35.0 112-113 29.658928862384723 34.0 31.0 35.0 2.0 35.0 114-115 29.579831850620785 34.0 31.0 35.0 2.0 35.0 116-117 29.454249356404993 34.0 31.0 35.0 2.0 35.0 118-119 29.276064783132924 34.0 31.0 35.0 2.0 35.0 120-121 29.096623977580084 34.0 30.0 35.0 2.0 35.0 122-123 28.831832697885098 34.0 30.0 35.0 2.0 35.0 124-125 26.789997393032877 32.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5523726150350008 1101 2 0.607217057965908 1101 3 0.6829342481662657 1101 4 0.5528516654407696 1101 5 0.6718252481049305 1101 6 0.7986635488293246 1101 7 0.5385252231049478 1101 8 0.7339185015987297 1101 9 0.5373695597960761 1101 10-11 0.7288824978232356 1101 12-13 0.44820933453453904 1101 14-15 0.538983243852158 1101 16-17 0.6417322446141398 1101 18-19 0.34626313191891 1101 20-21 0.169098759949172 1101 22-23 0.6160769440713452 1101 24-25 0.8325174610183836 1101 26-27 0.6882984062284763 1101 28-29 0.6014547374299752 1101 30-31 0.5928201433739417 1101 32-33 0.6089666217629599 1101 34-35 0.628765441516272 1101 36-37 0.8199551233731981 1101 38-39 0.8887921007171613 1101 40-41 0.6433139569543371 1101 42-43 0.7855601590976562 1101 44-45 0.6771237440553364 1101 46-47 0.5743457744918956 1101 48-49 0.5473598595164191 1101 50-51 0.6802685443643739 1101 52-53 0.7039848213636759 1101 54-55 0.6981717253180193 1101 56-57 0.4213972925536069 1101 58-59 0.36341147909769234 1101 60-61 0.4367679487948095 1101 62-63 0.4363490219816697 1101 64-65 0.5771228439005256 1101 66-67 0.777981112848039 1101 68-69 0.5755732681159245 1101 70-71 0.5572031769047499 1101 72-73 0.49250276065175314 1101 74-75 0.5113444094089701 1101 76-77 0.44734469202470706 1101 78-79 0.5414656559981985 1101 80-81 0.6336926255105979 1101 82-83 0.5041553793178224 1101 84-85 0.3228587287289315 1101 86-87 0.2738752077838633 1101 88-89 0.15092466853761266 1101 90-91 0.19550347584054606 1101 92-93 0.24805886615167694 1101 94-95 0.4995313691111747 1101 96-97 0.36335763061954296 1101 98-99 0.3853411698924347 1101 100-101 0.4636954499436854 1101 102-103 0.6244117869131109 1101 104-105 0.6052010499616785 1101 106-107 0.25199035774159384 1101 108-109 0.24933289708490136 1101 110-111 0.3702369551012694 1101 112-113 0.4237835880683427 1101 114-115 0.48513469093774475 1101 116-117 0.35427427235064 1101 118-119 0.37984511633597506 1101 120-121 0.4735993494360713 1101 122-123 0.5792780268463424 1101 124-125 0.34157040899670577 1102 1 -0.3415961920000079 1102 2 -0.21913104224978497 1102 3 -0.18356649847385498 1102 4 -0.2344056765204101 1102 5 -0.328124975887107 1102 6 -0.38441260846285985 1102 7 -0.4705588983485143 1102 8 -0.4379829419055028 1102 9 -0.566488080821145 1102 10-11 -0.3653600772555734 1102 12-13 -0.6528981816426693 1102 14-15 -0.16425219666550817 1102 16-17 -0.4886269722433312 1102 18-19 -0.43804912957104847 1102 20-21 -0.42560839120397276 1102 22-23 -0.07717011084818637 1102 24-25 -0.11594363073538716 1102 26-27 -0.25222756025568316 1102 28-29 -0.35505347561134215 1102 30-31 -0.2986142352555561 1102 32-33 -0.20871215706564783 1102 34-35 -0.22854748365379862 1102 36-37 -0.2636976060112346 1102 38-39 -0.42313754919158697 1102 40-41 -0.45574859489566677 1102 42-43 -0.09591486993834764 1102 44-45 -0.20129420849153945 1102 46-47 -0.35624573040274043 1102 48-49 -0.4693690271602122 1102 50-51 -0.6568394695729083 1102 52-53 -0.5627813973189149 1102 54-55 -0.6549351271790727 1102 56-57 -0.6895575771991673 1102 58-59 -0.33531093669789414 1102 60-61 -0.6236725688756692 1102 62-63 -0.5404387663043764 1102 64-65 -0.5980223361227104 1102 66-67 -0.6841144570872473 1102 68-69 -0.7672216547312658 1102 70-71 -0.9817216613232347 1102 72-73 -0.5593223596402623 1102 74-75 -0.7217068598792373 1102 76-77 -0.5425715183618856 1102 78-79 -0.6230606423260063 1102 80-81 -0.633936377310043 1102 82-83 -0.4594417668577222 1102 84-85 -0.5907384174466124 1102 86-87 -0.4853209096875162 1102 88-89 -0.5515392438566167 1102 90-91 -0.45859060081450664 1102 92-93 -0.5288243261496355 1102 94-95 -0.7595486043416173 1102 96-97 -0.5956559748226304 1102 98-99 -0.6035961123375309 1102 100-101 -0.569202343319958 1102 102-103 -0.6085814103657974 1102 104-105 -0.6382658489930293 1102 106-107 -0.5159331531261664 1102 108-109 -0.4789647634427254 1102 110-111 -0.5773489085124694 1102 112-113 -0.8981800754458433 1102 114-115 -0.616362737301845 1102 116-117 -0.5737993961411476 1102 118-119 -0.5835031497997463 1102 120-121 -0.4104972160193796 1102 122-123 -0.4911757644262664 1102 124-125 -0.7422731284182582 1103 1 -0.06079327535080026 1103 2 0.007060132372011196 1103 3 0.1645288793076496 1103 4 -0.34733364031372815 1103 5 -0.06008239951364658 1103 6 0.17490935440852695 1103 7 0.14298508187191317 1103 8 0.25326291988891114 1103 9 0.28228617220655394 1103 10-11 0.10527437775846238 1103 12-13 0.22519761519762937 1103 14-15 0.17868875795042527 1103 16-17 -0.026522458980124952 1103 18-19 0.17371175548111495 1103 20-21 0.04945551526210323 1103 22-23 0.13049656978710544 1103 24-25 0.2516861727260391 1103 26-27 0.01449079032889955 1103 28-29 0.016295474646383923 1103 30-31 0.08407863655436643 1103 32-33 0.3683988516088732 1103 34-35 0.28388180017472564 1103 36-37 0.2055309067491038 1103 38-39 0.32901012712740396 1103 40-41 0.1841730411098652 1103 42-43 0.3927328273335817 1103 44-45 0.5856206806674109 1103 46-47 0.5831248432887506 1103 48-49 0.1064093745495569 1103 50-51 0.3246067023250063 1103 52-53 0.497026673753453 1103 54-55 0.5481596220142393 1103 56-57 0.41694202469497554 1103 58-59 0.12021854408826016 1103 60-61 0.550649603525116 1103 62-63 0.5517185483668712 1103 64-65 0.32906771961211234 1103 66-67 0.4739419482264182 1103 68-69 0.47768049504035304 1103 70-71 0.42027724912390596 1103 72-73 0.1953040494449283 1103 74-75 0.2921702720823802 1103 76-77 0.36233985070320074 1103 78-79 0.24619929984388378 1103 80-81 0.34191535968865594 1103 82-83 0.2286800116277945 1103 84-85 0.3425578990265912 1103 86-87 0.2096755283127365 1103 88-89 0.27511708602646934 1103 90-91 0.47113952532219017 1103 92-93 0.22715145225207678 1103 94-95 0.16149652829048833 1103 96-97 0.3719989721664305 1103 98-99 0.2812381499094627 1103 100-101 0.23002030261726603 1103 102-103 0.2144186315400809 1103 104-105 0.2217370844185318 1103 106-107 0.18329158810502477 1103 108-109 0.19766013703081242 1103 110-111 0.24694602506670904 1103 112-113 0.3880433667030907 1103 114-115 0.2934830490619831 1103 116-117 0.1383805477049762 1103 118-119 0.2158787384791765 1103 120-121 0.11781230080583072 1103 122-123 -0.029666314650459213 1103 124-125 -0.004339926390017013 1104 1 -0.35819649065605574 1104 2 -0.04522196642433585 1104 3 -0.18901697134592865 1104 4 -0.032514188217767526 1104 5 -0.2753292234397904 1104 6 -0.12816571946336097 1104 7 -0.39440160616334197 1104 8 -0.39006523823866956 1104 9 0.16745086060908676 1104 10-11 2.2396123787160604E-4 1104 12-13 -0.105652454083355 1104 14-15 0.04812958531557854 1104 16-17 -0.06355230823138669 1104 18-19 -0.1964197949103621 1104 20-21 -0.2607792888313156 1104 22-23 -0.2060775274733757 1104 24-25 -0.37683213247754566 1104 26-27 -0.39605118726746724 1104 28-29 -0.19224444956189757 1104 30-31 -0.14295221434963423 1104 32-33 0.058153613632882184 1104 34-35 0.1297817016788727 1104 36-37 -0.0103294294723284 1104 38-39 0.1743612064082143 1104 40-41 0.04473672118197669 1104 42-43 -0.08334227992673249 1104 44-45 -0.41332341041621135 1104 46-47 -0.19699568892273334 1104 48-49 -0.08983526243480355 1104 50-51 -0.2323330816518876 1104 52-53 -0.3823972924574548 1104 54-55 -0.07418599825921035 1104 56-57 -0.03591978061712098 1104 58-59 -0.10711697618685889 1104 60-61 -0.10083367722144487 1104 62-63 -0.28113065281507943 1104 64-65 -0.26840561138402563 1104 66-67 -0.5116530334934168 1104 68-69 -0.39190640668082466 1104 70-71 -0.19889438407086857 1104 72-73 0.2303076387005376 1104 74-75 -0.19495640359916422 1104 76-77 -0.17765530797529294 1104 78-79 0.09634370477869325 1104 80-81 -0.029925260668264286 1104 82-83 -0.08832429547892673 1104 84-85 -0.07246647452310029 1104 86-87 0.09704593949118134 1104 88-89 0.07673767666769038 1104 90-91 0.2971294921007086 1104 92-93 0.09155480111102676 1104 94-95 0.262742751224998 1104 96-97 0.1733169802130412 1104 98-99 0.06623547883552305 1104 100-101 0.023654799537183635 1104 102-103 0.16201341292937244 1104 104-105 0.09300592801045582 1104 106-107 0.005039138229395945 1104 108-109 0.2422190759466929 1104 110-111 0.1558060607923224 1104 112-113 0.12906814091387275 1104 114-115 -0.18153197572891955 1104 116-117 -0.07052247561684055 1104 118-119 0.027202839913208265 1104 120-121 0.25103837382631156 1104 122-123 0.1666764008300845 1104 124-125 -0.0822100788081741 1105 1 0.12361017567944899 1105 2 0.01769509349982812 1105 3 0.14465994485296818 1105 4 -0.07826452352153268 1105 5 -0.1132822974715495 1105 6 -0.16336019564437265 1105 7 0.10527550873408131 1105 8 -0.1532828296813875 1105 9 -0.011398509351010944 1105 10-11 0.13552316809959564 1105 12-13 0.03065059428318051 1105 14-15 0.017002219561263132 1105 16-17 -0.002235185386027183 1105 18-19 0.022624630768113718 1105 20-21 -0.16787324011706062 1105 22-23 0.06089256839866408 1105 24-25 0.07208371869725738 1105 26-27 0.03671212967324067 1105 28-29 -0.06167054198982669 1105 30-31 -0.15188743863059528 1105 32-33 0.3740759887425753 1105 34-35 0.22083944015849966 1105 36-37 -0.04714247071883193 1105 38-39 0.33803290677084163 1105 40-41 0.15243753087847267 1105 42-43 0.1155777108006717 1105 44-45 0.05652822801870627 1105 46-47 -0.11658074017039866 1105 48-49 -0.2354274668293499 1105 50-51 -0.2807120328548436 1105 52-53 0.018938541165638867 1105 54-55 0.00975791908220458 1105 56-57 -0.20168609459391007 1105 58-59 -0.5239347365233229 1105 60-61 -0.6229223298798701 1105 62-63 -0.4476896911142205 1105 64-65 -0.2052815738300069 1105 66-67 -0.16339040371844504 1105 68-69 -0.0878376404330794 1105 70-71 -0.02026441039841842 1105 72-73 -0.047104478597830735 1105 74-75 0.36908868440772125 1105 76-77 0.2736594139295647 1105 78-79 0.3855411283211545 1105 80-81 0.3580936322497905 1105 82-83 0.38331769945425975 1105 84-85 0.18216361505681888 1105 86-87 0.22957164611280945 1105 88-89 0.32993962060159276 1105 90-91 0.4037457390167454 1105 92-93 0.4510397579768899 1105 94-95 0.34021447808076033 1105 96-97 0.28706774603027085 1105 98-99 0.2579692062542662 1105 100-101 0.2996222129791377 1105 102-103 0.21232760497149883 1105 104-105 0.22607805557490934 1105 106-107 0.22573524539413725 1105 108-109 0.26372612146521135 1105 110-111 0.06797924311477743 1105 112-113 -0.011819461954008403 1105 114-115 6.807807163617952E-4 1105 116-117 0.16053079214598043 1105 118-119 0.12674873099330242 1105 120-121 0.16534342573630312 1105 122-123 0.21469314118078486 1105 124-125 0.47743259501164204 1106 1 -0.2733581925748858 1106 2 -0.18095915377608662 1106 3 -0.1672399678616081 1106 4 0.1069567839567398 1106 5 -0.2206515639871398 1106 6 -0.10924733108430473 1106 7 -0.43606622804094997 1106 8 -0.08282341330532716 1106 9 -0.4641885694673107 1106 10-11 -0.12987574267667412 1106 12-13 -0.13004678756391286 1106 14-15 -0.22165374883567068 1106 16-17 -0.1259940317431898 1106 18-19 -0.3578967869438614 1106 20-21 -0.24686449928100274 1106 22-23 -0.3276300131189558 1106 24-25 -3.472583469769596 1106 26-27 -1.0407874912703434 1106 28-29 -0.7205320614118591 1106 30-31 -0.7175277161408644 1106 32-33 -5.519491116536759 1106 34-35 -1.2294672881953375 1106 36-37 -0.4602770828724019 1106 38-39 -0.4691277762683299 1106 40-41 -0.813222006795641 1106 42-43 -0.3244862985097754 1106 44-45 -0.5923692300494565 1106 46-47 -0.6203733532828792 1106 48-49 -0.3354512346911065 1106 50-51 -0.278066833758011 1106 52-53 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-0.8165507470470956 2216 34-35 -0.6716133463625198 2216 36-37 -0.6385352683488961 2216 38-39 -0.2827618534587444 2216 40-41 -0.49999720342999154 2216 42-43 -0.4473759088993816 2216 44-45 -0.22070515468819707 2216 46-47 -0.34550825359384163 2216 48-49 -0.6836083592566808 2216 50-51 -0.8930407682742043 2216 52-53 -1.1314734195823632 2216 54-55 -0.6292857765297271 2216 56-57 -0.4389777998336797 2216 58-59 -0.5916421055290613 2216 60-61 -0.6359870401187067 2216 62-63 -1.2483701206643047 2216 64-65 -0.9282171413175178 2216 66-67 -0.9540587984601601 2216 68-69 -0.6555797252321938 2216 70-71 -0.9221205481506871 2216 72-73 -0.7705700405308065 2216 74-75 -1.05132188770855 2216 76-77 -0.7618201268962075 2216 78-79 -0.36777307275272264 2216 80-81 -0.09720168343248758 2216 82-83 -0.5202256258558684 2216 84-85 -0.5674313673538478 2216 86-87 -0.391257829910149 2216 88-89 -0.5488258857861155 2216 90-91 -0.19917501639892166 2216 92-93 -0.1610135654817313 2216 94-95 -0.5792842258629562 2216 96-97 -0.2010363087743947 2216 98-99 -0.1761610474024664 2216 100-101 -0.09907247319009116 2216 102-103 -0.22520942520809584 2216 104-105 -0.26813597886315677 2216 106-107 -0.5192494796567821 2216 108-109 -0.6336493645558967 2216 110-111 -0.6519082777146963 2216 112-113 -0.6530733964105941 2216 114-115 -0.6923135721812521 2216 116-117 -0.5710860380706464 2216 118-119 -0.7293751349574471 2216 120-121 -0.6841057503421979 2216 122-123 -0.40272492954685646 2216 124-125 -0.882672015245717 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6125.0 3 997.0 4 724.0 5 744.0 6 1121.0 7 1233.0 8 1146.0 9 1080.0 10 1250.0 11 1403.0 12 1522.0 13 1474.0 14 1512.0 15 1461.0 16 1481.0 17 1320.0 18 1097.0 19 954.0 20 927.0 21 868.0 22 947.0 23 1422.0 24 2022.0 25 2926.0 26 3937.0 27 3804.0 28 4405.0 29 5411.0 30 6682.0 31 8356.0 32 10767.0 33 14336.0 34 19983.0 35 29919.0 36 50450.0 37 85210.0 38 27845.0 39 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.79678558859184 22.654366046638945 10.746966122241485 21.801882242527732 2 17.799068009254736 22.945546974288785 38.8063349301007 20.449050086355786 3 15.178088441359533 32.254048945807675 32.35278782546355 20.215074787369243 4 9.885651532086982 19.535371771016298 42.14339017626414 28.435586520632583 5 9.684294224226702 43.00513575869755 37.68982103054082 9.620748986534927 6 25.044644602891147 44.31351590912053 19.301784480623592 11.34005500736473 7 24.84358095337409 36.50527262536335 24.986639206434035 13.664507214828525 8 24.54418363655916 36.792486647309744 21.331391123841925 17.331938592289166 9 26.32354187169644 15.500902674131382 22.19470387726239 35.98085157690979 10-11 21.120036498134358 29.445847522526194 31.628729245759533 17.805386733579915 12-13 22.022224756814886 29.93857233636941 29.85286689586626 18.186336010949443 14-15 21.61169117263674 30.698941550654364 26.218943896970643 21.470423379738254 16-17 20.052564087941384 32.06018311073164 28.1995089021666 19.687743899160377 18-19 19.744903279628762 31.22930679388915 29.412703999165757 19.613085927316334 20-21 20.194353832166144 30.918683581924185 29.647432241749577 19.239530344160098 22-23 20.665659919503714 30.52531327666847 28.786391300660803 20.022635503167013 24-25 20.618024212601632 32.05758232447493 28.643703257132614 18.68069020579082 26-27 19.457904941912567 31.55736235804018 29.393544392485293 19.591188307561957 28-29 19.646360300587226 29.857494802291555 30.568717290283054 19.92742760683816 30-31 20.27764426014485 30.758063333523427 29.511597024774538 19.452695381557188 32-33 19.879035148892353 31.06160343602745 29.943199963501982 19.116161451578215 34-35 20.09108165576348 31.168338126390054 29.43436499466382 19.306215223182644 36-37 20.607265350680667 30.45907435619608 28.580250433819153 20.353409859304097 38-39 19.434251860734907 31.835512337552302 28.95952005422527 19.770715747487518 40-41 19.19694720668434 31.25007128507022 30.729323663547504 18.823657844697937 42-43 19.450151776098075 30.918656810046063 29.6837549879182 19.947436425937664 44-45 19.244950996765706 31.05151246058967 29.73367984553594 19.969856697108685 46-47 20.212894227447812 30.705258972971915 29.201508150137194 19.88033864944308 48-49 19.120179750675774 31.520401994984837 29.640449767735145 19.71896848660424 50-51 18.869117352333994 30.947822587346614 29.68473261173833 20.498327448581062 52-53 19.600054095184714 31.146931972046477 29.40773919603476 19.845274736734055 54-55 19.998826857150306 31.16714678624034 29.26176727452846 19.572259082080894 56-57 19.546972074357583 31.907641220913845 29.121105620159778 19.424281084568797 58-59 19.264929440460655 30.333025383837054 29.80592664284539 20.59611853285691 60-61 19.84859939778667 31.82117392494493 28.973206720805038 19.357019956463365 62-63 19.262159524356523 30.97157251395956 30.172370249797552 19.593897711886363 64-65 19.27636638554688 30.735365042950065 30.310752505963478 19.677516065539578 66-67 19.39804834970028 30.955767698541887 28.936987667355886 20.70919628440195 68-69 19.44797471241894 30.814677224883503 29.67152214292697 20.065825919770585 70-71 19.575553165835696 30.639032815198618 30.353569915599437 19.431844103366245 72-73 19.785739889855638 30.684654739792094 28.998435819728225 20.531169550624043 74-75 19.127806563039723 31.333789552579265 29.279336526868054 20.259067357512954 76-77 18.932803683650544 31.116484368762613 30.341725065540892 19.60898688204595 78-79 19.305763524885986 31.09139226935228 28.883287521242746 20.719556684518988 80-81 19.397233668493183 31.062165064188736 28.945297415668275 20.59530385164981 82-83 20.496301365399027 30.66070322938052 28.893668328608207 19.949327076612246 84-85 18.810469614069845 30.794573580257374 29.270796333288796 21.12416047238398 86-87 18.889524340230423 30.963262765647176 29.90042966286862 20.24678323125378 88-89 20.11672733316171 30.188288813793505 29.84873023993952 19.846253613105265 90-91 19.773290513689087 31.02143100383389 29.565106878027557 19.640171604449463 92-93 19.942190409929957 30.85041035358679 29.49967331390053 19.70772592258273 94-95 19.3516430517356 29.93273980995086 30.190016554349103 20.52560058396444 96-97 20.357613045348486 29.983413063597375 29.65591068472438 20.003063206329756 98-99 19.01094281580354 30.388962029276435 29.97379980969016 20.62629534522987 100-101 19.366633210370583 30.558904805975207 29.912535683095008 20.161926300559198 102-103 20.124939713492445 30.276047029993354 29.26323370309058 20.33577955342362 104-105 19.433111305186596 30.781964883924033 29.503402114264116 20.28152169662526 106-107 19.10283900569626 30.92306789890116 29.31244052817498 20.661652567227602 108-109 19.511777846210997 31.823240247858607 28.223652802421885 20.441329103508508 110-111 19.2775383512428 31.1005564290777 28.6773607501609 20.944544469518604 112-113 19.078398529660962 30.57552432967921 30.14960830063741 20.19646884002242 114-115 19.006023752845273 31.630649610500917 28.971041341812537 20.392285294841276 116-117 19.081688350835456 31.460809632821984 28.711740409134233 20.745761607208323 118-119 19.04095415773909 29.963094821054693 29.339535793135475 21.656415228070745 120-121 18.621687500814684 30.65243036093695 29.422422670333827 21.303459467914543 122-123 19.02189055536999 30.655901977237733 29.309392490244164 21.012814977148118 124-125 19.137676235912124 29.995584427857537 29.19768565362688 21.669053682603458 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13.0 1 10.5 2 10.5 3 18.5 4 21.0 5 24.0 6 26.0 7 18.5 8 26.5 9 42.5 10 39.0 11 40.0 12 45.5 13 46.5 14 53.0 15 50.5 16 52.5 17 68.5 18 81.5 19 111.0 20 147.0 21 168.0 22 226.0 23 321.5 24 391.0 25 676.5 26 1518.5 27 2327.0 28 2463.0 29 3025.0 30 4543.0 31 5788.0 32 7479.0 33 10662.5 34 14786.0 35 17498.5 36 20468.5 37 23898.5 38 23001.5 39 20887.5 40 18408.0 41 15576.0 42 13599.0 43 12017.0 44 11933.5 45 12399.5 46 10814.0 47 7927.0 48 6329.0 49 5513.5 50 4940.5 51 3768.0 52 2743.5 53 2286.0 54 1959.0 55 1897.5 56 1909.5 57 1788.5 58 1557.0 59 1506.0 60 1438.5 61 1218.5 62 1105.5 63 879.5 64 562.5 65 408.5 66 317.0 67 232.0 68 175.0 69 123.5 70 79.5 71 62.5 72 69.0 73 55.5 74 30.0 75 30.0 76 33.0 77 25.5 78 18.5 79 15.0 80 9.5 81 6.5 82 7.0 83 7.0 84 4.0 85 2.0 86 2.0 87 1.5 88 1.5 89 2.0 90 1.0 91 1.0 92 1.0 93 0.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.51741779906801E-4 2 0.0 3 0.0 4 3.258708899534005E-4 5 6.51741779906801E-4 6 6.51741779906801E-4 7 6.51741779906801E-4 8 9.776126698602015E-4 9 0.001303483559813602 10-11 0.0016293544497670022 12-13 0.0016293544497670022 14-15 0.001955225339720403 16-17 0.002118160784697103 18-19 0.001955225339720403 20-21 0.0022810962296738032 22-23 0.08863688206732494 24-25 0.0016293544497670022 26-27 0.0016293544497670022 28-29 0.001303483559813602 30-31 8.146772248835011E-4 32-33 0.001303483559813602 34-35 8.146772248835011E-4 36-37 8.146772248835011E-4 38-39 6.51741779906801E-4 40-41 0.0011405481148369016 42-43 0.0011405481148369016 44-45 8.146772248835011E-4 46-47 0.001303483559813602 48-49 1.6293544497670025E-4 50-51 0.0011405481148369016 52-53 0.0011405481148369016 54-55 6.51741779906801E-4 56-57 4.888063349301007E-4 58-59 4.888063349301007E-4 60-61 6.51741779906801E-4 62-63 4.888063349301007E-4 64-65 6.51741779906801E-4 66-67 4.888063349301007E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 1.6293544497670025E-4 78-79 1.6293544497670025E-4 80-81 4.888063349301007E-4 82-83 0.0 84-85 3.258708899534005E-4 86-87 4.888063349301007E-4 88-89 3.258708899534005E-4 90-91 4.888063349301007E-4 92-93 1.6293544497670025E-4 94-95 6.51741779906801E-4 96-97 6.51741779906801E-4 98-99 6.51741779906801E-4 100-101 6.51741779906801E-4 102-103 6.51741779906801E-4 104-105 6.51741779906801E-4 106-107 6.51741779906801E-4 108-109 4.888063349301007E-4 110-111 8.146772248835011E-4 112-113 6.51741779906801E-4 114-115 4.888063349301007E-4 116-117 8.146772248835011E-4 118-119 8.146772248835011E-4 120-121 6.51741779906801E-4 122-123 8.146772248835011E-4 124-125 4.888063349301007E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 306870.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.84931730048555 #Duplication Level Percentage of deduplicated Percentage of total 1 64.61543050604782 14.118030436341122 2 10.040418201613745 4.387525662332584 3 5.841996152067891 3.8293088278424086 4 4.083580664886874 3.568937986769642 5 2.705484048979105 2.955648971877342 6 1.8508851735298064 2.42643464659302 7 1.40046831421796 2.1419493596637014 8 1.0559441602410178 1.84573272069606 9 0.8516159823412728 1.674650503470525 >10 6.117913764560247 27.219995437807544 >50 0.8471416426792345 12.739596571838238 >100 0.578681262956942 21.665851989441784 >500 0.010440125878089158 1.4263368853260339 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 681 0.2219180760582657 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 672 0.2189852380486851 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 670 0.21833349626877832 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 665 0.2167041418190113 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 613 0.1997588555414345 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 573 0.18672401994329846 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 503 0.16391305764656044 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 493 0.16065434874702642 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 459 0.14957473848861083 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 451 0.14696777136898362 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 444 0.1446866751393098 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 444 0.1446866751393098 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 444 0.1446866751393098 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 439 0.1430573206895428 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 437 0.142405578909636 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 434 0.1414279662397758 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 423 0.13784338645028837 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 407 0.132629452211034 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 402 0.13100009776126698 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 377 0.12285332551243197 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 376 0.12252745462247858 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 374 0.12187571284257177 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 373 0.12154984195261838 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 369 0.12024635839280479 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 368 0.11992048750285138 No Hit GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC 363 0.11829113305308436 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 352 0.11470655326359697 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 350 0.11405481148369016 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 348 0.11340306970378336 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 336 0.10949261902434256 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 336 0.10949261902434256 No Hit GCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTT 333 0.10851500635448234 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 328 0.10688565190471536 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 323 0.10525629745494836 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 323 0.10525629745494836 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 322 0.10493042656499496 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 319 0.10395281389513475 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 319 0.10395281389513475 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 317 0.10330107211522793 No Hit ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT 313 0.10199758855541435 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 312 0.10167171766546095 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 307 0.10004236321569394 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.2587088995340044E-4 0.0 16-17 0.0 0.0 0.0 3.2587088995340044E-4 0.0 18-19 0.0 0.0 0.0 6.517417799068009E-4 0.0 20-21 0.0 0.0 0.0 6.517417799068009E-4 0.0 22-23 0.0 0.0 0.0 8.146772248835011E-4 0.0 24-25 0.0 0.0 0.0 9.776126698602015E-4 0.0 26-27 0.0 0.0 0.0 9.776126698602015E-4 0.0 28-29 0.0 0.0 0.0 0.0022810962296738032 0.0 30-31 0.0 0.0 0.0 0.005050998794277707 0.0 32-33 0.0 0.0 0.0 0.009450255808648615 0.0 34-35 0.0 0.0 0.0 0.016945286277576823 0.0 36-37 0.0 0.0 0.0 0.029491315540782742 0.0 38-39 0.0 0.0 0.0 0.05376869684231107 0.0 40-41 0.0 0.0 0.0 0.08765926939746473 0.0 42-43 0.0 0.0 0.0 0.12269039006745527 0.0 44-45 0.0 0.0 0.0 0.16375012220158375 0.0 46-47 0.0 0.0 0.0 0.21002378857496662 0.0 48-49 0.0 0.0 0.0 0.2602079056277903 0.0 50-51 0.0 0.0 0.0 0.3182129240394955 0.0 52-53 0.0 0.0 0.0 0.38550526281487274 0.0 54-55 0.0 0.0 0.0 0.4699058233128035 0.0 56-57 0.0 0.0 0.0 0.557076286375338 0.0 58-59 0.0 0.0 0.0 0.6569557141460554 0.0 60-61 0.0 0.0 0.0 0.7788314269886272 0.0 62-63 0.0 0.0 0.0 0.8933750448072474 0.0 64-65 0.0 0.0 0.0 1.0390393326164173 0.0 66-67 0.0 0.0 0.0 1.1871476521002378 0.0 68-69 0.0 0.0 0.0 1.3750122201583732 0.0 70-71 0.0 0.0 0.0 1.5993743278912893 0.0 72-73 0.0 0.0 0.0 1.82846156352853 0.0 74-75 0.0 0.0 0.0 2.07042069931893 0.0 76-77 0.0 0.0 0.0 2.325577606152442 0.0 78-79 0.0 0.0 0.0 2.624564147684687 0.0 80-81 0.0 0.0 0.0 2.9815557076286376 0.0 82-83 0.0 0.0 0.0 3.323557206634731 0.0 84-85 0.0 0.0 0.0 3.7030338579854662 0.0 86-87 0.0 0.0 0.0 4.106462019747776 0.0 88-89 0.0 0.0 0.0 4.548342946524587 0.0 90-91 0.0 0.0 0.0 5.0327500244403165 0.0 92-93 0.0 0.0 0.0 5.5513735458011535 0.0 94-95 0.0 0.0 0.0 6.111219734741096 0.0 96-97 0.0 0.0 0.0 6.723694072408511 0.0 98-99 0.0 0.0 0.0 7.366637338286571 0.0 100-101 0.0 0.0 0.0 8.076872942940007 0.0 102-103 0.0 0.0 0.0 8.858800143383192 0.0 104-105 0.0 0.0 0.0 9.618079316974615 0.0 106-107 0.0 0.0 0.0 10.443836152116532 0.0 108-109 0.0 0.0 0.0 11.246130283181802 0.0 110-111 0.0 0.0 0.0 12.094046338840553 0.0 112-113 0.0 0.0 0.0 13.008928862384723 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGAGTA 20 8.4801466E-4 89.23802 2 GTTACCA 35 9.147829E-5 67.990875 9 CGTTTTC 20 1.7525992E-4 59.62804 20-21 GCGGAGT 30 0.004235848 59.492012 6 GGTATCA 490 0.0 53.421406 1 CATTCCG 115 5.456968E-12 51.73219 9 CCCGGGT 35 0.007794973 50.993156 3 GACATAT 170 0.0 48.993427 1 GTGGTAC 50 5.3299824E-4 47.59361 1 GGGTATG 90 9.703217E-8 46.27157 7 TAGGCAT 130 1.8189894E-11 45.76309 5 CCTTTAG 120 4.1654857E-10 44.61901 1 GGCACAT 55 8.524253E-4 43.26692 5 TTCGATA 55 8.524253E-4 43.26692 4 GTACATG 1980 0.0 42.665993 1 AGGCTTA 170 0.0 41.994366 6 CATGGGT 200 0.0 41.64441 4 ATGGGAT 680 0.0 41.119484 5 GTATAGG 275 0.0 41.103573 1 TTAGGCA 145 5.820766E-11 41.028976 4 >>END_MODULE