FastQCFastQC Report
Thu 26 May 2016
SRR936193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936193_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306870
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10960.3571544953889269No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG7780.2535275523837456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.2307165900870075No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA7060.23006484830710075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6880.22419917228793954No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC6530.2127936911395705No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT6100.19878124287157428No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG5860.19096034151269267No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA5400.17597028057483624No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT5250.17108221722553524No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT5030.16391305764656044No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC4890.15935086518721284No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT4570.14892299670870401No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT4350.1417538371297292No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA4340.1414279662397758No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT4250.1384951282301952No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG4210.1371916446703816No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT4190.1365399028904748No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA4080.13295532310098737No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC4070.132629452211034No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC4050.1319777104311272No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC4040.1316518395411738No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG4020.13100009776126698No Hit
ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT3890.1267637761918728No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC3880.12643790530191937No Hit
GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC3860.12578616352201258No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC3810.12415680907224558No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC3710.12089810017271156No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT3610.11763939127317757No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT3600.11731352038322418No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG3550.11568416593345715No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG3460.11275132792387657No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG3350.10916674813438916No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA3310.10786326457457557No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT3300.10753739368462216No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA3250.10590803923485514No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT3220.10493042656499496No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG3210.10460455567504155No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC3110.10134584677550754No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC3100.10101997588555414No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT3090.10069410499560073No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTCG208.479268E-489.240349
GGTATCA8150.083.340211
GCAGTAT250.00205628971.392278
TCGGCGC250.00205628971.392279
GTATGGT401.7580652E-459.580941
GTGGTAC603.1160926E-759.5809361
AGAGCGC300.00422448859.5323645
GTATGCG150.004229230359.522655110-111
GTGCGAG150.004236060759.4984138-39
CTCCCGC701.3384124E-859.4935657
AGCGTTA150.00423742859.4935688-89
AGTTGCG150.00423742859.4935624-25
GAGCGCT300.004235411559.493566
GCGCTTA300.004235411559.493568
AGGCTTA1700.055.9939466
ATGGGAC1300.054.952955
TGGTACT655.447673E-754.952952
GGTATAG350.00774907451.0693781
ATAGACC350.007774104451.0277443
GAGAGCG350.007774104451.0277444