##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936193_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 306870 Sequences flagged as poor quality 0 Sequence length 125 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.445485058819695 31.0 31.0 34.0 30.0 34.0 2 31.43789552579268 31.0 31.0 34.0 30.0 34.0 3 31.740251572327043 31.0 31.0 34.0 30.0 34.0 4 35.57888030762212 37.0 35.0 37.0 33.0 37.0 5 35.217385211979014 37.0 35.0 37.0 33.0 37.0 6 35.40986085312999 37.0 35.0 37.0 33.0 37.0 7 35.244083813992894 37.0 35.0 37.0 32.0 37.0 8 35.31938605924333 37.0 35.0 37.0 32.0 37.0 9 36.97076938117118 39.0 37.0 39.0 33.0 39.0 10-11 36.85548603643237 39.0 37.0 39.0 32.0 39.0 12-13 36.85783067748558 39.0 37.0 39.0 32.0 39.0 14-15 37.96208818066282 40.0 37.0 41.0 33.0 41.0 16-17 37.85569948186529 40.0 37.0 41.0 32.0 41.0 18-19 38.029303939779055 40.0 37.0 41.0 33.0 41.0 20-21 38.090052791084176 40.0 37.0 41.0 33.0 41.0 22-23 38.02231563854401 40.0 37.0 41.0 33.0 41.0 24-25 37.985163098380426 40.0 37.0 41.0 33.0 41.0 26-27 37.844639423860265 40.0 37.0 41.0 32.0 41.0 28-29 37.706242056897054 40.0 37.0 41.0 32.0 41.0 30-31 37.553778472969015 40.0 36.5 41.0 32.0 41.0 32-33 37.34463616515137 39.5 36.0 41.0 32.0 41.0 34-35 37.20288721608499 39.0 36.0 41.0 31.0 41.0 36-37 37.007887704891324 39.0 36.0 41.0 31.0 41.0 38-39 36.97853488447877 39.0 35.5 41.0 30.5 41.0 40-41 37.030745918467105 40.0 36.0 41.0 31.0 41.0 42-43 36.93170397888357 40.0 36.0 41.0 31.0 41.0 44-45 36.73174960080816 40.0 36.0 41.0 30.0 41.0 46-47 36.39429888878026 40.0 36.0 41.0 30.0 41.0 48-49 36.08834196891192 40.0 36.0 41.0 29.0 41.0 50-51 35.937701632613155 40.0 36.0 41.0 28.0 41.0 52-53 35.86943331052237 40.0 36.0 41.0 28.0 41.0 54-55 35.71193013328119 39.0 35.5 41.0 27.5 41.0 56-57 35.56449962524847 39.0 35.0 41.0 27.0 41.0 58-59 35.4021311956203 39.0 35.0 41.0 26.5 41.0 60-61 35.253032228631014 39.0 35.0 41.0 26.0 41.0 62-63 35.07962981066902 39.0 35.0 40.0 26.0 41.0 64-65 34.86267311891029 38.5 34.5 40.0 26.0 41.0 66-67 34.600733209502394 38.0 34.0 40.0 26.0 41.0 68-69 34.302395151041154 37.0 34.0 40.0 26.0 41.0 70-71 33.97852184964317 37.0 34.0 39.0 26.0 41.0 72-73 33.69941180304363 36.0 34.0 39.0 26.0 41.0 74-75 33.458886499169026 36.0 34.0 39.0 26.0 40.0 76-77 33.12936585524815 36.0 33.5 37.5 26.0 40.0 78-79 32.77968195001141 35.0 33.0 37.0 26.0 39.0 80-81 32.46672858213576 35.0 33.0 37.0 25.5 39.0 82-83 32.160680418418224 35.0 33.0 36.0 25.0 38.5 84-85 31.84195098901815 35.0 32.5 36.0 24.5 37.0 86-87 31.662801837911818 35.0 32.0 36.0 25.0 37.0 88-89 31.48295858180989 35.0 32.0 35.0 24.0 36.5 90-91 31.35294098478183 35.0 32.0 35.0 24.0 36.0 92-93 31.22560530517809 35.0 32.0 35.0 24.0 36.0 94-95 31.082518656108448 35.0 32.0 35.0 23.0 36.0 96-97 31.03277609411151 35.0 32.0 35.0 23.0 35.5 98-99 30.967882165086195 35.0 32.0 35.0 23.0 35.0 100-101 30.931154886433994 35.0 32.0 35.0 23.0 35.0 102-103 29.928342620653698 33.5 30.0 35.0 19.5 35.0 104-105 30.673931958158178 34.0 31.5 35.0 20.0 35.0 106-107 30.852833447388143 35.0 32.0 35.0 20.0 35.0 108-109 30.832603382539837 35.0 32.0 35.0 20.0 35.0 110-111 30.808749633395248 35.0 32.0 35.0 20.0 35.0 112-113 30.73695050021182 35.0 32.0 35.0 19.0 35.0 114-115 30.750465995372636 35.0 32.0 35.0 19.5 35.0 116-117 30.664670707465703 35.0 32.0 35.0 19.0 35.0 118-119 30.545341675628116 35.0 32.0 35.0 18.5 35.0 120-121 30.367611692247532 35.0 32.0 35.0 11.0 35.0 122-123 30.182624564147687 35.0 32.0 35.0 2.0 35.0 124-125 28.103757291361163 33.0 28.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18175257839913783 1101 2 0.6219214832730096 1101 3 0.23864990012902254 1101 4 0.23172756962609498 1101 5 0.37600224595533405 1101 6 0.2874121511644887 1101 7 0.4596060929675261 1101 8 0.3855499681176582 1101 9 0.28294855395746765 1101 10-11 0.2941650191584202 1101 12-13 0.5685540318806517 1101 14-15 0.6928186645399634 1101 16-17 0.6711098482972631 1101 18-19 0.6364545763677967 1101 20-21 0.5153116875231518 1101 22-23 0.484941354941121 1101 24-25 0.5606035210664686 1101 26-27 0.5696692963637773 1101 28-29 0.6202266489712329 1101 30-31 0.4493857781442543 1101 32-33 0.5899210124309064 1101 34-35 0.46699635641890325 1101 36-37 0.5071271424984545 1101 38-39 0.3225078372965484 1101 40-41 0.10509320024853253 1101 42-43 0.19637315800649446 1101 44-45 0.14888315603072755 1101 46-47 0.40208931953160487 1101 48-49 0.49392721860613875 1101 50-51 0.4595514747555427 1101 52-53 0.5097468252307991 1101 54-55 0.45062501994100757 1101 56-57 0.21896757181624338 1101 58-59 0.576304452028026 1101 60-61 0.7147558667362901 1101 62-63 0.6436466048228056 1101 64-65 0.5572358642160538 1101 66-67 0.5731380565952406 1101 68-69 0.5364865179830716 1101 70-71 0.8100850528879349 1101 72-73 0.6696650843271073 1101 74-75 0.7621328594996442 1101 76-77 0.4134473376703838 1101 78-79 0.5396914796052599 1101 80-81 0.7186782847519453 1101 82-83 0.6301195582209544 1101 84-85 0.509971221146742 1101 86-87 0.5411412591714324 1101 88-89 0.5093839090108503 1101 90-91 0.6855498088955443 1101 92-93 0.7008502870762143 1101 94-95 0.6970979461477853 1101 96-97 0.6052440325242223 1101 98-99 0.3550381212813427 1101 100-101 0.5452096134327107 1101 102-103 0.528021036109589 1101 104-105 0.5468839674909667 1101 106-107 0.7806453604651047 1101 108-109 0.5030191470527612 1101 110-111 0.5649732877010152 1101 112-113 0.6122842355335436 1101 114-115 0.4916856133929137 1101 116-117 0.3083727123628215 1101 118-119 0.33138527543579954 1101 120-121 0.2763460009078642 1101 122-123 0.15065285521406935 1101 124-125 0.2778118646469707 1102 1 0.2884474514703186 1102 2 0.5201627309916077 1102 3 0.3836142273233172 1102 4 0.21680306323817433 1102 5 0.529569526526096 1102 6 0.23745363117112817 1102 7 0.4075818686436463 1102 8 0.21298484450723976 1102 9 0.08409340214064542 1102 10-11 0.2581230413917055 1102 12-13 0.06727229967557236 1102 14-15 0.40680157477723355 1102 16-17 0.47646491715407535 1102 18-19 0.369999457734977 1102 20-21 0.19343098402223546 1102 22-23 0.19122142794593344 1102 24-25 0.2556964362813332 1102 26-27 0.2521315495577383 1102 28-29 0.3145480360626536 1102 30-31 0.07816875474953378 1102 32-33 0.21233680801743304 1102 34-35 0.109529955224275 1102 36-37 0.09696868887309762 1102 38-39 0.12076982501846345 1102 40-41 -0.15128642477220922 1102 42-43 -0.1640632116633256 1102 44-45 -0.07887526441061965 1102 46-47 -0.051610697060397115 1102 48-49 -0.461664286288503 1102 50-51 -0.5150876491867251 1102 52-53 -0.3833467536641706 1102 54-55 -0.6718737356587923 1102 56-57 -0.684769776781728 1102 58-59 -0.5225009237808393 1102 60-61 -0.5065980406803305 1102 62-63 -0.768399189104521 1102 64-65 -0.6949169481283946 1102 66-67 -0.5361368728887541 1102 68-69 -0.4925536346633592 1102 70-71 -0.6936937757166817 1102 72-73 -0.3236566346043759 1102 74-75 -0.4308030953701092 1102 76-77 -0.3761619206670943 1102 78-79 -0.35784045999984926 1102 80-81 -0.30087953837730197 1102 82-83 -0.3532262191143687 1102 84-85 -0.25776977769181997 1102 86-87 -0.38948342972852146 1102 88-89 -0.2924992832904607 1102 90-91 -0.2579959020717695 1102 92-93 -0.3044591537732977 1102 94-95 -0.540341907842592 1102 96-97 -0.5542175569896344 1102 98-99 -0.614884725906311 1102 100-101 -0.6638537680174323 1102 102-103 -0.868988255411896 1102 104-105 -0.9367231338861686 1102 106-107 -0.7483035361667199 1102 108-109 -0.7149251038183166 1102 110-111 -0.5763532429112317 1102 112-113 -0.7128236124837173 1102 114-115 -0.6246201772160092 1102 116-117 -0.7558549299136033 1102 118-119 -0.608796406993271 1102 120-121 -0.3015368595533907 1102 122-123 -0.5846092984278748 1102 124-125 -0.8257678599257865 1103 1 -0.12173567722263812 1103 2 0.6185242111072675 1103 3 0.3866607847553247 1103 4 0.14798522809590509 1103 5 0.517932263818139 1103 6 0.2603090977930975 1103 7 0.31539731515078984 1103 8 0.33135638203395246 1103 9 0.39465787433609023 1103 10-11 0.5043263687185231 1103 12-13 0.41697267263789684 1103 14-15 0.5652602765330883 1103 16-17 0.6881734198570015 1103 18-19 0.4405459104022924 1103 20-21 0.587583018552678 1103 22-23 0.5120318876534284 1103 24-25 0.5590405254570499 1103 26-27 0.573296113946121 1103 28-29 0.620829727383942 1103 30-31 0.5279466535887423 1103 32-33 0.7623221409596468 1103 34-35 0.4666958606008009 1103 36-37 0.5708969460075721 1103 38-39 0.44698614316789786 1103 40-41 0.3267255603092991 1103 42-43 0.44065539035653245 1103 44-45 0.7397372319226108 1103 46-47 0.8912422552088017 1103 48-49 0.7971108048567928 1103 50-51 0.8185187707940003 1103 52-53 0.6580227304744568 1103 54-55 0.7754484786478812 1103 56-57 0.8712709557330456 1103 58-59 0.7102129009687772 1103 60-61 0.9408163665610019 1103 62-63 0.8253589301596094 1103 64-65 0.8310814594481855 1103 66-67 0.9450851025544011 1103 68-69 0.7642602613262497 1103 70-71 0.518752270463601 1103 72-73 0.6366689740785318 1103 74-75 0.636164595196874 1103 76-77 0.7239784640288747 1103 78-79 0.6316285925082212 1103 80-81 0.731084171131144 1103 82-83 0.6448042045540703 1103 84-85 0.5582613164706913 1103 86-87 0.6997341875031751 1103 88-89 0.6227167415178201 1103 90-91 0.7541463264828963 1103 92-93 0.6284600097853499 1103 94-95 0.7148313729687068 1103 96-97 0.8391445517142166 1103 98-99 0.7438358875957611 1103 100-101 0.7857586025981647 1103 102-103 0.7413960215021511 1103 104-105 0.5455108685443726 1103 106-107 0.5245624069250958 1103 108-109 0.5528513702209921 1103 110-111 0.4836077846209328 1103 112-113 0.5398876979131337 1103 114-115 0.5251053392739493 1103 116-117 0.6257672466777748 1103 118-119 0.7472527066973811 1103 120-121 0.7041560541624001 1103 122-123 0.6030221812687273 1103 124-125 0.3925240564300765 1104 1 0.11264688734222617 1104 2 0.4003767678258541 1104 3 0.21425965622658438 1104 4 0.09961374848788296 1104 5 0.3638071240041114 1104 6 0.1593633706766866 1104 7 0.3031020279268688 1104 8 0.2737613502314815 1104 9 0.23010302550218142 1104 10-11 0.3378642061502859 1104 12-13 0.2077816741570757 1104 14-15 0.20704630681638747 1104 16-17 0.2747683848826341 1104 18-19 0.07039766579869422 1104 20-21 0.11287266313290445 1104 22-23 0.0012015175427464442 1104 24-25 -0.09894932446198368 1104 26-27 0.06763677603857587 1104 28-29 0.17612095791432125 1104 30-31 0.004263826924749026 1104 32-33 -0.0960545973251925 1104 34-35 -0.009629659841259297 1104 36-37 -0.2631980607535809 1104 38-39 -0.28216695945142334 1104 40-41 -0.18352468593032967 1104 42-43 0.009381288087794815 1104 44-45 0.10924900968925755 1104 46-47 -0.06843913575294636 1104 48-49 -0.03656058627191072 1104 50-51 -0.21727779353714993 1104 52-53 -0.2239930121675755 1104 54-55 -0.18961888249801717 1104 56-57 -0.24851210298050574 1104 58-59 -0.16759798699636974 1104 60-61 -0.3645124259466357 1104 62-63 -0.31976802932353365 1104 64-65 -0.3502844609871971 1104 66-67 -0.3669025938112682 1104 68-69 -0.3263183600657129 1104 70-71 -0.2925572235348355 1104 72-73 -0.3557414197379387 1104 74-75 -0.33034681529710497 1104 76-77 -0.2013900607036021 1104 78-79 -0.367828845597991 1104 80-81 -0.2396550485813833 1104 82-83 -0.18898613283595012 1104 84-85 -0.11197999836545591 1104 86-87 -0.18040345627572663 1104 88-89 -0.21419332676151015 1104 90-91 -0.2707510041125829 1104 92-93 -0.2869545909725666 1104 94-95 -0.33440611889286487 1104 96-97 -0.40593482926439606 1104 98-99 -0.41528708197068553 1104 100-101 -0.3643432320957487 1104 102-103 -0.4181171752725241 1104 104-105 -0.652303024378952 1104 106-107 -0.2976066720552204 1104 108-109 -0.27035483668707627 1104 110-111 -0.289701509046953 1104 112-113 -0.2647482847916649 1104 114-115 -0.29794853294854917 1104 116-117 -0.11134273479165202 1104 118-119 -0.11881797659672344 1104 120-121 -0.07832879584010399 1104 122-123 -0.30259917730625574 1104 124-125 -0.21100927356441446 1105 1 0.2054597961230442 1105 2 0.6587221058617168 1105 3 0.4790088821302483 1105 4 0.2314088240761123 1105 5 0.5402058788266899 1105 6 0.19120066970140925 1105 7 0.3633821928466503 1105 8 0.18742684126521425 1105 9 0.43423264317310384 1105 10-11 0.1427795109041483 1105 12-13 0.27514461357057485 1105 14-15 0.4019343733141625 1105 16-17 0.5571831514946552 1105 18-19 0.2529891913066109 1105 20-21 0.3363691776296207 1105 22-23 0.4086452610229543 1105 24-25 0.3305582343610567 1105 26-27 0.3221199115012965 1105 28-29 0.3918330437520794 1105 30-31 0.2030554915216385 1105 32-33 0.00843805910850648 1105 34-35 -0.029600931346237758 1105 36-37 -0.1802227987199032 1105 38-39 -0.06685227066298438 1105 40-41 0.05455754214246156 1105 42-43 0.05420022405719038 1105 44-45 0.07138659185937968 1105 46-47 -0.02287301053976165 1105 48-49 -0.2665003143860858 1105 50-51 -0.18003423882053937 1105 52-53 -0.3325300114886858 1105 54-55 -0.07536723081652497 1105 56-57 -0.23147163471430332 1105 58-59 -0.2515108576933045 1105 60-61 -0.4990561644593825 1105 62-63 -0.31093836363380234 1105 64-65 -0.18314588532444986 1105 66-67 0.008428570396503687 1105 68-69 -0.17963331378551572 1105 70-71 -0.23507953572333662 1105 72-73 -0.16457895057760652 1105 74-75 0.0742116600228826 1105 76-77 0.11924312526165437 1105 78-79 -0.02679594443394251 1105 80-81 0.07348140763042466 1105 82-83 0.04830751046707604 1105 84-85 -0.07372846259144339 1105 86-87 0.010075986706077344 1105 88-89 0.026774169471501352 1105 90-91 0.005470991814100046 1105 92-93 -0.0019008595798553074 1105 94-95 0.062326532242199306 1105 96-97 -0.054263277925663544 1105 98-99 -0.047469205726752506 1105 100-101 -0.2846692632582979 1105 102-103 -0.12069032116691858 1105 104-105 0.03927960936999497 1105 106-107 0.007035422227229304 1105 108-109 -0.43719743433911873 1105 110-111 -0.06460919702403345 1105 112-113 -0.07132355013698444 1105 114-115 -0.020014832022944518 1105 116-117 0.004939367438741016 1105 118-119 0.029767124325569938 1105 120-121 -0.11027579300865042 1105 122-123 -0.09504821941378339 1105 124-125 0.3559645479049607 1106 1 0.21555294155367477 1106 2 0.7198178042885353 1106 3 0.3431140836038544 1106 4 0.16891573929451198 1106 5 0.3858213230193428 1106 6 0.3501871752930015 1106 7 0.3775732454760714 1106 8 0.09660082611114973 1106 9 0.2137283547132185 1106 10-11 0.3548318657665206 1106 12-13 0.18690437785895853 1106 14-15 0.021029801279105698 1106 16-17 0.3646511775737338 1106 18-19 0.5145381182696127 1106 20-21 0.3750780090294228 1106 22-23 0.19079026956708844 1106 24-25 0.3880908024785157 1106 26-27 0.398107404688524 1106 28-29 0.06430371298130666 1106 30-31 -0.06320807767357195 1106 32-33 0.02762855852045476 1106 34-35 0.061858210470035146 1106 36-37 0.28065653020681935 1106 38-39 0.023471750859222595 1106 40-41 -0.01248217067620061 1106 42-43 0.10589942127717222 1106 44-45 0.003519101786558565 1106 46-47 -0.15166818484424738 1106 48-49 0.036884147169395476 1106 50-51 0.23054327924099027 1106 52-53 0.2839714412194141 1106 54-55 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2216 42-43 -0.5558071819787287 2216 44-45 -0.7287129267482868 2216 46-47 -0.7914066154277393 2216 48-49 -0.9316086276614115 2216 50-51 -0.5572722502998886 2216 52-53 -0.6277808909554494 2216 54-55 -0.8201344035623137 2216 56-57 -1.34720020349787 2216 58-59 -1.3606966566194245 2216 60-61 -1.0058095434632648 2216 62-63 -0.6653611557093413 2216 64-65 -0.7619160204846054 2216 66-67 -0.6499587652820793 2216 68-69 -0.6759488109156777 2216 70-71 -0.8788746662547169 2216 72-73 -0.8892410501887866 2216 74-75 -0.8333309246836507 2216 76-77 -0.5481934605557797 2216 78-79 -0.5911196807790731 2216 80-81 -0.5919277758541099 2216 82-83 -0.6251649946245728 2216 84-85 -0.6616658815879255 2216 86-87 -0.4830826876134928 2216 88-89 -0.42468852262256007 2216 90-91 -0.526432822294403 2216 92-93 -0.4642457957027979 2216 94-95 -0.5119560080281218 2216 96-97 -0.7528029002178336 2216 98-99 -0.9615915904703165 2216 100-101 -0.8865346585554654 2216 102-103 -0.9194752683989122 2216 104-105 -0.7466489223833861 2216 106-107 -0.8564204192836016 2216 108-109 -1.1063212077144229 2216 110-111 -1.1452539850262582 2216 112-113 -0.9612930002388183 2216 114-115 -0.9679411419655466 2216 116-117 -0.7422185662772414 2216 118-119 -1.0729661658058873 2216 120-121 -1.2355350042658237 2216 122-123 -1.1612558661458685 2216 124-125 -1.428110160482376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14.0 3 12.0 4 21.0 5 54.0 6 134.0 7 279.0 8 444.0 9 799.0 10 1324.0 11 1769.0 12 2171.0 13 2926.0 14 4229.0 15 2906.0 16 957.0 17 725.0 18 680.0 19 631.0 20 670.0 21 688.0 22 751.0 23 946.0 24 1333.0 25 1820.0 26 2478.0 27 3232.0 28 4348.0 29 5637.0 30 7072.0 31 8926.0 32 11287.0 33 14727.0 34 19668.0 35 27339.0 36 44977.0 37 90146.0 38 40714.0 39 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.9217520599715 22.527137495557245 12.34939464391106 22.201715800560194 2 17.767632231553083 22.985202058220665 38.423160522310965 20.824005187915287 3 15.042901042464374 30.564182110281585 34.95856538131906 19.434351465934977 4 10.036823410564734 19.556489718773424 42.31042460976961 28.096262260892235 5 9.365584525688348 41.65308012364841 38.41285265231058 10.568482698352659 6 24.962687783100336 44.37937889008375 18.569426793104572 12.088506533711344 7 23.18441033662463 36.17655684817675 26.029914947697723 14.609117867500895 8 24.60553328771141 35.67732264476815 21.66552611855183 18.05161794896862 9 25.725551536481245 13.013653990289049 23.036464952585785 38.22432952064392 10-11 21.468700101019973 27.613810408316226 32.51637501222016 18.401114478443642 12-13 22.86033173656597 28.59565939974582 30.795450842376248 17.748558021311954 14-15 21.555055886857627 29.233551666829605 28.0713331378108 21.14005930850197 16-17 19.108285443812434 30.442908141734513 30.16249578403849 20.286310630414558 18-19 18.293740020203995 29.22784892625542 32.88770489132206 19.59070616221853 20-21 18.566005148760063 28.94091960765145 33.81839215302897 18.674683090559522 22-23 19.189884967575846 28.771466744875678 32.81568742464236 19.222960862906117 24-25 19.06589109394858 30.197966565646688 32.69902564603904 18.037116694365693 26-27 18.257568351419167 29.35282041255255 33.8517939192492 18.537817316779094 28-29 18.56111708541076 27.35930524326262 34.79095382409489 19.288623847231726 30-31 19.105484407077917 28.506044905008636 33.58555740215727 18.802913285756183 32-33 18.618470362042558 29.006582591977057 33.96910743963242 18.405839606347964 34-35 18.97907742542025 29.03726176762435 33.326544345397636 18.657116461557763 36-37 19.559748427672957 28.40258089744843 32.57812754586632 19.459543129012285 38-39 18.496105842865056 29.675758464496365 32.80232671815427 19.02580897448431 40-41 18.244533515821033 29.025483103594357 34.16935510150878 18.56062827907583 42-43 18.642062813149213 29.05535867041427 32.85282496232026 19.44975355411626 44-45 18.817676515016952 29.019200779568273 32.58363982120771 19.57948288420706 46-47 20.032036658019265 28.70328123166773 31.61069757134104 19.653984538971958 48-49 19.013430408582945 29.899035656903738 31.184717818023017 19.9028161164903 50-51 19.04166754930413 29.286236884686268 30.99654386327973 20.675551702729873 52-53 19.50500163761062 29.661446409396135 30.772088597178747 20.061463355814496 54-55 19.97128895015651 29.905442552643713 30.344744427661023 19.77852406953875 56-57 19.671409926138114 30.430836563670642 30.160840653530236 19.73691285666101 58-59 19.057030800640682 28.999170632909742 31.32302789052354 20.620770675926032 60-61 19.968585227235696 30.24796162444356 30.37049227339977 19.412960874920973 62-63 19.424050158537735 29.397426246549806 31.42077838281731 19.75774521209514 64-65 19.51613744199385 29.449528129725223 31.460516710985974 19.573817717294958 66-67 19.485826667731207 29.69531857205938 30.355714222866016 20.463140537343392 68-69 19.682599167393093 29.247313579965294 30.88645751016318 20.18362974247843 70-71 19.961415880754217 29.07235174117356 31.689326146606618 19.27690623146561 72-73 19.86036204845779 29.15288481905724 30.339237123816666 20.64751600866831 74-75 19.364383650924257 29.637221276283736 30.55667348285498 20.441721589937025 76-77 19.03887483115787 29.749127886609784 31.62077039262618 19.591226889606165 78-79 19.156972007690555 29.286994492781957 30.50672923387754 21.049304265649948 80-81 19.17169192466431 29.737850172632076 29.86591889306914 21.224539009634483 82-83 20.175840218724897 29.519062663444885 30.120947177872026 20.18414993995819 84-85 18.64541547044504 29.26981871743317 30.437254985026186 21.6475108270956 86-87 18.38762079646234 29.45225902215763 31.35062298468893 20.8094971966911 88-89 19.76765216257831 28.814716449281857 31.00898596299706 20.408645425142772 90-91 19.22412641307146 29.58154509934271 30.891233009740375 20.30309547784545 92-93 19.604913489284534 29.260157819529148 30.56641341026071 20.568515280925606 94-95 19.258117496773856 28.14288228163249 31.171709008433595 21.427291213160053 96-97 20.26118764613392 28.279306215223183 30.719121444923296 20.7403846937196 98-99 18.89212229442167 28.641491726030253 30.911863810262457 21.55452216928562 100-101 19.031899605369066 28.784106573163143 31.057715181396624 21.12627864007117 102-103 19.898001580486692 28.191646231679794 30.355772442503685 21.554579745329825 104-105 19.166841823126195 28.3398263425539 31.08350971181467 21.409822122505243 106-107 18.903699793233912 28.457792181341517 30.56944587013656 22.069062155288012 108-109 19.551860161600164 28.900001140553687 29.337647885006117 22.21049081284003 110-111 19.181436909149564 28.47634317147168 29.992309371812542 22.34991054756621 112-113 18.983655710724005 27.728583766519467 31.347629833623934 21.940130689132594 114-115 19.143187266448617 28.85713214791493 30.037498594413837 21.962181991222625 116-117 19.093784680971726 28.573453992559024 29.728549335184617 22.604211991284636 118-119 19.128847752049147 27.19523522414327 30.361920902113514 23.313996121694068 120-121 18.384549079289762 28.059298269054977 30.42702509813251 23.12912755352275 122-123 18.777069150374935 28.16821083711093 30.337653534428537 22.7170664780856 124-125 18.657702591945043 27.638669141237948 30.155008318274625 23.548619948542388 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 2.0 2 6.5 3 8.5 4 8.0 5 10.0 6 21.0 7 36.0 8 59.5 9 82.0 10 85.5 11 91.5 12 100.5 13 104.5 14 130.5 15 183.5 16 210.5 17 230.0 18 257.5 19 297.5 20 368.5 21 453.5 22 542.5 23 652.5 24 736.0 25 1007.5 26 1839.0 27 2645.0 28 2867.0 29 3395.0 30 4813.0 31 6240.0 32 7779.0 33 10625.5 34 14516.5 35 16887.5 36 19567.0 37 23012.0 38 22566.5 39 20661.5 40 18336.5 41 15276.5 42 13272.0 43 12142.0 44 11886.5 45 12096.5 46 10589.0 47 7988.5 48 6563.5 49 5630.0 50 4987.0 51 3861.5 52 2777.5 53 2312.5 54 1940.0 55 1811.5 56 1819.5 57 1660.0 58 1457.5 59 1387.0 60 1294.0 61 1073.0 62 956.0 63 815.0 64 512.0 65 332.0 66 268.0 67 201.5 68 153.0 69 113.5 70 67.0 71 45.5 72 37.0 73 32.5 74 24.0 75 20.5 76 10.5 77 5.5 78 7.0 79 3.0 80 1.0 81 1.0 82 1.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06224133998109949 2 9.776126698602015E-4 3 3.258708899534005E-4 4 0.0 5 0.06321895265095968 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.6293544497670025E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 1.6293544497670025E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0024440316746505036 44-45 0.010916674813438916 46-47 0.011079610258415617 48-49 0.009450255808648613 50-51 0.007983836803858311 52-53 0.00668035324404471 54-55 0.006354482354091309 56-57 0.004399257014370906 58-59 0.0030957734545573046 60-61 0.002606967119627204 62-63 9.776126698602015E-4 64-65 0.001955225339720403 66-67 0.0027699025646039043 68-69 8.146772248835011E-4 70-71 0.002606967119627204 72-73 0.0016293544497670022 74-75 8.146772248835011E-4 76-77 1.6293544497670025E-4 78-79 0.0 80-81 0.0011405481148369016 82-83 1.6293544497670025E-4 84-85 3.258708899534005E-4 86-87 1.6293544497670025E-4 88-89 8.146772248835011E-4 90-91 9.776126698602015E-4 92-93 1.6293544497670025E-4 94-95 6.51741779906801E-4 96-97 8.146772248835011E-4 98-99 0.001303483559813602 100-101 3.258708899534005E-4 102-103 8.146772248835011E-4 104-105 4.888063349301007E-4 106-107 4.888063349301007E-4 108-109 4.888063349301007E-4 110-111 9.776126698602015E-4 112-113 0.011405481148369015 114-115 0.01906344706227393 116-117 0.018085834392413725 118-119 0.011405481148369015 120-121 0.004725127904324306 122-123 0.005539805129207809 124-125 0.005050998794277707 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 306870.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.18489914295956 #Duplication Level Percentage of deduplicated Percentage of total 1 55.86337896924951 10.158699123407306 2 12.176546484123001 4.428585394466713 3 7.323847752849258 3.9955029817186434 4 5.198552075120063 3.7814058070192593 5 3.43882158985019 3.1267311891028777 6 2.4693570353379686 2.6943005181347153 7 1.754354526557236 2.233193208850653 8 1.3619095405347286 1.9812950109166747 9 1.0088882517382265 1.6511877993938802 >10 7.617733495806752 28.266692736337863 >50 1.0931116049028744 13.81301528334474 >100 0.6737868253171815 21.46022745788119 >500 0.01791986237545696 2.0520089940365627 >1k 0.0017919862375456955 0.3571544953889269 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1096 0.3571544953889269 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 778 0.2535275523837456 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 708 0.2307165900870075 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 706 0.23006484830710075 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 688 0.22419917228793954 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 653 0.2127936911395705 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 610 0.19878124287157428 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 586 0.19096034151269267 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 540 0.17597028057483624 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 525 0.17108221722553524 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 503 0.16391305764656044 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 489 0.15935086518721284 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 457 0.14892299670870401 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 435 0.1417538371297292 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 434 0.1414279662397758 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 425 0.1384951282301952 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 421 0.1371916446703816 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 419 0.1365399028904748 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 408 0.13295532310098737 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 407 0.132629452211034 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 405 0.1319777104311272 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 404 0.1316518395411738 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 402 0.13100009776126698 No Hit ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT 389 0.1267637761918728 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 388 0.12643790530191937 No Hit GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC 386 0.12578616352201258 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 381 0.12415680907224558 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 371 0.12089810017271156 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 361 0.11763939127317757 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 360 0.11731352038322418 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 355 0.11568416593345715 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 346 0.11275132792387657 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 335 0.10916674813438916 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 331 0.10786326457457557 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 330 0.10753739368462216 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 325 0.10590803923485514 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 322 0.10493042656499496 No Hit CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG 321 0.10460455567504155 No Hit CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC 311 0.10134584677550754 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 310 0.10101997588555414 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 309 0.10069410499560073 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.2587088995340044E-4 0.0 16-17 0.0 0.0 0.0 3.2587088995340044E-4 0.0 18-19 0.0 0.0 0.0 6.517417799068009E-4 0.0 20-21 0.0 0.0 0.0 6.517417799068009E-4 0.0 22-23 0.0 0.0 0.0 8.146772248835011E-4 0.0 24-25 0.0 0.0 0.0 9.776126698602015E-4 0.0 26-27 0.0 0.0 0.0 9.776126698602015E-4 0.0 28-29 0.0 0.0 0.0 0.0022810962296738032 0.0 30-31 0.0 0.0 0.0 0.005050998794277707 0.0 32-33 0.0 0.0 0.0 0.009287320363671914 0.0 34-35 0.0 0.0 0.0 0.016945286277576823 0.0 36-37 0.0 0.0 0.0 0.02998012187571284 0.0 38-39 0.0 0.0 0.0 0.054420438622217876 0.0 40-41 0.0 0.0 0.0 0.08847394662234823 0.0 42-43 0.0 0.0 0.0 0.12431974451722227 0.0 44-45 0.0 0.0 0.0 0.16652002476618766 0.0 46-47 0.0 0.0 0.0 0.2127936911395705 0.0 48-49 0.0 0.0 0.0 0.2633036790823476 0.0 50-51 0.0 0.0 0.0 0.32326392283377325 0.0 52-53 0.0 0.0 0.0 0.39071919705412717 0.0 54-55 0.0 0.0 0.0 0.4764232411118715 0.0 56-57 0.0 0.0 0.0 0.5650601231791964 0.0 58-59 0.0 0.0 0.0 0.6678723889594942 0.0 60-61 0.0 0.0 0.0 0.7910515853618796 0.0 62-63 0.0 0.0 0.0 0.9070616221852902 0.0 64-65 0.0 0.0 0.0 1.055007006224134 0.0 66-67 0.0 0.0 0.0 1.204907615602698 0.0 68-69 0.0 0.0 0.0 1.3937497963306937 0.0 70-71 0.0 0.0 0.0 1.6216964838530974 0.0 72-73 0.0 0.0 0.0 1.8538794929448952 0.0 74-75 0.0 0.0 0.0 2.0997490794147358 0.0 76-77 0.0 0.0 0.0 2.357512953367876 0.0 78-79 0.0 0.0 0.0 2.65649949490012 0.0 80-81 0.0 0.0 0.0 3.0152833447388145 0.0 82-83 0.0 0.0 0.0 3.3598918108645357 0.0 84-85 0.0 0.0 0.0 3.742301300224851 0.0 86-87 0.0 0.0 0.0 4.1496399126666015 0.0 88-89 0.0 0.0 0.0 4.592987258448203 0.0 90-91 0.0 0.0 0.0 5.079838368038583 0.0 92-93 0.0 0.0 0.0 5.605631048978395 0.0 94-95 0.0 0.0 0.0 6.16824714048294 0.0 96-97 0.0 0.0 0.0 6.784794864274774 0.0 98-99 0.0 0.0 0.0 7.432952064392088 0.0 100-101 0.0 0.0 0.0 8.153941408413987 0.0 102-103 0.0 0.0 0.0 8.941897220321309 0.0 104-105 0.0 0.0 0.0 9.707856747156777 0.0 106-107 0.0 0.0 0.0 10.541597419102551 0.0 108-109 0.0 0.0 0.0 11.352852999641541 0.0 110-111 0.0 0.0 0.0 12.206145924984522 0.0 112-113 0.0 0.0 0.0 13.130315768892364 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGTCG 20 8.479268E-4 89.24034 9 GGTATCA 815 0.0 83.34021 1 GCAGTAT 25 0.002056289 71.39227 8 TCGGCGC 25 0.002056289 71.39227 9 GTATGGT 40 1.7580652E-4 59.58094 1 GTGGTAC 60 3.1160926E-7 59.580936 1 AGAGCGC 30 0.004224488 59.532364 5 GTATGCG 15 0.0042292303 59.522655 110-111 GTGCGAG 15 0.0042360607 59.49841 38-39 CTCCCGC 70 1.3384124E-8 59.493565 7 AGCGTTA 15 0.004237428 59.49356 88-89 AGTTGCG 15 0.004237428 59.49356 24-25 GAGCGCT 30 0.0042354115 59.49356 6 GCGCTTA 30 0.0042354115 59.49356 8 AGGCTTA 170 0.0 55.993946 6 ATGGGAC 130 0.0 54.95295 5 TGGTACT 65 5.447673E-7 54.95295 2 GGTATAG 35 0.007749074 51.069378 1 ATAGACC 35 0.0077741044 51.027744 3 GAGAGCG 35 0.0077741044 51.027744 4 >>END_MODULE