FastQCFastQC Report
Thu 26 May 2016
SRR936192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3578326
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40800.064.306361
GTACATG252100.056.449991
TACATGG257550.054.6086462
ACATGGG257200.052.739913
CATGGGA153950.046.8380134
CATGGGG100850.044.0676844
GTATCAA59300.043.6425442
ATGGGAG62050.038.4483835
ATCAACG69950.036.742744
TCAACGC70900.036.334335
ATGGGGG61200.036.2604265
CAACGCA72050.035.5892456
AACGCAG74600.034.691737
ATGGGAT72750.034.4290125
GAGTACA149900.032.2674331
AGTACAT142800.032.0386052
TATCAAC85550.030.5295223
ATGGGGA52700.027.9973285
ACGCAGA90800.027.9784478
CGCAGAG91750.027.3645279
Produced by FastQC (version 0.11.5)