FastQCFastQC Report
Thu 26 May 2016
SRR936192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3578326
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80910.22611131573814125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57830.16161188220413678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56580.15811862865485146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70000.080.088521
GTATCAA106650.052.248972
GTACATG226450.050.092561
TACATGG230950.048.5652542
ACATGGG228750.047.807793
CATGGGA137850.042.8837854
ATCAACG150500.038.171694
CATGGGG94250.037.9615334
TCAACGC152200.037.7453355
CAACGCA154100.037.333726
AACGCAG157050.036.6703347
TATCAAC164500.035.3573153
ATGGGAG54000.034.8372545
ATGGGAT62500.032.0044145
AGTACAT139850.030.7778742
ACGCAGA185900.030.7554058
CGCAGAG188100.030.3008049
GAGTACA147950.030.2415161
ATGGGGG57850.029.431565
CAGAGTA211800.026.9102069
Produced by FastQC (version 0.11.5)