FastQCFastQC Report
Thu 26 May 2016
SRR936191_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936191_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82594
Sequences flagged as poor quality0
Sequence length125
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10471.2676465602828293No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5930.7179698283168269No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5090.6162675254861129No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4860.588420466377703No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3050.3692762186115214No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT2820.34142915950311165No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2770.3353754510012834No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2530.3063176501925079No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2460.29784245828994843No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2380.28815652468702324No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC2370.2869457829866576No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180.2639416906797104No Hit
GAACAAAAAACACCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA1800.2179335060658159No Hit
ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.17192532145192144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.17071457975155582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.1682930963508245No Hit
GAACAAAAAAACACAAAGAACATGGGACTCCCGACCCGGGGAGGTAGTGA1380.16708235465045887No Hit
GAACAAAAAAACACAAAAAACATGGGACTCCCGACCCGGGGAGGTAGTGA1360.1646608712497276No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1350.16345012954936194No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG1240.15013197084533986No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT1170.14165677894278034No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1110.1343923287405865No Hit
GAACAAAAAAACACAGAGAACATGGGACTCCCGACCCGGGGAGGTAGTGA1090.1319708453398552No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA1070.1295493619391239No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1030.12470639513766134No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA1450.094.36251
CTAGACT208.459506E-489.23414
TACAAGG401.880835E-889.23412
GGAGATA304.2445266E-579.31928
CAGTTGA250.002051519771.3872761
GTAGCCC250.002051519771.3872763
AGGCATC250.002051519771.3872766
CAACTCA250.002051519771.3872766
AAGGCAT250.002051519771.3872765
GAACAAA5600.069.05021
GTACATA701.72804E-1067.9878851
TACATAA453.960382E-666.0993352
AGAACAA2850.060.5330732
TACATGA401.7638673E-459.4894032
CCCTCGG150.004233123759.489448-49
CCTAGAC300.00422563659.48943
GAGAACA4400.055.4333081
TCCTAGA453.1564655E-452.8794672
ACATGGA1600.052.0532263
TAGACTG350.007776268350.9909175
Produced by FastQC (version 0.11.5)