##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936191_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82594 Sequences flagged as poor quality 0 Sequence length 125 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.476414751676877 29.0 12.0 31.0 2.0 34.0 2 22.741869869482045 28.0 12.0 31.0 2.0 34.0 3 23.499830496161948 28.0 16.0 31.0 2.0 34.0 4 25.227098820737584 32.0 12.0 37.0 2.0 37.0 5 27.61904012397995 33.0 26.0 37.0 2.0 37.0 6 25.42131389689324 32.0 12.0 37.0 2.0 37.0 7 25.92579364118459 33.0 13.0 37.0 2.0 37.0 8 24.940310434171973 32.0 8.0 37.0 2.0 37.0 9 25.180218902099426 33.0 8.0 39.0 2.0 39.0 10-11 26.255817613870256 33.0 10.5 39.0 2.0 39.0 12-13 26.119094607356466 33.0 10.0 39.0 2.0 39.0 14-15 26.712497275831176 33.0 9.5 40.0 2.0 41.0 16-17 26.89711722401143 33.0 10.5 40.0 2.0 41.0 18-19 26.687362278131584 33.0 9.5 40.0 2.0 41.0 20-21 26.914049446691042 33.0 12.5 40.0 2.0 41.0 22-23 27.09112647407802 32.5 14.5 40.0 2.0 41.0 24-25 27.057528391892873 32.0 16.0 40.0 2.0 41.0 26-27 27.132751773736594 32.5 17.0 40.0 2.0 41.0 28-29 26.9976693222268 32.0 15.5 40.0 2.0 41.0 30-31 26.783634404436157 32.0 9.0 40.0 2.0 41.0 32-33 26.433421313896893 32.0 2.0 40.0 2.0 41.0 34-35 26.06771072959295 32.0 2.0 40.0 2.0 41.0 36-37 25.558969174516307 31.0 2.0 39.0 2.0 41.0 38-39 24.944529868997748 30.0 2.0 39.0 2.0 41.0 40-41 24.69447538562123 30.5 2.0 39.0 2.0 41.0 42-43 24.290384289415698 30.0 2.0 39.0 2.0 41.0 44-45 24.05436835605492 30.0 2.0 39.0 2.0 41.0 46-47 23.863349637988232 30.0 2.0 39.0 2.0 41.0 48-49 23.59355401118725 30.0 2.0 39.0 2.0 41.0 50-51 23.358325059931715 30.0 2.0 39.0 2.0 41.0 52-53 23.069193888175896 30.0 2.0 39.0 2.0 41.0 54-55 22.839915732377655 30.0 2.0 38.5 2.0 41.0 56-57 22.59109015182701 30.0 2.0 38.0 2.0 41.0 58-59 22.317032714240746 30.0 2.0 38.0 2.0 40.0 60-61 22.006350340218418 30.0 2.0 38.0 2.0 40.0 62-63 21.695450032690026 30.0 2.0 37.5 2.0 40.0 64-65 21.336676998329175 29.0 2.0 37.0 2.0 40.0 66-67 21.08132552001356 29.0 2.0 36.5 2.0 40.0 68-69 20.87861709082984 29.0 2.0 36.0 2.0 39.5 70-71 20.54179480349662 28.5 2.0 36.0 2.0 39.0 72-73 20.237729132866797 28.0 2.0 35.0 2.0 39.0 74-75 19.890119136983316 27.5 2.0 35.0 2.0 39.0 76-77 19.54301765261399 26.5 2.0 35.0 2.0 37.5 78-79 19.29741869869482 26.0 2.0 35.0 2.0 37.0 80-81 19.061705450759135 26.0 2.0 35.0 2.0 37.0 82-83 18.805688064508317 26.0 2.0 34.0 2.0 36.0 84-85 18.760569775044193 26.0 2.0 35.0 2.0 36.0 86-87 18.581834031527713 25.0 2.0 34.5 2.0 36.0 88-89 18.359638714676613 25.0 2.0 34.0 2.0 35.0 90-91 18.132642806983558 24.0 2.0 34.0 2.0 35.0 92-93 18.037690389132383 24.0 2.0 34.0 2.0 35.0 94-95 17.93943870014771 23.5 2.0 34.0 2.0 35.0 96-97 17.79684965009565 22.0 2.0 34.0 2.0 35.0 98-99 17.624585320967626 20.0 2.0 34.0 2.0 35.0 100-101 17.469380342397752 19.0 2.0 34.0 2.0 35.0 102-103 16.796456159043032 17.0 2.0 33.5 2.0 35.0 104-105 17.1602719325859 17.0 2.0 34.0 2.0 35.0 106-107 17.197235876698066 17.0 2.0 34.0 2.0 35.0 108-109 17.1377521369591 12.5 2.0 34.0 2.0 35.0 110-111 17.006998087028116 8.5 2.0 34.0 2.0 35.0 112-113 16.89166888635978 4.5 2.0 34.0 2.0 35.0 114-115 16.702666053224206 2.0 2.0 34.0 2.0 35.0 116-117 16.567389883042352 2.0 2.0 34.0 2.0 35.0 118-119 16.42437101968666 2.0 2.0 34.0 2.0 35.0 120-121 16.210971741288716 2.0 2.0 34.0 2.0 35.0 122-123 15.913359323921835 2.0 2.0 34.0 2.0 35.0 124-125 14.607586507494492 2.0 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2585803855289939 1101 2 -0.3619638520241608 1101 3 0.1779959494019181 1101 4 -0.48966072837861674 1101 5 -1.2173209045494815 1101 6 -0.337829585851285 1101 7 -1.0480026315234205 1101 8 -0.2938746276281883 1101 9 -1.6453056102909684 1101 10-11 -0.9769138872768615 1101 12-13 -0.9612355484930077 1101 14-15 -0.2318185490095459 1101 16-17 -1.2050107531783247 1101 18-19 -1.1434486937617478 1101 20-21 -1.5558163931635747 1101 22-23 -1.6388892767863226 1101 24-25 -0.9552592779618116 1101 26-27 -0.19919528009224052 1101 28-29 -0.32648482959679725 1101 30-31 -0.5553916150331588 1101 32-33 -0.13044304321115874 1101 34-35 -0.2928306028998229 1101 36-37 -0.6652744679807441 1101 38-39 -0.6111797892421862 1101 40-41 -0.7701311606938326 1101 42-43 -0.7031671716144352 1101 44-45 -0.818229018811877 1101 46-47 -1.0555144259017375 1101 48-49 -0.8872390237029677 1101 50-51 -1.0731550199947435 1101 52-53 -1.005927687101817 1101 54-55 -0.8837668173056272 1101 56-57 -1.1434799661695116 1101 58-59 -1.008222559041947 1101 60-61 -0.8712291290581042 1101 62-63 -0.6042146568757616 1101 64-65 -0.6798664752354178 1101 66-67 -0.7623737719921735 1101 68-69 -1.044099503732479 1101 70-71 -1.0418105592701252 1101 72-73 -1.1120360988653566 1101 74-75 -0.809770670437981 1101 76-77 -0.4274619321758877 1101 78-79 -0.4564246074336964 1101 80-81 -0.42593884082036126 1101 82-83 -0.2824809218092099 1101 84-85 -0.25672409069309055 1101 86-87 -0.6147684564049243 1101 88-89 -0.8785200773562636 1101 90-91 -0.29235141540685206 1101 92-93 -0.33749480211491445 1101 94-95 -0.5261761795021584 1101 96-97 -0.573662789282821 1101 98-99 -0.6973271159940246 1101 100-101 -0.5627395145675997 1101 102-103 -0.6202200183958197 1101 104-105 -0.5105602772720665 1101 106-107 -0.7585729544931716 1101 108-109 -1.079663236051502 1101 110-111 -0.8357107423438599 1101 112-113 -0.9434320222719261 1101 114-115 -0.8746858940518933 1101 116-117 -0.7114619356779439 1101 118-119 -0.633972764544211 1101 120-121 -1.0351695443918807 1101 122-123 -1.003349286976185 1101 124-125 -1.2630899395100599 1102 1 0.8854999880583527 1102 2 0.24472088148984383 1102 3 0.4291251273603649 1102 4 0.9824259924885901 1102 5 1.061089212885026 1102 6 0.6112219046636191 1102 7 -0.09858980406181317 1102 8 -0.21998122202746728 1102 9 -0.2922794133623299 1102 10-11 0.4127880097421084 1102 12-13 0.4016370802332787 1102 14-15 1.240358506094342 1102 16-17 0.7901111980411883 1102 18-19 -0.0037919638611185746 1102 20-21 -0.09637646543096423 1102 22-23 -0.3648152027122471 1102 24-25 0.739591670547675 1102 26-27 0.8923133016602449 1102 28-29 0.5403625055432215 1102 30-31 0.4838134436840953 1102 32-33 0.6671179323985967 1102 34-35 0.6468261270008107 1102 36-37 0.41430999453054795 1102 38-39 -0.13204699791427288 1102 40-41 -0.7205376647588722 1102 42-43 -0.7888040279829944 1102 44-45 -0.8738749086041082 1102 46-47 -1.0153156905810512 1102 48-49 -0.6054865395114604 1102 50-51 -0.8031459865710779 1102 52-53 -1.1500107945995595 1102 54-55 -1.1020143331141163 1102 56-57 -0.972748258852441 1102 58-59 -0.662513435284044 1102 60-61 -0.656504648479963 1102 62-63 -0.7858768068305935 1102 64-65 -0.6623416333203309 1102 66-67 -0.970503853292989 1102 68-69 -0.4824915543196546 1102 70-71 -0.47812492241375537 1102 72-73 -0.06352661377050595 1102 74-75 -0.025488827619550847 1102 76-77 -0.5004519954098541 1102 78-79 -0.22083291818347206 1102 80-81 0.17207380597276867 1102 82-83 0.27316496798302126 1102 84-85 -0.10857594254494174 1102 86-87 -0.037803686757229116 1102 88-89 -0.12477120653421991 1102 90-91 -0.0199936918296153 1102 92-93 -0.255832652160084 1102 94-95 -0.2529151135581671 1102 96-97 0.14440405805232004 1102 98-99 0.2993305172489755 1102 100-101 0.1322921024152386 1102 102-103 0.42467609723200184 1102 104-105 0.22756077060507707 1102 106-107 0.025437885615229305 1102 108-109 -0.2660227663134691 1102 110-111 -0.6437504894079993 1102 112-113 -0.5979939012240507 1102 114-115 -0.32689908285044567 1102 116-117 -0.38698135753882923 1102 118-119 -0.81400889823888 1102 120-121 -0.6525137178336138 1102 122-123 -0.4131957187738351 1102 124-125 -0.15179815992559753 1103 1 2.4319387344970984 1103 2 2.4427265794955417 1103 3 1.9570453552805915 1103 4 3.1072123172749144 1103 5 1.8981262499220612 1103 6 2.542845836287551 1103 7 2.026481421009411 1103 8 2.4438364417901965 1103 9 2.3800852590023425 1103 10-11 2.293129890083989 1103 12-13 2.5089020374982383 1103 14-15 2.2654297311655682 1103 16-17 1.9413932493232409 1103 18-19 2.2635869535178017 1103 20-21 2.6885238194693173 1103 22-23 2.9431620052649627 1103 24-25 3.1784805594365615 1103 26-27 3.1091224184693615 1103 28-29 3.1768297420104545 1103 30-31 3.3792550391256917 1103 32-33 3.795323060603728 1103 34-35 3.966769146943829 1103 36-37 3.3563327865533417 1103 38-39 3.0008589849917158 1103 40-41 3.5589495147283046 1103 42-43 3.6949566557776876 1103 44-45 3.478831644102442 1103 46-47 3.7037726285072665 1103 48-49 3.4523482183232943 1103 50-51 3.063805580380489 1103 52-53 3.4712142766255134 1103 54-55 3.4263332452334616 1103 56-57 2.85673892063474 1103 58-59 2.593184570413964 1103 60-61 2.4258315338562184 1103 62-63 2.9758041048503188 1103 64-65 2.596775175796477 1103 66-67 2.6692397364505993 1103 68-69 2.642579670751573 1103 70-71 2.55093490664607 1103 72-73 3.1484391981953053 1103 74-75 3.150579548448821 1103 76-77 2.9065280615701994 1103 78-79 3.001674204323649 1103 80-81 3.287600871499837 1103 82-83 3.40763790245596 1103 84-85 3.6317374477684474 1103 86-87 3.460059561106018 1103 88-89 2.9934624116993973 1103 90-91 2.809493487657562 1103 92-93 3.1722300259025964 1103 94-95 3.183267222624167 1103 96-97 3.2372815509298114 1103 98-99 3.25289177081023 1103 100-101 3.3832892534123893 1103 102-103 3.0649894875453967 1103 104-105 3.265737408781714 1103 106-107 3.466036176213521 1103 108-109 3.009048458757757 1103 110-111 3.4449959493384377 1103 112-113 3.3978750446448984 1103 114-115 3.6879157319643667 1103 116-117 4.012021491464022 1103 118-119 3.260492526262542 1103 120-121 3.4389392736193756 1103 122-123 3.463442457864339 1103 124-125 3.4491989910715546 1104 1 0.5019021044604699 1104 2 -0.30660186983291027 1104 3 0.2730404712757064 1104 4 0.4125847226473205 1104 5 0.815586567382379 1104 6 -0.23639714295542902 1104 7 -0.22821943369144293 1104 8 -0.045907147953393235 1104 9 -0.45947518055809766 1104 10-11 -0.541180244226144 1104 12-13 -0.3888391102429125 1104 14-15 0.06231617805201495 1104 16-17 0.2520159599459504 1104 18-19 -0.4175485776177297 1104 20-21 -0.19664101569551562 1104 22-23 -0.16269880059584452 1104 24-25 0.4848297657857721 1104 26-27 0.08226039160733478 1104 28-29 0.45729372247443933 1104 30-31 0.31820497807563086 1104 32-33 0.08616555144621785 1104 34-35 0.28227586245054326 1104 36-37 0.6606062908268449 1104 38-39 0.8941434782762059 1104 40-41 0.6985099542887454 1104 42-43 -0.06737545655442645 1104 44-45 -0.011705596434794074 1104 46-47 0.34711817185280935 1104 48-49 0.0027145186895936035 1104 50-51 0.11325612983103639 1104 52-53 0.07353417894541536 1104 54-55 0.029996248896466682 1104 56-57 0.1978866617824835 1104 58-59 0.20838603561542968 1104 60-61 -0.5210549130302304 1104 62-63 -0.3681519391057293 1104 64-65 -0.5541405751192734 1104 66-67 -0.433202265991401 1104 68-69 -1.0216449934730925 1104 70-71 -1.005902700191534 1104 72-73 -0.719082169326061 1104 74-75 -1.3239015260322518 1104 76-77 -1.3041556991135579 1104 78-79 -1.329827627178183 1104 80-81 -1.2578203739214082 1104 82-83 -1.2080519632339062 1104 84-85 -0.982914566883565 1104 86-87 -1.4235179724715152 1104 88-89 -0.9028135345765484 1104 90-91 -1.1112635330994607 1104 92-93 -1.2243511706785988 1104 94-95 -0.7000050606481167 1104 96-97 -0.9021567885085275 1104 98-99 -1.2646906467721877 1104 100-101 -0.588871918748783 1104 102-103 -0.958392685836781 1104 104-105 -0.8197937267494204 1104 106-107 -0.9920224318450899 1104 108-109 -0.8170809673716697 1104 110-111 -0.7575071031646132 1104 112-113 -0.6604277636579106 1104 114-115 -0.9184334743848375 1104 116-117 -1.4301030506605237 1104 118-119 -1.014537998767981 1104 120-121 -1.6231486384685354 1104 122-123 -1.659756565334682 1104 124-125 -1.2610574191848567 1105 1 1.6483053466083923 1105 2 2.0838067758948853 1105 3 1.7372417546259271 1105 4 2.142236867193084 1105 5 2.0229489449575233 1105 6 1.5393542923704757 1105 7 2.0353739469232153 1105 8 2.5746602279331334 1105 9 3.081558254092357 1105 10-11 2.784892028654639 1105 12-13 2.813457411202549 1105 14-15 2.5899250939588043 1105 16-17 2.9546502051333903 1105 18-19 3.6315902268402205 1105 20-21 2.8735210917006313 1105 22-23 2.804373134561196 1105 24-25 2.6774167296493268 1105 26-27 2.437545768169308 1105 28-29 2.8333491091681218 1105 30-31 3.0328284161033245 1105 32-33 3.2472597763702282 1105 34-35 3.553130303517751 1105 36-37 3.7273911608032044 1105 38-39 3.8641704961755643 1105 40-41 3.770951696943257 1105 42-43 4.50717705948745 1105 44-45 4.169229898330485 1105 46-47 4.181295814541091 1105 48-49 4.30641259366427 1105 50-51 3.6568224925463397 1105 52-53 3.8846621762436264 1105 54-55 3.036835154671543 1105 56-57 3.126542521289405 1105 58-59 2.688629196709652 1105 60-61 2.5594921994317765 1105 62-63 3.0267315462032904 1105 64-65 3.107713528224192 1105 66-67 3.137297565146728 1105 68-69 2.9273587214880727 1105 70-71 2.4627734227399074 1105 72-73 2.150894190012 1105 74-75 2.1534709832551506 1105 76-77 1.9535275160164645 1105 78-79 1.2861804781916248 1105 80-81 1.4045403150350246 1105 82-83 1.4002729270058758 1105 84-85 1.3602971859026525 1105 86-87 1.5808724361742144 1105 88-89 1.789097981803053 1105 90-91 1.7105027620710942 1105 92-93 1.0989348813308553 1105 94-95 1.3254930818713042 1105 96-97 1.3388091013935295 1105 98-99 1.6755322508975183 1105 100-101 1.3326073112410874 1105 102-103 1.204896743410096 1105 104-105 0.9094362631188666 1105 106-107 0.8869036066554159 1105 108-109 1.236026091054537 1105 110-111 0.9098350109860114 1105 112-113 0.8931014494457692 1105 114-115 1.0503270794820985 1105 116-117 0.6800793201601465 1105 118-119 0.6624292420290452 1105 120-121 0.9150985753105907 1105 122-123 0.6154882843782516 1105 124-125 0.2974532191130166 1106 1 0.16982190395921393 1106 2 0.6048434100334248 1106 3 -0.4817632045805986 1106 4 -0.17931168503855943 1106 5 0.5507578288694326 1106 6 -1.2756619716939426 1106 7 -0.34893789651516727 1106 8 -0.7664585263995072 1106 9 -1.1812796918362949 1106 10-11 0.2111171659660016 1106 12-13 0.21725946953988284 1106 14-15 -0.5874331953815712 1106 16-17 -0.26051536837485045 1106 18-19 -0.21579419165281877 1106 20-21 0.9894492098683934 1106 22-23 0.6217623522863605 1106 24-25 -3.054226207480731 1106 26-27 0.10941160296907171 1106 28-29 0.4117632295755236 1106 30-31 -0.06232969193272453 1106 32-33 -3.8699747994309774 1106 34-35 -0.8927450230966549 1106 36-37 -0.07978635746054152 1106 38-39 0.4244275217181439 1106 40-41 -0.5308133539819302 1106 42-43 0.29206481078058033 1106 44-45 -0.10965881280906586 1106 46-47 0.20718500560385422 1106 48-49 -0.09941580712494158 1106 50-51 0.10573733283855802 1106 52-53 -0.29885512765441646 1106 54-55 -0.2676227987225843 1106 56-57 -0.3875352262709839 1106 58-59 -0.45201496689083953 1106 60-61 -0.5461175696718321 1106 62-63 -0.36627223985760793 1106 64-65 -0.18885235456789573 1106 66-67 -0.07752139715263695 1106 68-69 0.03182200736233298 1106 70-71 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2215 98-99 0.9021577719846263 2215 100-101 0.6895722833595421 2215 102-103 0.6994287124426322 2215 104-105 0.8387849719056142 2215 106-107 0.6210273682276117 2215 108-109 0.7821253818346783 2215 110-111 0.4755596604364989 2215 112-113 0.5712455672520598 2215 114-115 0.7917325258574976 2215 116-117 0.4923796828909133 2215 118-119 0.7397937593805715 2215 120-121 0.7620749166023479 2215 122-123 1.117112451992348 2215 124-125 0.608535456133211 2216 1 0.7870356700818384 2216 2 0.556735334967712 2216 3 -0.3423292538501137 2216 4 -0.733563167403009 2216 5 1.002191290572469 2216 6 -1.6955531630870588 2216 7 -0.36507580225512726 2216 8 -1.2369640585224992 2216 9 -0.9786389436243006 2216 10-11 0.03528123467435762 2216 12-13 -0.6563574997125201 2216 14-15 -1.2684039148631996 2216 16-17 -0.416392866999459 2216 18-19 0.6289090298154854 2216 20-21 0.3262974279746338 2216 22-23 0.5684277963844089 2216 24-25 0.7723829984609516 2216 26-27 1.1625933377939397 2216 28-29 0.5271737069885667 2216 30-31 0.13973033618635355 2216 32-33 0.9386626478457529 2216 34-35 1.2356246816530216 2216 36-37 1.0583027677916164 2216 38-39 1.0920722432781425 2216 40-41 0.6079176197939731 2216 42-43 0.8090279503367874 2216 44-45 0.3118522820011336 2216 46-47 -0.21405101126759263 2216 48-49 0.035525203939307914 2216 50-51 0.08538156537110808 2216 52-53 -0.550643134256287 2216 54-55 -0.6276692563300159 2216 56-57 -0.25730110438089326 2216 58-59 -0.5976534533508904 2216 60-61 -1.1801722184889982 2216 62-63 -1.238360998339175 2216 64-65 -1.4928746053167181 2216 66-67 -1.1566827150816081 2216 68-69 -1.275806820805652 2216 70-71 -1.8344934860993938 2216 72-73 -1.8762637411417806 2216 74-75 -2.167494247673755 2216 76-77 -1.8502261598181633 2216 78-79 -1.7247172903605268 2216 80-81 -1.4543941253732093 2216 82-83 -1.6198792957929484 2216 84-85 -1.801439537847564 2216 86-87 -1.5253375620959844 2216 88-89 -1.3581948741042318 2216 90-91 -1.4061725536182337 2216 92-93 -1.6789582167490629 2216 94-95 -1.570891626656632 2216 96-97 -1.4598416510714394 2216 98-99 -1.3965141078639434 2216 100-101 -1.4001378885513382 2216 102-103 -1.3681874980706166 2216 104-105 -1.2300724723940188 2216 106-107 -1.059385962623253 2216 108-109 -0.9699071384905231 2216 110-111 -0.8316457016934571 2216 112-113 -0.7930255182068855 2216 114-115 -1.3909460566535188 2216 116-117 -1.130161122669815 2216 118-119 -1.0851922767393312 2216 120-121 -0.5636248289447252 2216 122-123 -0.5277395308786232 2216 124-125 -1.0882931915425793 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 12177.0 3 1631.0 4 2003.0 5 1951.0 6 3163.0 7 2792.0 8 2323.0 9 1654.0 10 1521.0 11 1344.0 12 1124.0 13 973.0 14 928.0 15 809.0 16 772.0 17 730.0 18 616.0 19 548.0 20 540.0 21 555.0 22 535.0 23 750.0 24 962.0 25 1328.0 26 1439.0 27 1129.0 28 1170.0 29 1413.0 30 1599.0 31 1820.0 32 2311.0 33 2847.0 34 3785.0 35 4841.0 36 6689.0 37 8726.0 38 3090.0 39 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.51500108966753 20.09346925926823 8.810567353560792 14.580962297503449 2 15.52049785698719 35.38271545148558 30.740731772283702 18.35605491924353 3 16.918904520909507 47.272198949076206 21.855098433300242 13.953798096714046 4 8.15797757706371 26.894205390222048 25.849335302806498 39.09848172990774 5 7.78264764995036 59.66898321912003 22.76073322517374 9.787635905755867 6 19.462672833377727 45.582003535365764 17.164685086083733 17.790638545172772 7 22.166259050294208 51.27006804368356 14.342446182531418 12.22122672349081 8 23.008003099536282 47.89510006901311 14.334144540094195 14.76275229135641 9 23.164190669911495 34.91700265155643 11.99496325354449 29.923843424987588 10-11 18.249841087265793 45.08036443987044 18.104549444562156 18.565245028301604 12-13 21.429306535097012 43.01843387716802 18.12392166358931 17.428337924145655 14-15 20.661452216343186 41.6249954595537 17.448632417575766 20.264919906527346 16-17 19.502848390553392 42.81969475908246 19.66751623976123 18.009940610602914 18-19 19.597290245246125 40.01065497847816 21.997081988848596 18.394972787427125 20-21 24.3013682043831 35.76159341324615 20.506114541712073 19.430923840658675 22-23 24.136092344048233 37.3599539491623 20.68955070136638 17.81440300542309 24-25 24.677934908950018 36.21960964742348 21.49179097249128 17.610664471135216 26-27 24.762838790916742 35.51878825302846 21.547011496340424 18.171361459714376 28-29 21.397221297333292 37.04452583467022 23.35563156460938 18.20262130338711 30-31 21.277226883634206 38.03772716816195 23.070357052050415 17.614688896153428 32-33 21.615895001937233 38.39960286710578 21.41914471135219 18.565357419604805 34-35 20.445561037624483 38.93392257166208 22.003208523776372 18.61730786693707 36-37 20.458631732521322 39.774316380829 21.976305641485105 17.79074624516457 38-39 22.20769314590825 40.11962272831838 21.315365708958385 16.357318416814984 40-41 20.225807428035232 41.702333747010925 22.147894784635408 15.923964040318431 42-43 18.544056663740655 43.32536247237945 22.023186124648124 16.10739473923177 44-45 18.436298695402126 43.79695492932167 20.906862003208524 16.859884372067683 46-47 18.355288647810927 43.62831751259202 20.435393258426966 17.581000581170088 48-49 18.322870443800056 43.6475025274386 20.424125385169535 17.605501643591808 50-51 17.590580258498047 45.07067833035687 20.171323062021372 17.167418349123707 52-53 17.120803947089627 44.87695614008536 20.58661500741593 17.415624905409086 54-55 16.666868461836497 45.2561991476172 20.81981305695467 17.257119333591632 56-57 16.88409964585162 45.59675515331295 19.25598571298847 18.26315948784696 58-59 17.207874759362173 44.165970481759956 19.66813168186166 18.95802307701621 60-61 17.490798140255713 43.24813541263076 20.26285838822162 18.9982080588919 62-63 18.6857160153767 42.57468898507734 19.726972788086087 19.012622211459878 64-65 18.578070515304145 41.821847152266564 20.804073033707866 18.796009298721426 66-67 18.642508187690314 41.371899725765346 20.506456319201874 19.479135767342466 68-69 18.620601980773422 41.40555003995448 20.504516066542365 19.469331912729736 70-71 19.203690363043098 40.5728053660397 20.071192042957374 20.152312227959825 72-73 19.245344698162096 39.903019589800714 20.178826549144006 20.672809162893188 74-75 19.9112526333632 39.77589171126232 20.81930890863743 19.49354674673705 76-77 20.609245223623994 38.45618325786377 22.439886674576844 18.494684843935396 78-79 20.249049567765212 38.18618785868223 22.924183354723105 18.640579218829455 80-81 19.669709664140253 39.11482674286268 22.614838850279682 18.60062474271739 82-83 19.77867641717316 37.36591035668451 22.603336804126208 20.252076422016128 84-85 19.79623217182846 36.26837300530305 23.38063297576095 20.55476184710754 86-87 20.753928856817687 35.41116788144417 23.71903528101315 20.115867980724992 88-89 21.315107634937164 34.394144853137036 24.26629052957842 20.024456982347385 90-91 20.420974889217135 35.046734629634116 24.449717897183813 20.082572583964936 92-93 19.745986391263287 36.126716226360266 23.805603312589295 20.321694069787153 94-95 18.47410223502918 37.89682059229484 22.763760079424657 20.865317093251328 96-97 18.498922439886677 38.33813594207812 22.042763396856916 21.120178221178293 98-99 18.04731578565029 39.78920986996634 21.49429740659128 20.66917693779209 100-101 17.364667707917135 41.584637923310694 21.767583209230807 19.283111159541367 102-103 16.340268907359537 43.475576165194596 21.525907002367013 18.65824792507885 104-105 15.181582085757354 45.61315357746069 21.187502648513505 18.017761688268447 106-107 14.999878925829963 47.594256241373465 20.115262609874808 17.290602222921763 108-109 14.701431096689833 48.4284572729254 19.079473085212 17.790638545172772 110-111 14.686385732533433 48.87975446009674 19.17100013923614 17.26285966813369 112-113 15.59980143836114 48.976317892340845 18.906942392909897 16.516938276388117 114-115 15.137903479671646 49.18335472310337 19.050415284403226 16.628326512821754 116-117 14.89393902704797 48.928493595176406 19.219919122454414 16.95764825532121 118-119 14.994430588178318 48.681502288301814 19.228394314356976 17.095672809162892 120-121 15.396396832699713 47.79705547618471 19.03891323824975 17.767634452865828 122-123 15.678499648884909 46.559677458411024 18.712012979151027 19.04980991355304 124-125 16.062910138751 46.96103833208223 18.760442647165654 18.215608882001113 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 497.0 1 669.0 2 920.0 3 915.5 4 719.5 5 538.5 6 426.0 7 371.5 8 357.0 9 349.5 10 298.5 11 240.5 12 224.5 13 205.0 14 183.5 15 174.5 16 177.0 17 169.5 18 182.0 19 226.5 20 270.0 21 331.0 22 381.0 23 457.0 24 546.0 25 675.0 26 844.5 27 993.5 28 1106.5 29 1239.5 30 1547.5 31 1810.0 32 2031.0 33 2377.0 34 2793.5 35 3016.5 36 3075.0 37 3286.5 38 3341.0 39 3186.0 40 2915.5 41 2597.5 42 2304.5 43 2124.5 44 2124.5 45 2147.5 46 2076.5 47 2156.5 48 2763.5 49 2648.5 50 2220.5 51 2052.5 52 1585.5 53 1385.5 54 1258.5 55 1073.5 56 936.0 57 857.0 58 803.0 59 735.5 60 662.5 61 630.0 62 557.0 63 393.5 64 296.5 65 269.5 66 232.5 67 198.0 68 175.5 69 162.5 70 173.5 71 157.5 72 149.0 73 198.0 74 180.5 75 112.0 76 76.0 77 68.0 78 67.5 79 50.5 80 27.0 81 14.5 82 14.0 83 13.0 84 7.5 85 7.5 86 6.5 87 3.0 88 3.0 89 3.0 90 3.0 91 2.0 92 2.5 93 2.0 94 1.5 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.001210741700365644 9 0.001210741700365644 10-11 0.0018161125505484661 12-13 0.0018161125505484661 14-15 0.0036322251010969323 16-17 0.004237595951279754 18-19 0.004237595951279754 20-21 0.004842966801462576 22-23 0.09262174007797178 24-25 0.002421483400731288 26-27 0.00302685425091411 28-29 0.0018161125505484661 30-31 0.001210741700365644 32-33 0.002421483400731288 34-35 0.0018161125505484661 36-37 6.05370850182822E-4 38-39 0.001210741700365644 40-41 0.0018161125505484661 42-43 0.0018161125505484661 44-45 0.0018161125505484661 46-47 0.002421483400731288 48-49 6.05370850182822E-4 50-51 0.0018161125505484661 52-53 0.0018161125505484661 54-55 0.002421483400731288 56-57 0.0018161125505484661 58-59 0.001210741700365644 60-61 0.002421483400731288 62-63 0.0018161125505484661 64-65 0.002421483400731288 66-67 6.05370850182822E-4 68-69 0.0 70-71 6.05370850182822E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.001210741700365644 102-103 6.05370850182822E-4 104-105 6.05370850182822E-4 106-107 0.0 108-109 0.0 110-111 6.05370850182822E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 82594.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.13105068164757 #Duplication Level Percentage of deduplicated Percentage of total 1 78.36900847704041 42.42196769741143 2 8.727549263011921 9.448628229653485 3 4.556129638327853 7.39884253093445 4 2.8003310295466237 6.063394435431145 5 1.6529110469927757 4.473690582851055 6 1.0579525375204097 3.4360849456376976 7 0.6642957793732805 2.517131995060174 8 0.47194077255138783 2.043731990217207 9 0.31537274374287055 1.5364312177640023 >10 1.27938446397817 11.188464053078915 >50 0.049207094768391156 1.7555754655301836 >100 0.049207094768391156 5.114172942344481 >500 0.004473372251671923 1.3342373538029397 >1k 0.0022366861258359613 1.2676465602828293 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1047 1.2676465602828293 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 593 0.7179698283168269 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 509 0.6162675254861129 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 486 0.588420466377703 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 305 0.3692762186115214 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 282 0.34142915950311165 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 277 0.3353754510012834 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 253 0.3063176501925079 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 246 0.29784245828994843 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 238 0.28815652468702324 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 237 0.2869457829866576 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 218 0.2639416906797104 No Hit GAACAAAAAACACCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 180 0.2179335060658159 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 142 0.17192532145192144 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 141 0.17071457975155582 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 0.1682930963508245 No Hit GAACAAAAAAACACAAAGAACATGGGACTCCCGACCCGGGGAGGTAGTGA 138 0.16708235465045887 No Hit GAACAAAAAAACACAAAAAACATGGGACTCCCGACCCGGGGAGGTAGTGA 136 0.1646608712497276 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 135 0.16345012954936194 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 124 0.15013197084533986 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 117 0.14165677894278034 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 111 0.1343923287405865 No Hit GAACAAAAAAACACAGAGAACATGGGACTCCCGACCCGGGGAGGTAGTGA 109 0.1319708453398552 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 107 0.1295493619391239 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 103 0.12470639513766134 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.001816112550548466 0.0 24-25 0.0 0.0 0.0 0.0030268542509141096 0.0 26-27 0.0 0.0 0.0 0.005448337651645398 0.0 28-29 0.0 0.0 0.0 0.012107417003656439 0.0 30-31 0.0 0.0 0.0 0.03390076761023803 0.0 32-33 0.0 0.0 0.0 0.07809283967358405 0.0 34-35 0.0 0.0 0.0 0.12954936193912392 0.0 36-37 0.0 0.0 0.0 0.18403273845557788 0.0 38-39 0.0 0.0 0.0 0.29542097488921715 0.0 40-41 0.0 0.0 0.0 0.421943482577427 0.0 42-43 0.0 0.0 0.0 0.5460445068649054 0.0 44-45 0.0 0.0 0.0 0.6495629222461681 0.0 46-47 0.0 0.0 0.0 0.7670048671816354 0.0 48-49 0.0 0.0 0.0 0.9153207254764268 0.0 50-51 0.0 0.0 0.0 1.0363948955129914 0.0 52-53 0.0 0.0 0.0 1.1889483497590625 0.0 54-55 0.0 0.0 0.0 1.3396856914545852 0.0 56-57 0.0 0.0 0.0 1.5146378671574205 0.0 58-59 0.0 0.0 0.0 1.7174371019686658 0.0 60-61 0.0 0.0 0.0 1.9208417076300943 0.0 62-63 0.0 0.0 0.0 2.081264982928542 0.0 64-65 0.0 0.0 0.0 2.2598493837324742 0.0 66-67 0.0 0.0 0.0 2.4541734266411606 0.0 68-69 0.0 0.0 0.0 2.7102452962684938 0.0 70-71 0.0 0.0 0.0 2.9711601326972903 0.0 72-73 0.0 0.0 0.0 3.1951473472649345 0.0 74-75 0.0 0.0 0.0 3.471801825798484 0.0 76-77 0.0 0.0 0.0 3.7454294500811196 0.0 78-79 0.0 0.0 0.0 4.0584061796256385 0.0 80-81 0.0 0.0 0.0 4.3949923723272875 0.0 82-83 0.0 0.0 0.0 4.724919485676926 0.0 84-85 0.0 0.0 0.0 5.1287018427488675 0.0 86-87 0.0 0.0 0.0 5.551856067026661 0.0 88-89 0.0 0.0 0.0 5.959876020049883 0.0 90-91 0.0 0.0 0.0 6.344891880766157 0.0 92-93 0.0 0.0 0.0 6.75957091314139 0.0 94-95 0.0 0.0 0.0 7.1373223236554715 0.0 96-97 0.0 0.0 0.0 7.584086011090394 0.0 98-99 0.0 0.0 0.0 8.085938445891953 0.0 100-101 0.0 0.0 0.0 8.637431290408504 0.0 102-103 0.0 0.0 0.0 9.188318764074872 0.0 104-105 0.0 0.0 0.0 9.690776569726616 0.0 106-107 0.0 0.0 0.0 10.172651766472141 0.0 108-109 0.0 0.0 0.0 10.794973000460082 0.0 110-111 0.0 0.0 0.0 11.456643339709906 0.0 112-113 0.0 0.0 0.0 12.172191684626002 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAA 145 0.0 94.3625 1 CTAGACT 20 8.459506E-4 89.2341 4 TACAAGG 40 1.880835E-8 89.2341 2 GGAGATA 30 4.2445266E-5 79.3192 8 CAGTTGA 25 0.0020515197 71.387276 1 GTAGCCC 25 0.0020515197 71.387276 3 AGGCATC 25 0.0020515197 71.387276 6 CAACTCA 25 0.0020515197 71.387276 6 AAGGCAT 25 0.0020515197 71.387276 5 GAACAAA 560 0.0 69.0502 1 GTACATA 70 1.72804E-10 67.987885 1 TACATAA 45 3.960382E-6 66.099335 2 AGAACAA 285 0.0 60.533073 2 TACATGA 40 1.7638673E-4 59.489403 2 CCCTCGG 15 0.0042331237 59.4894 48-49 CCTAGAC 30 0.004225636 59.4894 3 GAGAACA 440 0.0 55.433308 1 TCCTAGA 45 3.1564655E-4 52.879467 2 ACATGGA 160 0.0 52.053226 3 TAGACTG 35 0.0077762683 50.990917 5 >>END_MODULE