##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936191_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82594 Sequences flagged as poor quality 0 Sequence length 125 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23027096399254 31.0 31.0 33.0 30.0 34.0 2 31.040535632128243 31.0 31.0 34.0 28.0 34.0 3 31.26149599244497 31.0 31.0 34.0 28.0 34.0 4 35.2499939462915 35.0 35.0 37.0 33.0 37.0 5 35.02072789791026 35.0 35.0 37.0 33.0 37.0 6 35.31480494951207 37.0 35.0 37.0 33.0 37.0 7 35.10112114681454 37.0 35.0 37.0 32.0 37.0 8 35.26233140421822 37.0 35.0 37.0 32.0 37.0 9 36.77058866261472 39.0 37.0 39.0 32.0 39.0 10-11 36.70226045475458 39.0 36.0 39.0 32.0 39.0 12-13 36.58899556868538 39.0 35.0 39.0 32.0 39.0 14-15 37.17604789694167 39.0 36.0 41.0 31.5 41.0 16-17 37.18716855945952 39.0 36.0 40.5 31.5 41.0 18-19 37.77345812044459 39.5 37.0 41.0 32.0 41.0 20-21 37.9450323268034 40.0 37.0 41.0 33.0 41.0 22-23 37.67145918589728 40.0 36.5 41.0 32.0 41.0 24-25 37.445026273094896 40.0 36.0 41.0 32.0 41.0 26-27 36.986923989636054 39.0 35.0 41.0 31.5 41.0 28-29 36.60027362762428 39.0 35.0 41.0 31.0 41.0 30-31 35.682289270407054 37.5 35.0 40.0 30.0 41.0 32-33 34.5400513354481 35.5 33.5 40.0 28.0 41.0 34-35 33.665078577136356 35.0 33.0 40.0 23.5 41.0 36-37 32.83843862750321 35.0 33.0 40.0 17.0 41.0 38-39 32.361230840012595 35.0 33.0 40.0 7.5 41.0 40-41 31.754031769862216 35.0 33.0 40.0 2.0 41.0 42-43 31.011380971983435 35.0 32.0 40.0 2.0 41.0 44-45 29.840581640312855 35.0 28.0 40.0 2.0 41.0 46-47 27.92821512458532 35.0 14.0 40.0 2.0 41.0 48-49 26.285359711359177 35.0 2.0 40.0 2.0 41.0 50-51 25.704088674722136 35.0 2.0 40.0 2.0 41.0 52-53 25.53092234302734 35.0 2.0 40.0 2.0 41.0 54-55 25.39870934934741 35.0 2.0 39.5 2.0 41.0 56-57 25.250502457805652 34.5 2.0 39.0 2.0 41.0 58-59 25.059750102913043 34.0 2.0 39.0 2.0 41.0 60-61 24.839982323171174 34.0 2.0 39.0 2.0 41.0 62-63 24.57904932561687 34.0 2.0 38.5 2.0 41.0 64-65 24.285807685788313 33.5 2.0 38.0 2.0 40.5 66-67 23.98834055742548 33.0 2.0 37.5 2.0 40.0 68-69 23.744248976923263 33.0 2.0 37.0 2.0 40.0 70-71 23.43969900961329 33.0 2.0 36.5 2.0 39.5 72-73 23.131831610044316 33.0 2.0 36.0 2.0 39.0 74-75 22.908237886529285 33.0 2.0 36.0 2.0 39.0 76-77 22.58163425914715 32.5 2.0 35.0 2.0 39.0 78-79 22.24295348330387 32.0 2.0 35.0 2.0 37.5 80-81 21.963617211904015 32.0 2.0 35.0 2.0 37.0 82-83 21.6745042012737 31.5 2.0 35.0 2.0 36.5 84-85 21.364705668692643 31.0 2.0 35.0 2.0 36.0 86-87 21.047739545245417 31.0 2.0 35.0 2.0 36.0 88-89 20.767573915780808 30.5 2.0 35.0 2.0 35.5 90-91 20.518130856962976 30.0 2.0 35.0 2.0 35.0 92-93 20.347501029130445 30.0 2.0 35.0 2.0 35.0 94-95 20.195383441896503 30.0 2.0 35.0 2.0 35.0 96-97 20.014407826234354 29.5 2.0 35.0 2.0 35.0 98-99 19.83302055839407 29.0 2.0 35.0 2.0 35.0 100-101 19.691835968714436 29.0 2.0 35.0 2.0 35.0 102-103 18.837833256653028 26.0 2.0 34.0 2.0 35.0 104-105 19.24513887207303 28.0 2.0 34.5 2.0 35.0 106-107 19.300215512022664 29.0 2.0 35.0 2.0 35.0 108-109 19.215675472794636 29.0 2.0 35.0 2.0 35.0 110-111 19.108385597016735 28.0 2.0 35.0 2.0 35.0 112-113 18.961667917766423 27.0 2.0 35.0 2.0 35.0 114-115 18.80212243020074 27.0 2.0 35.0 2.0 35.0 116-117 18.64858222146887 26.0 2.0 35.0 2.0 35.0 118-119 18.51210136329515 25.0 2.0 35.0 2.0 35.0 120-121 18.323867351139306 25.0 2.0 35.0 2.0 35.0 122-123 18.07553212097731 24.0 2.0 35.0 2.0 35.0 124-125 16.637201249485436 10.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18848466995963875 1101 2 0.7522804051752097 1101 3 0.6185228571117207 1101 4 0.18372229455353306 1101 5 0.09497913916896294 1101 6 0.20839620574415108 1101 7 0.7098860984374227 1101 8 0.4756872171483266 1101 9 0.8391098493320897 1101 10-11 0.7285124171051436 1101 12-13 0.8052205928012199 1101 14-15 0.8201578932024631 1101 16-17 0.58147165066611 1101 18-19 0.4604129295556021 1101 20-21 0.6986571554135779 1101 22-23 0.6500115510707118 1101 24-25 0.5273448558278275 1101 26-27 0.4461823471338704 1101 28-29 1.114211920421205 1101 30-31 0.6080053696333394 1101 32-33 0.1151992090889351 1101 34-35 0.3508082698430073 1101 36-37 0.6771352187242101 1101 38-39 0.08733015164015967 1101 40-41 -0.42088790406142707 1101 42-43 -0.529769643032278 1101 44-45 -0.30543933707555126 1101 46-47 -0.5158830373591918 1101 48-49 -1.3719898274362947 1101 50-51 -1.61141510751564 1101 52-53 -1.316246666703698 1101 54-55 -1.5232646103109353 1101 56-57 -1.5495011079775338 1101 58-59 -1.3854460046656705 1101 60-61 -1.5018454330132123 1101 62-63 -1.7165889748238143 1101 64-65 -1.4232892093374048 1101 66-67 -1.5361614318784333 1101 68-69 -1.4464206441862544 1101 70-71 -1.5004688529551267 1101 72-73 -1.2710955876659753 1101 74-75 -1.474119911209403 1101 76-77 -1.5113990159228443 1101 78-79 -1.5718042564844161 1101 80-81 -1.1309953757481281 1101 82-83 -1.080667168261396 1101 84-85 -1.2867953801871366 1101 86-87 -1.5295175359355468 1101 88-89 -1.5690173981640356 1101 90-91 -1.6837551731365856 1101 92-93 -1.9304702005199594 1101 94-95 -1.8029433479282417 1101 96-97 -1.5946322199176919 1101 98-99 -1.9535691100010482 1101 100-101 -1.6378356710784487 1101 102-103 -1.6838063971734556 1101 104-105 -1.721190753468683 1101 106-107 -1.7422101687988985 1101 108-109 -1.6663357081890346 1101 110-111 -1.7963083205810548 1101 112-113 -1.8344073179073526 1101 114-115 -2.085413170901578 1101 116-117 -2.583162425732521 1101 118-119 -1.7915728058107376 1101 120-121 -1.9977432748961483 1101 122-123 -2.1585753866146433 1101 124-125 -1.9783833040808716 1102 1 -0.018019395081012135 1102 2 0.53547823715353 1102 3 0.3003656755399078 1102 4 -0.357560451607263 1102 5 0.26860154205965614 1102 6 0.19177470619582238 1102 7 0.3561372276153847 1102 8 0.3566266932097548 1102 9 0.19900144824807597 1102 10-11 0.41984033038427526 1102 12-13 0.20196856028088916 1102 14-15 0.40308472247075144 1102 16-17 0.18833705265346623 1102 18-19 0.12301455557185648 1102 20-21 0.24888299100526012 1102 22-23 0.3713304309261716 1102 24-25 0.27043428672214276 1102 26-27 0.24103329564336207 1102 28-29 0.23670514535345433 1102 30-31 -0.3070804478915079 1102 32-33 -0.16013954429859467 1102 34-35 0.16110637282403673 1102 36-37 0.2817422647946657 1102 38-39 0.8877818228235341 1102 40-41 0.04397207787172874 1102 42-43 -0.8389837351731977 1102 44-45 -0.9915278646274928 1102 46-47 0.022328344754626528 1102 48-49 -0.6190539647443352 1102 50-51 -0.6543238699365972 1102 52-53 -0.6998961698653972 1102 54-55 -0.9662637069685687 1102 56-57 -0.6163484431175519 1102 58-59 -0.3137206207451655 1102 60-61 -0.8627307085326343 1102 62-63 -0.8025871681390839 1102 64-65 -0.5655656321016345 1102 66-67 -0.27301780044211554 1102 68-69 -0.31055795222600224 1102 70-71 -0.9247687626208894 1102 72-73 -0.9699212425891908 1102 74-75 -0.646025963603261 1102 76-77 -0.7121216898162466 1102 78-79 -1.2527437325458415 1102 80-81 -1.592332322450929 1102 82-83 -1.2859065539885854 1102 84-85 -1.6640311525448617 1102 86-87 -1.5746846542733977 1102 88-89 -0.8893426014160681 1102 90-91 -0.6338003402549219 1102 92-93 -0.6544087732390196 1102 94-95 -0.5859605114332105 1102 96-97 -0.7619312262410887 1102 98-99 -0.8135510431537156 1102 100-101 -0.8425330513855869 1102 102-103 -0.7422526483026353 1102 104-105 -1.0091762999908163 1102 106-107 -1.2852092654565297 1102 108-109 -1.0438424832567854 1102 110-111 -0.8359650504816862 1102 112-113 -1.4119140929751026 1102 114-115 -1.4008603253731273 1102 116-117 -1.4130630580721757 1102 118-119 -1.279287349622841 1102 120-121 -1.0644099415628183 1102 122-123 -0.8749258834529456 1102 124-125 -1.1663688506030017 1103 1 0.028134451072833855 1103 2 0.6414611431364357 1103 3 0.4123314875057176 1103 4 0.6455734515266442 1103 5 0.587974761432875 1103 6 0.17268638710749684 1103 7 0.12764719912535583 1103 8 0.3349742715573285 1103 9 -0.03262248337585305 1103 10-11 -0.04853573799179145 1103 12-13 0.012224970537303648 1103 14-15 0.9671872865733206 1103 16-17 0.8561433204597364 1103 18-19 0.26660429916160666 1103 20-21 -0.16450732238504884 1103 22-23 -0.017843358247617402 1103 24-25 0.1947932610811094 1103 26-27 0.3581273127373805 1103 28-29 0.2768760855243926 1103 30-31 0.3966232558121945 1103 32-33 0.5214843873253372 1103 34-35 0.20255936427702892 1103 36-37 0.40909269214510147 1103 38-39 0.7289499139916273 1103 40-41 1.0908381747378222 1103 42-43 0.8191359229464581 1103 44-45 0.2510647279650975 1103 46-47 1.8979693703956535 1103 48-49 1.078666832976463 1103 50-51 1.151374135761408 1103 52-53 1.5837220637528375 1103 54-55 1.2891493984445361 1103 56-57 1.352312525543418 1103 58-59 1.0381312311066857 1103 60-61 0.8670413712394414 1103 62-63 1.4197775042255927 1103 64-65 1.585716419180418 1103 66-67 1.9633069858826673 1103 68-69 1.5743423326742807 1103 70-71 1.0267981889460636 1103 72-73 1.3658337431657976 1103 74-75 0.8643728967956008 1103 76-77 0.967650389955832 1103 78-79 1.0689086891065784 1103 80-81 1.1937075635889585 1103 82-83 1.7116717935897618 1103 84-85 2.0551996166859077 1103 86-87 2.189275459686719 1103 88-89 1.6170676549941874 1103 90-91 1.9070828506282673 1103 92-93 2.3829131640829146 1103 94-95 2.853498178025479 1103 96-97 2.6001770358671727 1103 98-99 2.966505936903264 1103 100-101 2.5170111081585738 1103 102-103 2.9362373801873964 1103 104-105 3.159484668670153 1103 106-107 3.145844865597603 1103 108-109 3.0270976876833835 1103 110-111 3.2440919295752906 1103 112-113 3.766718385657377 1103 114-115 3.7183704438576406 1103 116-117 3.7221221271130105 1103 118-119 3.76330524296975 1103 120-121 3.7496926225397473 1103 122-123 3.8927379342108708 1103 124-125 3.401010066775914 1104 1 0.10737743031581459 1104 2 0.40055760223289383 1104 3 0.28131805649228525 1104 4 0.23714854310173195 1104 5 0.5680724415305534 1104 6 0.6245789390000525 1104 7 0.4206874921656478 1104 8 0.5687960053790633 1104 9 0.7212236704703017 1104 10-11 0.5158720764160236 1104 12-13 0.5940320523443816 1104 14-15 0.709433928819962 1104 16-17 0.5714058357222527 1104 18-19 0.31745900001629934 1104 20-21 0.21607875820103573 1104 22-23 -0.06253200293625838 1104 24-25 0.22837079465863752 1104 26-27 0.33468408929415716 1104 28-29 0.22241943106773476 1104 30-31 -0.15893229974336265 1104 32-33 0.013669979510929409 1104 34-35 0.07777303949070102 1104 36-37 -0.015083132030724755 1104 38-39 -0.2270329919912797 1104 40-41 0.3881519720516273 1104 42-43 -0.15723770342716392 1104 44-45 -0.6687765418761735 1104 46-47 -1.85386213143585 1104 48-49 -1.6137629594533287 1104 50-51 -1.5921545577672838 1104 52-53 -1.3128591328283576 1104 54-55 -1.3006552413601007 1104 56-57 -1.039628866397976 1104 58-59 -0.8721862292107758 1104 60-61 -1.2423603381622677 1104 62-63 -1.2277194432713578 1104 64-65 -1.0893751559111564 1104 66-67 -2.0261924036167187 1104 68-69 -2.0489177405857895 1104 70-71 -2.0575729954251223 1104 72-73 -1.5416143642823137 1104 74-75 -1.211634429211724 1104 76-77 -1.5988941765887326 1104 78-79 -1.8831670129691211 1104 80-81 -1.8547661848847916 1104 82-83 -1.5165943846764165 1104 84-85 -1.0227613112750191 1104 86-87 -0.8749492045379483 1104 88-89 -0.8948981569716246 1104 90-91 -1.8777156841702656 1104 92-93 -1.4993823182125645 1104 94-95 -1.3420451675178633 1104 96-97 -1.1711904855003468 1104 98-99 -1.0251912547939277 1104 100-101 -0.9486177074702411 1104 102-103 -0.8552156112655958 1104 104-105 -0.9015043423188587 1104 106-107 -0.8569023871496526 1104 108-109 -1.1771758165901218 1104 110-111 -1.375383039899674 1104 112-113 -1.9444537755147842 1104 114-115 -2.209590484103284 1104 116-117 -1.8937508887600067 1104 118-119 -2.506536026871519 1104 120-121 -2.3628226399755157 1104 122-123 -2.4516454601725215 1104 124-125 -2.3967921310262827 1105 1 0.044391952436718896 1105 2 0.9019084972008109 1105 3 0.36372264953517686 1105 4 0.5056389179750838 1105 5 0.7308552851644663 1105 6 0.3263688748325393 1105 7 0.3153176846681802 1105 8 0.29405774127909723 1105 9 0.22831586905186185 1105 10-11 0.12385924291382366 1105 12-13 -0.11079739716591774 1105 14-15 0.17329748842820436 1105 16-17 0.7509060045841167 1105 18-19 0.449255690323632 1105 20-21 0.46227936610377185 1105 22-23 0.904033346607811 1105 24-25 0.3817818044526362 1105 26-27 0.504390269638634 1105 28-29 0.7901330413345349 1105 30-31 1.0030062345355972 1105 32-33 0.8935247583018722 1105 34-35 0.9400661836987396 1105 36-37 1.2989999480098007 1105 38-39 1.9266313106091957 1105 40-41 1.7892833465872506 1105 42-43 1.0350115366944124 1105 44-45 1.9450363591707713 1105 46-47 1.6400588412085249 1105 48-49 1.6933967838766257 1105 50-51 1.4563932301422042 1105 52-53 1.822483656769748 1105 54-55 1.950442360801329 1105 56-57 2.2268351660235055 1105 58-59 1.9055071176315082 1105 60-61 1.7795072741308786 1105 62-63 1.9790519177553207 1105 64-65 1.5656400416572325 1105 66-67 1.822254067170178 1105 68-69 2.1706871226360924 1105 70-71 2.058603971815675 1105 72-73 1.8529313972847277 1105 74-75 2.004171041912894 1105 76-77 1.8811801226345004 1105 78-79 1.719754297398996 1105 80-81 1.7188575908666444 1105 82-83 1.85507059337154 1105 84-85 2.17497593965372 1105 86-87 2.0722814607778233 1105 88-89 2.134061653903082 1105 90-91 2.0138749946544543 1105 92-93 1.5723839769579868 1105 94-95 1.877475264768524 1105 96-97 1.902686583057573 1105 98-99 2.0950383973506206 1105 100-101 2.0823684458563356 1105 102-103 1.7623966818786094 1105 104-105 1.4113122736892478 1105 106-107 1.8910712703984736 1105 108-109 1.7932733559867096 1105 110-111 1.3541846737105914 1105 112-113 1.582648554779034 1105 114-115 0.9225439299460234 1105 116-117 0.5955263824321584 1105 118-119 0.14805701601939347 1105 120-121 0.7185687818414372 1105 122-123 0.9083680487771559 1105 124-125 0.9519920635025905 1106 1 0.3684467703325218 1106 2 0.626539222951358 1106 3 0.5486530606693947 1106 4 0.12186032255035428 1106 5 0.7618903195589581 1106 6 0.37228152880790333 1106 7 0.5510133067019893 1106 8 0.38876258850353906 1106 9 0.28137404641014996 1106 10-11 0.4254376570342373 1106 12-13 0.709737983839787 1106 14-15 0.4416812136988284 1106 16-17 0.12988536788599703 1106 18-19 0.8122376132159701 1106 20-21 0.7511664774992823 1106 22-23 1.1003752652341632 1106 24-25 0.8551460661707537 1106 26-27 0.844709151950795 1106 28-29 1.2140652121872009 1106 30-31 1.1184277670903882 1106 32-33 0.13042018836640068 1106 34-35 0.09761430933196635 1106 36-37 0.6240146123301784 1106 38-39 0.7362084449343627 1106 40-41 0.34575431123290556 1106 42-43 0.855920191309675 1106 44-45 0.8341474347320137 1106 46-47 -0.5105037354458766 1106 48-49 -0.8222007205226696 1106 50-51 -1.2423216421448977 1106 52-53 -1.2829928003304474 1106 54-55 -1.1498615905664522 1106 56-57 -1.3541652072501051 1106 58-59 -0.8799138816752681 1106 60-61 -0.7601130532834013 1106 62-63 -0.7442190255604118 1106 64-65 -1.2560418225778243 1106 66-67 -0.880620139623403 1106 68-69 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-0.5325591264520426 2216 124-125 -0.5515540357881861 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 13.0 4 30.0 5 83.0 6 283.0 7 491.0 8 841.0 9 1419.0 10 2365.0 11 2677.0 12 3115.0 13 4176.0 14 5804.0 15 4036.0 16 1308.0 17 1050.0 18 793.0 19 786.0 20 770.0 21 882.0 22 888.0 23 1028.0 24 1057.0 25 1152.0 26 1153.0 27 1216.0 28 1344.0 29 1534.0 30 1776.0 31 2079.0 32 2463.0 33 3000.0 34 3893.0 35 5394.0 36 7837.0 37 10952.0 38 4881.0 39 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.425116910179064 15.334011775823218 15.701097623028277 13.539773690969445 2 20.107030862179602 26.239511823035848 34.996912571283275 18.656544743501264 3 18.204712206697824 31.215342518827033 38.108095019008644 12.4718502554665 4 9.45831416325641 21.672276436545026 33.65014407826234 35.21926532193621 5 6.405922479493051 43.87214811045279 35.05870379121079 14.663225618843374 6 21.155289730488896 40.094922149308665 16.67675618083638 22.073031939366057 7 15.997530086931256 41.65677894278035 23.54529384701068 18.80039712327772 8 28.1013148654866 32.16577475361406 19.96149841392837 19.771411966970966 9 24.155507663994964 10.495919800469768 21.850255466498776 43.49831706903649 10-11 23.893382085865802 26.72409618132068 26.643581858246364 22.73893987456716 12-13 29.956171150446764 28.84228878611037 29.19098239581568 12.010557667627188 14-15 23.823764438094777 26.84638109305761 36.263530038501585 13.066324430346029 16-17 13.215851030341186 27.130300021793353 39.63605104487009 20.017797902995373 18-19 12.43794948785626 18.60546770951885 52.428142480083295 16.528440322541588 20-21 13.919291958253627 17.389277671501564 55.54882921277574 13.142601157469066 22-23 13.07722110564932 17.902026781606413 55.23766859578178 13.783083516962492 24-25 12.720657674891639 17.927452357314092 56.5440588904763 12.807831077317966 26-27 13.410175073249873 17.121098384870574 56.321282417609034 13.147444124270526 28-29 12.915587088650508 16.763324212412524 56.91999418843984 13.40109451049713 30-31 13.010024941279028 17.998886117635664 56.43569750829358 12.55539143279173 32-33 12.818122381771074 18.1913940479938 55.20013560307044 13.790347967164685 34-35 12.787853839261931 17.583601714410246 55.042133811172725 14.586410635155096 36-37 14.307334673220815 17.495217570283554 53.26597573673632 14.931472019759303 38-39 15.10642419546214 17.407438797007046 51.914182628278084 15.57195437925273 40-41 14.077293750151343 18.037629852047367 51.780395670387676 16.104680727413616 42-43 13.978436037606775 19.273775146352833 49.53475841944994 17.213030396590447 44-45 14.23425059788696 19.60161051070142 46.253140798595346 19.910998092816275 46-47 14.947414309846874 19.740976876827784 42.494808033470775 22.816800779854564 48-49 16.958901455522994 20.571068755903223 37.77518103218619 24.694848756387593 50-51 17.445373470487446 21.44435631815072 35.735346649149044 25.374923562212793 52-53 16.24629169946116 22.088151601380396 35.081431252648784 26.584125446509653 54-55 16.228030344309836 21.9166802889127 34.92925513558676 26.926034231190705 56-57 17.10528705570388 21.4854368344261 33.17229396344528 28.236982146424744 58-59 17.440262499924327 20.122169014596107 33.56499839569926 28.872570089780304 60-61 18.970736018790937 19.846112866707834 33.99803857469762 27.18511253980361 62-63 21.850387742376824 19.526354979508074 34.23574494360936 24.387512334505743 64-65 21.82254051468941 20.08329953325625 35.71588563264664 22.3782743194077 66-67 21.10737117396358 20.36698469585432 36.36550755521116 22.16013657497094 68-69 20.62559023657893 21.295735767731312 36.288955614209264 21.789718381480494 70-71 19.24858185868835 21.61447139804215 36.14338210811171 22.993564635157796 72-73 18.32463585620709 20.900582387911516 36.340521364313304 24.43426039156809 74-75 17.30584125869469 21.60823793639935 37.02712683201463 24.05879397289133 76-77 17.208981336303705 21.284362570904488 38.67495626169129 22.83169983110051 78-79 16.596847228612248 21.555439862459743 39.57127636390052 22.276436545027483 80-81 16.0840981185074 22.731675424364965 38.75281497445335 22.431411482674285 82-83 15.992687120129792 22.537956752306464 37.486379155870885 23.98297697169286 84-85 16.416446715257766 21.623846768530402 37.34109015182701 24.61861636438482 86-87 17.030395854395323 21.951485286372414 36.792241520216486 24.225877339015785 88-89 17.298681486324508 21.122855447798237 36.92322593924425 24.655237126633008 90-91 16.493828057366677 22.292850959239146 36.81492647548477 24.39839450790941 92-93 16.07622829745502 23.12335036443325 35.27011647335158 25.53030486476015 94-95 16.138171515745885 23.73203540251595 34.335234220817746 25.794558860920418 96-97 17.036050488845838 23.380452220237917 32.763265429669765 26.82023186124648 98-99 17.136042231693143 23.41570612165827 31.692536807438977 27.75571483920961 100-101 17.898502908824547 23.069612015473375 32.07637404880529 26.95551102689679 102-103 17.756132406712354 22.82732401869385 31.93815531394532 27.478388260648472 104-105 17.847543405089958 23.13545778143691 31.676029735816165 27.340969077656972 106-107 17.822225719941642 23.95466955632102 31.11080169747014 27.1123030262672 108-109 18.446366844848566 24.145362528528274 29.522904344772893 27.885366281850267 110-111 18.636341622878174 24.07983630772211 29.141341986100684 28.142480083299027 112-113 19.615037630404643 23.969023789197077 29.019914143340657 27.396024437057626 114-115 19.600683089891724 24.184894508635516 29.01739214688855 27.19703025458421 116-117 18.910244764984437 24.281510009809978 29.436592425728787 27.371652799476802 118-119 19.00411118982314 24.853020422744144 29.395915451171295 26.74695293626142 120-121 19.01351318625433 24.967912236940887 28.269319255079555 27.74925532172523 122-123 19.309589107442317 24.843652566640635 27.798664462970024 28.04809386294702 124-125 19.22508778302458 24.557452476086695 28.18682649231142 28.03063324857731 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 83.0 1 63.0 2 97.5 3 175.5 4 162.0 5 163.5 6 242.0 7 563.5 8 915.5 9 1100.0 10 1012.5 11 724.0 12 617.0 13 568.5 14 543.5 15 545.0 16 492.5 17 443.0 18 450.0 19 460.5 20 474.5 21 486.5 22 479.5 23 506.0 24 567.0 25 615.5 26 718.5 27 889.5 28 970.0 29 1062.0 30 1233.0 31 1394.5 32 1677.5 33 2021.5 34 2349.0 35 2454.0 36 2629.0 37 3037.5 38 3167.0 39 3095.5 40 3038.0 41 2897.5 42 2702.5 43 2610.5 44 2587.0 45 2515.0 46 2383.0 47 2398.5 48 3083.5 49 2907.0 50 2292.0 51 2116.0 52 1581.0 53 1311.5 54 1169.0 55 1024.0 56 878.5 57 796.0 58 741.0 59 624.5 60 519.5 61 516.0 62 485.0 63 314.5 64 187.5 65 176.5 66 167.5 67 148.0 68 137.0 69 118.5 70 100.5 71 99.0 72 118.0 73 195.0 74 173.0 75 71.5 76 44.0 77 55.5 78 53.5 79 22.0 80 7.5 81 4.0 82 2.5 83 0.0 84 3.0 85 5.0 86 3.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06295856841901348 2 0.001210741700365644 3 0.0 4 0.0 5 0.07385524372230429 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.00302685425091411 44-45 0.013923529554204906 46-47 0.017555754655301837 48-49 0.014528900404387729 50-51 0.012712787853839261 52-53 0.010896675303290797 54-55 0.010291304453107974 56-57 0.006659079352011042 58-59 0.004237595951279754 60-61 0.001210741700365644 62-63 6.05370850182822E-4 64-65 6.05370850182822E-4 66-67 0.002421483400731288 68-69 0.0 70-71 0.004237595951279754 72-73 0.0036322251010969323 74-75 6.05370850182822E-4 76-77 6.05370850182822E-4 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 6.05370850182822E-4 88-89 0.001210741700365644 90-91 0.00302685425091411 92-93 0.0 94-95 0.001210741700365644 96-97 0.0018161125505484661 98-99 0.002421483400731288 100-101 6.05370850182822E-4 102-103 0.0 104-105 0.0 106-107 6.05370850182822E-4 108-109 6.05370850182822E-4 110-111 0.0 112-113 0.017555754655301837 114-115 0.03390076761023803 116-117 0.0302685425091411 118-119 0.017555754655301837 120-121 0.009685933602925152 122-123 0.006659079352011042 124-125 0.004842966801462576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 82594.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.973775334770075 #Duplication Level Percentage of deduplicated Percentage of total 1 69.03386144765456 26.905102065525337 2 11.711711711711711 9.128992420756955 3 6.349798073936006 7.424268106642129 4 4.228021124572848 6.591277816790566 5 2.680956818887853 5.224350437077754 6 1.5470643056849953 3.617696200692544 7 0.97856477166822 2.669685449306245 8 0.6554830692761727 2.043731990217207 9 0.521901211556384 1.8306414509528537 >10 2.0689655172413794 13.761290166355911 >50 0.1056228642435539 2.69874325011502 >100 0.1056228642435539 8.794827711456039 >500 0.003106554830692762 0.625953459089038 >1k 0.009319664492078286 8.683439475022398 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3025 3.6624936436060733 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2092 2.532871637164927 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2055 2.4880741942513986 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 517 0.625953459089038 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 481 0.5823667578758748 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 439 0.5315156064605177 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 418 0.5060900307528392 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 417 0.5048792890524736 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 397 0.48066445504516064 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 384 0.46492481294040733 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 310 0.3753299271133496 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 259 0.3135821003947018 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 257 0.3111606169939705 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 253 0.3063176501925079 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 249 0.3014746833910454 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 248 0.3002639416906797 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 238 0.28815652468702324 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 216 0.2615202072789791 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 204 0.24699130687459137 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 196 0.2373053732716662 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 191 0.231251664769838 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 161 0.19492941375886869 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 151 0.18282199675521224 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 147 0.17797902995374967 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 147 0.17797902995374967 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 131 0.15860716274789935 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 127 0.15376419594643678 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 121 0.14649974574424293 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 121 0.14649974574424293 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 119 0.14407826234351162 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 118 0.142867520643146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 114 0.1380245538416834 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG 113 0.13681381214131777 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 112 0.13560307044095213 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 110 0.13318158704022082 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 108 0.13076010363948956 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 105 0.1271278785383926 No Hit GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 102 0.12349565343729568 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 97 0.11744194493546747 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 90 0.10896675303290795 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 86 0.10412378623144537 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 84 0.10170230283071409 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.001816112550548466 0.0 24-25 0.0 0.0 0.0 0.0030268542509141096 0.0 26-27 0.0 0.0 0.0 0.005448337651645398 0.0 28-29 0.0 0.0 0.0 0.012107417003656439 0.0 30-31 0.0 0.0 0.0 0.03329539676005521 0.0 32-33 0.0 0.0 0.0 0.07627672712303557 0.0 34-35 0.0 0.0 0.0 0.1265225076882098 0.0 36-37 0.0 0.0 0.0 0.1816112550548466 0.0 38-39 0.0 0.0 0.0 0.2942102331888515 0.0 40-41 0.0 0.0 0.0 0.4243649659781582 0.0 42-43 0.0 0.0 0.0 0.5490713611158196 0.0 44-45 0.0 0.0 0.0 0.6538005181974478 0.0 46-47 0.0 0.0 0.0 0.7748746882340121 0.0 48-49 0.0 0.0 0.0 0.9262174007797177 0.0 50-51 0.0 0.0 0.0 1.051529166767562 0.0 52-53 0.0 0.0 0.0 1.2161900380172894 0.0 54-55 0.0 0.0 0.0 1.380850909267017 0.0 56-57 0.0 0.0 0.0 1.5666997602731434 0.0 58-59 0.0 0.0 0.0 1.7803956703876795 0.0 60-61 0.0 0.0 0.0 1.999539918153861 0.0 62-63 0.0 0.0 0.0 2.1750974647068793 0.0 64-65 0.0 0.0 0.0 2.3718429910162966 0.0 66-67 0.0 0.0 0.0 2.5794851926290043 0.0 68-69 0.0 0.0 0.0 2.8488752209603603 0.0 70-71 0.0 0.0 0.0 3.137637116497566 0.0 72-73 0.0 0.0 0.0 3.3949197278252656 0.0 74-75 0.0 0.0 0.0 3.703053490568322 0.0 76-77 0.0 0.0 0.0 4.003922803109185 0.0 78-79 0.0 0.0 0.0 4.348984187713393 0.0 80-81 0.0 0.0 0.0 4.740053756931497 0.0 82-83 0.0 0.0 0.0 5.1238588759474055 0.0 84-85 0.0 0.0 0.0 5.569411821681962 0.0 86-87 0.0 0.0 0.0 6.032520522071821 0.0 88-89 0.0 0.0 0.0 6.478073467806379 0.0 90-91 0.0 0.0 0.0 6.9115189965372785 0.0 92-93 0.0 0.0 0.0 7.357677313122019 0.0 94-95 0.0 0.0 0.0 7.784463762500908 0.001210741700365644 96-97 0.0 0.0 0.0 8.289948422403565 0.001210741700365644 98-99 0.0 0.0 0.0 8.833571445867738 0.001210741700365644 100-101 0.0 0.0 0.0 9.432888587548732 0.001210741700365644 102-103 0.0 0.0 0.0 10.032811100079908 0.001210741700365644 104-105 0.0 0.0 0.0 10.575223381843717 0.001210741700365644 106-107 0.0 0.0 0.0 11.100079908952225 0.001210741700365644 108-109 0.0 0.0 0.0 11.760539506501683 0.001210741700365644 110-111 0.0 0.0 0.0 12.492432864372715 0.001210741700365644 112-113 0.0 0.0 0.0 13.256410877303436 0.001210741700365644 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCACGCA 15 2.686509E-4 119.069084 4 GTAGGAC 20 8.4340305E-4 89.30182 3 TATAACG 25 0.0020453515 71.44145 2 CACGCAG 35 9.076151E-5 68.039474 5 GGTATCA 1970 0.0 59.836754 1 ATCCACG 40 1.7572581E-4 59.534546 2 GGCTTGA 30 0.0042129527 59.534542 1 TCCACGC 30 0.0042129527 59.534542 3 TAAGAGT 30 0.0042129527 59.534542 4 GGTCGGA 15 0.004230565 59.49848 98-99 CGACGGT 15 0.004230565 59.49848 110-111 CGCATCG 15 0.004230565 59.49848 74-75 GCCGCAT 15 0.004230565 59.49848 72-73 ATATAGA 30 0.0042230813 59.49848 6 TACGACG 15 0.004230565 59.49848 108-109 GACGGTA 15 0.004230565 59.49848 110-111 CATCGTT 15 0.004230565 59.49848 88-89 CGCCGCA 15 0.004230565 59.49848 70-71 CCAGTCG 15 0.004230565 59.49848 80-81 CCGCATC 15 0.004230565 59.49848 72-73 >>END_MODULE