FastQCFastQC Report
Thu 26 May 2016
SRR936190_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936190_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136590
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCC208.468374E-489.242314
TCGGCAG250.002053658271.3938452
CGCAGAA300.00423001559.4948732
ACGACGA150.00423454359.49487350-51
TAATCGC150.00423454359.494873106-107
GGTATCA1900.056.3635641
CGGCAGC350.007784287450.9956053
TCGGGTC350.007784287450.9956053
TCATGAC350.007784287450.9956053
GTACTCG350.007784287450.9956059
CTCTACG350.007784287450.9956052
GTACATG7950.049.3919751
GTGTTCG505.3182687E-447.5958989
TACATGG8300.046.592372
ACATGGG9050.043.3885273
CAGTGCG853.4603763E-641.996389
GTATCAA2900.036.9278532
CCTATTA650.001933748136.6122329
CTAATAT650.001933748136.6122323
CATGGGG2950.036.3019564
Produced by FastQC (version 0.11.5)