##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936190_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 136590 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.500556409693242 31.0 30.0 34.0 25.0 34.0 2 29.477106669595138 31.0 30.0 34.0 25.0 34.0 3 29.512306903872904 31.0 30.0 34.0 23.0 34.0 4 32.868906947799985 35.0 33.0 37.0 25.0 37.0 5 33.146269858701224 35.0 35.0 37.0 27.0 37.0 6 33.035529687385605 37.0 35.0 37.0 26.0 37.0 7 32.94987920052713 36.0 35.0 37.0 26.0 37.0 8 32.89469214437367 36.0 35.0 37.0 25.0 37.0 9 34.16798447909803 38.0 35.0 39.0 23.0 39.0 10-11 34.096119774507656 38.0 34.5 39.0 24.0 39.0 12-13 34.13468043048539 38.0 35.0 39.0 24.0 39.0 14-15 35.265670986162974 39.0 35.0 41.0 25.0 41.0 16-17 35.25974449081192 39.0 35.0 41.0 25.0 41.0 18-19 35.30517973497328 39.0 35.0 41.0 25.0 41.0 20-21 35.38305146789662 39.0 35.0 41.0 25.0 41.0 22-23 35.29803426312321 39.0 34.5 41.0 25.0 41.0 24-25 35.30284793908778 39.0 34.0 41.0 25.5 41.0 26-27 35.383834834175275 39.0 35.0 41.0 27.0 41.0 28-29 35.252189032872096 39.0 34.0 41.0 26.0 41.0 30-31 35.144823925616805 39.0 34.0 41.0 26.0 41.0 32-33 34.94267149864558 39.0 34.0 41.0 25.0 41.0 34-35 34.894721429094375 39.0 34.0 41.0 25.0 41.0 36-37 34.762149498499156 39.0 34.0 40.0 24.5 41.0 38-39 34.495296141738045 38.0 33.0 40.0 24.0 41.0 40-41 34.57595724430779 38.5 33.5 40.0 24.0 41.0 42-43 34.48536496083169 39.0 33.0 40.5 23.5 41.0 44-45 34.34517534226518 38.0 33.0 40.0 23.0 41.0 46-47 34.29846255216341 38.0 33.0 40.0 23.0 41.0 48-49 34.19751445933085 38.0 33.0 40.0 23.0 41.0 50-51 34.043722088000585 38.0 33.0 40.0 22.0 41.0 52-53 33.87426605168753 38.0 33.0 40.0 21.5 41.0 54-55 33.69129145618274 38.0 33.0 40.0 20.0 41.0 56-57 33.483487078116994 38.0 32.5 40.0 19.5 41.0 58-59 33.24374039095102 37.0 32.0 40.0 18.0 41.0 60-61 32.97232227835127 37.0 32.0 40.0 16.5 41.0 62-63 32.71887034189912 36.5 31.5 40.0 13.5 41.0 64-65 32.42307635990922 36.0 31.0 39.5 12.5 41.0 66-67 32.13636430192547 36.0 31.0 39.0 9.5 41.0 68-69 31.83561022036752 35.0 31.0 39.0 8.0 40.5 70-71 31.48322351563072 35.0 31.0 38.5 7.0 40.0 72-73 31.150497840251848 35.0 30.5 37.5 4.0 40.0 74-75 30.805168753203017 35.0 30.0 37.0 2.0 39.0 76-77 30.48473899992679 35.0 30.0 36.5 2.0 39.0 78-79 30.15194743392635 34.0 30.0 36.0 2.0 39.0 80-81 29.84548283183249 34.0 30.0 36.0 2.0 37.5 82-83 29.57463577128633 34.0 29.5 35.0 2.0 37.0 84-85 29.62594626253752 34.0 30.0 35.0 2.0 37.0 86-87 29.51570393147375 34.0 30.0 35.0 2.0 36.0 88-89 29.350274544256536 34.0 30.0 35.0 2.0 36.0 90-91 29.197411962808403 34.0 30.0 35.0 2.0 36.0 92-93 29.0807855626327 34.0 30.0 35.0 2.0 35.5 94-95 28.948590672816458 34.0 29.0 35.0 2.0 35.0 96-97 28.859934841496447 34.0 29.0 35.0 2.0 35.0 98-99 28.75582033823852 34.0 29.0 35.0 2.0 35.0 100-101 28.665356175415475 34.0 29.0 35.0 2.0 35.0 102-103 27.69233106376748 32.5 27.5 34.5 2.0 35.0 104-105 28.331385899406982 34.0 29.0 35.0 2.0 35.0 106-107 28.46041803938795 34.0 29.0 35.0 2.0 35.0 108-109 28.407507870268688 34.0 29.0 35.0 2.0 35.0 110-111 28.30632183908046 34.0 29.0 35.0 2.0 35.0 112-113 28.235050150084195 34.0 29.0 35.0 2.0 35.0 114-115 28.125671718280984 34.0 29.0 35.0 2.0 35.0 116-117 27.97374624789516 34.0 29.0 35.0 2.0 35.0 118-119 27.775715645362034 34.0 29.0 35.0 2.0 35.0 120-121 27.560677941284133 34.0 28.0 35.0 2.0 35.0 122-123 27.228640456841642 34.0 27.0 35.0 2.0 35.0 124-125 25.220012446006294 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4003323558653875 1101 2 0.491712744076505 1101 3 0.6668601316589822 1101 4 0.2695470668014508 1101 5 0.26430993999134245 1101 6 0.38554445311434193 1101 7 0.38415320378139484 1101 8 0.5361892681353311 1101 9 0.7253514314124985 1101 10-11 0.7098909184148781 1101 12-13 0.6243848780713321 1101 14-15 0.8328160210051294 1101 16-17 0.39875302623163833 1101 18-19 0.8066614627248185 1101 20-21 0.6589711693991802 1101 22-23 0.8432975254903567 1101 24-25 1.027643347839529 1101 26-27 1.0971250605815612 1101 28-29 1.1182961294113056 1101 30-31 1.2178628694241667 1101 32-33 1.581328078417883 1101 34-35 1.5414394369512152 1101 36-37 1.0379267966154444 1101 38-39 0.904662313050288 1101 40-41 0.9996896418923669 1101 42-43 0.892970095109952 1101 44-45 0.8945079695525919 1101 46-47 0.5551309981982087 1101 48-49 0.7529397537078566 1101 50-51 0.5090634877687634 1101 52-53 0.5716377916266779 1101 54-55 0.762126813496792 1101 56-57 0.9415598733519488 1101 58-59 0.6074189497859948 1101 60-61 0.7497514816489286 1101 62-63 0.45996369004767246 1101 64-65 0.9232563383768309 1101 66-67 0.42485528236665004 1101 68-69 0.5212915243559522 1101 70-71 0.6191291326770667 1101 72-73 0.8961411389949383 1101 74-75 0.8973210438900416 1101 76-77 0.7630218564035793 1101 78-79 0.9625180883068722 1101 80-81 0.8185774428607537 1101 82-83 0.2955927593211243 1101 84-85 0.5716589817363911 1101 86-87 0.6142690657548471 1101 88-89 0.5856848003706006 1101 90-91 0.21625373566087092 1101 92-93 0.014609662879944807 1101 94-95 0.47417641272880573 1101 96-97 0.4904168868872816 1101 98-99 0.48833083128673493 1101 100-101 0.38070006402447376 1101 102-103 0.45353371664553777 1101 104-105 0.47476598939294234 1101 106-107 0.1499798649843349 1101 108-109 0.03957849353356124 1101 110-111 0.26509981720599995 1101 112-113 0.5583139847228686 1101 114-115 0.6083035062827804 1101 116-117 0.5935741747811072 1101 118-119 0.610686867850994 1101 120-121 0.7155691557657775 1101 122-123 0.3552986794101791 1101 124-125 0.3378497446361166 1102 1 0.7819438436994517 1102 2 0.9757574189867562 1102 3 0.7604381133103573 1102 4 0.5425825673997764 1102 5 0.7683785478892275 1102 6 0.7903310506412708 1102 7 1.0420311455444633 1102 8 0.5537800140467724 1102 9 0.9086382283809868 1102 10-11 1.2851601645257702 1102 12-13 1.1742851572895106 1102 14-15 1.066461812788134 1102 16-17 0.7875045220433563 1102 18-19 0.8162944902477562 1102 20-21 0.8642364266787936 1102 22-23 1.2581359778238195 1102 24-25 1.1622664032842778 1102 26-27 0.8191434092054095 1102 28-29 1.1396363767188475 1102 30-31 1.1917160804333378 1102 32-33 1.518424209251549 1102 34-35 1.3916588226711966 1102 36-37 1.1000528436836632 1102 38-39 1.3134576860060108 1102 40-41 0.6994104237032985 1102 42-43 0.9352118182850617 1102 44-45 0.8434509292653942 1102 46-47 0.9635474321830557 1102 48-49 0.7886796819088957 1102 50-51 0.6394184937520109 1102 52-53 0.7214981825321374 1102 54-55 1.0152381018892953 1102 56-57 0.5383887524903628 1102 58-59 0.8302948971334132 1102 60-61 0.5984152231726512 1102 62-63 0.5933900961106957 1102 64-65 0.8551869327126838 1102 66-67 0.9590595105277941 1102 68-69 0.8197957126287889 1102 70-71 0.5427430138098899 1102 72-73 0.27962737752704925 1102 74-75 0.30382283886411443 1102 76-77 0.5577167108112384 1102 78-79 0.6768379925749244 1102 80-81 0.9254382326493449 1102 82-83 0.961488251941784 1102 84-85 0.8093334931045604 1102 86-87 1.2025020135011601 1102 88-89 1.3443405642317892 1102 90-91 1.1906055505791002 1102 92-93 1.2114983744874444 1102 94-95 1.0203870230199925 1102 96-97 0.8838353152877616 1102 98-99 0.9635601890849017 1102 100-101 1.4612545115713438 1102 102-103 1.1162780325609702 1102 104-105 1.2325043220614695 1102 106-107 0.8904864465559363 1102 108-109 0.9762757412399807 1102 110-111 1.0570423780356784 1102 112-113 1.2114651614280945 1102 114-115 0.9255751456924344 1102 116-117 1.0902235565122602 1102 118-119 1.1966262376156536 1102 120-121 1.3542408749520582 1102 122-123 1.1087091301481138 1102 124-125 1.096086681213702 1103 1 0.3223507044892422 1103 2 0.4504283404067806 1103 3 0.46043811331035656 1103 4 0.5631250484528252 1103 5 -0.28156161964168547 1103 6 0.18829674669232332 1103 7 0.342868800111674 1103 8 0.7701342222637706 1103 9 0.5648009726969008 1103 10-11 0.6938358725433176 1103 12-13 0.5234674468786693 1103 14-15 0.5690545531152154 1103 16-17 0.1464594482499777 1103 18-19 0.586477976486286 1103 20-21 0.3080537382065103 1103 22-23 0.42586633282979847 1103 24-25 0.6996616964633802 1103 26-27 0.5351984550769728 1103 28-29 0.5242594321635963 1103 30-31 0.816486722635176 1103 32-33 0.33591523438119 1103 34-35 0.20657705163011286 1103 36-37 0.6847157874411423 1103 38-39 0.7188824965365228 1103 40-41 0.6258364308831901 1103 42-43 0.9342544987796728 1103 44-45 0.7018474190938733 1103 46-47 0.8234796220514227 1103 48-49 0.7116553500381357 1103 50-51 0.45631119419078203 1103 52-53 0.21613320447071516 1103 54-55 0.8424020428545873 1103 56-57 0.6112846439941535 1103 58-59 0.4308134451988366 1103 60-61 0.3781918486213982 1103 62-63 0.2512480937174004 1103 64-65 0.5241737695694866 1103 66-67 -0.043034625890228995 1103 68-69 0.3653282216036544 1103 70-71 0.33243188497064224 1103 72-73 0.3754989371600779 1103 74-75 0.6450274659083917 1103 76-77 0.32953561787147123 1103 78-79 0.12536212500412347 1103 80-81 0.07545817680570721 1103 82-83 -0.14706779113759438 1103 84-85 -0.029258449456271762 1103 86-87 0.11885622171815413 1103 88-89 0.2966939746825261 1103 90-91 0.46396015767922094 1103 92-93 0.45038947939371 1103 94-95 0.4581213668572488 1103 96-97 0.35968294193315486 1103 98-99 0.05484459275462683 1103 100-101 0.14904868787768422 1103 102-103 -0.04875986133611576 1103 104-105 0.08715131049386216 1103 106-107 -0.16424031850190346 1103 108-109 -0.24253159820955972 1103 110-111 -0.09040476994996283 1103 112-113 -0.024254822616580185 1103 114-115 -0.04077906252456032 1103 116-117 -0.1105542655858649 1103 118-119 -0.5315149670113897 1103 120-121 -0.6330546974452282 1103 122-123 -0.6309398526999139 1103 124-125 -0.09563649389599149 1104 1 0.5801080949071817 1104 2 0.6614579020785456 1104 3 0.5359936688659133 1104 4 0.183838606047118 1104 5 0.3222915913674882 1104 6 0.36839868349150606 1104 7 0.7257026431290043 1104 8 0.41223412032697127 1104 9 -0.1605405155803652 1104 10-11 0.41366257998470957 1104 12-13 0.6796957853088443 1104 14-15 0.6326936968461041 1104 16-17 0.4964900292897383 1104 18-19 0.30740560135886597 1104 20-21 0.7543330450362831 1104 22-23 0.11214563965957325 1104 24-25 0.5768074660862155 1104 26-27 0.2835878536498555 1104 28-29 0.48731753613913753 1104 30-31 0.5506049693222366 1104 32-33 0.5712261416186948 1104 34-35 0.5095332188064674 1104 36-37 0.4603426987561221 1104 38-39 0.23046251692388608 1104 40-41 0.18989351549073774 1104 42-43 0.5637142337440011 1104 44-45 0.6448035862702213 1104 46-47 0.6486682051299084 1104 48-49 0.9935105997832991 1104 50-51 0.6264233246698794 1104 52-53 0.7172952839814215 1104 54-55 0.5750931743530572 1104 56-57 0.7502245012826307 1104 58-59 0.827541273945009 1104 60-61 0.4469176386315894 1104 62-63 -0.0438079715221491 1104 64-65 0.17025939648080168 1104 66-67 0.5498807665664458 1104 68-69 0.13984402180752653 1104 70-71 0.01950629883404531 1104 72-73 0.6207384886381639 1104 74-75 0.41304989200421005 1104 76-77 0.45245101032814716 1104 78-79 0.32698292011115626 1104 80-81 -0.07881297508012253 1104 82-83 0.16970081232825862 1104 84-85 -0.21124621704036883 1104 86-87 0.11428945311468652 1104 88-89 0.0438091632656068 1104 90-91 0.027996854926925607 1104 92-93 0.10608774646812336 1104 94-95 0.3037686655320684 1104 96-97 0.014270097896456946 1104 98-99 0.2280046335293484 1104 100-101 0.14743808645056689 1104 102-103 0.32502199391363007 1104 104-105 0.3197506988730652 1104 106-107 0.08691156732887961 1104 108-109 0.07849796346220472 1104 110-111 0.23622112199702983 1104 112-113 0.2120448715730241 1104 114-115 0.027121039412236314 1104 116-117 0.11602065796153482 1104 118-119 -0.08380854499304391 1104 120-121 0.23955488461389507 1104 122-123 0.17344342966502424 1104 124-125 0.11627991792867931 1105 1 0.5093955886789807 1105 2 0.9586975632643941 1105 3 1.0299448397677544 1105 4 1.0232805592110452 1105 5 0.8243543716365522 1105 6 0.775493850407301 1105 7 0.592508821504687 1105 8 0.8767084601376283 1105 9 0.7678700474490299 1105 10-11 0.7702644733578978 1105 12-13 0.9272194524737642 1105 14-15 0.6882691826458043 1105 16-17 0.5620804627725491 1105 18-19 0.5153976292612015 1105 20-21 0.22289308489676785 1105 22-23 -0.08754544155617339 1105 24-25 0.2177840480493387 1105 26-27 0.5774393733309751 1105 28-29 0.9271680593080447 1105 30-31 1.056962717204641 1105 32-33 0.9324717818777941 1105 34-35 0.9662347634003865 1105 36-37 0.7744533116111327 1105 38-39 0.9930833440290172 1105 40-41 0.8568192753312971 1105 42-43 0.6969977414669657 1105 44-45 0.8875140994740107 1105 46-47 1.1263861921752536 1105 48-49 0.6905708887718376 1105 50-51 0.41971679751492985 1105 52-53 0.644260904310265 1105 54-55 0.9316253118717412 1105 56-57 0.9183361106498467 1105 58-59 0.7990509899390332 1105 60-61 0.7960755858164461 1105 62-63 0.854747085777305 1105 64-65 0.43342830529994103 1105 66-67 0.8295020919276794 1105 68-69 0.6691913063117596 1105 70-71 0.9233727661982769 1105 72-73 1.148013027751265 1105 74-75 0.9576637435238915 1105 76-77 0.7413100038508169 1105 78-79 1.0310785852830548 1105 80-81 0.9306439257669084 1105 82-83 1.4098602542814191 1105 84-85 1.18519623437966 1105 86-87 1.1021120739417896 1105 88-89 0.845383652553096 1105 90-91 1.0456650163619017 1105 92-93 1.1029586981372788 1105 94-95 1.1018453969720277 1105 96-97 1.378844088927849 1105 98-99 1.241138664421225 1105 100-101 1.2103479020958083 1105 102-103 1.1411854219156226 1105 104-105 1.2691976345980294 1105 106-107 0.9661542180513543 1105 108-109 1.0202219295807922 1105 110-111 1.2052096621066468 1105 112-113 0.9378445726193583 1105 114-115 0.8963686726957434 1105 116-117 0.9957217042993527 1105 118-119 0.9807654460383475 1105 120-121 0.8209400365820052 1105 122-123 0.44588986713388223 1105 124-125 0.14613542365862386 1106 1 -0.9764954955828706 1106 2 -1.707838555951536 1106 3 -1.5788631967333124 1106 4 -1.3025854599402678 1106 5 -1.1184070985888397 1106 6 -1.516162209278992 1106 7 -1.5869417042751692 1106 8 -0.9290565152868098 1106 9 -1.3136894723974493 1106 10-11 -1.4751777887683204 1106 12-13 -1.6364420118769942 1106 14-15 -1.6502175113052786 1106 16-17 -1.522083478716084 1106 18-19 -1.5780286538570465 1106 20-21 -1.4617271199321777 1106 22-23 -1.4290673637368414 1106 24-25 -5.306928584374727 1106 26-27 -2.77343301000856 1106 28-29 -2.111692653089399 1106 30-31 -2.0796812994793186 1106 32-33 -6.916178031112985 1106 34-35 -2.3537370383502534 1106 36-37 -1.4199775341405285 1106 38-39 -1.6172618887044479 1106 40-41 -2.220363641229305 1106 42-43 -2.123816091340913 1106 44-45 -1.9938378499257965 1106 46-47 -1.7795511058841598 1106 48-49 -1.4208513604301984 1106 50-51 -1.3640085045392354 1106 52-53 -1.8435198543009719 1106 54-55 -2.0756200716440283 1106 56-57 -2.7453116462185783 1106 58-59 -1.5667527580782803 1106 60-61 -1.8444554812131422 1106 62-63 -1.8156662927254494 1106 64-65 -1.9056066879442355 1106 66-67 -1.6147965745301072 1106 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0.15015945198615555 2216 28-29 0.041577785693498015 2216 30-31 0.06364121412318013 2216 32-33 0.5396634559383671 2216 34-35 -0.041031249405058645 2216 36-37 0.27791845633188217 2216 38-39 0.6140156957711795 2216 40-41 0.4667087264235974 2216 42-43 0.2520683632745957 2216 44-45 0.5825813640480035 2216 46-47 0.04755115224815398 2216 48-49 0.16166270911187297 2216 50-51 0.48569842865087054 2216 52-53 -0.22092218482428194 2216 54-55 -0.28786582742947076 2216 56-57 0.06727738304140018 2216 58-59 -0.4169150540407287 2216 60-61 -0.36387261686454053 2216 62-63 -1.184568518165051 2216 64-65 -0.4471381079755261 2216 66-67 -0.5289665299279065 2216 68-69 -0.23264558009383762 2216 70-71 -0.45812887644818545 2216 72-73 -0.5019234246119844 2216 74-75 -0.5306458893386292 2216 76-77 0.07221333950224107 2216 78-79 0.020851012802779678 2216 80-81 -0.4153429810218938 2216 82-83 -0.22305022748160752 2216 84-85 -0.18864372149669606 2216 86-87 0.07367150896732966 2216 88-89 -0.09377847785145121 2216 90-91 0.16093803139751373 2216 92-93 0.18492315942118154 2216 94-95 0.11366765543105828 2216 96-97 -0.006050757718515598 2216 98-99 0.33842649924532964 2216 100-101 0.08352840136441486 2216 102-103 -0.3750611908754351 2216 104-105 -0.1465238109308551 2216 106-107 -0.20265468341384008 2216 108-109 -0.25260126410761075 2216 110-111 -0.7017230253588806 2216 112-113 -0.4926966614038051 2216 114-115 0.011090142204871256 2216 116-117 -0.5749240835714389 2216 118-119 -0.44958394606256036 2216 120-121 -0.06884677908188763 2216 122-123 -0.17759638019832025 2216 124-125 -0.06555014743079823 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4702.0 3 648.0 4 498.0 5 462.0 6 756.0 7 748.0 8 654.0 9 704.0 10 710.0 11 781.0 12 776.0 13 798.0 14 785.0 15 735.0 16 679.0 17 591.0 18 545.0 19 507.0 20 468.0 21 529.0 22 654.0 23 774.0 24 1041.0 25 1589.0 26 1924.0 27 1921.0 28 2311.0 29 2854.0 30 3513.0 31 4244.0 32 5383.0 33 7237.0 34 10099.0 35 14321.0 36 23050.0 37 29467.0 38 9111.0 39 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.71190945098068 18.33090512413152 9.153006464649422 22.80417896023838 2 20.369868730278426 21.076367789499887 35.50212681841144 23.05163666181025 3 17.45588988945018 27.988139688117723 30.75554579398199 23.800424628450106 4 11.837789556915688 16.602483380677658 35.610741792836855 35.9489852695698 5 11.970392497089767 41.82608886643678 33.632776179284996 12.570742457188459 6 31.515945763903126 37.25876358098808 17.336808504407415 13.888482150701378 7 26.89987407385715 35.58511728702375 21.20464462471081 16.310364014408293 8 26.844428825583694 33.881701772496655 19.70758564138608 19.56628376053358 9 26.98573070643619 14.22170484745986 18.695044184292794 40.097520261811155 10-11 24.01171410268143 27.175253958085477 28.67905188981422 20.13398004941887 12-13 24.76406978702219 26.268605357757323 28.01364697958078 20.953677875639702 14-15 23.15776757670935 28.063432098223114 23.870866187850968 24.90793413721657 16-17 22.949841492967852 29.81140225643729 25.37283928924422 21.86591696135064 18-19 21.935835767679208 28.6070416657515 27.061140518497368 22.395982048071925 20-21 23.58057501812032 27.582419996046475 26.691412799168297 22.14559218666491 22-23 23.333076841337714 27.915123134478755 26.401453943198018 22.350346080985513 24-25 22.841119579462173 28.406802990035658 26.432603395637944 22.319474034864225 26-27 22.63412598471315 28.018200720414676 26.491346238322542 22.856327056549624 28-29 22.85705918528714 28.045655548071576 26.50049784754151 22.596787419099776 30-31 22.708803115939908 27.950844876563096 26.783831668960673 22.55652033853633 32-33 22.108253347683164 27.999736431724838 27.23392416555016 22.658086055041842 34-35 23.18862861807546 28.62759309897978 26.324690585224964 21.859087697719794 36-37 22.831633586892064 28.19407126488956 26.246257019232882 22.7280381289855 38-39 22.34648119337421 28.008053445593482 26.826027271895303 22.819438089137 40-41 23.049609043254165 27.994772600814127 27.052522915629485 21.903095440302224 42-43 22.654791451602275 28.04695908102528 26.849187697218625 22.449061770153822 44-45 22.629166758183427 28.25195662837605 26.920570771742554 22.198305841697966 46-47 22.647104043576622 28.82778009620242 26.03359031240162 22.49152554781934 48-49 22.415257339483123 28.900358737828537 25.996412621714622 22.687971300973718 50-51 22.53455647641081 28.250651594576393 26.44009722669634 22.774694702316456 52-53 22.81744070694092 27.89327066334281 26.410349333948318 22.878939295767946 54-55 22.57226110639295 28.189884909362462 26.363223709257035 22.874630274987553 56-57 22.65865229741998 28.234910826719773 26.17287023750256 22.93356663835769 58-59 22.89924041365425 27.845154205179828 25.888532991672005 23.367072389493913 60-61 22.372471858698635 27.946554406515972 26.635673103322045 23.04530063146335 62-63 22.390327115119923 27.447140305151258 26.672548100858055 23.489984478870767 64-65 22.75830511576828 27.79353894023977 26.32268692230255 23.125469021689394 66-67 22.243291028959035 28.21849410093748 26.14586040654662 23.39235446355686 68-69 23.14746009027048 27.223908133494888 26.334015425783097 23.294616350451534 70-71 22.753462968928456 27.3256069347234 26.45437373707793 23.466556359270214 72-73 23.104997089820227 27.740053225174705 26.34719359833662 22.807756086668448 74-75 22.445520669748916 27.691935997540057 26.555676356355036 23.306866976355987 76-77 22.55672125866651 27.691834627971506 26.46259947726391 23.288844636098073 78-79 22.546672523610805 28.04378065744198 26.57734826854089 22.83219855040633 80-81 22.483225161708344 27.52867188672546 27.180912009429786 22.80719094213642 82-83 22.552895526758913 27.751299509480926 26.07108865949191 23.624716304268247 84-85 22.2015601492062 28.052668762972267 26.79415328411042 22.95161780371112 86-87 22.398216547452577 27.74784206634502 26.468822525972076 23.385118860230325 88-89 22.142420481958585 27.734399308873 26.72406534957189 23.399114859596526 90-91 22.148196414059697 27.866446053488936 27.05744972142706 22.927907811024316 92-93 22.58527407038634 27.756261485185483 26.87771343226761 22.780751012160568 94-95 22.18699895306357 27.767609397535676 26.673817071652916 23.371574577747843 96-97 22.410012555961885 28.04591894632418 26.633281718446284 22.910786779267656 98-99 22.064368287343783 28.071074537481056 26.211847220493596 23.652709954681566 100-101 22.01303906962102 28.47620058642868 26.4149147628478 23.095845581102502 102-103 22.34326839303887 28.558720815304532 26.25725727924327 22.840753512413333 104-105 22.153687339390434 28.504125515231827 26.44246608438454 22.899721060993198 106-107 21.679716813640972 28.906899189902518 26.459035716769712 22.954348279686794 108-109 21.69114417079099 29.23316836032448 26.090139690163117 22.98554777872141 110-111 21.939050059485677 28.569232177175802 26.123181111009426 23.368536652329094 112-113 22.29506876493995 29.09395739758472 26.007680002342802 22.603293835132533 114-115 21.43591883650527 29.12177818776837 26.502597217188857 22.939705758537503 116-117 21.6233431072162 28.827097449638877 26.075401662658276 23.474157780486642 118-119 21.790873216338305 28.808744609662707 26.1305980803444 23.269784093654593 120-121 21.83785604205332 28.514845686130236 25.990475039992383 23.656823231824056 122-123 21.766919495124025 28.40183617887369 26.332474302281312 23.498770023720972 124-125 21.840338533849724 28.35184385272606 26.20562417178543 23.602193441638786 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13.0 1 15.0 2 15.5 3 15.0 4 21.0 5 25.5 6 20.5 7 17.5 8 26.0 9 34.0 10 32.0 11 25.5 12 28.5 13 38.5 14 46.0 15 50.5 16 62.0 17 69.0 18 78.5 19 94.0 20 128.5 21 213.0 22 289.0 23 360.5 24 418.0 25 490.5 26 630.0 27 853.5 28 1033.5 29 1162.0 30 1564.0 31 1974.5 32 2142.0 33 2564.5 34 3112.5 35 3242.0 36 3363.0 37 3764.5 38 4009.0 39 3886.0 40 3752.0 41 3914.5 42 4303.0 43 4688.5 44 4856.5 45 4894.0 46 4902.5 47 5107.0 48 5563.5 49 5587.5 50 5446.0 51 5220.0 52 4614.0 53 4394.5 54 4250.0 55 4104.5 56 4111.0 57 3675.0 58 3233.5 59 2800.5 60 2297.0 61 1883.0 62 1509.0 63 1140.5 64 820.0 65 690.5 66 541.0 67 395.0 68 303.5 69 242.0 70 222.0 71 200.0 72 217.0 73 211.5 74 152.5 75 133.5 76 90.0 77 51.0 78 33.0 79 20.5 80 15.5 81 21.0 82 17.0 83 9.5 84 6.5 85 5.5 86 5.5 87 6.0 88 4.0 89 0.5 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.321180174244088E-4 2 7.321180174244088E-4 3 0.0 4 0.0014642360348488177 5 0.0021963540522732267 6 0.0014642360348488177 7 0.0014642360348488177 8 0.0021963540522732267 9 0.0021963540522732267 10-11 0.001830295043561022 12-13 0.0021963540522732267 14-15 0.0021963540522732267 16-17 0.0021963540522732267 18-19 0.0021963540522732267 20-21 0.0021963540522732267 22-23 0.09773775532615858 24-25 0.0021963540522732267 26-27 0.0014642360348488177 28-29 0.0014642360348488177 30-31 0.0014642360348488177 32-33 0.0021963540522732267 34-35 0.0010981770261366134 36-37 7.321180174244088E-4 38-39 0.001830295043561022 40-41 0.0014642360348488177 42-43 0.0021963540522732267 44-45 0.0021963540522732267 46-47 0.0021963540522732267 48-49 0.0 50-51 0.0014642360348488177 52-53 0.0010981770261366134 54-55 0.0014642360348488177 56-57 0.0014642360348488177 58-59 0.001830295043561022 60-61 0.001830295043561022 62-63 0.0014642360348488177 64-65 0.001830295043561022 66-67 3.660590087122044E-4 68-69 3.660590087122044E-4 70-71 0.0014642360348488177 72-73 3.660590087122044E-4 74-75 0.0010981770261366134 76-77 7.321180174244088E-4 78-79 0.0 80-81 0.0010981770261366134 82-83 0.0 84-85 3.660590087122044E-4 86-87 7.321180174244088E-4 88-89 0.0010981770261366134 90-91 7.321180174244088E-4 92-93 7.321180174244088E-4 94-95 7.321180174244088E-4 96-97 0.0010981770261366134 98-99 7.321180174244088E-4 100-101 3.660590087122044E-4 102-103 0.0021963540522732267 104-105 7.321180174244088E-4 106-107 0.0010981770261366134 108-109 0.0014642360348488177 110-111 0.001830295043561022 112-113 0.0010981770261366134 114-115 0.0010981770261366134 116-117 0.0010981770261366134 118-119 0.0021963540522732267 120-121 0.0010981770261366134 122-123 0.0014642360348488177 124-125 7.321180174244088E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 136590.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.15557507870269 #Duplication Level Percentage of deduplicated Percentage of total 1 57.92498802557321 20.363862654659933 2 11.714113163539434 8.236327696024599 3 8.134280180761781 8.578958928179222 4 5.772714966992233 8.117724577201844 5 3.8922093338053685 6.841642872831101 6 2.8613673754138986 6.035580935646826 7 2.057518898769237 5.063328208507211 8 1.4889939398987901 4.187715059667618 9 1.107894791644974 3.505381067428069 >10 4.970948999354422 27.473460721868364 >50 0.07288781523980091 1.518412768138224 >100 0.002082509006851455 0.07760450984698734 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.321180174244088E-4 0.0 16-17 0.0 0.0 0.0 7.321180174244088E-4 0.0 18-19 0.0 0.0 0.0 7.321180174244088E-4 0.0 20-21 0.0 0.0 0.0 7.321180174244088E-4 0.0 22-23 0.0 0.0 0.0 0.0014642360348488177 0.0 24-25 0.0 0.0 0.0 0.0025624130609854306 0.0 26-27 0.0 0.0 0.0 0.0029284720696976354 0.0 28-29 0.0 0.0 0.0 0.005124826121970861 0.0 30-31 0.0 0.0 0.0 0.006589062156819679 0.0 32-33 0.0 0.0 0.0 0.011713888278790541 0.0 34-35 0.0 0.0 0.0 0.020499304487883445 0.0 36-37 0.0 0.0 0.0 0.04063254996705469 0.0 38-39 0.0 0.0 0.0 0.07394391975986529 0.0 40-41 0.0 0.0 0.0 0.10579105351782708 0.0 42-43 0.0 0.0 0.0 0.1347097152060912 0.0 44-45 0.0 0.0 0.0 0.1833955633648144 0.0 46-47 0.0 0.0 0.0 0.22805476242770334 0.0 48-49 0.0 0.0 0.0 0.28515996778680724 0.0 50-51 0.0 0.0 0.0 0.34922029431144297 0.0 52-53 0.0 0.0 0.0 0.4121824438099422 0.0 54-55 0.0 0.0 0.0 0.4788051833955633 0.0 56-57 0.0 0.0 0.0 0.5816677648436928 0.0 58-59 0.0 0.0 0.0 0.6870927593528077 0.0 60-61 0.0 0.0 0.0 0.7954462259316202 0.0 62-63 0.0 0.0 0.0 0.9100226956585402 0.0 64-65 0.0 0.0 0.0 1.0608390072479685 0.0 66-67 0.0 0.0 0.0 1.2039680796544403 0.0 68-69 0.0 0.0 0.0 1.3811406398711472 0.0 70-71 0.0 0.0 0.0 1.577714327549601 0.0 72-73 0.0 0.0 0.0 1.770993484149645 0.0 74-75 0.0 0.0 0.0 1.9737901749762061 0.0 76-77 0.0 0.0 0.0 2.2208800058569445 0.0 78-79 0.0 0.0 0.0 2.5115308587744343 0.0 80-81 0.0 0.0 0.0 2.846108792737389 0.0 82-83 0.0 0.0 0.0 3.156526832125339 0.0 84-85 0.0 0.0 0.0 3.4501061571125264 0.0 86-87 0.0 0.0 0.0 3.811406398711472 0.0 88-89 0.0 0.0 0.0 4.23603484881763 0.0 90-91 0.0 0.0 0.0 4.699099494838569 0.0 92-93 0.0 0.0 0.0 5.133245479171242 0.0 94-95 0.0 0.0 0.0 5.588256827000512 0.0 96-97 0.0 0.0 0.0 6.086097078849111 0.0 98-99 0.0 0.0 0.0 6.665568489640529 0.0 100-101 0.0 0.0 0.0 7.304341459843327 0.0 102-103 0.0 0.0 0.0 7.885277106669595 0.0 104-105 0.0 0.0 0.0 8.559557800717474 0.0 106-107 0.0 0.0 0.0 9.24298996998316 0.0 108-109 0.0 0.0 0.0 9.932279083388242 0.0 110-111 0.0 0.0 0.0 10.710154476901677 0.0 112-113 0.0 0.0 0.0 11.476682041145033 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTCC 20 8.468374E-4 89.24231 4 TCGGCAG 25 0.0020536582 71.393845 2 CGCAGAA 30 0.004230015 59.494873 2 ACGACGA 15 0.004234543 59.494873 50-51 TAATCGC 15 0.004234543 59.494873 106-107 GGTATCA 190 0.0 56.363564 1 CGGCAGC 35 0.0077842874 50.995605 3 TCGGGTC 35 0.0077842874 50.995605 3 TCATGAC 35 0.0077842874 50.995605 3 GTACTCG 35 0.0077842874 50.995605 9 CTCTACG 35 0.0077842874 50.995605 2 GTACATG 795 0.0 49.391975 1 GTGTTCG 50 5.3182687E-4 47.595898 9 TACATGG 830 0.0 46.59237 2 ACATGGG 905 0.0 43.388527 3 CAGTGCG 85 3.4603763E-6 41.99638 9 GTATCAA 290 0.0 36.927853 2 CCTATTA 65 0.0019337481 36.612232 9 CTAATAT 65 0.0019337481 36.612232 3 CATGGGG 295 0.0 36.301956 4 >>END_MODULE