FastQCFastQC Report
Thu 26 May 2016
SRR936190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936190_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136590
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5490.4019327915660004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.2657588403250604No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.2225638772970203No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG208.4493234E-489.292882
GGTATCA4450.078.954471
GGGTATA300.00421440859.5504041
GGCCGGT300.00422053259.5285842
ATAGAGC300.00422053259.5285845
CGGTACA300.00422053259.5285845
AACGTCA150.004237330559.4849928-29
GTATCAA6500.054.0336382
TAGGTAA350.007766864351.0244985
GCCGGTA350.007766864351.0244983
CCGGTAC350.007766864351.0244984
ACGCCTA255.322429E-447.6054250-51
TAGTAGC505.3226284E-447.5879866
ATCAACG8850.041.0310024
TTACCTA300.001308384239.6566662-63
AACGCAG9200.039.4411357
TCAACGC9250.039.2566875
CAACGCA9250.039.227946
TATCAAC9450.039.055793
CATAGAA650.001928372936.6329772
Produced by FastQC (version 0.11.5)