##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936190_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 136590 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.355706859945823 31.0 31.0 34.0 30.0 34.0 2 31.334790248188007 31.0 31.0 34.0 28.0 34.0 3 31.66788198257559 31.0 31.0 34.0 30.0 34.0 4 35.51496449227616 37.0 35.0 37.0 33.0 37.0 5 35.142294457866605 37.0 35.0 37.0 33.0 37.0 6 35.339007247968375 37.0 35.0 37.0 33.0 37.0 7 35.16092686141006 37.0 35.0 37.0 32.0 37.0 8 35.2554579398199 37.0 35.0 37.0 32.0 37.0 9 36.91179442126071 39.0 37.0 39.0 33.0 39.0 10-11 36.79352441613588 39.0 37.0 39.0 32.0 39.0 12-13 36.74625155575079 39.0 37.0 39.0 32.0 39.0 14-15 37.85001830295043 40.0 37.0 41.0 32.0 41.0 16-17 37.74961563804085 40.0 37.0 41.0 32.0 41.0 18-19 37.85563364814408 40.0 37.0 41.0 32.0 41.0 20-21 37.901013983454135 40.0 37.0 41.0 32.0 41.0 22-23 37.825309319862356 40.0 37.0 41.0 32.0 41.0 24-25 37.798001317812435 40.0 37.0 41.0 32.0 41.0 26-27 37.59256534153305 40.0 36.5 41.0 32.0 41.0 28-29 37.434047148400325 40.0 36.0 41.0 32.0 41.0 30-31 37.246295482831826 39.0 36.0 41.0 31.0 41.0 32-33 37.02568270005125 39.0 35.5 41.0 31.0 41.0 34-35 36.823610806061936 39.0 35.0 41.0 30.5 41.0 36-37 36.585720038070136 39.0 35.0 41.0 30.0 41.0 38-39 36.54316933889743 39.0 35.0 41.0 30.0 41.0 40-41 36.53602020645728 39.0 35.0 41.0 30.0 41.0 42-43 36.394739732044805 39.0 35.0 41.0 30.0 41.0 44-45 36.180975913317226 39.0 35.0 41.0 30.0 41.0 46-47 35.85685994582327 39.0 35.0 41.0 28.5 41.0 48-49 35.5672816458013 39.0 35.0 41.0 27.0 41.0 50-51 35.357646972691995 39.0 35.0 41.0 26.5 41.0 52-53 35.17119481660444 39.0 35.0 41.0 26.0 41.0 54-55 34.98650340434878 38.5 34.0 40.5 26.0 41.0 56-57 34.73749542426239 38.0 34.0 40.0 25.0 41.0 58-59 34.5090672816458 38.0 34.0 40.0 25.0 41.0 60-61 34.26478878395197 37.5 34.0 40.0 24.5 41.0 62-63 34.04683725016473 37.0 34.0 40.0 24.0 41.0 64-65 33.801010322864045 37.0 33.0 40.0 24.0 41.0 66-67 33.536364301925474 36.0 33.0 39.0 23.5 41.0 68-69 33.20919906288894 36.0 33.0 39.0 23.0 41.0 70-71 32.86561973790175 35.0 32.5 39.0 22.5 40.5 72-73 32.58938428874735 35.0 32.0 38.0 22.5 40.0 74-75 32.430910022695656 35.0 32.0 37.0 23.0 39.5 76-77 32.14684091075482 35.0 32.0 37.0 22.0 39.0 78-79 31.851317812431365 35.0 32.0 36.0 22.0 39.0 80-81 31.605575078702685 35.0 32.0 36.0 22.0 37.5 82-83 31.362548502818655 35.0 32.0 35.5 21.0 37.0 84-85 31.13335163628377 35.0 32.0 35.0 20.0 37.0 86-87 30.98772604143788 35.0 32.0 35.0 20.0 36.0 88-89 30.850248920125924 35.0 32.0 35.0 20.0 36.0 90-91 30.727417819752546 35.0 32.0 35.0 20.0 36.0 92-93 30.625228786880445 34.5 32.0 35.0 20.0 35.5 94-95 30.506999048246577 34.0 31.0 35.0 19.0 35.0 96-97 30.450849256900213 34.0 31.0 35.0 19.0 35.0 98-99 30.382886741342702 34.0 31.0 35.0 18.0 35.0 100-101 30.312830368255362 34.0 31.0 35.0 18.0 35.0 102-103 29.282681748297826 33.5 29.5 34.5 16.0 35.0 104-105 30.04729116333553 34.0 31.0 35.0 17.0 35.0 106-107 30.212164140859507 34.0 31.0 35.0 17.0 35.0 108-109 30.190921736583938 34.0 31.0 35.0 17.0 35.0 110-111 30.171154550113478 34.0 31.0 35.0 15.0 35.0 112-113 30.09379529980233 34.0 31.0 35.0 10.0 35.0 114-115 30.096365034043487 34.0 31.0 35.0 8.5 35.0 116-117 29.983871440076143 34.0 31.0 35.0 4.5 35.0 118-119 29.853620323596164 34.0 31.0 35.0 2.0 35.0 120-121 29.661402738121385 34.0 31.0 35.0 2.0 35.0 122-123 29.426319642726405 34.0 31.0 35.0 2.0 35.0 124-125 27.302383044146715 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3955653200385605 1101 2 0.7369651581726799 1101 3 0.6151098835127087 1101 4 0.2940297315605207 1101 5 0.6159389652979002 1101 6 0.5444497812858771 1101 7 0.665051628941633 1101 8 0.547591771933817 1101 9 0.4958508989583166 1101 10-11 0.6649700086500232 1101 12-13 0.43254293776674757 1101 14-15 0.7811812854679019 1101 16-17 0.8908530217273096 1101 18-19 0.9550278264689354 1101 20-21 0.8444158760595286 1101 22-23 0.7166102466566784 1101 24-25 0.8115144164584223 1101 26-27 1.0123267668777203 1101 28-29 0.7474783680001309 1101 30-31 0.834508213945611 1101 32-33 0.9283274842642157 1101 34-35 1.0156437331440955 1101 36-37 0.5792167576851028 1101 38-39 0.35682556992607317 1101 40-41 0.9402515939538105 1101 42-43 0.5646799265197231 1101 44-45 0.723953445820058 1101 46-47 0.3411487750548545 1101 48-49 0.23544881730030198 1101 50-51 0.6188692696759972 1101 52-53 0.6276420211565608 1101 54-55 0.6129604597304308 1101 56-57 0.742289242499858 1101 58-59 0.981982585845671 1101 60-61 0.9727585103973908 1101 62-63 0.884751605569285 1101 64-65 0.6272388701758587 1101 66-67 0.7527707674459094 1101 68-69 0.8829662502044187 1101 70-71 1.1195675827125484 1101 72-73 0.7443999658145515 1101 74-75 0.6520465292106792 1101 76-77 0.6135351428272955 1101 78-79 0.4398352053342691 1101 80-81 0.6638588214506775 1101 82-83 0.6676309200141901 1101 84-85 0.42148942269523104 1101 86-87 0.45563489836617777 1101 88-89 0.6227115500275637 1101 90-91 0.3926494297853935 1101 92-93 0.26696188640727314 1101 94-95 0.7407985801656132 1101 96-97 0.7271670293031178 1101 98-99 0.4116855392304828 1101 100-101 0.342855643665505 1101 102-103 0.04547954196224069 1101 104-105 0.18111723936040747 1101 106-107 0.29072509621786935 1101 108-109 0.2439105207471144 1101 110-111 0.21781457659788117 1101 112-113 0.15834920438055988 1101 114-115 0.10568629360683346 1101 116-117 0.1060216691063971 1101 118-119 0.17718923016958144 1101 120-121 0.27631045621976114 1101 122-123 -0.025828256483602985 1101 124-125 0.012972708331279392 1102 1 0.45679388007047095 1102 2 0.7903756089106153 1102 3 0.3930117582634054 1102 4 0.41640707499889373 1102 5 0.5752129980063145 1102 6 0.391717431864258 1102 7 0.5664078315742671 1102 8 0.2634274320853933 1102 9 0.42022265803290537 1102 10-11 0.6723094581913145 1102 12-13 0.8276765636143679 1102 14-15 0.7917516883398577 1102 16-17 0.6159228262745771 1102 18-19 0.5307757323325077 1102 20-21 0.2817313926131817 1102 22-23 0.6104873906534891 1102 24-25 0.64358461988882 1102 26-27 0.4638225786048764 1102 28-29 0.47230884267104045 1102 30-31 0.41316796663807054 1102 32-33 0.48349302076202605 1102 34-35 0.5556317666502721 1102 36-37 0.4983432036364377 1102 38-39 0.5724418443576624 1102 40-41 0.37305973116960445 1102 42-43 0.8605115978400306 1102 44-45 0.783287310997558 1102 46-47 0.3089788508426494 1102 48-49 0.08558842639483544 1102 50-51 0.14118678064528467 1102 52-53 -0.10592399400100305 1102 54-55 0.6024698334839229 1102 56-57 0.21281177939655294 1102 58-59 0.29319120172122126 1102 60-61 0.7842064561492847 1102 62-63 0.6815405963949672 1102 64-65 0.40288705525762936 1102 66-67 0.3715980510518051 1102 68-69 0.6267237292630483 1102 70-71 0.40311365371374563 1102 72-73 0.5841686136007453 1102 74-75 0.48371784951383034 1102 76-77 0.42101420146311597 1102 78-79 0.4265523971970566 1102 80-81 0.2724148645300559 1102 82-83 0.21063052113106195 1102 84-85 0.4907155894283797 1102 86-87 0.3615184244930276 1102 88-89 0.5336808360267646 1102 90-91 0.34268931809811676 1102 92-93 0.2535993016446092 1102 94-95 0.20491904287003848 1102 96-97 0.25658465993734225 1102 98-99 0.3984027310932703 1102 100-101 0.229253729026496 1102 102-103 0.356249386397824 1102 104-105 0.4836302030620452 1102 106-107 0.4772029582043089 1102 108-109 -0.030281740920216293 1102 110-111 0.042505442174267216 1102 112-113 -0.27499742505701974 1102 114-115 0.013125463929931414 1102 116-117 -8.391206821976027E-4 1102 118-119 0.43564555246714676 1102 120-121 0.705269371257657 1102 122-123 0.398643223372801 1102 124-125 0.4911338571146864 1103 1 0.22584054939635934 1103 2 0.681919286613045 1103 3 0.5004309844301389 1103 4 0.4178829425696904 1103 5 0.27648942401349785 1103 6 0.1545415244051398 1103 7 0.2659690601342959 1103 8 0.561353239823724 1103 9 0.39493346776565375 1103 10-11 0.4860709260812186 1103 12-13 0.33162550657408474 1103 14-15 0.4807225698715669 1103 16-17 0.5835135721860212 1103 18-19 0.5307158998634307 1103 20-21 0.36735165587604257 1103 22-23 0.04229832005117373 1103 24-25 0.1005052421464967 1103 26-27 0.06737263843734809 1103 28-29 0.16261598267902855 1103 30-31 0.050104544220836544 1103 32-33 -0.06479178179083078 1103 34-35 -0.9132553494247162 1103 36-37 -0.4139025083699437 1103 38-39 -0.08124782456933843 1103 40-41 -0.26158326843151514 1103 42-43 -0.1853200734802769 1103 44-45 -0.06045022390471644 1103 46-47 0.4030753805594429 1103 48-49 0.434990101703967 1103 50-51 -0.03021329913134707 1103 52-53 -0.2622662357241765 1103 54-55 -0.0292413751319458 1103 56-57 -0.23706855566528162 1103 58-59 0.3581293748365013 1103 60-61 -0.14880112263013245 1103 62-63 0.25401766061515474 1103 64-65 -0.05395379037460657 1103 66-67 0.16790838212480708 1103 68-69 0.059571754791576836 1103 70-71 -0.0776801237094702 1103 72-73 0.1847669382916166 1103 74-75 0.14745937324737213 1103 76-77 0.01720486759793971 1103 78-79 0.03157832460032495 1103 80-81 0.22578542695526593 1103 82-83 0.0552455989132703 1103 84-85 0.24488391810807641 1103 86-87 0.09554315524691503 1103 88-89 0.5263812747982044 1103 90-91 0.626594383913833 1103 92-93 0.17292518915956734 1103 94-95 0.38758757099130037 1103 96-97 0.22946060728477136 1103 98-99 0.14792407134057584 1103 100-101 -0.09063059486660308 1103 102-103 0.02483734012738026 1103 104-105 -0.050534136786378525 1103 106-107 0.08659665585089726 1103 108-109 0.003084832673721394 1103 110-111 0.07102558577219398 1103 112-113 0.15834920438055988 1103 114-115 0.14697069727656142 1103 116-117 0.48446203607887384 1103 118-119 0.25975803750903026 1103 120-121 -0.10901064469766908 1103 122-123 0.23563963342465755 1103 124-125 -0.01225664946688454 1104 1 0.30133494287240126 1104 2 0.8551099084275222 1104 3 0.24711803845664448 1104 4 0.41070659190710046 1104 5 0.589802369986991 1104 6 0.5448575284826092 1104 7 0.5021566238447974 1104 8 0.4255530359501307 1104 9 0.3885318367768633 1104 10-11 0.44008723596909505 1104 12-13 0.5759374331795897 1104 14-15 0.5926695627359919 1104 16-17 0.8396909422166061 1104 18-19 0.6324182444098128 1104 20-21 0.6012966100044821 1104 22-23 0.5964777288177388 1104 24-25 0.2939710933187669 1104 26-27 0.6048853805372403 1104 28-29 0.5917291325261189 1104 30-31 0.2077090294400037 1104 32-33 0.21900026713883847 1104 34-35 0.18930326423481603 1104 36-37 0.2852514161968216 1104 38-39 0.4755819409760207 1104 40-41 0.3696297794787782 1104 42-43 0.27756473793664327 1104 44-45 0.5606303061666438 1104 46-47 0.08733633876535407 1104 48-49 0.2597913249455601 1104 50-51 0.2738437854762026 1104 52-53 0.14151561952557046 1104 54-55 0.14744567889455595 1104 56-57 0.6517489774641874 1104 58-59 0.5412588345714582 1104 60-61 0.29995524841981336 1104 62-63 0.47376281861719605 1104 64-65 0.35882908424314053 1104 66-67 0.24140481433683192 1104 68-69 -0.12612651228283767 1104 70-71 -0.19635494532006703 1104 72-73 -0.1433676182833068 1104 74-75 -0.05203094275670139 1104 76-77 0.09521710001384065 1104 78-79 0.28747027159319316 1104 80-81 0.31729409158319655 1104 82-83 0.029567719198695386 1104 84-85 0.013324285080553722 1104 86-87 0.2045135935751503 1104 88-89 0.06406730945671413 1104 90-91 0.114525066890387 1104 92-93 0.07862345623398426 1104 94-95 -0.07967032911063754 1104 96-97 0.10711606090352177 1104 98-99 -0.16067939451059488 1104 100-101 -0.20451438691553392 1104 102-103 0.02967933808864487 1104 104-105 0.057060154752864634 1104 106-107 0.24377300651348577 1104 108-109 0.15078106101215383 1104 110-111 0.23994505570083646 1104 112-113 -0.20635008206184935 1104 114-115 -0.174507386311614 1104 116-117 -0.09103235739717519 1104 118-119 -0.1362868146826095 1104 120-121 -0.11086589444282424 1104 122-123 0.26598621854188664 1104 124-125 0.10828361556879429 1105 1 0.3116388790956215 1105 2 0.4845070185421214 1105 3 0.1295295979982498 1105 4 0.15902746883784857 1105 5 0.528556729727903 1105 6 0.05018886879152973 1105 7 0.32856419733757036 1105 8 0.3417662895624858 1105 9 0.37814817957706737 1105 10-11 3.363640209101959E-4 1105 12-13 0.22454729865044243 1105 14-15 0.1078464436527824 1105 16-17 0.13873429049762365 1105 18-19 -0.17946018110090023 1105 20-21 0.08111723780717028 1105 22-23 0.013119482679222472 1105 24-25 -0.5525062360285133 1105 26-27 -0.2790707729256354 1105 28-29 -0.57873424565026 1105 30-31 -0.04835474734125711 1105 32-33 -0.26769629489405133 1105 34-35 -0.12057217173927626 1105 36-37 -0.10893891763475949 1105 38-39 -0.3163961357554186 1105 40-41 -0.16990185978379913 1105 42-43 0.11688710963569804 1105 44-45 0.0082336943081458 1105 46-47 0.19634480911911822 1105 48-49 0.04511270013240676 1105 50-51 0.47431214621362017 1105 52-53 0.2718494511150027 1105 54-55 0.31622495144064544 1105 56-57 0.28081723855037666 1105 58-59 0.2543629700971266 1105 60-61 0.13201304612784526 1105 62-63 -0.40792128701310304 1105 64-65 -0.057553576444455246 1105 66-67 0.1401591740777377 1105 68-69 -0.47304230700132166 1105 70-71 0.06260868198435787 1105 72-73 -0.10136462874669405 1105 74-75 -0.02417842656337399 1105 76-77 0.3998209864114486 1105 78-79 0.41170544847410895 1105 80-81 0.44836534220601365 1105 82-83 0.22123687714588058 1105 84-85 -0.0975277328566655 1105 86-87 0.04691518799468497 1105 88-89 0.21337473347265856 1105 90-91 0.2316052861230773 1105 92-93 0.5710101029704049 1105 94-95 0.34327934702289653 1105 96-97 0.28670749089854297 1105 98-99 0.39028223976942655 1105 100-101 0.21070235448954833 1105 102-103 0.10391949753059748 1105 104-105 0.1900447088136552 1105 106-107 0.08648351971726953 1105 108-109 -0.24313024940139982 1105 110-111 0.08866453751448944 1105 112-113 -0.023769115445009703 1105 114-115 0.10682794004613427 1105 116-117 0.3022511503257945 1105 118-119 -0.07851900202690487 1105 120-121 0.2775446685865717 1105 122-123 0.056997678432271215 1105 124-125 0.3741032468592742 1106 1 -0.002575780077620493 1106 2 0.20571641012572783 1106 3 -0.13085381986455857 1106 4 -0.10785721207155063 1106 5 0.285503485950116 1106 6 -0.01137730897493583 1106 7 0.12845453747895164 1106 8 0.23939097869637038 1106 9 0.11181179796075469 1106 10-11 0.030169720200042605 1106 12-13 0.04633044628002381 1106 14-15 -0.1006443625429938 1106 16-17 0.23980739054265854 1106 18-19 0.025858322042026316 1106 20-21 -0.19127980920949028 1106 22-23 -0.11235293590812034 1106 24-25 0.25261252951478497 1106 26-27 0.26315806370075734 1106 28-29 -0.11994481216092368 1106 30-31 -0.1451099904532569 1106 32-33 -0.354944419906289 1106 34-35 -0.2006336595885685 1106 36-37 0.04205879125747458 1106 38-39 0.01585365373681924 1106 40-41 0.09290974066266955 1106 42-43 0.13416832842669635 1106 44-45 0.6997957598299109 1106 46-47 0.012586217465020866 1106 48-49 0.22156716191791048 1106 50-51 0.4336302968784338 1106 52-53 0.025232261932174538 1106 54-55 0.06108954109736686 1106 56-57 0.4418605737766583 1106 58-59 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0.20872791433629345 2216 70-71 0.0107514123745176 2216 72-73 -0.24247635268616108 2216 74-75 0.1565846246823952 2216 76-77 -0.08603067182216506 2216 78-79 -0.38961826078945805 2216 80-81 -0.24768511221953915 2216 82-83 -0.12763964859691157 2216 84-85 0.21289647759391883 2216 86-87 0.2032064040326702 2216 88-89 0.23313445146503753 2216 90-91 -0.16318140963961625 2216 92-93 -0.0029689382995883307 2216 94-95 0.12641404403256118 2216 96-97 -0.1018203621505478 2216 98-99 -0.28675188411249763 2216 100-101 -0.34743773807418066 2216 102-103 -0.19194870706881417 2216 104-105 0.006020344889527962 2216 106-107 -0.1061497504680986 2216 108-109 0.02510072827299581 2216 110-111 0.2775089297353013 2216 112-113 0.03480262144379864 2216 114-115 -0.39901124846253566 2216 116-117 -0.16674239898956955 2216 118-119 -0.46910915574024514 2216 120-121 -0.313028698958572 2216 122-123 -0.8595990458669114 2216 124-125 -0.9677710487211648 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 9.0 4 19.0 5 40.0 6 100.0 7 187.0 8 291.0 9 448.0 10 694.0 11 899.0 12 1024.0 13 1328.0 14 1710.0 15 1233.0 16 561.0 17 487.0 18 424.0 19 419.0 20 437.0 21 416.0 22 489.0 23 578.0 24 804.0 25 1001.0 26 1394.0 27 1757.0 28 2271.0 29 2927.0 30 3622.0 31 4508.0 32 5669.0 33 7232.0 34 10035.0 35 13938.0 36 23360.0 37 34329.0 38 11895.0 39 47.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.15613941254478 18.74464234689018 10.794434634800384 23.30478360576465 2 20.06808697562047 21.746833589574642 34.799033604217 23.38604583058789 3 18.157991068160186 26.127095687824877 32.6363569807453 23.07855626326964 4 12.256387729702029 16.57441979647119 35.92137052492862 35.247821948898164 5 11.647619047619047 40.42197802197802 35.196336996336996 12.734065934065933 6 32.162676623471704 37.08909876272055 16.445567025404493 14.30265758840325 7 25.744197964711912 34.341459843326746 22.98264880298704 16.931693388974303 8 26.589794274837104 33.069770847060546 20.21743905117505 20.1229958269273 9 25.666593454864923 11.415916245698806 20.232813529540962 42.684676769895304 10-11 24.550845596310126 24.642360348488175 29.98462552163409 20.822168533567613 12-13 25.46233252800351 24.73058056958782 29.502891866168827 20.304195036239843 14-15 23.077824145252215 26.513654001024967 26.672889669814776 23.735632183908045 16-17 21.764484092847546 28.233868635583264 27.722482328436666 22.279164943132525 18-19 21.273519291309757 25.875247089830882 31.055714181126 21.79551943773336 20-21 21.98294165019401 25.458305878907677 31.1937184274105 21.36503404348781 22-23 21.651658247309467 25.61424701661908 31.226297679185883 21.50779705688557 24-25 21.73255728823486 26.00409986089758 30.929789882129 21.333552968738562 26-27 21.44776337945677 26.09012372794495 31.13478292700783 21.32732996559045 28-29 21.777948605315174 25.5220001464236 31.071820777509334 21.628230470751888 30-31 21.256680576908998 25.517607438319057 31.540010249652244 21.6857017351197 32-33 21.34197232593894 25.374844424921296 31.514020060033676 21.769163189106084 34-35 22.029877845661634 26.07887136273286 30.939786733240844 20.951464058364664 36-37 22.023208141152352 25.606193718427413 30.062230031481075 22.30836810893916 38-39 21.69229079727652 25.700270883666448 30.621934255802035 21.985504063254997 40-41 22.26297679185885 25.655245625594848 30.621568196793326 21.460209385752986 42-43 22.292669534181385 25.810927061407522 30.03752173515146 21.858881669259635 44-45 22.04682901114349 26.308005447277093 29.69608001054312 21.9490855310363 46-47 22.189234306131116 26.6191738296408 28.587222327978797 22.604369536249287 48-49 22.087749162594037 26.74061464682518 28.510241063093734 22.66139512748705 50-51 22.302637599985356 26.533779949847165 28.498892610693172 22.66468983947431 52-53 22.74258143399984 26.180417767950626 28.391527496101272 22.685473301948267 54-55 22.725292586586228 26.570559401391826 27.8576841272921 22.846463884729854 56-57 22.613639275607962 26.926385690804526 27.751155886325947 22.708819147261565 58-59 23.00922858408408 26.098479717980915 28.012285255131363 22.880006442803644 60-61 22.44926347849006 26.432775939321168 28.180733300143494 22.937227282045274 62-63 22.29828170643317 25.981228356602653 28.743163797963234 22.977326139000944 64-65 22.37169631744637 26.218976499011642 28.556629328647777 22.852697854894206 66-67 21.8829619000205 26.816777462148945 28.192081295575015 23.108179342255543 68-69 23.094550419691263 25.90481629126903 27.967581458175466 23.03305183086424 70-71 22.66890209974522 26.392508858757722 27.902890444255718 23.03569859724134 72-73 22.61289425132515 26.881570855419216 27.66494860456263 22.840586288693004 74-75 22.288764102526557 26.777778591248197 27.882186706103713 23.051270600121534 76-77 22.178498347237525 26.381237210766567 28.20385168698912 23.236412755006793 78-79 22.366571491324404 26.857749469214436 28.01156746467531 22.764111574785854 80-81 21.942309100226957 26.15638040852185 28.473533933670108 23.427776557581083 82-83 22.06640310418039 26.8145545061864 27.538253166410424 23.580789223222784 84-85 21.892525075042098 26.533787246504136 28.26121970861703 23.312467969836735 86-87 21.79925982597491 26.62503340300682 27.847674967695173 23.728031803323095 88-89 21.54016794910278 26.408788409022687 28.089377621916846 23.961666019957683 90-91 21.789329184588635 26.450443854671914 28.389127848448798 23.371099112290654 92-93 21.75195841569661 26.36649827952266 28.129072406471924 23.752470898308808 94-95 21.58181997884171 26.365496615772077 27.797158639573322 24.25552476581289 96-97 21.610451793336214 26.43294848047793 27.813367108625147 24.143232617560713 98-99 21.65900979225771 25.975290564656355 27.523016381440467 24.842683261645465 100-101 21.592435728954275 25.940134490572113 27.74883867354372 24.71859110692989 102-103 22.127615958766963 26.278740313128026 27.524077619436337 24.069566108668674 104-105 21.68687929892122 26.31168574451184 27.46807038608385 24.53336457048309 106-107 21.64909583424848 26.32806208360788 27.54703858261952 24.475803499524122 108-109 21.530284283507456 26.523731779279448 27.108698357847267 24.837285579365833 110-111 21.519589719561168 26.417477185288767 27.099447614933798 24.963485480216267 112-113 21.57464287544755 26.28370809140631 27.316092753538296 24.82555627960784 114-115 20.734069233163087 26.200962893807088 27.724019257876144 25.34094861515368 116-117 20.976942058593092 26.007366239776232 27.40519444383425 25.61049725779643 118-119 21.032805069574863 25.891513733759457 27.42212411086583 25.653557085799843 120-121 20.81605160175569 25.948039492036067 27.69750593954658 25.538402966661668 122-123 20.734997492413175 25.90501920042757 27.57649970165208 25.783483605507172 124-125 20.21137175657471 25.957286358577868 27.574240028114566 26.257101856732852 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 5.5 3 12.5 4 13.0 5 15.0 6 23.5 7 46.0 8 72.5 9 100.5 10 106.0 11 91.5 12 90.5 13 106.0 14 121.0 15 130.0 16 173.5 17 187.5 18 180.5 19 185.5 20 215.0 21 308.5 22 424.0 23 500.0 24 516.5 25 575.0 26 720.5 27 870.0 28 1040.0 29 1323.5 30 1732.0 31 2124.0 32 2341.0 33 2542.0 34 2949.5 35 3256.0 36 3479.0 37 3798.5 38 3985.5 39 3814.5 40 3670.5 41 3779.0 42 4067.0 43 4693.5 44 5002.5 45 4922.0 46 4805.5 47 5026.5 48 5453.5 49 5564.5 50 5562.5 51 5124.0 52 4549.0 53 4340.0 54 4110.0 55 4019.5 56 3977.0 57 3571.5 58 3126.5 59 2701.5 60 2266.5 61 1779.5 62 1391.0 63 1098.5 64 824.5 65 674.0 66 472.0 67 327.0 68 262.0 69 199.0 70 161.0 71 156.5 72 177.0 73 176.5 74 123.5 75 107.5 76 72.5 77 30.5 78 20.5 79 9.5 80 8.5 81 6.5 82 3.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0754081557947141 2 0.0 3 0.0 4 0.0 5 0.06589062156819679 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.660590087122044E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 3.660590087122044E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.001830295043561022 44-45 0.005856944139395271 46-47 0.005856944139395271 48-49 0.005490885130683066 50-51 0.005490885130683066 52-53 0.005124826121970862 54-55 0.004758767113258657 56-57 0.004758767113258657 58-59 0.002562413060985431 60-61 0.0014642360348488177 62-63 7.321180174244088E-4 64-65 0.0 66-67 0.0014642360348488177 68-69 0.0010981770261366134 70-71 0.0014642360348488177 72-73 0.0014642360348488177 74-75 7.321180174244088E-4 76-77 3.660590087122044E-4 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 3.660590087122044E-4 88-89 7.321180174244088E-4 90-91 0.001830295043561022 92-93 0.0 94-95 3.660590087122044E-4 96-97 7.321180174244088E-4 98-99 0.001830295043561022 100-101 3.660590087122044E-4 102-103 3.660590087122044E-4 104-105 3.660590087122044E-4 106-107 0.0 108-109 7.321180174244088E-4 110-111 3.660590087122044E-4 112-113 0.009517534226517315 114-115 0.016472655392049198 116-117 0.015374478365912585 118-119 0.007687239182956293 120-121 0.004026649095834249 122-123 0.0032945310784098397 124-125 0.0043927081045464535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 136590.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.62449666886302 #Duplication Level Percentage of deduplicated Percentage of total 1 49.04135787712168 15.018669009444322 2 14.042553191489363 8.600922468701954 3 9.839827874731053 9.0401932791566 4 6.923260817595027 8.48085511384435 5 4.903179536218025 7.507870268687313 6 3.5405211570643083 6.505600702833298 7 2.433660052593832 5.217072992166337 8 1.7427683480755438 4.269712277619153 9 1.3913459239780062 3.834834175269054 >10 6.074587616543151 29.487517387802914 >50 0.05976571838393497 1.1464968152866242 >100 0.004781257470714798 0.4883227176220807 >500 0.002390628735357399 0.4019327915660004 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 549 0.4019327915660004 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 363 0.2657588403250604 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 304 0.2225638772970203 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.321180174244088E-4 0.0 16-17 0.0 0.0 0.0 7.321180174244088E-4 0.0 18-19 0.0 0.0 0.0 7.321180174244088E-4 0.0 20-21 0.0 0.0 0.0 7.321180174244088E-4 0.0 22-23 0.0 0.0 0.0 0.001830295043561022 0.0 24-25 0.0 0.0 0.0 0.0032945310784098397 0.0 26-27 0.0 0.0 0.0 0.003660590087122044 0.0 28-29 0.0 0.0 0.0 0.005124826121970861 0.0 30-31 0.0 0.0 0.0 0.006955121165531884 0.0 32-33 0.0 0.0 0.0 0.012079947287502744 0.0 34-35 0.0 0.0 0.0 0.02086536349659565 0.0 36-37 0.0 0.0 0.0 0.0420967860019035 0.0 38-39 0.0 0.0 0.0 0.07687239182956293 0.0 40-41 0.0 0.0 0.0 0.1087195255875247 0.0 42-43 0.0 0.0 0.0 0.13800424628450106 0.0 44-45 0.0 0.0 0.0 0.18595797642579984 0.0 46-47 0.0 0.0 0.0 0.230983234497401 0.0 48-49 0.0 0.0 0.0 0.28845449886521707 0.0 50-51 0.0 0.0 0.0 0.3539790614247017 0.0 52-53 0.0 0.0 0.0 0.417307269931913 0.0 54-55 0.0 0.0 0.0 0.48246577348268543 0.0 56-57 0.0 0.0 0.0 0.5864265319569515 0.0 58-59 0.0 0.0 0.0 0.6940478805183397 0.0 60-61 0.0 0.0 0.0 0.800571052053591 0.0 62-63 0.0 0.0 0.0 0.9173438758327842 0.0 64-65 0.0 0.0 0.0 1.0721868365180467 0.0 66-67 0.0 0.0 0.0 1.2167801449593676 0.0 68-69 0.0 0.0 0.0 1.3976132952631963 0.0 70-71 0.0 0.0 0.0 1.6018742221246065 0.0 72-73 0.0 0.0 0.0 1.8006442638553333 0.0 74-75 0.0 0.0 0.0 2.0060033677428804 0.0 76-77 0.0 0.0 0.0 2.25675378871074 0.0 78-79 0.0 0.0 0.0 2.5466725236108063 0.0 80-81 0.0 0.0 0.0 2.888571637748005 0.0 82-83 0.0 0.0 0.0 3.196793323083681 0.0 84-85 0.0 0.0 0.0 3.4965956512189766 0.0 86-87 0.0 0.0 0.0 3.8637528369573175 0.0 88-89 0.0 0.0 0.0 4.282524342924079 0.0 90-91 0.0 0.0 0.0 4.745588988945018 0.0 92-93 0.0 0.0 0.0 5.180101032286405 0.0 94-95 0.0 0.0 0.0 5.634746321106962 0.0 96-97 0.0 0.0 0.0 6.140273812138517 0.0 98-99 0.0 0.0 0.0 6.731825170217439 0.0 100-101 0.0 0.0 0.0 7.377553261585768 0.0 102-103 0.0 0.0 0.0 7.968006442638553 0.0 104-105 0.0 0.0 0.0 8.640456841642873 0.0 106-107 0.0 0.0 0.0 9.331210191082803 0.0 108-109 0.0 0.0 0.0 10.028552602679552 0.0 110-111 0.0 0.0 0.0 10.810454645288821 0.0 112-113 0.0 0.0 0.0 11.58576762574127 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATAG 20 8.4493234E-4 89.29288 2 GGTATCA 445 0.0 78.95447 1 GGGTATA 30 0.004214408 59.550404 1 GGCCGGT 30 0.004220532 59.528584 2 ATAGAGC 30 0.004220532 59.528584 5 CGGTACA 30 0.004220532 59.528584 5 AACGTCA 15 0.0042373305 59.48499 28-29 GTATCAA 650 0.0 54.033638 2 TAGGTAA 35 0.0077668643 51.024498 5 GCCGGTA 35 0.0077668643 51.024498 3 CCGGTAC 35 0.0077668643 51.024498 4 ACGCCTA 25 5.322429E-4 47.60542 50-51 TAGTAGC 50 5.3226284E-4 47.587986 6 ATCAACG 885 0.0 41.031002 4 TTACCTA 30 0.0013083842 39.65666 62-63 AACGCAG 920 0.0 39.441135 7 TCAACGC 925 0.0 39.256687 5 CAACGCA 925 0.0 39.22794 6 TATCAAC 945 0.0 39.05579 3 CATAGAA 65 0.0019283729 36.632977 2 >>END_MODULE