FastQCFastQC Report
Thu 26 May 2016
SRR936189_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936189_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174501
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7330.4200548993988573No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG5780.3312301935232463No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG5650.323780379482066No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT5320.3048693130698391No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA5200.2979925616472112No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4940.2830929335648506No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG4640.26590105500828076No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG4350.2492822390702632No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT4280.2452708007403969No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT4220.24183242502908292No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4130.23667486146211197No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC4130.23667486146211197No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC4120.23610179884355964No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC4090.23438261098790267No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4060.23266342313224567No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3920.22464054647251308No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC3910.22406748385396075No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG3680.21088704362725716No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG3560.20401029220462918No Hit
GTACATGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACAT3320.19025678935937332No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT3110.17822247436977437No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT2960.16962653509148945No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG2870.16446897152451848No Hit
GTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCA2850.16332284628741384No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT2710.15529996962768122No Hit
GGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATAC2680.15358078177202422No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT2650.15186159391636725No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC2620.15014240606071025No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA2570.1472770929679486No Hit
GTACATGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATC2510.14383871725663464No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC2500.1432656546380823No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG2490.14269259201953No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG2480.14211952940097763No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA2450.14040034154532066No Hit
GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT2370.13581584059690202No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA2300.13180440226703571No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT2280.13065827702993105No Hit
GGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTC2270.1300852144113787No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.1295121517928264No Hit
GCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTG2260.1295121517928264No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG2230.1277929639371694No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.1277929639371694No Hit
GTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGA2220.12721990131861707No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT2180.12492765084440778No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT2170.12435458822585543No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG2120.1214892751330938No Hit
GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA2080.11919702465888447No Hit
CCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAACA2080.11919702465888447No Hit
GTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCGTTTGTTG2070.11862396204033215No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG2050.11747783680322749No Hit
GTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGAATA2010.11518558632901818No Hit
TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA2000.11461252371046585No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000.11461252371046585No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA1970.11289333585480885No Hit
CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC1940.11117414799915186No Hit
GACCTATGCTTTGATTCATATATAATCCCAACAAACGACCTAAAACCTGG1890.10830883490639022No Hit
GGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGG1880.10773577228783789No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1860.10658964705073323No Hit
GATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTC1820.10429739657652393No Hit
GGTTAATAGTGTAATTGAATTATAGTGAAATCATATTACTAGACCTGATG1800.10315127133941925No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA1790.10257820872086694No Hit
GCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAAT1790.10257820872086694No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA1780.10200514610231459No Hit
GGTCTTCATAGTCAGTATATTCGTAGCTTCAGTATCATTGGTGCCCTATG1780.10200514610231459No Hit
GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT1750.1002859582466576No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC1750.1002859582466576No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTA208.473425E-489.239461
GATGGCC800.074.366229
TCACTAC250.002054877871.391572
TGGGACG250.002054877871.391576
GAACAAA4350.068.382741
TTACTAA150.00423606259.49298106-107
GTCCAGA150.00423606259.4929810-11
GGGAATT401.7688307E-459.4929737
CTTACTA1650.057.690169
AGGCTTA1750.054.393586
AGAACAA1900.053.230562
ATATAGG1800.052.8826452
ATGGGAT4950.051.6807755
GGGTGTA350.007788872350.9939849
TACCTGG350.007788872350.9939842
ACTACCT505.323197E-447.5943834
GCTTACT2150.047.0409628
GGATGAC1900.046.9681438
GGGATTC653.5102956E-545.7638327
GAACAAG1750.044.1947861
Produced by FastQC (version 0.11.5)