##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936189_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 174501 Sequences flagged as poor quality 0 Sequence length 125 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.427625056589935 31.0 30.0 33.0 15.0 34.0 2 28.513125999277943 31.0 30.0 34.0 16.0 34.0 3 28.867141162514827 31.0 30.0 34.0 18.0 34.0 4 31.912155231202114 35.0 33.0 37.0 17.0 37.0 5 32.69914785588621 35.0 33.0 37.0 26.0 37.0 6 32.18818230268021 36.0 33.0 37.0 18.0 37.0 7 32.29121323086974 36.0 33.0 37.0 18.0 37.0 8 32.056716007358126 37.0 33.0 37.0 17.0 37.0 9 33.15017678981782 39.0 34.0 39.0 13.0 39.0 10-11 33.24532810700225 38.0 33.0 39.0 14.0 39.0 12-13 33.30671170938848 38.0 33.0 39.0 15.0 39.0 14-15 34.31373172646575 39.0 34.0 41.0 14.5 41.0 16-17 34.393315797617205 39.0 33.5 41.0 16.0 41.0 18-19 34.42811789044189 39.0 33.5 41.0 17.5 41.0 20-21 34.55307419441722 39.0 34.0 41.0 18.0 41.0 22-23 34.40145615211374 39.0 33.5 41.0 18.5 41.0 24-25 34.38629864585303 39.0 33.0 41.0 20.5 41.0 26-27 34.433779749113185 39.0 33.0 41.0 22.0 41.0 28-29 34.30311574145707 39.0 33.0 41.0 20.0 41.0 30-31 34.22490988590323 39.0 33.0 41.0 19.5 41.0 32-33 34.02435516128847 39.0 33.0 41.0 17.5 41.0 34-35 33.94381980619022 39.0 33.0 41.0 16.0 41.0 36-37 33.79974899857307 39.0 33.0 41.0 15.5 41.0 38-39 33.51267041449619 38.0 32.5 40.0 9.0 41.0 40-41 33.586887754224904 38.5 33.0 40.5 9.0 41.0 42-43 33.463080440799764 39.0 33.0 41.0 7.5 41.0 44-45 33.351482226462885 39.0 33.0 41.0 2.0 41.0 46-47 33.3448232388353 38.0 33.0 40.0 2.0 41.0 48-49 33.27771760620283 38.5 33.0 40.0 2.0 41.0 50-51 33.18762643194022 38.5 32.5 40.0 2.0 41.0 52-53 33.07271305035501 38.0 32.0 40.0 2.0 41.0 54-55 32.944665073552585 38.0 32.0 40.0 2.0 41.0 56-57 32.80918160927445 38.0 32.0 40.0 2.0 41.0 58-59 32.59174445991714 38.0 31.0 40.0 2.0 41.0 60-61 32.43703761009965 38.0 31.0 40.0 2.0 41.0 62-63 32.21140853061014 38.0 31.0 40.0 2.0 41.0 64-65 31.92008928315597 37.0 31.0 40.0 2.0 41.0 66-67 31.658506254978484 37.0 31.0 40.0 2.0 41.0 68-69 31.409762694769658 36.0 31.0 39.0 2.0 41.0 70-71 31.100145557905115 36.0 30.0 39.0 2.0 40.5 72-73 30.748600294554187 35.5 30.0 39.0 2.0 40.0 74-75 30.366579561148647 35.0 30.0 38.0 2.0 40.0 76-77 30.049993982842505 35.0 30.0 37.0 2.0 39.0 78-79 29.679010435470286 35.0 29.0 37.0 2.0 39.0 80-81 29.37776574346279 35.0 29.0 36.0 2.0 39.0 82-83 29.029263442616376 34.0 29.0 36.0 2.0 37.5 84-85 28.901716895605183 34.0 29.0 36.0 2.0 37.0 86-87 28.756055839221553 34.0 29.0 35.0 2.0 37.0 88-89 28.559291923828518 34.0 29.0 35.0 2.0 36.0 90-91 28.34319001037243 34.0 29.0 35.0 2.0 36.0 92-93 28.19019375247133 34.0 29.0 35.0 2.0 36.0 94-95 28.03846396295723 34.0 29.0 35.0 2.0 36.0 96-97 27.933329894957623 34.0 29.0 35.0 2.0 35.0 98-99 27.841631280049974 34.0 28.5 35.0 2.0 35.0 100-101 27.736316697325517 34.0 28.0 35.0 2.0 35.0 102-103 26.818708775307876 32.5 25.5 34.5 2.0 35.0 104-105 27.435751084521005 34.0 27.0 35.0 2.0 35.0 106-107 27.551859301665893 34.0 27.0 35.0 2.0 35.0 108-109 27.53154423183821 34.0 27.5 35.0 2.0 35.0 110-111 27.43262216262371 34.0 27.0 35.0 2.0 35.0 112-113 27.402310015415384 34.0 27.0 35.0 2.0 35.0 114-115 27.266497040131576 34.0 27.0 35.0 2.0 35.0 116-117 27.162895914636593 34.0 27.0 35.0 2.0 35.0 118-119 27.02771617354628 34.0 26.5 35.0 2.0 35.0 120-121 26.842490873977802 34.0 25.0 35.0 2.0 35.0 122-123 26.5799622924797 34.0 25.0 35.0 2.0 35.0 124-125 24.67893593732987 31.5 20.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3960962884599688 1101 2 0.6703739792121794 1101 3 0.598277745212485 1101 4 0.7402985448230233 1101 5 0.2745423785782961 1101 6 0.6974997200732105 1101 7 0.4381418896287457 1101 8 0.1664417508326217 1101 9 0.7932461236921995 1101 10-11 0.528944587970841 1101 12-13 0.1955534358964499 1101 14-15 0.6035948386315084 1101 16-17 0.8568982801858809 1101 18-19 0.5620087160667282 1101 20-21 0.23450507658198205 1101 22-23 0.35695180710387575 1101 24-25 0.20346352925432853 1101 26-27 0.08410136443345806 1101 28-29 0.028568716402055827 1101 30-31 0.23468358080018703 1101 32-33 0.2814862532402529 1101 34-35 0.21178371450270816 1101 36-37 0.3753011110923268 1101 38-39 0.5693513714822487 1101 40-41 0.5685686956286915 1101 42-43 0.8777658696888153 1101 44-45 0.6221737321335254 1101 46-47 0.6746338934127394 1101 48-49 1.1492753200449215 1101 50-51 0.8172150817276886 1101 52-53 0.9012693202119095 1101 54-55 1.1325306400876798 1101 56-57 1.296208431776499 1101 58-59 1.3437020086811344 1101 60-61 1.0803461087765243 1101 62-63 1.143710939401025 1101 64-65 1.2487017939213914 1101 66-67 0.9865234406476553 1101 68-69 0.6871226650928364 1101 70-71 1.0407764531523007 1101 72-73 1.2551204393864275 1101 74-75 0.9103325371379398 1101 76-77 0.8525370308875786 1101 78-79 1.1081379566972096 1101 80-81 1.0649059194239285 1101 82-83 0.9550379763768362 1101 84-85 0.7941108517147768 1101 86-87 1.0571496266889469 1101 88-89 1.0706204958398367 1101 90-91 1.1698284097825962 1101 92-93 1.3271529123115506 1101 94-95 1.2030171813369606 1101 96-97 1.2598575010466924 1101 98-99 1.4294909690996604 1101 100-101 1.2040699893623383 1101 102-103 1.3728038355814292 1101 104-105 1.172784319099275 1101 106-107 1.1320262120689684 1101 108-109 1.265384337472785 1101 110-111 1.3113356313358082 1101 112-113 1.4894161290994745 1101 114-115 1.3428932246028857 1101 116-117 1.2791700638664913 1101 118-119 1.6410857082179646 1101 120-121 1.5996494329194277 1101 122-123 1.8228588082238666 1101 124-125 1.936415761864982 1102 1 0.36726053212210985 1102 2 0.21067194869433692 1102 3 0.2834273306984869 1102 4 0.37914271158981094 1102 5 -0.4416897492602274 1102 6 -0.04530936319003587 1102 7 -0.0651862372485823 1102 8 -0.21434642650728364 1102 9 0.4643022332680715 1102 10-11 0.13939154219408323 1102 12-13 0.14492866117579695 1102 14-15 0.46222153573592095 1102 16-17 0.19981549753539696 1102 18-19 0.04735654737756079 1102 20-21 -0.11816223087927114 1102 22-23 -0.04655654325056702 1102 24-25 0.11192201778009547 1102 26-27 -0.08058684895113544 1102 28-29 -0.31709390524879666 1102 30-31 -0.09859168052386025 1102 32-33 0.034369828874027064 1102 34-35 -0.23538076945740016 1102 36-37 0.007555106046069682 1102 38-39 -0.05090574734509801 1102 40-41 -0.14249702586596413 1102 42-43 0.21705458769914543 1102 44-45 -0.2867293076357882 1102 46-47 -0.20876752434527646 1102 48-49 -0.3004483372901774 1102 50-51 0.24433871503298832 1102 52-53 0.03751826890829335 1102 54-55 0.019296229418451105 1102 56-57 0.0639183921273343 1102 58-59 -0.2523931493147842 1102 60-61 -0.19123865633220305 1102 62-63 -0.20752058769257076 1102 64-65 0.05324342554460415 1102 66-67 -0.027720221493400032 1102 68-69 -0.07476607694729154 1102 70-71 -0.24746096124154704 1102 72-73 -0.21766581556972042 1102 74-75 0.029256002234660627 1102 76-77 0.02502892083110808 1102 78-79 0.06292589373914481 1102 80-81 -0.18684825575329356 1102 82-83 -0.09064867507095897 1102 84-85 -0.29840386653585327 1102 86-87 -0.1330522236054179 1102 88-89 -0.0022979229995208073 1102 90-91 -0.06220931699861154 1102 92-93 -0.10312463184320109 1102 94-95 -0.2741965637068944 1102 96-97 -0.206560857718177 1102 98-99 -0.29470703642719087 1102 100-101 -0.22498203514805226 1102 102-103 -0.32263651044764785 1102 104-105 -0.22180897658737564 1102 106-107 -0.5100559698365963 1102 108-109 -0.44828861699074807 1102 110-111 -0.6658600913603365 1102 112-113 -0.6580787645686783 1102 114-115 -0.5399014407821916 1102 116-117 -0.006760490020329968 1102 118-119 -0.3664596480237776 1102 120-121 -0.2604911413927198 1102 122-123 -0.24367369207650214 1102 124-125 -0.14122451207470377 1103 1 -0.4016341301358004 1103 2 -0.07456659641666974 1103 3 -0.025618488857489297 1103 4 9.424281698784398E-4 1103 5 -0.30255418005852874 1103 6 0.009060027457103104 1103 7 -0.08677338887824249 1103 8 -0.26751128243548195 1103 9 -0.5834304072609768 1103 10-11 -0.302507371362843 1103 12-13 -0.5115309514836284 1103 14-15 -0.21032215817055544 1103 16-17 -0.2746885175803371 1103 18-19 -0.05642238507402908 1103 20-21 -0.12778669946689547 1103 22-23 -0.34291932076591536 1103 24-25 -0.4937227625694547 1103 26-27 -1.1217181668538316 1103 28-29 -1.03559886509764 1103 30-31 -1.0629752421676884 1103 32-33 -0.9140831706536048 1103 34-35 -1.1847785020979202 1103 36-37 -0.7025889657536482 1103 38-39 -0.9539052749785384 1103 40-41 -0.93433689360333 1103 42-43 -0.8328863664812971 1103 44-45 -0.6117411167996991 1103 46-47 -0.6403570378077248 1103 48-49 -0.5785777657266351 1103 50-51 -0.5625460275272403 1103 52-53 -0.8166976026080022 1103 54-55 -0.6542158159287368 1103 56-57 -0.6619200585103613 1103 58-59 -0.9574828989605102 1103 60-61 -0.6836926005929556 1103 62-63 -0.7601540312447668 1103 64-65 -0.7180957336429259 1103 66-67 -1.0107976896086548 1103 68-69 -0.6755927184210755 1103 70-71 -0.5487717784356896 1103 72-73 -0.504628498611762 1103 74-75 -0.8340293071654372 1103 76-77 -0.6872880371282655 1103 78-79 -0.5614482205735634 1103 80-81 -0.5624269047849388 1103 82-83 -0.5829136727091253 1103 84-85 -0.5519466157092126 1103 86-87 -0.5835954451453347 1103 88-89 -0.6305572522390115 1103 90-91 -0.6406221653689492 1103 92-93 -0.5859777258441454 1103 94-95 -0.3112064834045789 1103 96-97 -0.10636010193168843 1103 98-99 -0.24469522726328208 1103 100-101 -0.34573073614002325 1103 102-103 -0.26005975088222755 1103 104-105 -0.28230732330442976 1103 106-107 -0.2818084497610158 1103 108-109 -0.3451473793903723 1103 110-111 -0.3192965580584932 1103 112-113 -0.3232181127028255 1103 114-115 -0.14518013705049526 1103 116-117 -0.22950493971329067 1103 118-119 -0.3274539796250977 1103 120-121 -0.7670216090356092 1103 122-123 -0.7672920198988962 1103 124-125 -0.8770298970534434 1104 1 0.9617866956137924 1104 2 0.8700401666832427 1104 3 0.7921083048967041 1104 4 1.0989642693424067 1104 5 0.546928529013222 1104 6 1.008006286677336 1104 7 0.908963750694511 1104 8 1.0235846079754758 1104 9 0.879952100642079 1104 10-11 1.081940372673948 1104 12-13 0.8385384996132217 1104 14-15 0.8722408267896924 1104 16-17 0.8662852346947929 1104 18-19 1.2225554863696004 1104 20-21 1.0868052154674857 1104 22-23 0.7858765409066564 1104 24-25 0.7996721986337292 1104 26-27 0.8145903876054135 1104 28-29 0.9420211176593298 1104 30-31 0.7150446831125095 1104 32-33 0.5200221076297638 1104 34-35 0.4689838314589281 1104 36-37 0.6464506932175169 1104 38-39 0.6968275538364779 1104 40-41 0.773357808954394 1104 42-43 0.9431577948368428 1104 44-45 0.812245855132474 1104 46-47 0.7284642094381795 1104 48-49 0.648599166929742 1104 50-51 0.7870683991419867 1104 52-53 0.9129101184380772 1104 54-55 0.705768377959572 1104 56-57 0.8817143892336716 1104 58-59 0.7887691366750857 1104 60-61 0.9384575095708527 1104 62-63 0.704150499840587 1104 64-65 0.7908730373121458 1104 66-67 1.0670953078048875 1104 68-69 0.9685302818663075 1104 70-71 1.196474413728314 1104 72-73 1.279139103648994 1104 74-75 1.2850553752754124 1104 76-77 1.5529768349859197 1104 78-79 1.442766742545512 1104 80-81 1.195452202409239 1104 82-83 1.220479851574808 1104 84-85 1.2612778310768782 1104 86-87 1.224001067930633 1104 88-89 0.9851267213216737 1104 90-91 0.8653889468065472 1104 92-93 1.0716523153475421 1104 94-95 0.9132642513401308 1104 96-97 0.7356865500464735 1104 98-99 0.7675066119933547 1104 100-101 0.9691341934509339 1104 102-103 0.7051787777124687 1104 104-105 0.7269009341915016 1104 106-107 0.6025434996594292 1104 108-109 0.6418006041329569 1104 110-111 0.8152829035757634 1104 112-113 0.4658421421108514 1104 114-115 0.6348634494999423 1104 116-117 0.6631092953666986 1104 118-119 0.671025280840464 1104 120-121 0.7948018414864713 1104 122-123 0.7136448514489366 1104 124-125 0.20741305725252346 1105 1 0.26863984246693917 1105 2 0.07251102915410712 1105 3 0.17607100885940596 1105 4 0.6752346656128019 1105 5 0.2721033541880473 1105 6 0.4259550046260543 1105 7 0.5164229581537185 1105 8 0.32025127464214265 1105 9 0.40533671602668875 1105 10-11 0.40381682955040077 1105 12-13 0.48182521289993474 1105 14-15 0.3358422253910902 1105 16-17 0.45722929063884266 1105 18-19 0.1606324094465208 1105 20-21 0.07333202199428968 1105 22-23 0.1594779395080579 1105 24-25 0.3427840867456169 1105 26-27 0.13924073725575425 1105 28-29 0.05618195682017557 1105 30-31 0.3514083194761426 1105 32-33 0.48523189783954734 1105 34-35 0.5158835983586911 1105 36-37 0.05152062328745188 1105 38-39 0.5344965515054767 1105 40-41 0.4288822844788669 1105 42-43 0.6090086106876598 1105 44-45 0.29821322109983583 1105 46-47 0.31583017680414116 1105 48-49 0.28432177765235167 1105 50-51 0.2391663012398837 1105 52-53 0.22700102752898488 1105 54-55 0.20320427539546415 1105 56-57 -0.03182873430944966 1105 58-59 0.09450340240935162 1105 60-61 0.25381881493215985 1105 62-63 0.09799665368674226 1105 64-65 0.07772618416529298 1105 66-67 0.4046361003456802 1105 68-69 0.26954426788029195 1105 70-71 0.3528263950802959 1105 72-73 0.5157824602923498 1105 74-75 0.6396008298208642 1105 76-77 0.37008639209547667 1105 78-79 0.5826385374173064 1105 80-81 0.4720023189593512 1105 82-83 0.6403283364232948 1105 84-85 0.43435475415380154 1105 86-87 0.11597076490032876 1105 88-89 0.10350667470162733 1105 90-91 0.11284815426575534 1105 92-93 0.16199031068553538 1105 94-95 0.09068849376437171 1105 96-97 -0.07391717955725952 1105 98-99 0.08368376817050915 1105 100-101 0.26070762002436254 1105 102-103 0.575237052770742 1105 104-105 0.24709906938963755 1105 106-107 0.17574862786455725 1105 108-109 0.33366540599775973 1105 110-111 0.22574910404196302 1105 112-113 0.08657640784511855 1105 114-115 0.37440890404539573 1105 116-117 0.3992165214739245 1105 118-119 0.43503460484978973 1105 120-121 0.46939391607854475 1105 122-123 0.3897171125211969 1105 124-125 0.7661892810287476 1106 1 -0.5831409794508708 1106 2 -0.855388514224888 1106 3 -0.5187691737889928 1106 4 -0.4750849690903962 1106 5 -0.2957048649900358 1106 6 -0.5320358629538511 1106 7 -1.0285542107960524 1106 8 -1.3086071728464432 1106 9 -0.9498687634253642 1106 10-11 -0.8470279193080472 1106 12-13 -0.7581062939493819 1106 14-15 -0.7771029800883582 1106 16-17 -0.8003734490871821 1106 18-19 -1.3869018371288178 1106 20-21 -0.9428551926175786 1106 22-23 -0.9405220604919435 1106 24-25 -4.433791255720138 1106 26-27 -1.263841454525057 1106 28-29 -1.1965577692072245 1106 30-31 -0.8198245572361813 1106 32-33 -5.227439335037161 1106 34-35 -1.7755319267554555 1106 36-37 -1.118684856164606 1106 38-39 -0.858014864019637 1106 40-41 -0.9857067566170201 1106 42-43 -0.6205575993580013 1106 44-45 -0.34461782912847383 1106 46-47 -0.7636447090406051 1106 48-49 -0.8474133821649943 1106 50-51 -0.6498747946505219 1106 52-53 -0.850944177950467 1106 54-55 -1.066887048805448 1106 56-57 -1.0215090996062521 1106 58-59 -1.0773459126591405 1106 60-61 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0.36245657582858115 2216 26-27 0.14895689446100135 2216 28-29 0.3619679830210387 2216 30-31 0.1527183631442668 2216 32-33 0.29603975810156413 2216 34-35 0.1328996100035269 2216 36-37 0.2763022826760988 2216 38-39 0.19631605660008944 2216 40-41 -0.02731858888357408 2216 42-43 0.08178881447222608 2216 44-45 0.3816338906777119 2216 46-47 0.34466569354358967 2216 48-49 -0.0656418322457526 2216 50-51 -0.16345378609636185 2216 52-53 -0.35157975849721623 2216 54-55 -0.033986408738449825 2216 56-57 -0.18032946211148726 2216 58-59 0.1702239264268215 2216 60-61 -0.3264140817199461 2216 62-63 -0.5437500712040872 2216 64-65 -0.3515751258783766 2216 66-67 -0.2644541471070134 2216 68-69 -0.32165660548215413 2216 70-71 -0.3032507519357175 2216 72-73 -0.3587590244238079 2216 74-75 -0.35428563306123095 2216 76-77 -0.30294126438196045 2216 78-79 -0.28783453390729363 2216 80-81 -0.5267895296578224 2216 82-83 -0.2495479372302718 2216 84-85 -0.6082216650601708 2216 86-87 -0.3195678086076761 2216 88-89 -0.351522437379888 2216 90-91 -0.27795242797587605 2216 92-93 -0.09749294986759338 2216 94-95 -0.2093042282152524 2216 96-97 -0.44515444302159324 2216 98-99 -0.07267722164026225 2216 100-101 -0.2680478384909222 2216 102-103 -0.4790060331098971 2216 104-105 -0.4202881212945009 2216 106-107 -0.47958616107285224 2216 108-109 -0.5425063552831766 2216 110-111 -0.6800296441385321 2216 112-113 -0.6527030681374129 2216 114-115 -0.6234731920244734 2216 116-117 -0.35714446833684477 2216 118-119 -0.7666102277557449 2216 120-121 -0.9693979325386302 2216 122-123 -0.8786307768528943 2216 124-125 -0.961823819407936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7750.0 3 1296.0 4 1338.0 5 1607.0 6 2192.0 7 1555.0 8 1193.0 9 929.0 10 982.0 11 910.0 12 925.0 13 886.0 14 935.0 15 931.0 16 911.0 17 829.0 18 757.0 19 644.0 20 624.0 21 620.0 22 734.0 23 1043.0 24 1344.0 25 1966.0 26 2468.0 27 2205.0 28 2456.0 29 3074.0 30 3550.0 31 4312.0 32 5819.0 33 7468.0 34 10459.0 35 15029.0 36 25585.0 37 43284.0 38 15875.0 39 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.0237935599223 21.68067804769027 9.66412799926648 20.631400393120956 2 16.801049850717188 26.21016498472788 36.58603675623635 20.402748408318576 3 14.04576478071759 36.97801158732615 29.51501710591916 19.4612065260371 4 8.851525206159277 20.56607125460599 39.32011850934952 31.262285029885216 5 8.41427842820385 48.18081271740563 34.408398805737505 8.996510048653017 6 24.33453103420611 45.68455195099169 18.140870252892533 11.840046761909674 7 24.363757227752274 39.18946023231958 24.145993432702394 12.300789107225746 8 23.797571360622573 39.821548300582805 19.899026366611082 16.48185397218354 9 25.8945558739255 18.554727793696273 19.465329512893984 36.08538681948424 10-11 19.53719330317105 33.77927283875977 29.62799533525692 17.055538522812256 12-13 21.41318051575931 32.809742120343834 28.005444126074497 17.77163323782235 14-15 20.822874760742245 34.24365895311121 24.003426973375053 20.93003931277149 16-17 19.247388237181877 34.981289291056115 27.060040458684576 18.711282013077437 18-19 18.8652051450299 33.992269274520986 27.772445035344145 19.370080545104972 20-21 19.8392527113569 33.658934941761345 27.886072866373446 18.615739480508317 22-23 20.580239620552998 33.12906130454981 27.151451889492368 19.139247185404827 24-25 20.340401146131807 34.0704871060172 27.170487106017195 18.418624641833812 26-27 18.964415574740475 34.3821364408698 27.443474373998928 19.2099736103908 28-29 19.53547411038937 31.84747321640912 29.23745204168469 19.379600631516816 30-31 19.613697381955923 33.73314116578462 28.168973727868973 18.484187724390473 32-33 19.526305082220393 34.22683602625781 27.999346706742962 18.24751218477884 34-35 18.924874140761773 34.06236658347684 27.76610955269469 19.246649723066696 36-37 19.331121311625722 34.0310370714208 26.802998263620264 19.834843353333216 38-39 18.84092022658961 35.11050111604265 27.953501565898094 18.09507709146965 40-41 17.965851100713177 35.13399675072564 29.419973008673328 17.480179139887852 42-43 18.723495702005728 34.489111747851005 27.733237822349572 19.054154727793694 44-45 18.64269340974212 34.746704871060174 27.389971346704872 19.220630372492835 46-47 18.7810888252149 33.79283667621776 28.003438395415476 19.422636103151863 48-49 18.300468192159357 35.295213207947235 27.692391447613478 18.71192715227993 50-51 18.94034687579692 33.921679307509145 28.021696212904835 19.1162776037891 52-53 18.291065068581467 34.488153329073555 27.796481958504415 19.424299643840563 54-55 19.06647564469914 34.91661891117479 27.647277936962748 18.369627507163326 56-57 19.203096839264074 35.03886808347254 26.713103973914116 19.044931103349274 58-59 18.129174414973022 33.631135727404796 28.744330245472078 19.49535961215011 60-61 18.850321774660316 35.51727497263626 27.221620506472743 18.41078274623068 62-63 18.52750716332378 34.74297994269341 28.22234957020057 18.507163323782237 64-65 19.101323201586236 33.46513773560037 28.57175603578203 18.861783027031365 66-67 19.28040526988384 33.94135277161735 27.12907089357654 19.649171064922264 68-69 19.244875387533597 34.01928928774047 27.866315952344113 18.86951937238182 70-71 19.716505922602163 32.21614775846557 28.363734305247533 19.703612013684733 72-73 19.29014733439923 33.44336135609538 27.23222216491596 20.034269144589427 74-75 19.584701520335127 33.37889181150825 27.927060589910656 19.10934607824597 76-77 18.857771588701496 32.957404255563006 29.710717990154784 18.474106165580714 78-79 18.409350089684303 33.00582804683068 28.37634168285569 20.208480180629337 80-81 18.55261574432238 34.40209511693343 27.231362570988132 19.81392656775606 82-83 20.59071294720374 32.24795273379522 27.14253786511252 20.018796453888516 84-85 17.853479349688538 32.33735050228938 28.688947341275984 21.120222806746092 86-87 19.0248193420095 32.84651663887313 28.705566157213998 19.423097861903372 88-89 19.86550220342577 31.688643618088147 29.001266468386998 19.444587710099082 90-91 19.4623526512742 32.803823473790985 28.605280198967343 19.128543675967475 92-93 19.28556283345081 32.89952493108922 28.04425189540461 19.770660340055358 94-95 18.753760723434247 32.30611858957828 28.562300502575916 20.37782018441155 96-97 19.64057512564398 32.90038452501705 28.360009398226943 19.099030951112027 98-99 18.90619537996917 33.303534077168614 28.487802362164118 19.302468180698103 100-101 18.079552552707433 33.24995272233397 28.64281580048252 20.027678924476078 102-103 19.54349701003722 33.08271323004805 27.84777120982461 19.526018550090114 104-105 17.373539406650966 35.45481114721405 27.722477235087478 19.449172211047504 106-107 17.758350955008854 34.378313018263505 28.384078028206144 19.479257998521497 108-109 18.56866149764185 34.95080257419728 26.829645675382952 19.650890252777923 110-111 18.435710970137706 35.13962670701027 26.599274502724914 19.825387820127105 112-113 18.13657228325339 34.49464472982962 28.460008825164323 18.908774161752653 114-115 18.312502507148956 35.35481172027667 27.331361997925512 19.001323774648856 116-117 17.99559887908952 35.142205488793756 27.252852419183842 19.609343212932878 118-119 17.70104469315362 33.23476656294233 28.05485355384783 21.009335190056216 120-121 17.994452753852414 34.044217511647496 27.741961364118257 20.219368370381833 122-123 17.983564564099918 34.166279849399146 27.834224445705182 20.015931140795754 124-125 18.182703824046854 33.76026498415482 27.367178411585037 20.689852780213293 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 426.0 1 583.5 2 832.0 3 798.0 4 534.5 5 365.0 6 309.5 7 278.5 8 254.0 9 214.0 10 199.5 11 182.5 12 161.5 13 164.0 14 153.5 15 135.5 16 141.0 17 150.0 18 162.0 19 191.0 20 226.5 21 291.5 22 347.5 23 439.0 24 488.5 25 565.0 26 796.0 27 981.0 28 1153.5 29 1616.5 30 2442.0 31 3122.5 32 4140.0 33 5771.0 34 7215.0 35 8526.5 36 11470.0 37 13984.0 38 12862.5 39 11696.5 40 10804.0 41 9088.0 42 7209.0 43 6194.0 44 6532.5 45 6925.5 46 5914.5 47 4263.5 48 3664.5 49 3083.0 50 3025.0 51 2578.0 52 1602.5 53 1225.0 54 1063.0 55 943.0 56 831.5 57 765.5 58 743.5 59 673.5 60 589.5 61 534.5 62 416.5 63 316.5 64 237.5 65 165.0 66 161.5 67 142.0 68 96.5 69 74.0 70 68.0 71 60.0 72 46.5 73 53.5 74 58.5 75 40.0 76 22.5 77 22.5 78 25.0 79 17.5 80 12.0 81 10.5 82 7.0 83 3.5 84 2.5 85 3.5 86 4.5 87 5.0 88 4.0 89 4.0 90 3.0 91 1.0 92 1.5 93 1.0 94 0.0 95 1.0 96 1.0 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 5.730626185523292E-4 10-11 2.865313092761646E-4 12-13 5.730626185523292E-4 14-15 0.0017191878556569876 16-17 0.0011461252371046584 18-19 0.001432656546380823 20-21 0.0020057191649331526 22-23 0.08137489183443075 24-25 5.730626185523292E-4 26-27 2.865313092761646E-4 28-29 2.865313092761646E-4 30-31 2.865313092761646E-4 32-33 2.865313092761646E-4 34-35 2.865313092761646E-4 36-37 0.0 38-39 2.865313092761646E-4 40-41 2.865313092761646E-4 42-43 5.730626185523292E-4 44-45 5.730626185523292E-4 46-47 5.730626185523292E-4 48-49 0.0 50-51 2.865313092761646E-4 52-53 2.865313092761646E-4 54-55 5.730626185523292E-4 56-57 2.865313092761646E-4 58-59 2.865313092761646E-4 60-61 0.0 62-63 5.730626185523292E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 2.865313092761646E-4 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 174501.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.819903610867556 #Duplication Level Percentage of deduplicated Percentage of total 1 68.84895621888421 18.465223694993153 2 9.194247986154142 4.931776895261345 3 5.386637037670136 4.334072584111266 4 3.6088972457853465 3.8716110509395363 5 2.482852930492938 3.329493813789033 6 1.6559475224888358 2.664741176268331 7 1.145274673618085 2.1501309448083394 8 0.9251939061131172 1.9850889106652683 9 0.7136599645306724 1.7226262313683016 >10 5.11741202111066 27.845112635457674 >50 0.5790474562509349 10.646357327465171 >100 0.33118950449776713 16.37583738775136 >500 0.010683532403153779 1.67792734712122 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 733 0.4200548993988573 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 578 0.3312301935232463 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 565 0.323780379482066 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 532 0.3048693130698391 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 520 0.2979925616472112 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 494 0.2830929335648506 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 464 0.26590105500828076 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 435 0.2492822390702632 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 428 0.2452708007403969 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 422 0.24183242502908292 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 413 0.23667486146211197 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 413 0.23667486146211197 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 412 0.23610179884355964 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 409 0.23438261098790267 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 406 0.23266342313224567 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 392 0.22464054647251308 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 391 0.22406748385396075 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 368 0.21088704362725716 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 356 0.20401029220462918 No Hit GTACATGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACAT 332 0.19025678935937332 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 311 0.17822247436977437 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 296 0.16962653509148945 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 287 0.16446897152451848 No Hit GTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCA 285 0.16332284628741384 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 271 0.15529996962768122 No Hit GGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATAC 268 0.15358078177202422 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 265 0.15186159391636725 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 262 0.15014240606071025 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 257 0.1472770929679486 No Hit GTACATGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATC 251 0.14383871725663464 No Hit CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC 250 0.1432656546380823 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 249 0.14269259201953 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 248 0.14211952940097763 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 245 0.14040034154532066 No Hit GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT 237 0.13581584059690202 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 230 0.13180440226703571 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 228 0.13065827702993105 No Hit GGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTC 227 0.1300852144113787 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 226 0.1295121517928264 No Hit GCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTG 226 0.1295121517928264 No Hit GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG 223 0.1277929639371694 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 223 0.1277929639371694 No Hit GTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGA 222 0.12721990131861707 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 218 0.12492765084440778 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 217 0.12435458822585543 No Hit CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG 212 0.1214892751330938 No Hit GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA 208 0.11919702465888447 No Hit CCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAACA 208 0.11919702465888447 No Hit GTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCGTTTGTTG 207 0.11862396204033215 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 205 0.11747783680322749 No Hit GTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGAATA 201 0.11518558632901818 No Hit TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA 200 0.11461252371046585 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 200 0.11461252371046585 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 197 0.11289333585480885 No Hit CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC 194 0.11117414799915186 No Hit GACCTATGCTTTGATTCATATATAATCCCAACAAACGACCTAAAACCTGG 189 0.10830883490639022 No Hit GGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGG 188 0.10773577228783789 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 186 0.10658964705073323 No Hit GATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTC 182 0.10429739657652393 No Hit GGTTAATAGTGTAATTGAATTATAGTGAAATCATATTACTAGACCTGATG 180 0.10315127133941925 No Hit GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA 179 0.10257820872086694 No Hit GCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAAT 179 0.10257820872086694 No Hit ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA 178 0.10200514610231459 No Hit GGTCTTCATAGTCAGTATATTCGTAGCTTCAGTATCATTGGTGCCCTATG 178 0.10200514610231459 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 175 0.1002859582466576 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 175 0.1002859582466576 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.730626185523292E-4 0.0 24-25 0.0 0.0 0.0 0.002865313092761646 0.0 26-27 0.0 0.0 0.0 0.003438375711313975 0.0 28-29 0.0 0.0 0.0 0.004584500948418634 0.0 30-31 0.0 0.0 0.0 0.010888189752494256 0.0 32-33 0.0 0.0 0.0 0.026074349144130978 0.0 34-35 0.0 0.0 0.0 0.044412352937805516 0.0 36-37 0.0 0.0 0.0 0.07392507779325047 0.0 38-39 0.0 0.0 0.0 0.11518558632901818 0.0 40-41 0.0 0.0 0.0 0.1587383453389952 0.0 42-43 0.0 0.0 0.0 0.20773519922521932 0.0 44-45 0.0 0.0 0.0 0.25615899049289115 0.0 46-47 0.0 0.0 0.0 0.3065885009254961 0.0 48-49 0.0 0.0 0.0 0.36160251230651974 0.0 50-51 0.0 0.0 0.0 0.4146108045226102 0.0 52-53 0.0 0.0 0.0 0.48452444398599437 0.0 54-55 0.0 0.0 0.0 0.5630340227276635 0.0 56-57 0.0 0.0 0.0 0.6555836356238647 0.0 58-59 0.0 0.0 0.0 0.7704826906436066 0.0 60-61 0.0 0.0 0.0 0.8759262124572351 0.0 62-63 0.0 0.0 0.0 0.978217889868826 0.0 64-65 0.0 0.0 0.0 1.0977014458369867 0.0 66-67 0.0 0.0 0.0 1.2340903490524409 0.0 68-69 0.0 0.0 0.0 1.4017111649789973 0.0 70-71 0.0 0.0 0.0 1.6005638936166555 0.0 72-73 0.0 0.0 0.0 1.7965513091615521 0.0 74-75 0.0 0.0 0.0 1.9911060681600679 0.0 76-77 0.0 0.0 0.0 2.2309327740242177 0.0 78-79 0.0 0.0 0.0 2.5060028309293356 0.0 80-81 0.0 0.0 0.0 2.816316238875422 0.0 82-83 0.0 0.0 0.0 3.114022269213357 0.0 84-85 0.0 0.0 0.0 3.4698941553343534 0.0 86-87 0.0 0.0 0.0 3.8509807966716525 0.0 88-89 0.0 0.0 0.0 4.222038842184285 0.0 90-91 0.0 0.0 0.0 4.654701119191294 0.0 92-93 0.0 0.0 0.0 5.120601028074338 0.0 94-95 0.0 0.0 0.0 5.645526386668271 0.0 96-97 0.0 0.0 0.0 6.177041965375556 0.0 98-99 0.0 0.0 0.0 6.714574701577641 0.0 100-101 0.0 0.0 0.0 7.305402261305092 0.0 102-103 0.0 0.0 0.0 7.921444576248847 0.0 104-105 0.0 0.0 0.0 8.52401991965662 0.0 106-107 0.0 0.0 0.0 9.163271270651745 0.0 108-109 0.0 0.0 0.0 9.787909524873783 0.0 110-111 0.0 0.0 0.0 10.483321012487036 0.0 112-113 0.0 0.0 0.0 11.239190606357557 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCACTA 20 8.473425E-4 89.23946 1 GATGGCC 80 0.0 74.36622 9 TCACTAC 25 0.0020548778 71.39157 2 TGGGACG 25 0.0020548778 71.39157 6 GAACAAA 435 0.0 68.38274 1 TTACTAA 15 0.004236062 59.49298 106-107 GTCCAGA 15 0.004236062 59.49298 10-11 GGGAATT 40 1.7688307E-4 59.492973 7 CTTACTA 165 0.0 57.69016 9 AGGCTTA 175 0.0 54.39358 6 AGAACAA 190 0.0 53.23056 2 ATATAGG 180 0.0 52.882645 2 ATGGGAT 495 0.0 51.680775 5 GGGTGTA 35 0.0077888723 50.993984 9 TACCTGG 35 0.0077888723 50.993984 2 ACTACCT 50 5.323197E-4 47.594383 4 GCTTACT 215 0.0 47.040962 8 GGATGAC 190 0.0 46.968143 8 GGGATTC 65 3.5102956E-5 45.763832 7 GAACAAG 175 0.0 44.194786 1 >>END_MODULE