FastQCFastQC Report
Thu 26 May 2016
SRR936189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174501
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22411.2842333281757699No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18391.0538621555177334No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15670.8979891232714998No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG5360.30716156354404844No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG5280.30257706259562983No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT5080.29111581022458327No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG4860.278508432616432No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT4600.26360880453407143No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG4420.25329367740012954No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4390.25157448954447253No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4330.24813611383315853No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA4270.24469773812184456No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.24355161288473992No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4180.2395401745548736No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC4150.2378209866992166No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.23266342313224567No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT3800.2177637950498851No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC3780.21661766981278044No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG3650.20916785577160013No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC3560.20401029220462918No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC3490.19999885387476288No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.18853760150371632No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT3170.18166085008108837No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG2980.17077266032859412No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA2840.1627497836688615No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.16217672105030917No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG2830.16217672105030917No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT2800.16045753319465217No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG2790.15988447057609986No Hit
GTACATGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACAT2620.15014240606071025No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG2620.15014240606071025No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT2570.1472770929679486No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA2470.1415464667824253No Hit
GGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTC2450.14040034154532066No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA2440.13982727892676833No Hit
GCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAAT2430.139254216308216No Hit
ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT2360.1352427779783497No Hit
GGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTATAG2330.13352359012269271No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG2330.13352359012269271No Hit
GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA2300.13180440226703571No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA2260.1295121517928264No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC2260.1295121517928264No Hit
GTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCA2240.12836602655572177No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT2210.12664683870006477No Hit
GGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT2180.12492765084440778No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG2170.12435458822585543No Hit
CTGTAATCCTTATCATAATTGCTCTCCCCTCTCTACGCATTCTATATATA2140.12263540037019845No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC2130.12206233775164611No Hit
ATGAAGACCTATGCTTTGATTCATATATAATCCCAACAAACGACCTAAAA2020.11575864894757049No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC2010.11518558632901818No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT2010.11518558632901818No Hit
GGTTAATACGGGGTTGTTGATTTCGTCTATTATATATAGAATGCGTAGAG2000.11461252371046585No Hit
GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT2000.11461252371046585No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT1990.1140394610919135No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT1930.11060108538059954No Hit
GCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTG1910.10945496014349487No Hit
GGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATG1900.10888189752494255No Hit
TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA1870.10716270966928557No Hit
GTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGAATA1870.10716270966928557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1860.10658964705073323No Hit
GTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGA1850.10601658443218091No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA1820.10429739657652393No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT1810.10372433395797159No Hit
GGTTAATAGTGTAATTGAATTATAGTGAAATCATATTACTAGACCTGATG1800.10315127133941925No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT1800.10315127133941925No Hit
GGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATAC1790.10257820872086694No Hit
CAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTACCAGCTGTA1770.10143208348376226No Hit
GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT1770.10143208348376226No Hit
GTACATGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATC1760.10085902086520995No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG208.460943E-489.2725752
TGGGATA401.9751387E-674.372496
TCCGGTC250.002054190771.397598
GTCCAAG359.093429E-568.056211
CATGGGT1700.066.516824
GCTTACT1400.063.7478528
GTGGTAC603.1197123E-759.5491831
GGTACTC603.1321179E-759.5150533
TGCTCCG300.004226304659.5150535
TGGTACT603.1321179E-759.5150532
GGTGTAC300.00423110559.4979938
CTCCGGT300.00423110559.4979937
ATGGGTA1550.057.5952035
TGGGTAT1150.056.9111256
ATATAGG1600.055.7953572
AGGCTTA1550.053.7401246
GGTATGC453.1639964E-452.8871048
GGTATCA15050.052.624861
AAGAAAC350.00777745851.01292
GGGTATG952.6120688E-950.1035737
Produced by FastQC (version 0.11.5)