##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936189_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 174501 Sequences flagged as poor quality 0 Sequence length 125 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.403699692265373 31.0 31.0 34.0 30.0 34.0 2 31.372141133861696 31.0 31.0 34.0 30.0 34.0 3 31.638259952665027 31.0 31.0 34.0 30.0 34.0 4 35.51986521567212 37.0 35.0 37.0 33.0 37.0 5 35.23139695474524 37.0 35.0 37.0 33.0 37.0 6 35.423539120119656 37.0 35.0 37.0 33.0 37.0 7 35.2445945868505 37.0 35.0 37.0 32.0 37.0 8 35.340181431625034 37.0 35.0 37.0 33.0 37.0 9 36.96919215362663 39.0 37.0 39.0 33.0 39.0 10-11 36.85820998160469 39.0 37.0 39.0 32.0 39.0 12-13 36.84008687629297 39.0 37.0 39.0 32.0 39.0 14-15 37.83947083397803 40.0 37.0 41.0 32.0 41.0 16-17 37.76744259345219 40.0 37.0 41.0 32.0 41.0 18-19 38.02064458083335 40.0 37.0 41.0 33.0 41.0 20-21 38.10861828872041 40.0 37.0 41.0 33.0 41.0 22-23 37.979762293625825 40.0 37.0 41.0 32.5 41.0 24-25 37.93162789898052 40.0 37.0 41.0 33.0 41.0 26-27 37.71333688632157 40.0 37.0 41.0 32.0 41.0 28-29 37.54480490083151 40.0 36.0 41.0 32.0 41.0 30-31 37.252474197855605 39.0 36.0 41.0 32.0 41.0 32-33 36.85565985295213 39.0 35.0 41.0 31.0 41.0 34-35 36.56012286462541 39.0 35.0 41.0 30.5 41.0 36-37 36.30982917003341 39.0 35.0 41.0 30.0 41.0 38-39 36.229365447762476 39.0 35.0 41.0 30.0 41.0 40-41 36.15598477945685 39.0 35.0 41.0 30.0 41.0 42-43 35.95215213666397 39.0 35.0 41.0 30.0 41.0 44-45 35.63107947805457 39.0 35.0 41.0 28.0 41.0 46-47 35.06491366811652 39.0 35.0 41.0 23.5 41.0 48-49 34.59294216078991 39.0 35.0 41.0 20.5 41.0 50-51 34.4102211448645 39.0 35.0 41.0 18.0 41.0 52-53 34.33677457435774 39.0 35.0 41.0 17.5 41.0 54-55 34.20980682059128 39.0 34.5 41.0 15.0 41.0 56-57 34.09281322170074 39.0 34.0 41.0 14.0 41.0 58-59 33.89722121936264 39.0 34.0 41.0 12.5 41.0 60-61 33.73895565068395 38.0 34.0 40.0 10.5 41.0 62-63 33.51243259350949 38.0 34.0 40.0 8.0 41.0 64-65 33.26443401470479 38.0 33.0 40.0 7.5 41.0 66-67 32.9995272233397 37.0 33.0 40.0 6.0 41.0 68-69 32.72507320874952 37.0 33.0 40.0 2.0 41.0 70-71 32.41560220285271 36.5 33.0 39.0 2.0 41.0 72-73 32.09532323596999 36.0 32.0 39.0 2.0 40.5 74-75 31.83930464581865 36.0 32.0 39.0 2.0 40.0 76-77 31.48571354891949 35.0 32.0 37.5 2.0 40.0 78-79 31.101939243901178 35.0 32.0 37.0 2.0 39.0 80-81 30.776282084343357 35.0 31.5 37.0 2.0 39.0 82-83 30.450060458106257 35.0 31.0 36.0 2.0 38.5 84-85 30.066724545991143 35.0 31.0 36.0 2.0 37.0 86-87 29.848410610827443 35.0 31.0 35.5 2.0 37.0 88-89 29.62632878894677 35.0 31.0 35.0 2.0 36.5 90-91 29.416037730442806 35.0 31.0 35.0 2.0 36.0 92-93 29.234528741955632 34.5 31.0 35.0 2.0 36.0 94-95 29.083684334187197 34.0 31.0 35.0 2.0 36.0 96-97 28.984358255826614 34.0 30.5 35.0 2.0 35.5 98-99 28.893307201677928 34.0 30.0 35.0 2.0 35.0 100-101 28.792281992653336 34.0 30.0 35.0 2.0 35.0 102-103 27.80918733990063 33.5 28.0 34.5 2.0 35.0 104-105 28.4850373350296 34.0 29.5 35.0 2.0 35.0 106-107 28.612082452249556 34.0 30.0 35.0 2.0 35.0 108-109 28.572919352897692 34.0 30.0 35.0 2.0 35.0 110-111 28.515779279201837 34.0 30.0 35.0 2.0 35.0 112-113 28.44498312330588 34.0 30.0 35.0 2.0 35.0 114-115 28.396757038641613 34.0 30.0 35.0 2.0 35.0 116-117 28.297087122709897 34.0 30.0 35.0 2.0 35.0 118-119 28.195915782717577 34.0 30.0 35.0 2.0 35.0 120-121 28.033171729674905 34.0 30.0 35.0 2.0 35.0 122-123 27.8098721497298 34.0 29.0 35.0 2.0 35.0 124-125 25.89587165689595 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34716496873172886 1101 2 0.593433263726908 1101 3 0.7013047128532044 1101 4 0.4319480871830166 1101 5 0.6549307505799149 1101 6 0.6207896006547458 1101 7 0.6684515914666633 1101 8 0.5910765410776406 1101 9 0.42559739589551526 1101 10-11 0.3629736420376162 1101 12-13 0.3683869976001617 1101 14-15 0.5232531748144211 1101 16-17 0.46767490753591545 1101 18-19 0.7378674106520933 1101 20-21 0.6090526004225723 1101 22-23 0.5338823038849512 1101 24-25 0.48409537575408734 1101 26-27 0.4408552294778616 1101 28-29 0.5008101094084338 1101 30-31 0.699947819259755 1101 32-33 0.7337306241645365 1101 34-35 1.0347263856565903 1101 36-37 1.3055270294737653 1101 38-39 0.7908595125262678 1101 40-41 0.9521892732597763 1101 42-43 1.1734614564167032 1101 44-45 1.2953453345136552 1101 46-47 1.1083994587761836 1101 48-49 1.0331581153727143 1101 50-51 1.1094494224703837 1101 52-53 0.9291364929875385 1101 54-55 0.8012026959721226 1101 56-57 0.8885895852547563 1101 58-59 0.786730230321389 1101 60-61 1.1304593312775921 1101 62-63 1.1585691419838753 1101 64-65 0.975158620082901 1101 66-67 0.7977685356435202 1101 68-69 1.1845626850209214 1101 70-71 0.8546945011644098 1101 72-73 0.6294887361848573 1101 74-75 0.8453054292035382 1101 76-77 1.042521564827883 1101 78-79 1.1267553827062713 1101 80-81 1.2205774850565483 1101 82-83 0.8503434732833668 1101 84-85 1.1457739477609827 1101 86-87 0.840420179794517 1101 88-89 0.994263183942266 1101 90-91 0.8380893522207913 1101 92-93 0.959503698024232 1101 94-95 1.1405907735771486 1101 96-97 1.1004818140575594 1101 98-99 0.9986364209549841 1101 100-101 1.0048014286146412 1101 102-103 1.0325919968488257 1101 104-105 0.8850377198967578 1101 106-107 0.8772777787367225 1101 108-109 0.8818106533835142 1101 110-111 1.051354180816606 1101 112-113 1.2217772912316 1101 114-115 1.190411349188448 1101 116-117 1.2072307392073292 1101 118-119 1.4026431203505254 1101 120-121 1.361169920732582 1101 122-123 1.4122703391014113 1101 124-125 1.1998696505273685 1102 1 0.1845313029862048 1102 2 0.5975303441065805 1102 3 0.2532331040583031 1102 4 0.12221601941899252 1102 5 0.3254101450290321 1102 6 0.027253616033817707 1102 7 0.26568816481761814 1102 8 0.2059149406931695 1102 9 0.16990834146802314 1102 10-11 0.14362364804506456 1102 12-13 0.21288417409902394 1102 14-15 0.27500134254239583 1102 16-17 0.5187622558478182 1102 18-19 0.25933803422532264 1102 20-21 0.48776220032645057 1102 22-23 0.26286824645373486 1102 24-25 0.34009681754190524 1102 26-27 0.5650592424539411 1102 28-29 0.5898164773046304 1102 30-31 0.6628458127717138 1102 32-33 0.5124822762130705 1102 34-35 0.6447828556794235 1102 36-37 0.815078873760676 1102 38-39 0.9807669978080185 1102 40-41 0.9793609661589784 1102 42-43 0.6037930676145891 1102 44-45 0.49969472776128043 1102 46-47 0.4247757653963973 1102 48-49 0.04655472717134046 1102 50-51 0.6155409759967512 1102 52-53 0.6874988623255263 1102 54-55 0.5760615209150544 1102 56-57 0.45905095735616186 1102 58-59 0.15317862633244061 1102 60-61 0.4395726317702042 1102 62-63 0.6872847493369889 1102 64-65 0.5292496930133268 1102 66-67 0.6079451546510839 1102 68-69 0.897052171984754 1102 70-71 0.5565387880801893 1102 72-73 0.7075014719772419 1102 74-75 0.9328579943843671 1102 76-77 0.6523677741274163 1102 78-79 0.7049663643234751 1102 80-81 0.3684087958819724 1102 82-83 0.45010317531388466 1102 84-85 0.4411722416454005 1102 86-87 0.5843646709635664 1102 88-89 0.8284936296950001 1102 90-91 0.8315292176539302 1102 92-93 0.3314669324348998 1102 94-95 0.5566967449816893 1102 96-97 0.3648876773280172 1102 98-99 0.5705395808732838 1102 100-101 0.4677655040682005 1102 102-103 0.4014073278592818 1102 104-105 0.3863052916857761 1102 106-107 0.24269289347899914 1102 108-109 0.4080932437956264 1102 110-111 0.6144684425010958 1102 112-113 0.3697828180849001 1102 114-115 0.1684120700823577 1102 116-117 0.5188371311333171 1102 118-119 0.5253993380604847 1102 120-121 0.5288498438372322 1102 122-123 0.5787367574601738 1102 124-125 0.6090129882661657 1103 1 0.0026229420981565 1103 2 0.5636852803370935 1103 3 0.14114052021323786 1103 4 -0.03279106607935489 1103 5 0.10264207700824812 1103 6 0.01048460327992018 1103 7 0.06684546288091298 1103 8 -0.013357614809905272 1103 9 0.2923221345714708 1103 10-11 0.25160664284904044 1103 12-13 0.03964373952179301 1103 14-15 0.15799614651027838 1103 16-17 0.10760259595173949 1103 18-19 0.18379481268540587 1103 20-21 0.07951940391643575 1103 22-23 0.14807136407300447 1103 24-25 -0.03165566238251216 1103 26-27 0.11276826465517331 1103 28-29 -0.06533231816064955 1103 30-31 -0.19594020517821775 1103 32-33 -0.2671941526957653 1103 34-35 -0.16951804181703523 1103 36-37 -0.5913335022431028 1103 38-39 -0.9415995586397798 1103 40-41 -0.8978119200006773 1103 42-43 -0.4321540273310873 1103 44-45 -0.5182315830559077 1103 46-47 -0.5060461524118267 1103 48-49 -0.35105982171859296 1103 50-51 -0.683549718382082 1103 52-53 -0.39606278151009633 1103 54-55 -0.4099328106862785 1103 56-57 -0.32297549517099355 1103 58-59 -0.05362345208040864 1103 60-61 -0.014843995532579868 1103 62-63 -0.6352235170777476 1103 64-65 -0.36567236650485313 1103 66-67 -0.5289939100631287 1103 68-69 -0.4951065431782098 1103 70-71 -0.37056324309600797 1103 72-73 -0.365809817583461 1103 74-75 -0.752923772266552 1103 76-77 -0.6575046869023389 1103 78-79 -0.7688999559410519 1103 80-81 -0.4844962584401813 1103 82-83 -0.644346517647854 1103 84-85 -0.7893544470650404 1103 86-87 -0.6199456738640201 1103 88-89 -0.8743051421519539 1103 90-91 -0.9073583591056362 1103 92-93 -0.9641872952457788 1103 94-95 -0.7225899815180732 1103 96-97 -0.6947367912596079 1103 98-99 -0.972528439910846 1103 100-101 -0.8253379442076607 1103 102-103 -0.7941406173461978 1103 104-105 -0.7709691532835201 1103 106-107 -0.7741469742583611 1103 108-109 -0.8988859720758882 1103 110-111 -0.6147238106873765 1103 112-113 -0.5560674417167064 1103 114-115 -0.690751841112732 1103 116-117 -0.6228728358010258 1103 118-119 -0.4906256973670047 1103 120-121 -0.7568256403384872 1103 122-123 -0.5873000871312293 1103 124-125 -0.5595392082383235 1104 1 0.25178473920516 1104 2 0.49531187292259204 1104 3 0.40519838016151155 1104 4 0.2953773408561773 1104 5 0.6264470391693777 1104 6 0.393663068650163 1104 7 0.31856976597508435 1104 8 0.41589806102111737 1104 9 0.3500144422637774 1104 10-11 0.5542457845292716 1104 12-13 0.723976530018966 1104 14-15 0.4824525120625296 1104 16-17 0.7324589040272258 1104 18-19 0.6146430740131237 1104 20-21 0.6360621762126328 1104 22-23 0.3381877545318588 1104 24-25 0.3369620213036626 1104 26-27 0.3697373229940979 1104 28-29 0.3594210107022633 1104 30-31 0.3098074718023369 1104 32-33 0.765387991187751 1104 34-35 0.693914758259595 1104 36-37 0.8337670821040604 1104 38-39 0.9114036025825598 1104 40-41 0.8876440623731128 1104 42-43 1.0583144083428238 1104 44-45 1.1516841176817039 1104 46-47 1.0632373038579317 1104 48-49 1.6644110088207285 1104 50-51 1.5094884078752244 1104 52-53 1.4787576035842704 1104 54-55 1.2974625336953736 1104 56-57 1.5716504268521874 1104 58-59 1.9181171360985374 1104 60-61 1.5003394511577142 1104 62-63 1.1937351954426063 1104 64-65 1.269230401959561 1104 66-67 1.3456109371444498 1104 68-69 1.6051664714783627 1104 70-71 1.4111806978945154 1104 72-73 1.1159413080032827 1104 74-75 1.2370417416715647 1104 76-77 1.213436016230137 1104 78-79 1.0169871022062864 1104 80-81 1.3285365991132245 1104 82-83 1.3719864644385495 1104 84-85 1.6059540115995823 1104 86-87 1.6012081722898266 1104 88-89 1.2775652477321344 1104 90-91 1.432963989778358 1104 92-93 1.4561594812653809 1104 94-95 1.2266388718203665 1104 96-97 1.23489008870974 1104 98-99 0.9084826722646504 1104 100-101 0.9141725453028293 1104 102-103 1.111896838348791 1104 104-105 1.0343761863083962 1104 106-107 0.9361701059217324 1104 108-109 0.8933235307500524 1104 110-111 0.9793225539069326 1104 112-113 0.9229176143231435 1104 114-115 1.1832782383967988 1104 116-117 1.3554057349433002 1104 118-119 1.2259539558564825 1104 120-121 1.2433060772589855 1104 122-123 1.312881922639825 1104 124-125 1.2433992916179868 1105 1 0.27591061333103184 1105 2 0.6028177004284174 1105 3 0.28438252934565966 1105 4 0.14118153666036903 1105 5 0.2612722139945518 1105 6 0.17897775396485116 1105 7 0.2946536820589998 1105 8 0.20545517057822593 1105 9 0.3523221345714731 1105 10-11 0.15914088942437843 1105 12-13 0.4081140591564889 1105 14-15 0.11557605518607517 1105 16-17 0.4777852443535622 1105 18-19 0.538361022731074 1105 20-21 0.18431392446437656 1105 22-23 0.34419008553419417 1105 24-25 0.6192347485763889 1105 26-27 0.5689098171665918 1105 28-29 0.37809233937359465 1105 30-31 0.40675385874872205 1105 32-33 0.6863903221900785 1105 34-35 0.9751851545299957 1105 36-37 0.8905386438756153 1105 38-39 0.3302497564287137 1105 40-41 0.1787862535152982 1105 42-43 0.17385053887895197 1105 44-45 0.2107866817842705 1105 46-47 0.7631090987297213 1105 48-49 0.683163922573641 1105 50-51 0.5849662633530812 1105 52-53 0.5658321956588566 1105 54-55 0.45922244045528515 1105 56-57 0.41652222172398723 1105 58-59 0.6834085113899135 1105 60-61 0.428078378896636 1105 62-63 0.5410778527852642 1105 64-65 0.5989623366914998 1105 66-67 0.6548417063752296 1105 68-69 0.5168797581916493 1105 70-71 0.36130890302271723 1105 72-73 0.0457198627818336 1105 74-75 0.16038673001655113 1105 76-77 0.04598846378259225 1105 78-79 -0.05118306096387926 1105 80-81 0.06427086484749012 1105 82-83 0.02878133623342194 1105 84-85 0.26094235658792897 1105 86-87 0.1467209928026456 1105 88-89 0.12487294003982896 1105 90-91 0.113943010757378 1105 92-93 0.08428302438892388 1105 94-95 0.38250134268283986 1105 96-97 0.3330485968682453 1105 98-99 0.22760854639052397 1105 100-101 0.229662055792339 1105 102-103 0.4280739945259491 1105 104-105 0.5042363261685345 1105 106-107 0.24407220382382988 1105 108-109 0.40746105988758075 1105 110-111 0.4340661436505222 1105 112-113 0.3589782203837508 1105 114-115 0.41548103559960126 1105 116-117 0.25584862538618935 1105 118-119 0.41241083231335907 1105 120-121 0.2368958208487264 1105 122-123 0.30735744711534707 1105 124-125 0.3720589652776596 1106 1 0.10536266812555439 1106 2 0.5020414447206569 1106 3 0.24045558870638928 1106 4 0.20693496131790567 1106 5 0.5067516660493467 1106 6 0.3324024114990962 1106 7 0.2997221752096806 1106 8 0.3633547139572215 1106 9 0.36423994279064686 1106 10-11 0.5358532181915052 1106 12-13 0.35813689020672257 1106 14-15 0.4045714889760248 1106 16-17 0.4654793082805071 1106 18-19 0.24886330583608895 1106 20-21 0.4151358422726048 1106 22-23 0.5419069805113566 1106 24-25 0.4289607759736498 1106 26-27 0.5271518262990114 1106 28-29 0.38843480512701944 1106 30-31 0.5335118496163034 1106 32-33 0.4639702308658826 1106 34-35 0.28596141023776056 1106 36-37 0.12441992241443245 1106 38-39 0.07209907149720607 1106 40-41 -0.44404479671301544 1106 42-43 0.021613095956581674 1106 44-45 -0.10213569264494993 1106 46-47 -0.3074160154255239 1106 48-49 -0.3099639313076352 1106 50-51 -0.6167688964642721 1106 52-53 -0.5005148363046175 1106 54-55 -0.5058232216451799 1106 56-57 -0.8503727554449725 1106 58-59 -0.5108152329023241 1106 60-61 -0.5645015297791574 1106 62-63 -0.3201550239270645 1106 64-65 -0.37937099664183904 1106 66-67 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-0.13005208678805502 2216 72-73 -0.5483485506097203 2216 74-75 -0.7589655261697636 2216 76-77 -0.6949067327239611 2216 78-79 -0.31794434189546905 2216 80-81 -0.33040162423548125 2216 82-83 -0.32426815430515177 2216 84-85 -0.62292955025341 2216 86-87 -0.47420331578541663 2216 88-89 -0.3336205097418343 2216 90-91 -0.6978037141334497 2216 92-93 -0.5600328416954987 2216 94-95 0.02646786378909738 2216 96-97 -0.38613626484936603 2216 98-99 -0.39424734880597967 2216 100-101 -0.38974842455700554 2216 102-103 -0.68571057607085 2216 104-105 -0.627073717499588 2216 106-107 -0.6078491935560812 2216 108-109 -0.6401299153671509 2216 110-111 -0.45677082869299923 2216 112-113 -0.8742241085827693 2216 114-115 -0.6734490954047665 2216 116-117 -0.8913420587477248 2216 118-119 -0.6734261400301627 2216 120-121 -0.752914950621431 2216 122-123 -1.195393644587707 2216 124-125 -1.3282103214763445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 8.0 4 27.0 5 78.0 6 155.0 7 316.0 8 454.0 9 830.0 10 1364.0 11 1770.0 12 2074.0 13 2621.0 14 3582.0 15 2501.0 16 1024.0 17 903.0 18 775.0 19 770.0 20 915.0 21 925.0 22 953.0 23 1064.0 24 1188.0 25 1484.0 26 1748.0 27 2059.0 28 2614.0 29 3111.0 30 3936.0 31 5052.0 32 6176.0 33 7856.0 34 10362.0 35 14371.0 36 23490.0 37 45980.0 38 21911.0 39 44.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.99408738738222 20.18145010982205 12.160139471133718 20.664323031662015 2 18.640007793651613 23.51104005134641 37.55451258158979 20.294439573412188 3 15.108795938132161 31.741365378994963 34.71441424404445 18.43542443882843 4 9.759829456564718 18.968372674082097 41.003776482656264 30.268021386696926 5 7.757493750430017 43.67990734582483 38.18028576015412 10.382313143591038 6 24.77808150096561 43.6937324141409 18.23370639709801 13.294479687795485 7 22.134543641583715 36.59291350765898 27.031363717113372 14.241179133643932 8 25.79412152365889 33.88863101071054 22.287551360737186 18.029696104893382 9 25.932802677348555 11.385035042779124 22.693279694672235 39.98888258520009 10-11 21.31162572134257 27.883507830900683 31.94451607727176 18.860350370484984 12-13 23.98037833594077 28.04969599028086 31.654832923593563 16.31509275018481 14-15 21.809044074245993 29.08006257843795 30.099540976842537 19.01135237047352 16-17 17.513366685577733 30.03020039999771 32.91757640357362 19.53885651085094 18-19 16.74345705755268 26.933943071959472 37.3356026613028 18.98699720918505 20-21 17.05176474633383 27.338525280657418 38.53387659669572 17.075833376313028 22-23 17.33199236680592 26.627068039724698 38.19290433865709 17.848035254812295 24-25 17.437149357310272 27.826488100354723 37.84333614134017 16.893026400994838 26-27 16.54689657938923 27.47777949696563 38.21496724947135 17.760356674173785 28-29 17.136864545188853 25.05888218405625 39.89232153397402 17.91193173678088 30-31 17.554340662804226 26.93107775886671 38.49175649423213 17.02282508409694 32-33 17.32683480323895 27.080360570999595 38.531011283602965 17.0617933421585 34-35 16.868957771015637 27.006435493206343 38.0545097162767 18.070097019501322 36-37 17.511074435103524 26.647698293992583 36.80150830081203 19.03971897009186 38-39 17.185001805147248 27.241964229431346 37.92241878270038 17.650615182721015 40-41 16.619675531945376 27.39926991822396 38.70378966309649 17.277264886734173 42-43 17.49881803464233 26.774022550466338 36.518574764681446 19.20858465020989 44-45 17.472598323662154 27.38362346872985 35.80944193710151 19.334336270506483 46-47 18.020488573680062 26.309907586503336 35.44953076867971 20.220073071136902 48-49 17.90756516830429 28.566311633011537 33.89840707660303 19.627716122081146 50-51 18.556990535247106 27.582748532146635 33.364471787700694 20.49578914490557 52-53 17.5494648767139 27.955642792670172 33.27220367637578 21.222688654240155 54-55 18.820239207303445 28.204856355269264 32.76779011192426 20.207114325503028 56-57 19.118586758743213 28.50093843574939 31.679011991919424 20.701462813587977 58-59 17.739088575096275 27.293175774802858 33.76610352099762 21.201632129103245 60-61 18.878099238675404 28.748957727430323 32.39970544159405 19.97323759230022 62-63 19.100969059994615 28.210476850870204 33.49245554415784 19.196098544977335 64-65 19.403891723997628 27.444648521333992 34.023501634684536 19.12795811998384 66-67 19.37821458760154 28.0915199358157 32.75920858465021 19.77105689193255 68-69 19.4892792774723 28.435201448723056 33.144124448061156 18.93139482574349 70-71 19.791687559993694 26.674880728950274 34.15521712345449 19.37821458760154 72-73 18.839865441812755 27.687295483589974 32.752425543132546 20.720413531464725 74-75 18.641654106900326 27.890417710130144 33.362368838789905 20.10555934417962 76-77 17.834619385044743 28.066681757358865 34.9471778017828 19.151521055813596 78-79 17.80047105747245 27.945112062395054 33.926166612225714 20.328250267906775 80-81 17.848659459428482 29.309085074254803 32.417385623536894 20.42486984277982 82-83 19.687279729055994 27.105002263597346 32.110131174033384 21.097586833313276 84-85 17.11943518786479 27.72169707250122 33.06236944879814 22.096498290835843 86-87 17.90682548187541 28.623986750754295 33.45319927450065 20.01598849286965 88-89 18.918911174785098 27.26074498567335 33.462464183381094 20.357879656160456 90-91 18.350067049479076 28.43127141858359 33.14250020057536 20.076161331361963 92-93 18.535476975710672 28.543471222145495 32.37068088630119 20.55037091584265 94-95 18.023157526769264 27.6767115280472 32.73429015962705 21.56584078555649 96-97 18.904925229012118 28.165410216075117 32.45969186158126 20.4699726933315 98-99 18.258661990395307 28.253332416417383 32.416703916377266 21.07130167681005 100-101 18.07994269340974 27.695415472779374 32.607449856733524 21.61719197707736 102-103 19.371115676549216 27.20896048412746 31.919862234562274 21.500061604761044 104-105 17.86538070309657 28.86025461391007 31.974590185072167 21.2997744979212 106-107 18.248658313299963 27.865249669771718 32.0440342577815 21.842057759146822 108-109 19.252832394454984 27.963770566020436 30.50504587418839 22.27835116533619 110-111 18.844412607449858 28.415472779369626 30.128653295128938 22.611461318051575 112-113 18.724639508493617 27.590495403590015 31.922697783177828 21.762167304738544 114-115 18.66234116089391 28.136230505132716 31.18433647238191 22.017091861591457 116-117 18.712297282968475 28.06844592445271 30.792928438989303 22.426328353589515 118-119 18.01749137466616 26.61990073702188 31.70397625024357 23.658631638068385 120-121 18.170304337983936 27.698739494594353 31.40374856370008 22.727207603721627 122-123 17.964022097927746 27.729862002567394 31.64456491839355 22.661550981111315 124-125 18.00022923292931 27.625719934668613 31.112639330639848 23.26141150176223 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12.0 1 17.0 2 66.0 3 134.5 4 133.0 5 139.5 6 227.5 7 544.5 8 861.0 9 1048.5 10 942.0 11 608.5 12 498.5 13 478.0 14 491.5 15 504.0 16 468.0 17 468.5 18 463.0 19 452.0 20 511.5 21 524.5 22 537.5 23 636.5 24 657.0 25 663.0 26 820.0 27 1024.5 28 1215.5 29 1641.0 30 2387.5 31 2861.0 32 3813.0 33 5534.5 34 6893.5 35 8213.5 36 10892.0 37 13317.5 38 12696.5 39 11740.5 40 10990.5 41 9056.0 42 6969.0 43 6250.5 44 6648.0 45 6925.5 46 5853.0 47 4273.0 48 3705.0 49 3069.5 50 3160.5 51 2645.0 52 1468.0 53 1119.0 54 942.5 55 853.0 56 791.5 57 687.5 58 646.5 59 611.5 60 518.0 61 482.0 62 409.0 63 258.5 64 177.0 65 148.0 66 126.5 67 109.0 68 101.5 69 78.0 70 67.0 71 59.5 72 30.5 73 29.0 74 37.5 75 20.0 76 8.0 77 8.5 78 11.0 79 8.0 80 2.0 81 0.5 82 5.5 83 6.5 84 1.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07335201517469814 2 0.0 3 0.0 4 0.0 5 0.051002573051157296 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0020057191649331526 44-45 0.007736345350456444 46-47 0.007736345350456444 48-49 0.006017157494799456 50-51 0.006590220113351786 52-53 0.004871032257694798 54-55 0.004584500948418634 56-57 0.004871032257694798 58-59 0.002865313092761646 60-61 0.001432656546380823 62-63 0.0011461252371046584 64-65 0.001432656546380823 66-67 0.0020057191649331526 68-69 0.001432656546380823 70-71 0.0020057191649331526 72-73 0.002292250474209317 74-75 0.0011461252371046584 76-77 2.865313092761646E-4 78-79 0.0 80-81 2.865313092761646E-4 82-83 0.0 84-85 2.865313092761646E-4 86-87 2.865313092761646E-4 88-89 5.730626185523292E-4 90-91 0.0017191878556569876 92-93 2.865313092761646E-4 94-95 2.865313092761646E-4 96-97 8.595939278284938E-4 98-99 0.0017191878556569876 100-101 5.730626185523292E-4 102-103 8.595939278284938E-4 104-105 8.595939278284938E-4 106-107 2.865313092761646E-4 108-109 0.0011461252371046584 110-111 5.730626185523292E-4 112-113 0.009742064515389596 114-115 0.018338003793674534 116-117 0.015759222010189054 118-119 0.008595939278284939 120-121 0.004297969639142469 122-123 0.002865313092761646 124-125 0.003438375711313975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 174501.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.02108297373654 #Duplication Level Percentage of deduplicated Percentage of total 1 58.81358704541737 12.36325293264795 2 11.656943460007634 4.9008315138595195 3 7.077040510332043 4.46301167328554 4 4.917943405484979 4.135219855473608 5 3.334060302055504 3.504277912447493 6 2.4044490485796848 3.032647377378926 7 1.608418297802737 2.3667486146211196 8 1.2267597186631045 2.063025426788385 9 0.9377896516002399 1.774201867038011 >10 6.749904585355216 28.263448347000875 >50 0.8232920778583501 12.263540037019844 >100 0.4334551005942969 16.73285539910946 >500 0.008178398124420696 0.9008544363642614 >1k 0.008178398124420696 3.2360846069650027 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2241 1.2842333281757699 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1839 1.0538621555177334 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1567 0.8979891232714998 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 536 0.30716156354404844 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 528 0.30257706259562983 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 508 0.29111581022458327 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 486 0.278508432616432 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 460 0.26360880453407143 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 442 0.25329367740012954 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 439 0.25157448954447253 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 433 0.24813611383315853 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 427 0.24469773812184456 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 425 0.24355161288473992 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 418 0.2395401745548736 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 415 0.2378209866992166 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 406 0.23266342313224567 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 380 0.2177637950498851 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 378 0.21661766981278044 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 365 0.20916785577160013 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 356 0.20401029220462918 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 349 0.19999885387476288 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 329 0.18853760150371632 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 317 0.18166085008108837 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 298 0.17077266032859412 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 284 0.1627497836688615 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 283 0.16217672105030917 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 283 0.16217672105030917 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 280 0.16045753319465217 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 279 0.15988447057609986 No Hit GTACATGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACAT 262 0.15014240606071025 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 262 0.15014240606071025 No Hit CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT 257 0.1472770929679486 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 247 0.1415464667824253 No Hit GGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTC 245 0.14040034154532066 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 244 0.13982727892676833 No Hit GCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAAT 243 0.139254216308216 No Hit ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT 236 0.1352427779783497 No Hit GGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTATAG 233 0.13352359012269271 No Hit GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG 233 0.13352359012269271 No Hit GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA 230 0.13180440226703571 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 226 0.1295121517928264 No Hit CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC 226 0.1295121517928264 No Hit GTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCA 224 0.12836602655572177 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 221 0.12664683870006477 No Hit GGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT 218 0.12492765084440778 No Hit CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG 217 0.12435458822585543 No Hit CTGTAATCCTTATCATAATTGCTCTCCCCTCTCTACGCATTCTATATATA 214 0.12263540037019845 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 213 0.12206233775164611 No Hit ATGAAGACCTATGCTTTGATTCATATATAATCCCAACAAACGACCTAAAA 202 0.11575864894757049 No Hit CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 201 0.11518558632901818 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 201 0.11518558632901818 No Hit GGTTAATACGGGGTTGTTGATTTCGTCTATTATATATAGAATGCGTAGAG 200 0.11461252371046585 No Hit GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT 200 0.11461252371046585 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 199 0.1140394610919135 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 193 0.11060108538059954 No Hit GCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTG 191 0.10945496014349487 No Hit GGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATG 190 0.10888189752494255 No Hit TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA 187 0.10716270966928557 No Hit GTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGAATA 187 0.10716270966928557 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 186 0.10658964705073323 No Hit GTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGA 185 0.10601658443218091 No Hit GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA 182 0.10429739657652393 No Hit AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT 181 0.10372433395797159 No Hit GGTTAATAGTGTAATTGAATTATAGTGAAATCATATTACTAGACCTGATG 180 0.10315127133941925 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 180 0.10315127133941925 No Hit GGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATAC 179 0.10257820872086694 No Hit CAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTACCAGCTGTA 177 0.10143208348376226 No Hit GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT 177 0.10143208348376226 No Hit GTACATGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATC 176 0.10085902086520995 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.730626185523292E-4 0.0 24-25 0.0 0.0 0.0 0.002865313092761646 0.0 26-27 0.0 0.0 0.0 0.003438375711313975 0.0 28-29 0.0 0.0 0.0 0.004584500948418634 0.0 30-31 0.0 0.0 0.0 0.010888189752494256 0.0 32-33 0.0 0.0 0.0 0.02636088045340714 0.0 34-35 0.0 0.0 0.0 0.045845009484186336 0.0 36-37 0.0 0.0 0.0 0.07564426564890746 0.0 38-39 0.0 0.0 0.0 0.11690477418467515 0.0 40-41 0.0 0.0 0.0 0.15988447057609984 0.0 42-43 0.0 0.0 0.0 0.208881324462324 0.0 44-45 0.0 0.0 0.0 0.25787817834854815 0.0 46-47 0.0 0.0 0.0 0.30859422009042925 0.0 48-49 0.0 0.0 0.0 0.3653274193271099 0.0 50-51 0.0 0.0 0.0 0.4183357115432003 0.0 52-53 0.0 0.0 0.0 0.487389757078756 0.0 54-55 0.0 0.0 0.0 0.5679050549853583 0.0 56-57 0.0 0.0 0.0 0.6641795749021495 0.0 58-59 0.0 0.0 0.0 0.7830900682517579 0.0 60-61 0.0 0.0 0.0 0.8891066526839387 0.0 62-63 0.0 0.0 0.0 0.9896791422398725 0.0 64-65 0.0 0.0 0.0 1.1111684173729663 0.0 66-67 0.0 0.0 0.0 1.2492765084440776 0.0 68-69 0.0 0.0 0.0 1.4180434496077385 0.0 70-71 0.0 0.0 0.0 1.6229133357401964 0.0 72-73 0.0 0.0 0.0 1.8249179087798924 0.0 74-75 0.0 0.0 0.0 2.0266359505103124 0.0 76-77 0.0 0.0 0.0 2.275345126962023 0.0 78-79 0.0 0.0 0.0 2.5615899049289115 0.0 80-81 0.0 0.0 0.0 2.8853702844109774 0.0 82-83 0.0 0.0 0.0 3.18852040962516 0.0 84-85 0.0 0.0 0.0 3.553561297642993 0.0 86-87 0.0 0.0 0.0 3.9420977530214727 0.0 88-89 0.0 0.0 0.0 4.317453768173248 0.0 90-91 0.0 0.0 0.0 4.757852390530713 0.0 92-93 0.0 0.0 0.0 5.231488644764213 0.0 94-95 0.0 0.0 0.0 5.767588724419918 0.0 96-97 0.0 0.0 0.0 6.30827330502404 0.0 98-99 0.0 0.0 0.0 6.858986481452828 0.0 100-101 0.0 0.0 0.0 7.468152044973954 0.0 102-103 0.0 0.0 0.0 8.096515206216583 0.0 104-105 0.0 0.0 0.0 8.702242394026396 0.0 106-107 0.0 0.0 0.0 9.35123580953691 0.0 108-109 0.0 0.0 0.0 9.990200629222755 0.0 110-111 0.0 0.0 0.0 10.703090526701853 0.0 112-113 0.0 0.0 0.0 11.470134841634145 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 20 8.460943E-4 89.272575 2 TGGGATA 40 1.9751387E-6 74.37249 6 TCCGGTC 25 0.0020541907 71.39759 8 GTCCAAG 35 9.093429E-5 68.05621 1 CATGGGT 170 0.0 66.51682 4 GCTTACT 140 0.0 63.747852 8 GTGGTAC 60 3.1197123E-7 59.549183 1 GGTACTC 60 3.1321179E-7 59.515053 3 TGCTCCG 30 0.0042263046 59.515053 5 TGGTACT 60 3.1321179E-7 59.515053 2 GGTGTAC 30 0.004231105 59.497993 8 CTCCGGT 30 0.004231105 59.497993 7 ATGGGTA 155 0.0 57.595203 5 TGGGTAT 115 0.0 56.911125 6 ATATAGG 160 0.0 55.795357 2 AGGCTTA 155 0.0 53.740124 6 GGTATGC 45 3.1639964E-4 52.887104 8 GGTATCA 1505 0.0 52.62486 1 AAGAAAC 35 0.007777458 51.0129 2 GGGTATG 95 2.6120688E-9 50.103573 7 >>END_MODULE