FastQCFastQC Report
Thu 26 May 2016
SRR936188_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936188_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343384
Sequences flagged as poor quality0
Sequence length125
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34080.9924748969084175No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21710.6322367961232905No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG16430.4784730797008597No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG14260.41527852200451976No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT13430.39110733173356943No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10900.3174288842811546No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT10870.31655522680148174No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9730.2833562425739114No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC9180.2673391887799082No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG8900.2591850523029611No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC8620.25103091582601406No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC8510.24782750506721338No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA8100.2358875195116837No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG7890.22977191715397338No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA7530.21928802739789857No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT7460.2172494932786618No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT7460.2172494932786618No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC7360.21433730167975212No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT7000.20385341192367726No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA6920.20152365864454955No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT6780.19744659040607598No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG6760.19686415208629407No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA6670.19424317964727536No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6650.19366074132749342No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT6590.1919134263681476No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG6360.18521538569065535No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG6230.18142953661207278No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC6210.18084709829229084No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT6150.17909978333294505No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6030.17560515341425342No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC5720.16657735945763344No Hit
GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT5570.16220907205926893No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC5550.161626633739487No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5510.1604617570999231No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG5480.15958809962025022No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT5080.14793933322461153No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4980.14502714162570185No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT4800.13978519674766443No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG4740.1380378817883186No Hit
GGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTC4740.1380378817883186No Hit
GAGTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCA4730.13774666262842766No Hit
GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA4570.13308715607017219No Hit
GTACATGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACAT4530.1319222794306083No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA4530.1319222794306083No Hit
TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA4510.13133984111082636No Hit
GGCTAATATTTATTAATACTAGAGTAGCTCCTCCGATTAGGTGTATTAAT4490.13075740279104442No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT4250.1237681429536612No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA4210.12260326631409733No Hit
GGTCTAGTAATATGATTTCACTATAATTCAATTACACTATTAACCCTTGG4160.1211471705146425No Hit
CCTTCTAACATCAGGTCTAGTAATATGATTTCACTATAATTCAATTACAC4070.11852619807562378No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG4060.11823497891573283No Hit
GGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATG3980.11590522563660509No Hit
GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG3960.11532278731682315No Hit
TTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAATGGTAGCTGTTG3960.11532278731682315No Hit
CAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGT3870.11270181487780444No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA3840.11182815739813154No Hit
CCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGA3810.11095449991845863No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT3810.11095449991845863No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC3770.10978962327889477No Hit
GTCCATGACCATTAACTGGAGCCTTTTCAGCCCTCCTTCTAACATCAGGT3770.10978962327889477No Hit
GGTTAATAGTGTAATTGAATTATAGTGAAATCATATTACTAGACCTGATG3710.10804230831954896No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG3690.10745986999976702No Hit
GTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGA3670.10687743167998509No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA3640.10600377420031219No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3630.10571255504042121No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA3570.10396524008107542No Hit
ATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATT3530.10280036344151156No Hit
GCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGT3490.10163548680194767No Hit
GTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATT3490.10163548680194767No Hit
GGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAA3470.10105304848216573No Hit
GTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAA3450.1004706101623838No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC3440.10017939100249285No Hit
GTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGAATA3440.10017939100249285No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACAT358.949064E-784.993457
TGGGTAT1800.072.716616
ATAGGCC250.002056244371.39454
ACCGACT250.002056244371.39458
CAGGGTA250.002056244371.39454
GTATGGT701.7462298E-1067.994761
TACCTGG551.7248203E-764.904092
TATGGTG753.2196112E-1063.4617732
GAACAAA5950.061.995221
ATGGGTA2550.060.6619875
CATGGGT3400.059.495414
GGGACCG300.00423531959.495417
GGTATGG1500.059.495418
ACATCGG300.00423531959.495418
GGATGAC4750.057.616618
GATGACC4250.055.9956869
GGGATGA4950.055.2886667
GATGGCC1103.6379788E-1254.086749
CGCAGAA453.169E-452.8848082
GGTTACA453.169E-452.8848081
Produced by FastQC (version 0.11.5)