##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936188_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 343384 Sequences flagged as poor quality 0 Sequence length 125 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.13899890501596 31.0 30.0 33.0 12.0 34.0 2 28.454208699298743 31.0 30.0 34.0 16.0 34.0 3 28.621945111012742 31.0 28.0 34.0 16.0 34.0 4 31.727812012207906 35.0 33.0 37.0 16.0 37.0 5 32.565428208652705 35.0 33.0 37.0 25.0 37.0 6 32.2350313351816 36.0 33.0 37.0 18.0 37.0 7 32.42665936677306 36.0 33.0 37.0 19.0 37.0 8 32.1597191482422 37.0 33.0 37.0 17.0 37.0 9 33.31957516948955 39.0 34.0 39.0 13.0 39.0 10-11 33.3953110803066 38.5 33.5 39.0 16.0 39.0 12-13 33.505434149523566 39.0 34.0 39.0 16.0 39.0 14-15 34.55272086061086 39.0 34.0 41.0 17.0 41.0 16-17 34.62186502574377 39.0 34.0 41.0 17.5 41.0 18-19 34.614805873308015 39.0 34.0 41.0 17.5 41.0 20-21 34.62336771660881 39.0 34.0 41.0 18.5 41.0 22-23 34.38167037485731 39.0 33.5 41.0 18.0 41.0 24-25 34.277839095589776 39.0 33.5 41.0 18.0 41.0 26-27 34.27652569717867 39.0 34.0 41.0 18.0 41.0 28-29 34.12218245462806 39.0 33.0 41.0 16.0 41.0 30-31 34.04094541388067 39.0 33.0 41.0 16.0 41.0 32-33 33.81519377722899 39.0 33.0 41.0 10.0 41.0 34-35 33.761597803042655 39.0 33.0 41.0 9.0 41.0 36-37 33.67039524264381 39.0 33.0 41.0 8.0 41.0 38-39 33.4292060783263 39.0 33.0 41.0 4.5 41.0 40-41 33.498839491647836 39.0 33.0 41.0 2.0 41.0 42-43 33.40533193159844 39.0 33.0 41.0 2.0 41.0 44-45 33.35291102672227 39.0 33.0 41.0 2.0 41.0 46-47 33.3685931784824 39.0 33.0 41.0 2.0 41.0 48-49 33.31018626377467 39.0 33.0 41.0 2.0 41.0 50-51 33.21814208000373 39.0 33.0 41.0 2.0 41.0 52-53 33.14964442140577 39.0 33.0 40.5 2.0 41.0 54-55 33.05834867087576 39.0 33.0 40.0 2.0 41.0 56-57 32.951175651748486 38.5 32.0 40.0 2.0 41.0 58-59 32.766895079561074 38.0 32.0 40.0 2.0 41.0 60-61 32.64506063182909 38.0 32.0 40.0 2.0 41.0 62-63 32.42503581995667 38.0 31.0 40.0 2.0 41.0 64-65 32.19624676746733 38.0 31.0 40.0 2.0 41.0 66-67 31.943315646623024 37.0 31.0 40.0 2.0 41.0 68-69 31.710829566898866 37.0 31.0 39.5 2.0 41.0 70-71 31.41872510076183 36.0 31.0 39.0 2.0 41.0 72-73 31.08050316846446 36.0 30.5 39.0 2.0 40.5 74-75 30.738370162849755 35.5 30.0 38.5 2.0 40.0 76-77 30.40901002958787 35.0 30.0 37.5 2.0 40.0 78-79 30.05755801085665 35.0 30.0 37.0 2.0 39.0 80-81 29.752149197399994 35.0 30.0 37.0 2.0 39.0 82-83 29.397027817254152 35.0 29.5 36.0 2.0 38.5 84-85 29.239117139994875 35.0 30.0 36.0 2.0 37.0 86-87 29.084657409780306 35.0 30.0 36.0 2.0 37.0 88-89 28.877714162570186 35.0 29.5 35.0 2.0 36.5 90-91 28.66431604268108 34.0 29.0 35.0 2.0 36.0 92-93 28.50087948186287 34.0 29.0 35.0 2.0 36.0 94-95 28.347717133005617 34.0 29.0 35.0 2.0 36.0 96-97 28.235354588449084 34.0 29.0 35.0 2.0 35.5 98-99 28.149788574889918 34.0 29.0 35.0 2.0 35.0 100-101 28.063238240570325 34.0 29.0 35.0 2.0 35.0 102-103 27.183230727115998 33.5 26.5 34.5 2.0 35.0 104-105 27.79145213521888 34.0 28.5 35.0 2.0 35.0 106-107 27.89670747617827 34.0 29.0 35.0 2.0 35.0 108-109 27.869857069636325 34.0 29.0 35.0 2.0 35.0 110-111 27.774281853551706 34.0 28.0 35.0 2.0 35.0 112-113 27.759665563916784 34.0 28.0 35.0 2.0 35.0 114-115 27.66971087761806 34.0 28.0 35.0 2.0 35.0 116-117 27.559332991636186 34.0 28.0 35.0 2.0 35.0 118-119 27.414439810824035 34.0 27.5 35.0 2.0 35.0 120-121 27.2574814202176 34.0 27.0 35.0 2.0 35.0 122-123 27.03031009016145 34.0 27.0 35.0 2.0 35.0 124-125 25.179108519907743 31.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8108025191953772 1101 2 0.739343242665079 1101 3 0.8362669089171888 1101 4 0.8526660368088521 1101 5 0.5711779175837677 1101 6 0.6518609610259389 1101 7 0.7130669312174192 1101 8 0.646030007368239 1101 9 0.9709952930973813 1101 10-11 1.006992098316232 1101 12-13 0.6322259740662588 1101 14-15 0.8190661322254016 1101 16-17 0.8595871813879086 1101 18-19 0.8738567420334249 1101 20-21 0.6667508906155248 1101 22-23 0.8925490857849994 1101 24-25 0.8934977402483284 1101 26-27 1.043328231244523 1101 28-29 1.049668776214567 1101 30-31 1.0184011028552433 1101 32-33 1.1925358533297583 1101 34-35 1.1481046737944496 1101 36-37 1.2040585951346472 1101 38-39 1.3060881310382513 1101 40-41 1.3005030895880907 1101 42-43 1.4715397553029703 1101 44-45 1.381079834982451 1101 46-47 1.2392508921459182 1101 48-49 1.263693149124279 1101 50-51 1.28126621595176 1101 52-53 1.285385323048871 1101 54-55 1.2362915167646662 1101 56-57 1.437482781420755 1101 58-59 1.4137601611665858 1101 60-61 1.4316051203021019 1101 62-63 1.5967050505184943 1101 64-65 1.4591701806118138 1101 66-67 1.4138906919638181 1101 68-69 1.3552541935961138 1101 70-71 1.2700253447554068 1101 72-73 1.28880798099976 1101 74-75 1.1603069266541546 1101 76-77 1.154269996844146 1101 78-79 1.1131555030828473 1101 80-81 1.1456499514113574 1101 82-83 1.0601839062242995 1101 84-85 0.9971625323339524 1101 86-87 1.1555887023969653 1101 88-89 1.1028375611885934 1101 90-91 1.1414312664871495 1101 92-93 1.1920653499726725 1101 94-95 1.2281190139276639 1101 96-97 1.2299845618515306 1101 98-99 1.200737413244017 1101 100-101 1.2952518190625355 1101 102-103 1.3427551245722142 1101 104-105 1.3875736281033006 1101 106-107 1.3479193260395945 1101 108-109 1.292264494087803 1101 110-111 1.3374225361471694 1101 112-113 1.2514302748265642 1101 114-115 1.1972039786019586 1101 116-117 1.2172214717097596 1101 118-119 1.0249559806058741 1101 120-121 1.117473474474135 1101 122-123 1.4470206402670769 1101 124-125 1.2255501263253699 1102 1 0.3776098328381323 1102 2 0.1903436509950076 1102 3 0.2055980904166006 1102 4 0.4971370551059202 1102 5 0.2752618650323555 1102 6 0.3083550272790916 1102 7 0.27413170012211907 1102 8 0.4989502145146645 1102 9 0.2981291410560516 1102 10-11 0.3923113410262502 1102 12-13 0.6191253887933641 1102 14-15 0.5511449852719963 1102 16-17 0.16877395668330308 1102 18-19 0.36042884467330794 1102 20-21 0.3747220741889876 1102 22-23 0.7091408919643882 1102 24-25 0.8792450812427077 1102 26-27 0.8439206337152427 1102 28-29 0.5787266359175831 1102 30-31 0.5863588698738553 1102 32-33 0.5675755520730092 1102 34-35 0.4399098809732749 1102 36-37 0.6272228928395762 1102 38-39 0.6460179371787618 1102 40-41 0.6208654661979196 1102 42-43 0.7600572583978504 1102 44-45 0.6818715413478671 1102 46-47 0.5415398082568004 1102 48-49 0.6938541477826234 1102 50-51 0.29696585363996775 1102 52-53 0.5264117867172331 1102 54-55 0.6312535031925535 1102 56-57 0.5766114831260225 1102 58-59 0.6116527250248467 1102 60-61 0.6140184150061216 1102 62-63 0.651879822210212 1102 64-65 0.6423207376905111 1102 66-67 0.5735720001751439 1102 68-69 0.7013121894091121 1102 70-71 0.6813156673360474 1102 72-73 0.5635281453687604 1102 74-75 0.6189926876061449 1102 76-77 0.5908330254855763 1102 78-79 0.5540400234896232 1102 80-81 0.701523096912922 1102 82-83 0.6104489966078042 1102 84-85 0.4440715395737058 1102 86-87 0.45797808063362666 1102 88-89 0.6577044650755042 1102 90-91 0.6875984743530132 1102 92-93 0.7651920553982734 1102 94-95 0.5831861939233889 1102 96-97 0.7069560500520922 1102 98-99 0.7823450665135745 1102 100-101 0.6943813633533722 1102 102-103 0.7037220239221327 1102 104-105 0.7379288103286363 1102 106-107 0.8734577706266329 1102 108-109 0.7548083247410631 1102 110-111 0.5852350543761844 1102 112-113 0.6826380240212444 1102 114-115 0.7846416758804047 1102 116-117 0.7359317324575869 1102 118-119 0.592278567732464 1102 120-121 0.5675762050995239 1102 122-123 0.7425721125233267 1102 124-125 0.7509411184374741 1103 1 0.055007155486343606 1103 2 0.2630146281373449 1103 3 0.179605141556884 1103 4 0.4802865210666063 1103 5 0.24050052401474886 1103 6 0.35980428378708496 1103 7 0.4416775910766262 1103 8 0.6432709243121053 1103 9 0.4896319966377689 1103 10-11 0.507230617178557 1103 12-13 0.5244960289900646 1103 14-15 0.7921521795165987 1103 16-17 0.5471629272428373 1103 18-19 0.579485787184403 1103 20-21 0.29247611688612096 1103 22-23 0.6244377683873026 1103 24-25 0.5711008190755891 1103 26-27 0.5298229064720772 1103 28-29 0.7605097808302261 1103 30-31 0.7666605598058069 1103 32-33 0.8849077622542367 1103 34-35 0.7321825727655948 1103 36-37 0.6089218013438753 1103 38-39 0.8553551690207684 1103 40-41 1.0072731574048035 1103 42-43 0.9879427619299292 1103 44-45 0.8457113555724405 1103 46-47 0.6017802235195262 1103 48-49 0.7132464097415934 1103 50-51 0.817008589794618 1103 52-53 0.762390815429157 1103 54-55 0.7207620048902896 1103 56-57 0.6687332649049367 1103 58-59 0.6685692171476134 1103 60-61 0.5088529994181599 1103 62-63 0.48664148846491173 1103 64-65 0.4512797187877311 1103 66-67 0.40673512609393825 1103 68-69 0.3470671948080444 1103 70-71 0.48707106301950276 1103 72-73 0.44090888092498304 1103 74-75 0.5418919811911707 1103 76-77 0.39252133126048605 1103 78-79 0.43981675472669224 1103 80-81 0.5594253826792439 1103 82-83 0.38611670836967704 1103 84-85 0.3384507920487607 1103 86-87 0.266719152875293 1103 88-89 0.3883349697133873 1103 90-91 0.25278411968846015 1103 92-93 0.15820856443335884 1103 94-95 0.172257251724524 1103 96-97 0.39852070068240053 1103 98-99 0.022592832134709084 1103 100-101 0.13066091114370693 1103 102-103 0.21657864639991686 1103 104-105 0.20969335167927738 1103 106-107 0.2620364194616336 1103 108-109 0.49660747131891725 1103 110-111 0.3950055020325287 1103 112-113 0.5181265951896243 1103 114-115 0.4859471775911537 1103 116-117 0.3140536833597949 1103 118-119 0.17278288037638134 1103 120-121 0.06722657521934394 1103 122-123 -0.15859809055471885 1103 124-125 -0.30211806170484934 1104 1 -0.11201494112208366 1104 2 0.24976950994618363 1104 3 -0.018904622060794907 1104 4 0.197385698867226 1104 5 0.02092190119871873 1104 6 0.11683151914166245 1104 7 -0.21748222392851346 1104 8 0.04698366840254664 1104 9 -0.16536543398915882 1104 10-11 0.0290767117314914 1104 12-13 -0.0322421518937972 1104 14-15 -0.1497478718708649 1104 16-17 -0.20522016628234496 1104 18-19 -0.21601780993789532 1104 20-21 -0.35271915546742605 1104 22-23 -0.590361820513003 1104 24-25 -0.4721224594444564 1104 26-27 -0.2843343391599831 1104 28-29 -0.3043090783681279 1104 30-31 -0.43522792940282073 1104 32-33 -0.4154262562452544 1104 34-35 -0.2246583830411879 1104 36-37 -0.31041273103022604 1104 38-39 -0.11693414238724387 1104 40-41 -0.23246862060135953 1104 42-43 -0.1009960869909392 1104 44-45 -0.10166146046045554 1104 46-47 -0.22247465828931468 1104 48-49 -0.14604187391719137 1104 50-51 -0.2729708552207981 1104 52-53 -0.18196298724298998 1104 54-55 -0.2911470393029276 1104 56-57 -0.3947944843242581 1104 58-59 -0.2722645444145684 1104 60-61 -0.1343767025345599 1104 62-63 -0.4020532700140187 1104 64-65 -0.39192428943426805 1104 66-67 -0.3477548533510806 1104 68-69 -0.16072442903573503 1104 70-71 -0.017791475521097055 1104 72-73 -0.18720648392599415 1104 74-75 -0.3328382346361707 1104 76-77 -0.2679938280406482 1104 78-79 -0.4343867396206882 1104 80-81 -0.5107056553474756 1104 82-83 -0.6589565187629027 1104 84-85 -0.6595450969543215 1104 86-87 -0.6476051020065157 1104 88-89 -0.5243705801324516 1104 90-91 -0.5941262091912911 1104 92-93 -0.8546768415275849 1104 94-95 -0.7405253793135032 1104 96-97 -0.7757406045591217 1104 98-99 -0.6777476097974606 1104 100-101 -0.6484757795037766 1104 102-103 -0.6703625149567145 1104 104-105 -0.8527809545899885 1104 106-107 -0.8203826452865677 1104 108-109 -0.607940319020237 1104 110-111 -0.6384991228390042 1104 112-113 -0.6562182146767661 1104 114-115 -0.6484053403944614 1104 116-117 -0.569097652714202 1104 118-119 -0.6691364413091279 1104 120-121 -0.5817456755514705 1104 122-123 -0.26954361984557096 1104 124-125 -0.2782293155589066 1105 1 -0.35456751870608016 1105 2 -0.26769330103099165 1105 3 -0.3352806159053685 1105 4 -0.7856131469906877 1105 5 -0.4322840490449309 1105 6 -0.5280165188167913 1105 7 -0.5866570123357953 1105 8 -0.6434978653240471 1105 9 -0.5906187040584072 1105 10-11 -0.47717970016899613 1105 12-13 -0.23123708649241337 1105 14-15 -0.5601656071876064 1105 16-17 -0.47901953791609486 1105 18-19 -0.6388634113741603 1105 20-21 -0.5034437002224905 1105 22-23 -0.4679265807079318 1105 24-25 -0.3136411180748766 1105 26-27 -0.4723889685521385 1105 28-29 -0.2993190168138824 1105 30-31 -0.37115701378343147 1105 32-33 -0.09744728727885388 1105 34-35 -0.24799289188960927 1105 36-37 -0.2343579734000656 1105 38-39 -0.24113586077657345 1105 40-41 -0.4845380614836401 1105 42-43 -0.17512086257442405 1105 44-45 -0.04860138095288846 1105 46-47 -0.22298761032830328 1105 48-49 -0.11247236891590973 1105 50-51 -0.039314789562929775 1105 52-53 -0.2633444312029809 1105 54-55 -0.38007689311159254 1105 56-57 -0.3043706577020444 1105 58-59 -0.05017715021092073 1105 60-61 -0.24528013931323045 1105 62-63 -0.28856904328921473 1105 64-65 -0.06227245563073325 1105 66-67 0.01669276142193965 1105 68-69 -0.171722441346585 1105 70-71 -0.5825981567214029 1105 72-73 -0.3148113184989647 1105 74-75 -0.3543597786218058 1105 76-77 -0.45527422044895616 1105 78-79 -0.1766796352349438 1105 80-81 -0.2066308925877962 1105 82-83 -0.2596377831896781 1105 84-85 -0.21625194486404098 1105 86-87 -0.2319440350662738 1105 88-89 -0.17388006974517012 1105 90-91 -0.04401560390789072 1105 92-93 0.018075787351616412 1105 94-95 0.21639626726254235 1105 96-97 -0.0036724460569388384 1105 98-99 0.03597225427025208 1105 100-101 -0.07773969332782826 1105 102-103 0.03381964713613428 1105 104-105 -0.0755335834691877 1105 106-107 -0.08991810309626302 1105 108-109 -0.19506522284173045 1105 110-111 -0.034196737612024464 1105 112-113 -0.021654865099950626 1105 114-115 -0.14521862585226586 1105 116-117 -0.10024826825664945 1105 118-119 -0.2939921735481654 1105 120-121 -0.1468328773980936 1105 122-123 -0.23809965985582693 1105 124-125 -0.3654584127633207 1106 1 -0.4114197030268443 1106 2 -0.674635251958577 1106 3 -0.4749196596547769 1106 4 -0.28723209561898955 1106 5 -0.3408575474228357 1106 6 -0.5493652227129786 1106 7 -0.5898193166603392 1106 8 -0.3290501661839187 1106 9 -0.4784293437635938 1106 10-11 -0.4259828120780256 1106 12-13 -0.34779666317199087 1106 14-15 -0.36289010244730235 1106 16-17 -0.5531368329490078 1106 18-19 -0.6761055292361391 1106 20-21 -0.7059146441892281 1106 22-23 -0.6830309934453354 1106 24-25 -3.765245893028414 1106 26-27 -1.3206125346487028 1106 28-29 -0.9255340157114844 1106 30-31 -0.8731665258940495 1106 32-33 -5.43685482767382 1106 34-35 -1.526428433166501 1106 36-37 -0.7312460643635603 1106 38-39 -0.43137649827696833 1106 40-41 -0.586838921353241 1106 42-43 -0.3077943326049777 1106 44-45 -0.4163074504353901 1106 46-47 -0.6351312998933238 1106 48-49 -0.4972010217868714 1106 50-51 -0.5261663439425988 1106 52-53 -0.4695099797241937 1106 54-55 -0.29825856812497875 1106 56-57 0.00160275878351257 1106 58-59 -0.22491178752233765 1106 60-61 -0.21435790554208012 1106 62-63 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70-71 -2.028813643008778 2216 72-73 -2.2657163361427415 2216 74-75 -1.73930375187755 2216 76-77 -1.721842350208135 2216 78-79 -1.904491419423639 2216 80-81 -1.6597820100272784 2216 82-83 -1.4747816419156088 2216 84-85 -1.6008012545897898 2216 86-87 -1.7117368754055313 2216 88-89 -1.6242782156004303 2216 90-91 -1.4601360614080434 2216 92-93 -1.2362655114783223 2216 94-95 -1.2638628177371451 2216 96-97 -1.3956913434753737 2216 98-99 -1.3892931762999225 2216 100-101 -1.4387775036417025 2216 102-103 -1.433632219390212 2216 104-105 -1.3998560777426974 2216 106-107 -1.3703826452865684 2216 108-109 -1.3987038658182698 2216 110-111 -1.3064178420508306 2216 112-113 -1.3976898895536145 2216 114-115 -1.4236208576358393 2216 116-117 -1.3540730221723294 2216 118-119 -1.4297583624913948 2216 120-121 -1.4850092223495004 2216 122-123 -1.3170497774810386 2216 124-125 -1.413512566790434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15293.0 3 2818.0 4 3479.0 5 4540.0 6 5146.0 7 2647.0 8 1711.0 9 1263.0 10 1325.0 11 1282.0 12 1398.0 13 1444.0 14 1525.0 15 1486.0 16 1418.0 17 1344.0 18 1198.0 19 1090.0 20 1072.0 21 1099.0 22 1454.0 23 2245.0 24 2905.0 25 3383.0 26 5047.0 27 3936.0 28 4555.0 29 5468.0 30 6531.0 31 7970.0 32 10498.0 33 13933.0 34 19057.0 35 28713.0 36 49545.0 37 89559.0 38 35956.0 39 51.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.58707794817434 24.190842851401644 10.032849712565014 21.189229487859002 2 16.316474607071406 27.401764210808338 36.93077409190321 19.350987090217046 3 13.279923583869907 38.44657423343613 28.866892070952844 19.406610111741116 4 8.229610171762062 22.262087121631303 40.30409281791125 29.204209888695388 5 7.777366831595225 48.57024704337165 35.068335172883764 8.584050952149362 6 22.9671996994592 48.28368488646722 17.724917802673996 11.024197611399583 7 23.48564272817287 40.04135360242297 24.538994699749548 11.93400896965461 8 22.6729066133922 40.51965903564283 20.77442126629759 16.03301308466738 9 25.353548296910677 20.22680676576115 19.692407157168816 34.727237780159356 10-11 18.44565334513759 34.67087387405753 30.299319410913306 16.584153369891578 12-13 20.416335398119266 34.282581535746424 28.450216525859336 16.850866540274975 14-15 19.80129538054716 36.043206532818445 24.13490829241585 20.020589794218548 16-17 18.302676948884024 37.02704906574717 26.529955500675644 18.140318484693164 18-19 17.95364265411677 36.420862261777174 27.289618377522018 18.33587670658404 20-21 18.773671312226156 36.14158884832741 27.677797856276452 17.40694198316998 22-23 19.078681782476327 35.56514502986873 27.378386374202275 17.977786813452663 24-25 19.140242765947534 36.33728041563767 27.04746400447323 17.475012813941568 26-27 17.486072240876698 36.77976812792861 27.59982410106617 18.134335530128517 28-29 18.329455208384903 33.69440181257444 29.58887992567979 18.387263053360865 30-31 18.393815579869475 36.139076647086746 27.714857344217325 17.752250428826457 32-33 17.841853584096825 36.9748498738999 28.179731957201685 17.003564584801588 34-35 17.862400456636294 35.96724333501757 28.199766731075293 17.970589477270842 36-37 17.9760179974227 36.02003596572336 26.96206125821788 19.04188477863607 38-39 17.20004077115732 37.31449123420118 28.315568757644595 17.169899236996912 40-41 16.797109911599524 36.85430593758236 29.900795182021113 16.447788968797006 42-43 17.385163332644105 36.34919433046284 27.814746970548576 18.450895366344476 44-45 16.92520061681206 37.084587415927324 27.80008066899937 18.190131298261246 46-47 17.623486721591718 35.46437297780574 28.5833063950119 18.328833905590646 48-49 17.304438484141617 37.2154416497031 27.79432878156461 17.685791084590676 50-51 17.61709711849426 35.8744479504455 27.92973954473112 18.57871538632912 52-53 17.23001160520181 35.96229255883779 28.082695435529974 18.725000400430428 54-55 18.448446830538312 36.3788064774003 27.892981068995294 17.279765623066094 56-57 18.25376370390109 36.565044113128806 26.737113709798372 18.444078473171732 58-59 17.140365106245422 35.049995704461104 28.647021735426836 19.16261745386664 60-61 18.233271836927255 36.71248076113815 27.31124801786939 17.7429993840652 62-63 17.57414172948589 36.52372957616864 28.863109183862168 17.0390195104833 64-65 18.288048226454656 34.824975246083056 28.5315102801561 18.35546624730619 66-67 18.451144132272322 35.72080697181714 27.466163826054036 18.361885069856502 68-69 18.178800087948314 35.884077132419 28.079974722140115 17.85714805749257 70-71 19.08859122751583 34.01008502230034 28.808566590123785 18.09275716006005 72-73 18.33564853239677 35.60543183558883 27.06977340171179 18.989146230302605 74-75 18.39680473407245 35.32469574789666 27.85635864326423 18.42214087476666 76-77 17.386125696379846 34.83209710439946 29.948483180588436 17.833294018632255 78-79 17.44031801271177 34.483702576572774 28.74374786134995 19.332231549365503 80-81 17.660018113884497 36.037165497418336 27.292119877804083 19.010696510893084 82-83 19.682832289309605 33.76754236523066 27.442826231933438 19.106799113526296 84-85 17.1163103589869 34.096475364243425 28.06458094896952 20.722633327800153 86-87 18.000599913216437 35.27416907651224 28.71968035691924 18.00555065335209 88-89 18.662715776138857 33.84214396482057 29.08782480178809 18.407315457252484 90-91 18.651740692258073 34.565289246571254 28.436136273292107 18.346833787878566 92-93 18.69038171718128 34.78482450328715 27.803251476123563 18.721542303408008 94-95 17.9474783950842 33.48758308882952 29.009340895357216 19.55559762072907 96-97 18.953499377516327 34.450503447321864 28.529846454027215 18.066150721134594 98-99 18.044137304409364 34.76831992265196 28.769042148271755 18.418500624666915 100-101 17.593619952065186 34.46020915420973 28.75215138780895 19.194019505916135 102-103 18.851882538124727 34.51298053611237 27.79257352288066 18.842563402882234 104-105 16.927478831914847 37.053140448333856 27.846060879631313 18.173319840119984 106-107 17.214476567675987 35.434682897352076 28.828929837717414 18.52191069725452 108-109 18.233218737759604 35.864046257588136 27.12012732194367 18.78260768270859 110-111 17.847111294904632 36.87119682101925 26.245375028576174 19.036316855499948 112-113 16.78871229605469 35.850400064068495 29.173589219019608 18.187298420857207 114-115 17.503126988495303 36.416201804698275 27.550115542042885 18.530555664763533 116-117 17.68455784601092 36.46629186487915 27.44280050031816 18.40634978879177 118-119 17.382542322040663 34.110851275121654 28.22391584808622 20.282690554751458 120-121 17.392925059918866 35.28704267271631 28.090080697534226 19.229951569830597 122-123 17.46264723055843 35.47453779542579 28.29884545323496 18.763969520780822 124-125 17.822955708348985 35.0749745910543 27.42302327139084 19.679046429205872 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2478.0 1 3268.5 2 3703.0 3 2732.0 4 1641.0 5 1044.0 6 809.0 7 677.5 8 656.0 9 637.5 10 538.0 11 414.0 12 371.0 13 364.5 14 362.0 15 331.5 16 315.0 17 325.0 18 349.0 19 368.5 20 423.5 21 514.5 22 565.0 23 745.0 24 841.0 25 947.0 26 1399.5 27 1944.5 28 2347.5 29 3309.5 30 5581.5 31 7313.5 32 9391.5 33 13166.0 34 16594.5 35 19214.5 36 24540.5 37 28731.5 38 26502.0 39 24336.0 40 22007.0 41 17676.5 42 12964.0 43 11382.0 44 12692.0 45 13050.5 46 10058.5 47 6458.0 48 5474.5 49 4595.5 50 4536.0 51 3479.5 52 1867.0 53 1239.0 54 913.0 55 820.0 56 726.0 57 600.0 58 551.0 59 518.5 60 448.0 61 403.5 62 371.0 63 304.5 64 260.0 65 224.5 66 172.5 67 149.5 68 152.5 69 122.0 70 95.5 71 93.5 72 77.5 73 73.5 74 71.5 75 60.0 76 39.5 77 23.5 78 16.5 79 10.5 80 11.5 81 7.5 82 3.0 83 2.0 84 5.0 85 3.5 86 3.0 87 3.0 88 0.5 89 2.5 90 3.0 91 2.0 92 1.0 93 0.5 94 0.5 95 0.0 96 1.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.824383197819351E-4 2 2.9121915989096755E-4 3 2.9121915989096755E-4 4 5.824383197819351E-4 5 8.736574796729026E-4 6 8.736574796729026E-4 7 0.0011648766395638702 8 0.0014560957994548377 9 0.0023297532791277404 10-11 0.0014560957994548377 12-13 0.002038534119236773 14-15 0.0029121915989096755 16-17 0.0023297532791277404 18-19 0.0023297532791277404 20-21 0.002766582018964192 22-23 0.08722013838734477 24-25 0.0023297532791277404 26-27 0.0014560957994548377 28-29 0.0014560957994548377 30-31 0.0014560957994548377 32-33 0.0017473149593458053 34-35 0.0010192670596183865 36-37 4.368287398364513E-4 38-39 0.0011648766395638702 40-41 0.0010192670596183865 42-43 0.0014560957994548377 44-45 0.0016017053794003214 46-47 0.002038534119236773 48-49 2.9121915989096755E-4 50-51 0.0013104862195093539 52-53 0.0010192670596183865 54-55 0.0016017053794003214 56-57 0.0016017053794003214 58-59 0.0013104862195093539 60-61 0.0010192670596183865 62-63 0.0013104862195093539 64-65 0.0011648766395638702 66-67 4.368287398364513E-4 68-69 1.4560957994548377E-4 70-71 0.0010192670596183865 72-73 2.9121915989096755E-4 74-75 2.9121915989096755E-4 76-77 2.9121915989096755E-4 78-79 4.368287398364513E-4 80-81 2.9121915989096755E-4 82-83 2.9121915989096755E-4 84-85 2.9121915989096755E-4 86-87 2.9121915989096755E-4 88-89 4.368287398364513E-4 90-91 1.4560957994548377E-4 92-93 4.368287398364513E-4 94-95 4.368287398364513E-4 96-97 4.368287398364513E-4 98-99 2.9121915989096755E-4 100-101 2.9121915989096755E-4 102-103 0.0013104862195093539 104-105 4.368287398364513E-4 106-107 4.368287398364513E-4 108-109 7.280478997274189E-4 110-111 0.0010192670596183865 112-113 4.368287398364513E-4 114-115 7.280478997274189E-4 116-117 7.280478997274189E-4 118-119 0.0014560957994548377 120-121 8.736574796729026E-4 122-123 7.280478997274189E-4 124-125 2.9121915989096755E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 343384.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.81663676816625 #Duplication Level Percentage of deduplicated Percentage of total 1 72.07929379835201 15.004484775062322 2 8.03430282172885 3.3449432705076534 3 4.5718442663085295 2.855112643571046 4 2.9140610791678907 2.4264380402115417 5 2.0438997775632686 2.127355963003518 6 1.3947762342440648 1.7420730144677679 7 1.0464319189714748 1.5248235211891061 8 0.7722331808452596 1.2860238100785126 9 0.5987605097858172 1.121776203900007 >10 5.022313621801597 21.97161195629383 >50 0.8491767042990446 12.61736132143606 >100 0.6225430533988053 24.5518137129278 >500 0.04057022145745023 5.882627029797544 >1k 0.009792812075936262 3.543554737553293 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3408 0.9924748969084175 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2171 0.6322367961232905 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 1643 0.4784730797008597 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1426 0.41527852200451976 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1343 0.39110733173356943 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1090 0.3174288842811546 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1087 0.31655522680148174 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 973 0.2833562425739114 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 918 0.2673391887799082 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 890 0.2591850523029611 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 862 0.25103091582601406 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 851 0.24782750506721338 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 810 0.2358875195116837 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 789 0.22977191715397338 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 753 0.21928802739789857 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 746 0.2172494932786618 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 746 0.2172494932786618 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 736 0.21433730167975212 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 700 0.20385341192367726 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 692 0.20152365864454955 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 678 0.19744659040607598 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 676 0.19686415208629407 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 667 0.19424317964727536 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 665 0.19366074132749342 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 659 0.1919134263681476 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 636 0.18521538569065535 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 623 0.18142953661207278 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 621 0.18084709829229084 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 615 0.17909978333294505 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 603 0.17560515341425342 No Hit CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC 572 0.16657735945763344 No Hit GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 557 0.16220907205926893 No Hit CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC 555 0.161626633739487 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 551 0.1604617570999231 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 548 0.15958809962025022 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 508 0.14793933322461153 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 498 0.14502714162570185 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 480 0.13978519674766443 No Hit GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG 474 0.1380378817883186 No Hit GGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTC 474 0.1380378817883186 No Hit GAGTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCA 473 0.13774666262842766 No Hit GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA 457 0.13308715607017219 No Hit GTACATGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACAT 453 0.1319222794306083 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 453 0.1319222794306083 No Hit TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA 451 0.13133984111082636 No Hit GGCTAATATTTATTAATACTAGAGTAGCTCCTCCGATTAGGTGTATTAAT 449 0.13075740279104442 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 425 0.1237681429536612 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 421 0.12260326631409733 No Hit GGTCTAGTAATATGATTTCACTATAATTCAATTACACTATTAACCCTTGG 416 0.1211471705146425 No Hit CCTTCTAACATCAGGTCTAGTAATATGATTTCACTATAATTCAATTACAC 407 0.11852619807562378 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 406 0.11823497891573283 No Hit GGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATG 398 0.11590522563660509 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 396 0.11532278731682315 No Hit TTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAATGGTAGCTGTTG 396 0.11532278731682315 No Hit CAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGT 387 0.11270181487780444 No Hit GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA 384 0.11182815739813154 No Hit CCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGA 381 0.11095449991845863 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 381 0.11095449991845863 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 377 0.10978962327889477 No Hit GTCCATGACCATTAACTGGAGCCTTTTCAGCCCTCCTTCTAACATCAGGT 377 0.10978962327889477 No Hit GGTTAATAGTGTAATTGAATTATAGTGAAATCATATTACTAGACCTGATG 371 0.10804230831954896 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 369 0.10745986999976702 No Hit GTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGA 367 0.10687743167998509 No Hit GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA 364 0.10600377420031219 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 363 0.10571255504042121 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 357 0.10396524008107542 No Hit ATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATT 353 0.10280036344151156 No Hit GCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGT 349 0.10163548680194767 No Hit GTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATT 349 0.10163548680194767 No Hit GGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAA 347 0.10105304848216573 No Hit GTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAA 345 0.1004706101623838 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 344 0.10017939100249285 No Hit GTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGAATA 344 0.10017939100249285 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.4560957994548377E-4 0.0 20-21 0.0 0.0 0.0 5.824383197819351E-4 0.0 22-23 0.0 0.0 0.0 8.736574796729026E-4 0.0 24-25 0.0 0.0 0.0 0.0014560957994548377 0.0 26-27 0.0 0.0 0.0 0.0014560957994548377 0.0 28-29 0.0 0.0 0.0 0.0023297532791277404 0.0 30-31 0.0 0.0 0.0 0.00815413647694709 0.0 32-33 0.0 0.0 0.0 0.020530950772313213 0.0 34-35 0.0 0.0 0.0 0.03188849800806095 0.0 36-37 0.0 0.0 0.0 0.047905551802064156 0.0 38-39 0.0 0.0 0.0 0.0780467348507793 0.0 40-41 0.0 0.0 0.0 0.11168254781818605 0.0 42-43 0.0 0.0 0.0 0.1482305523845025 0.0 44-45 0.0 0.0 0.0 0.18710831022994667 0.0 46-47 0.0 0.0 0.0 0.23836288237075695 0.0 48-49 0.0 0.0 0.0 0.29136476947091305 0.0 50-51 0.0 0.0 0.0 0.3418912937119959 0.0 52-53 0.0 0.0 0.0 0.39547561913193396 0.0 54-55 0.0 0.0 0.0 0.45910700556811035 0.0 56-57 0.0 0.0 0.0 0.5256505836031964 0.0 58-59 0.0 0.0 0.0 0.6137443794702141 0.0 60-61 0.0 0.0 0.0 0.7048959765160869 0.0 62-63 0.0 0.0 0.0 0.8011439088600517 0.0 64-65 0.0 0.0 0.0 0.9128264566782378 0.0 66-67 0.0 0.0 0.0 1.0415453253500455 0.0 68-69 0.0 0.0 0.0 1.1874461244554202 0.0 70-71 0.0 0.0 0.0 1.3588286000512544 0.0 72-73 0.0 0.0 0.0 1.5270076648882882 0.0 74-75 0.0 0.0 0.0 1.7155720709176898 0.0 76-77 0.0 0.0 0.0 1.9086503739254015 0.0 78-79 0.0 0.0 0.0 2.152692029914032 0.0 80-81 0.0 0.0 0.0 2.4096929385178107 0.0 82-83 0.0 0.0 0.0 2.6680043333410994 0.0 84-85 0.0 0.0 0.0 2.9672320201290683 0.0 86-87 0.0 0.0 0.0 3.2727209188546933 0.0 88-89 0.0 0.0 0.0 3.6004880833119772 0.0 90-91 0.0 0.0 0.0 3.9482037602217925 0.0 92-93 0.0 0.0 0.0 4.351105467930946 0.0 94-95 0.0 0.0 0.0 4.783565920369034 0.0 96-97 0.0 0.0 0.0 5.261310952170165 0.0 98-99 0.0 0.0 0.0 5.765556927521375 0.0 100-101 0.0 0.0 0.0 6.304457982899611 0.0 102-103 0.0 0.0 0.0 6.841757332898446 0.0 104-105 0.0 0.0 0.0 7.364786944062624 0.0 106-107 0.0 0.0 0.0 7.94460429140554 0.0 108-109 0.0 0.0 0.0 8.523547981268784 0.0 110-111 0.0 0.0 0.0 9.134671388299978 0.0 112-113 0.0 0.0 0.0 9.766616965263378 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGACAT 35 8.949064E-7 84.99345 7 TGGGTAT 180 0.0 72.71661 6 ATAGGCC 25 0.0020562443 71.3945 4 ACCGACT 25 0.0020562443 71.3945 8 CAGGGTA 25 0.0020562443 71.3945 4 GTATGGT 70 1.7462298E-10 67.99476 1 TACCTGG 55 1.7248203E-7 64.90409 2 TATGGTG 75 3.2196112E-10 63.461773 2 GAACAAA 595 0.0 61.99522 1 ATGGGTA 255 0.0 60.661987 5 CATGGGT 340 0.0 59.49541 4 GGGACCG 30 0.004235319 59.49541 7 GGTATGG 150 0.0 59.49541 8 ACATCGG 30 0.004235319 59.49541 8 GGATGAC 475 0.0 57.61661 8 GATGACC 425 0.0 55.995686 9 GGGATGA 495 0.0 55.288666 7 GATGGCC 110 3.6379788E-12 54.08674 9 CGCAGAA 45 3.169E-4 52.884808 2 GGTTACA 45 3.169E-4 52.884808 1 >>END_MODULE