FastQCFastQC Report
Thu 26 May 2016
SRR936188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343384
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54301.5813200382079537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53401.5551103138177667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48431.410374391351956No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG14060.4094541388067004No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13640.3972229340912797No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT13600.39605805745171585No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13500.39314586585280614No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG13210.38470051021596813No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12630.36780979894229204No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT10520.3063625562052979No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG9880.28772452997227593No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC9550.278114297695874No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC9340.2719986953381637No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA8940.26034992894252496No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG8920.25976749062274307No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC8500.2475362859073224No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8120.23646995783146565No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA8040.23414020455233792No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA7690.22394753395615402No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7480.21783193159844372No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT7360.21433730167975212No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.21171632924073341No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT7120.20734804184236888No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG6900.2009412203247676No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA6890.2006500011648766No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG6880.2003587820049857No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC6830.19890268620553084No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT6470.188418796449456No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC6260.1823031940917457No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG6150.17909978333294505No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC5810.16919833189665215No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5720.16657735945763344No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC5440.15842322298068634No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT5350.15580225054166763No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG5350.15580225054166763No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT5260.15318127810264895No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG5260.15318127810264895No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT5230.152307620622976No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT5090.14823055238450247No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG4850.14124129254711926No Hit
GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT4750.1383291009482096No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA4460.12988374531137153No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4440.1293013069915896No Hit
GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA4410.12842764951191668No Hit
GAGTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCA4340.1263891153926799No Hit
GGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATG4320.12580667707289797No Hit
GGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTC4280.12464180043333412No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA4260.12405936211355219No Hit
GGTTAATAGTGTAATTGAATTATAGTGAAATCATATTACTAGACCTGATG4250.1237681429536612No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT4250.1237681429536612No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC4200.12231204715420638No Hit
TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA4180.12172960883442444No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG4100.11939985555529668No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC4070.11852619807562378No Hit
CCTTCTAACATCAGGTCTAGTAATATGATTTCACTATAATTCAATTACAC4050.11794375975584186No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT4040.1176525405959509No Hit
GTACATGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACAT4010.11677888311627797No Hit
ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT4000.11648766395638702No Hit
TTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAATGGTAGCTGTTG4000.11648766395638702No Hit
CAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGT3970.11561400647671413No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT3740.10891596579922186No Hit
GCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGT3690.10745986999976702No Hit
GTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGA3660.10658621252009413No Hit
GGTCTAGTAATATGATTTCACTATAATTCAATTACACTATTAACCCTTGG3630.10571255504042121No Hit
GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG3610.10513011672063928No Hit
GGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATAC3600.10483889756074832No Hit
CCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGA3600.10483889756074832No Hit
GCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTG3590.10454767840085734No Hit
GAATAGAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGT3590.10454767840085734No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA3580.10425645924096638No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA3530.10280036344151156No Hit
ACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCAT3460.10076182932227477No Hit
GGCTAATATTTATTAATACTAGAGTAGCTCCTCCGATTAGGTGTATTAAT3460.10076182932227477No Hit
ATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATT3440.10017939100249285No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC251.7170072E-595.277775
TACCTGG454.0199666E-1092.6311652
CAGCATA208.4784295E-489.2448659
TAACGCA358.9015884E-785.0694354
TATAACG358.9015884E-785.0694352
CCGAGTA304.2427273E-579.398144
TGGGTAT1700.069.995976
GGTATCA21000.065.0026551
CATGGGT2950.060.5579034
ACCTGGG701.3293175E-859.5486073
CAGCACA401.7630891E-459.5486034
CTGTCAC150.004234348459.505245112-113
GTACAGC300.004234989659.496586
AAGCGTA150.004236791359.4965822-23
TACGCAC150.004236791359.4965886-87
ACGTGCT150.004236791359.4965864-65
ACTAAGC150.004236791359.4965818-19
TAAGCGA150.004236791359.4965812-13
GTACGCA150.004236791359.4965884-85
ACGCACA201.7719818E-459.49657486-87
Produced by FastQC (version 0.11.5)