##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936188_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 343384 Sequences flagged as poor quality 0 Sequence length 125 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.439924399506094 31.0 31.0 34.0 30.0 34.0 2 31.411740209211846 31.0 31.0 34.0 30.0 34.0 3 31.70870221093586 31.0 31.0 34.0 30.0 34.0 4 35.56199764694919 37.0 35.0 37.0 33.0 37.0 5 35.25730668872166 37.0 35.0 37.0 33.0 37.0 6 35.45408929944319 37.0 35.0 37.0 33.0 37.0 7 35.281431283927034 37.0 35.0 37.0 32.0 37.0 8 35.36107389977401 37.0 35.0 37.0 33.0 37.0 9 36.99458623581763 39.0 37.0 39.0 33.0 39.0 10-11 36.865397048202595 39.0 37.0 39.0 32.0 39.0 12-13 36.86676723434988 39.0 37.0 39.0 32.0 39.0 14-15 37.86866598327237 40.0 37.0 41.0 32.0 41.0 16-17 37.81992172028982 40.0 37.0 41.0 32.0 41.0 18-19 38.0542177270927 40.0 37.0 41.0 33.0 41.0 20-21 38.138356766768396 40.0 37.0 41.0 33.0 41.0 22-23 37.9975916175477 40.0 37.0 41.0 33.0 41.0 24-25 37.97119260070359 40.0 37.0 41.0 33.0 41.0 26-27 37.76177253453859 40.0 37.0 41.0 32.0 41.0 28-29 37.602924131584466 40.0 37.0 41.0 32.0 41.0 30-31 37.32199811289984 39.0 36.0 41.0 32.0 41.0 32-33 36.945228956503506 39.0 35.0 41.0 31.0 41.0 34-35 36.68126208559514 39.0 35.0 41.0 31.0 41.0 36-37 36.472273023786784 39.0 35.0 41.0 30.0 41.0 38-39 36.424115567411405 39.0 35.0 41.0 30.0 41.0 40-41 36.41525959275913 39.0 35.0 41.0 30.0 41.0 42-43 36.26402365864455 39.0 35.0 41.0 30.0 41.0 44-45 36.02140169606039 39.0 35.0 41.0 29.5 41.0 46-47 35.5849195070242 39.0 35.0 41.0 26.0 41.0 48-49 35.22072373785616 39.0 35.0 41.0 24.0 41.0 50-51 35.088692251240595 39.0 35.0 41.0 24.0 41.0 52-53 35.06814965170189 39.0 35.0 41.0 24.0 41.0 54-55 34.955379400321505 39.0 35.0 41.0 24.0 41.0 56-57 34.84180247186823 39.0 35.0 41.0 23.5 41.0 58-59 34.689117722433195 39.0 35.0 41.0 23.0 41.0 60-61 34.55568692775435 39.0 34.5 40.5 23.0 41.0 62-63 34.35593388160194 38.0 34.0 40.0 22.5 41.0 64-65 34.1396832700417 38.0 34.0 40.0 22.0 41.0 66-67 33.85726184097104 37.5 34.0 40.0 22.0 41.0 68-69 33.56796618363116 37.0 34.0 40.0 21.0 41.0 70-71 33.2500087365748 37.0 33.0 39.5 20.0 41.0 72-73 32.937322356312464 36.0 33.0 39.0 20.0 41.0 74-75 32.654471378980965 36.0 33.0 39.0 18.5 40.0 76-77 32.301828273885796 35.5 33.0 38.5 15.5 40.0 78-79 31.912153740418887 35.0 32.5 37.0 12.5 39.0 80-81 31.55763664002982 35.0 32.0 37.0 9.0 39.0 82-83 31.187872760524662 35.0 32.0 36.5 6.5 39.0 84-85 30.765989387973814 35.0 32.0 36.0 2.0 37.0 86-87 30.520528038580714 35.0 32.0 36.0 2.0 37.0 88-89 30.279940241828392 35.0 31.5 35.5 2.0 36.5 90-91 30.042084080795846 35.0 31.0 35.0 2.0 36.0 92-93 29.82459724390187 35.0 31.0 35.0 2.0 36.0 94-95 29.631734443072478 35.0 31.0 35.0 2.0 36.0 96-97 29.508806467395104 34.5 31.0 35.0 2.0 36.0 98-99 29.41011957458705 34.0 31.0 35.0 2.0 35.5 100-101 29.289001525988397 34.0 31.0 35.0 2.0 35.0 102-103 28.277288691377585 33.5 29.0 34.5 2.0 35.0 104-105 28.93197120424947 34.0 30.5 35.0 2.0 35.0 106-107 29.024472602101437 34.0 31.0 35.0 2.0 35.0 108-109 28.960801901078675 34.0 31.0 35.0 2.0 35.0 110-111 28.870184691191202 34.0 31.0 35.0 2.0 35.0 112-113 28.78037270228083 34.0 31.0 35.0 2.0 35.0 114-115 28.72128870302635 34.0 31.0 35.0 2.0 35.0 116-117 28.593367774852645 34.0 30.5 35.0 2.0 35.0 118-119 28.45929484192624 34.0 30.0 35.0 2.0 35.0 120-121 28.269039908673673 34.0 30.0 35.0 2.0 35.0 122-123 28.06859376092072 34.0 30.0 35.0 2.0 35.0 124-125 26.149456585047645 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34594606231682334 1101 2 0.7254432417410186 1101 3 0.2835468700296495 1101 4 0.45157282039749447 1101 5 0.6299699202776878 1101 6 0.34430889966301237 1101 7 0.5645200735579721 1101 8 0.5623298674257384 1101 9 0.46787720874561245 1101 10-11 0.6072160528392345 1101 12-13 0.47342250900560856 1101 14-15 0.6162047064254068 1101 16-17 0.7923890872305606 1101 18-19 0.8665434454787189 1101 20-21 0.730581487409836 1101 22-23 0.6505000601979489 1101 24-25 0.5369529833621982 1101 26-27 0.7143701189348164 1101 28-29 0.6576831492614303 1101 30-31 0.44650460682350257 1101 32-33 0.5027367475670133 1101 34-35 0.4928686585959241 1101 36-37 0.575700890587818 1101 38-39 0.6313724355567558 1101 40-41 0.9045142437730647 1101 42-43 0.7052013795404477 1101 44-45 0.7454950324476002 1101 46-47 0.6336543288869692 1101 48-49 0.5063524564337243 1101 50-51 0.23092270589626196 1101 52-53 0.18633737208224943 1101 54-55 0.23750418942324814 1101 56-57 0.28825738177356186 1101 58-59 0.3628109139940676 1101 60-61 0.28533097147895603 1101 62-63 0.11105228983750948 1101 64-65 0.3046496170480779 1101 66-67 0.15171472457809188 1101 68-69 0.22802581764806007 1101 70-71 0.04739643749992695 1101 72-73 0.26716020083648573 1101 74-75 -0.006977758799941114 1101 76-77 0.3126990781873147 1101 78-79 0.1496476402469682 1101 80-81 0.25589026060125164 1101 82-83 0.20137702998664508 1101 84-85 -0.04251730158751954 1101 86-87 0.02948972250513293 1101 88-89 0.1756900074199308 1101 90-91 0.3851666734041288 1101 92-93 0.4795523910724917 1101 94-95 0.5114156493368966 1101 96-97 0.49380759040201383 1101 98-99 0.4703338323917059 1101 100-101 0.3437119658973913 1101 102-103 0.3956371471955933 1101 104-105 0.49146893350368615 1101 106-107 0.5053386105927764 1101 108-109 0.47017189286642136 1101 110-111 0.5926674753264152 1101 112-113 0.5292475030087758 1101 114-115 0.4107498294540939 1101 116-117 0.6853252724599983 1101 118-119 0.6502701020384407 1101 120-121 0.42497670198737225 1101 122-123 0.5995131383157677 1101 124-125 0.5818386501651354 1102 1 0.3186527712423981 1102 2 0.6742496868724928 1102 3 0.5473993008890226 1102 4 0.30866706683433165 1102 5 0.5255994937349513 1102 6 0.4419516044923242 1102 7 0.4888720928466981 1102 8 0.476690766270103 1102 9 0.4629027131627055 1102 10-11 0.5832704190528517 1102 12-13 0.3824767391092436 1102 14-15 0.48153360646234944 1102 16-17 0.7109450900046141 1102 18-19 0.6253403629118068 1102 20-21 0.5341407633047623 1102 22-23 0.41746519659959347 1102 24-25 0.2604423246547256 1102 26-27 0.27489206828894197 1102 28-29 0.2603943303719589 1102 30-31 0.3763998669556017 1102 32-33 0.23897829740595 1102 34-35 0.49612719625625346 1102 36-37 0.40423472687992046 1102 38-39 0.1766209010787989 1102 40-41 0.1366093003883293 1102 42-43 0.27938779835732674 1102 44-45 0.6631663203720493 1102 46-47 0.7417791222914687 1102 48-49 0.555561074139689 1102 50-51 0.5267755645298422 1102 52-53 0.65807186688739 1102 54-55 0.711327324230453 1102 56-57 0.45139491118303 1102 58-59 0.5704110358449057 1102 60-61 0.8442860357423143 1102 62-63 0.5635871761208691 1102 64-65 0.6719910003143283 1102 66-67 0.7565858413928623 1102 68-69 0.5452301187233246 1102 70-71 0.5985835109409834 1102 72-73 0.5590529072861585 1102 74-75 0.531393912176874 1102 76-77 0.22964606659203923 1102 78-79 0.3302202095496334 1102 80-81 0.36568888264977417 1102 82-83 0.4561651845298016 1102 84-85 0.31370033178405166 1102 86-87 0.4356321713180584 1102 88-89 0.16317923204676532 1102 90-91 0.06235107649706606 1102 92-93 0.29942866062209816 1102 94-95 0.14200344999411385 1102 96-97 0.08470312923548207 1102 98-99 0.04847068125129894 1102 100-101 0.2603429847129739 1102 102-103 0.46621827198251964 1102 104-105 0.5080986560337735 1102 106-107 0.41176948292810067 1102 108-109 0.3722488662990493 1102 110-111 0.26247277324522855 1102 112-113 0.4554661734087446 1102 114-115 0.43249825932828756 1102 116-117 0.4847510179861949 1102 118-119 0.3594922011135395 1102 120-121 0.408283785539453 1102 122-123 0.6395959061445566 1102 124-125 0.6707330482673726 1103 1 0.07897254435700063 1103 2 0.6018388994084347 1103 3 0.2798852831701062 1103 4 0.16198513570653716 1103 5 0.4639865779193215 1103 6 0.21229193259150492 1103 7 0.5387406970591684 1103 8 0.3282590595559043 1103 9 0.3328046216539917 1103 10-11 0.4145959553688243 1103 12-13 0.3217728609820867 1103 14-15 0.4543535421955056 1103 16-17 0.5139441012838333 1103 18-19 0.20529502486036932 1103 20-21 0.2589039906975259 1103 22-23 0.3128250389246503 1103 24-25 -0.00524873913077073 1103 26-27 0.20535475058433406 1103 28-29 0.22426536735792268 1103 30-31 0.19019674988324908 1103 32-33 0.05373788214322417 1103 34-35 0.1229420192622257 1103 36-37 0.1153981650263276 1103 38-39 0.16915833586876516 1103 40-41 0.09882975910273473 1103 42-43 0.26511151594745996 1103 44-45 0.27047504843481107 1103 46-47 0.31318167475220804 1103 48-49 0.5752102734059577 1103 50-51 0.5743447424605534 1103 52-53 0.4729339013104763 1103 54-55 0.5454352639184847 1103 56-57 0.6085559816033737 1103 58-59 0.650438876140484 1103 60-61 0.6665195689880505 1103 62-63 0.6581758812869296 1103 64-65 0.8076278795993304 1103 66-67 0.8554871904763459 1103 68-69 0.7646545791549784 1103 70-71 0.8015737408185686 1103 72-73 0.7890426948412852 1103 74-75 0.8690794599411973 1103 76-77 0.8882902181785539 1103 78-79 0.8213070868832126 1103 80-81 0.7891410793011282 1103 82-83 0.7797208065782755 1103 84-85 0.7815750374444796 1103 86-87 0.9149909989033702 1103 88-89 0.9654968716060033 1103 90-91 0.9653702524763546 1103 92-93 0.6717405760084141 1103 94-95 0.8281171268645515 1103 96-97 0.9645020026141751 1103 98-99 0.9280386362905197 1103 100-101 0.9713356733830167 1103 102-103 0.9370266162654985 1103 104-105 1.047185650452704 1103 106-107 0.8087603892988469 1103 108-109 0.8128687031601238 1103 110-111 1.0370216693905192 1103 112-113 1.1988150747578281 1103 114-115 1.163467010547926 1103 116-117 0.9968953712197006 1103 118-119 0.8532915558431391 1103 120-121 0.892795994940105 1103 122-123 0.9305676495454946 1103 124-125 1.1146865653813478 1104 1 0.21458405514836443 1104 2 0.4803075530569423 1104 3 0.2818251598401993 1104 4 0.10873487876923349 1104 5 0.2269105244763594 1104 6 0.203954316969714 1104 7 0.3258295973674947 1104 8 0.2947287409536443 1104 9 0.2626992773580028 1104 10-11 0.26185314961342954 1104 12-13 0.2980960881146686 1104 14-15 0.4563640766251069 1104 16-17 0.5349166089919564 1104 18-19 0.407890091664072 1104 20-21 0.40335414485991095 1104 22-23 0.39915597417644477 1104 24-25 0.3386746935516456 1104 26-27 0.22332109179708226 1104 28-29 0.2522908041513432 1104 30-31 0.1902674076427573 1104 32-33 -0.06932866461936271 1104 34-35 0.11283153622008513 1104 36-37 0.02468002525243662 1104 38-39 0.09207072024697993 1104 40-41 0.014265267838602824 1104 42-43 -0.1257093987493576 1104 44-45 -0.06323512628255656 1104 46-47 -0.14548353593637842 1104 48-49 -0.12362066184584108 1104 50-51 -0.18326060183543547 1104 52-53 -0.37589061050864103 1104 54-55 -0.3878702345398892 1104 56-57 -0.4020747994860443 1104 58-59 -0.42454827699415176 1104 60-61 -0.33688485033363946 1104 62-63 -0.4839489902444214 1104 64-65 -0.2563430864849465 1104 66-67 -0.3166736522780198 1104 68-69 -0.2181627384195366 1104 70-71 -0.2772867422235663 1104 72-73 -0.35010170392541795 1104 74-75 -0.21427742597863642 1104 76-77 -0.3927092679468345 1104 78-79 -0.620785667484725 1104 80-81 -0.49676590939362697 1104 82-83 -0.5299785767721872 1104 84-85 -0.6312589810550087 1104 86-87 -0.5586151162045425 1104 88-89 -0.5647253791648126 1104 90-91 -0.556964022960436 1104 92-93 -0.7826915925424593 1104 94-95 -0.63338081763699 1104 96-97 -0.46370329029435453 1104 98-99 -0.4939230800506884 1104 100-101 -0.6606787151061901 1104 102-103 -0.5499209685238107 1104 104-105 -0.713849806895702 1104 106-107 -0.627165869693961 1104 108-109 -0.7673985116394668 1104 110-111 -0.7815484744943717 1104 112-113 -0.7658945860849258 1104 114-115 -0.7276622289176444 1104 116-117 -0.8954478970228479 1104 118-119 -0.8816583413819359 1104 120-121 -0.7564653103014152 1104 122-123 -0.7577481264051755 1104 124-125 -0.43113177994239393 1105 1 0.2055176278593187 1105 2 0.5694970888353481 1105 3 0.3984159523510975 1105 4 0.1912400082896255 1105 5 0.3963193472514277 1105 6 0.3188138963490488 1105 7 0.42238961275605647 1105 8 0.3108546706792126 1105 9 0.42918747946110614 1105 10-11 0.19779345481988742 1105 12-13 0.3277158624157721 1105 14-15 0.24458060650455593 1105 16-17 0.3140987710847938 1105 18-19 0.25868516732449365 1105 20-21 0.34974424488555655 1105 22-23 0.27011647686943974 1105 24-25 0.22459736603177305 1105 26-27 0.02136065322309122 1105 28-29 0.06030407678534999 1105 30-31 -0.044076334342371126 1105 32-33 0.23420589552426208 1105 34-35 -0.003252716152317703 1105 36-37 0.03447260027166976 1105 38-39 -0.16125865908105652 1105 40-41 -0.2431164325974038 1105 42-43 0.008441986221150444 1105 44-45 0.12286387346096461 1105 46-47 0.06302345303515011 1105 48-49 0.0963748498238104 1105 50-51 0.061661172978617174 1105 52-53 0.1022688534564864 1105 54-55 0.1730604528158466 1105 56-57 0.08546206894176578 1105 58-59 0.29631284124128854 1105 60-61 0.037073215837175155 1105 62-63 0.04435634445679426 1105 64-65 -0.03677236322257471 1105 66-67 0.14435161060989543 1105 68-69 0.1457687183642591 1105 70-71 0.09705764095160418 1105 72-73 -0.07559862621317848 1105 74-75 0.13852297155919047 1105 76-77 0.16101925218653435 1105 78-79 0.0795782078679288 1105 80-81 0.06422729399185556 1105 82-83 0.060047712019809296 1105 84-85 -0.044137283183758313 1105 86-87 0.08500023388522138 1105 88-89 0.10085393604421711 1105 90-91 0.2528445818100167 1105 92-93 0.13606642233314759 1105 94-95 0.06086450167565616 1105 96-97 0.10934268303213912 1105 98-99 -0.012176157762926465 1105 100-101 -0.1841526827902129 1105 102-103 -0.11871424885210047 1105 104-105 -0.2827873549849542 1105 106-107 -0.1403503400709809 1105 108-109 -0.0930781730911221 1105 110-111 -0.15368716390191395 1105 112-113 -0.03603115956530445 1105 114-115 -0.08924051822259216 1105 116-117 -0.029905450241621168 1105 118-119 -0.21249477380050763 1105 120-121 -0.08839176580282526 1105 122-123 0.12829797515933095 1105 124-125 0.1853067299319342 1106 1 0.29791738848170013 1106 2 0.6102574277436617 1106 3 0.3505908240006015 1106 4 0.22712460308000715 1106 5 0.21719874502773706 1106 6 0.0011660964183377587 1106 7 0.3118884946105993 1106 8 0.19717234997619926 1106 9 0.1829687009169021 1106 10-11 0.014375079437989768 1106 12-13 0.05220009813972837 1106 14-15 0.025740643041146427 1106 16-17 0.20798991726262983 1106 18-19 0.06377981597484705 1106 20-21 0.14552206465940998 1106 22-23 0.19696299172030507 1106 24-25 0.14973359079476012 1106 26-27 0.1601162045790332 1106 28-29 -0.08813839384364996 1106 30-31 0.11324673095102611 1106 32-33 0.01652659853853322 1106 34-35 0.018439305643646264 1106 36-37 0.07845508790907729 1106 38-39 0.08390969267805559 1106 40-41 0.08918694703659469 1106 42-43 0.05544661628823633 1106 44-45 0.015962868704910704 1106 46-47 0.20312235378793275 1106 48-49 0.019142496048893065 1106 50-51 -0.11057889757478279 1106 52-53 -0.15855038494472495 1106 54-55 -0.02786083604365075 1106 56-57 -0.10299898494969995 1106 58-59 0.08820234957226347 1106 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-1.41423347732443 2216 74-75 -1.1155397412495773 2216 76-77 -1.426853356616789 2216 78-79 -1.2742647315241342 2216 80-81 -1.2992597517581608 2216 82-83 -1.6385376900726811 2216 84-85 -1.9699289317939233 2216 86-87 -1.9318909782735112 2216 88-89 -2.057644098376631 2216 90-91 -1.901730032812651 2216 92-93 -1.7364785383552679 2216 94-95 -1.8613364826616206 2216 96-97 -2.075833832166275 2216 98-99 -1.9440154445827105 2216 100-101 -1.9611097495889531 2216 102-103 -1.733480081824304 2216 104-105 -1.8458387231518607 2216 106-107 -1.7069072490043062 2216 108-109 -1.6486792998168092 2216 110-111 -1.4949105434598877 2216 112-113 -1.3346753742622681 2216 114-115 -1.3866523767008943 2216 116-117 -1.1811621827371326 2216 118-119 -1.517064745322827 2216 120-121 -1.5102522561142244 2216 122-123 -1.4810547766514794 2216 124-125 -1.7729421247699797 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22.0 3 15.0 4 49.0 5 110.0 6 367.0 7 592.0 8 834.0 9 1249.0 10 2070.0 11 2659.0 12 2939.0 13 3823.0 14 5378.0 15 4088.0 16 1758.0 17 1527.0 18 1590.0 19 1606.0 20 1857.0 21 2036.0 22 2198.0 23 2453.0 24 2749.0 25 3143.0 26 3757.0 27 4488.0 28 5276.0 29 6691.0 30 8077.0 31 10022.0 32 12217.0 33 15214.0 34 20107.0 35 28520.0 36 44599.0 37 91768.0 38 47450.0 39 86.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.85850582383094 20.803767349829087 12.991062452099463 21.34666437424051 2 17.561097779745126 24.649663350651167 38.41501060037742 19.37422826922629 3 14.289832956689885 32.705076532395225 34.49345339328565 18.511637117629242 4 9.280863406565244 19.403058966055493 42.54974023250938 28.766337394869883 5 7.388671635963097 43.772255473899534 38.1730550682744 10.666017821862962 6 23.49563024378027 45.743965193384646 17.95808179205144 12.802322770783645 7 21.70077813759523 36.97260210143746 27.247338256878596 14.079281504088717 8 24.90680986883489 34.30124874775761 22.70927008829765 18.082671295109847 9 26.021596812897513 11.909407543741118 22.641998462362835 39.42699718099853 10-11 20.39072874682571 28.375230063136314 32.65455000815414 18.57949118188384 12-13 22.989131700952868 28.922285254991497 32.0625014560958 16.026081587959833 14-15 21.096643990401418 29.94766791696759 30.077114833539127 18.878573259091862 16-17 16.98928895929921 30.47229923351117 32.76026256319456 19.77814924399506 18-19 16.25483423805419 27.836329007758074 37.67211052349556 18.23672623069217 20-21 16.506447592199986 28.012953428231953 39.03326887682594 16.447330102742118 22-23 16.756313631386437 27.413333178016448 38.82810497868276 17.00224821191436 24-25 16.920270018405052 28.239085105887284 38.420689373995295 16.41995550171237 26-27 15.462572513570812 28.364891782960182 38.91328658295087 17.259249120518135 28-29 16.55566363022156 25.514293036367448 40.52139878386879 17.408644549542203 30-31 16.956963632551314 27.29699112364001 39.045936910281206 16.70010833352748 32-33 16.303322228176036 28.25699508422058 39.17523821727279 16.26444447033059 34-35 16.368287934627027 27.542383370196877 39.12273012535547 16.966598569820622 36-37 16.90352491671132 27.314755492393356 37.36341821401113 18.418301376884187 38-39 15.971041166740443 28.53277962863733 38.96264823055238 16.533530974069848 40-41 15.59449479300142 27.686351140414228 40.184312606294995 16.534841460289353 42-43 16.680839662643866 27.468139881645776 37.352785284935464 18.498235170774894 44-45 16.209462509647448 27.959837485983897 37.233184313611275 18.59751569075738 46-47 17.11014545094453 26.67338486541273 37.41459052966978 18.801879153972962 48-49 16.86046426964394 28.644426419770703 35.86767688976326 18.62743242082209 50-51 17.404378507626127 27.539106299820304 35.431574532777645 19.62494065977592 52-53 16.810512413157408 27.85690466864027 35.28586884820985 20.046714069992472 54-55 18.117693656331728 28.154651305021467 35.213445710990605 18.514209327656204 56-57 18.15894317466974 28.337235851913984 34.036077910579905 19.467743062836373 58-59 16.55121799921659 27.46828862700785 35.77913653036588 20.20135684340967 60-61 18.089738101270612 28.804346243341865 34.50231087114163 18.603604784245885 62-63 17.52395679985788 29.006620973648612 35.768110117552496 17.701312108941007 64-65 17.921451224448788 27.446349369064503 35.899691011972195 18.732508394514515 66-67 18.259495744479473 28.35494463090913 34.71529147949414 18.670268145117255 68-69 18.07337686165101 28.739319350686415 35.24235681959823 17.94494696806435 70-71 18.89107146237439 26.864389380170163 36.08677355614027 18.157765601315173 72-73 17.765999271933016 28.465671641791047 34.58856934838005 19.179759737895886 74-75 17.933843261794827 28.263607678340268 34.861225779490155 18.941323280374746 76-77 16.88908691935937 27.9745705578198 37.14105157092039 17.995290951900436 78-79 16.625313425533587 27.687887868648126 35.95140120506839 19.73539750074989 80-81 16.940025976900362 29.329434856806706 34.194279257503304 19.536259908789628 82-83 18.76971195429011 27.315126258434457 34.11234685467831 19.80281493259713 84-85 16.509990302373733 27.777729241109782 34.54772076660755 21.164559689908934 86-87 16.890348226831595 29.14854426186541 35.05420340291075 18.906904108392244 88-89 17.712592463160348 27.91178868891607 34.98616692876696 19.38945191915662 90-91 17.81377638313518 28.45993112536494 34.54710923109406 19.17918326040582 92-93 17.753260217483735 28.819943969107293 33.61227437664176 19.814521436767215 94-95 17.4065175607199 27.263964121381562 34.52050206768012 20.809016250218416 96-97 18.326754140086578 28.03726478352731 34.09177282926666 19.54420824711945 98-99 17.562012653712024 28.36819535351035 34.16895399378235 19.900837998995275 100-101 17.407391228376724 27.8978100064069 34.24995631661716 20.44484244859922 102-103 18.542959569690808 27.05027360279107 33.47477000765913 20.93199681985899 104-105 17.088452768207908 29.133382452727435 33.34590440356339 20.432260375501265 106-107 17.56137124451958 27.431151649113307 34.07128805716091 20.936189049206202 108-109 18.77142299661309 27.50501629385435 32.40802490528014 21.31553580425242 110-111 17.87332128644463 28.446868706871825 31.90149120290756 21.77831880377599 112-113 17.225380663350304 27.38766499295176 34.381589524331005 21.00536481936694 114-115 17.909897785895094 28.01653951802947 32.671373933509855 21.402188762565576 116-117 17.782350910767107 28.209239767614935 32.485556036171445 21.522853285446512 118-119 17.165995086567573 26.116626303901768 33.589150255792646 23.12822835373801 120-121 17.09177432695332 27.421919595531275 33.14019524469659 22.346110832818816 122-123 17.3439654707939 27.79621477655474 32.94516129501997 21.91465845763139 124-125 17.391013114248018 27.25917167345726 32.39463897229495 22.955176239999766 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 273.0 1 202.0 2 397.0 3 751.0 4 586.0 5 507.5 6 894.0 7 2350.0 8 3628.0 9 3726.0 10 2783.5 11 1527.5 12 1187.0 13 1012.0 14 924.5 15 868.0 16 775.0 17 743.5 18 733.5 19 725.0 20 758.5 21 787.0 22 823.0 23 995.5 24 1103.0 25 1172.0 26 1529.0 27 2015.5 28 2315.0 29 3237.5 30 5452.0 31 6964.5 32 9068.5 33 12739.0 34 15738.0 35 18538.5 36 23885.5 37 27766.0 38 25934.5 39 24079.0 40 21702.0 41 17637.5 42 13297.5 43 11731.0 44 12636.5 45 12884.5 46 9996.0 47 6511.5 48 5721.0 49 4578.0 50 4576.0 51 3460.5 52 1619.0 53 1155.5 54 840.5 55 731.0 56 677.5 57 584.5 58 504.0 59 488.0 60 444.5 61 375.0 62 339.5 63 295.5 64 231.0 65 162.5 66 107.0 67 103.0 68 97.5 69 78.0 70 79.5 71 83.0 72 82.5 73 65.5 74 61.0 75 47.5 76 22.0 77 10.0 78 2.5 79 0.5 80 3.0 81 2.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0655243109754677 2 0.0 3 0.0 4 0.0 5 0.06115602357710319 6 2.9121915989096755E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 1.4560957994548377E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0023297532791277404 44-45 0.00844535563683806 46-47 0.011066328075856767 48-49 0.008008526897001607 50-51 0.0072804789972741885 52-53 0.005387554457982899 54-55 0.0052419448780374155 56-57 0.004659506558255481 58-59 0.003931458658528062 60-61 0.002038534119236773 62-63 0.0016017053794003214 64-65 0.0014560957994548377 66-67 0.001892924539291289 68-69 0.0017473149593458053 70-71 0.0021841436991822567 72-73 0.0026209724390187077 74-75 7.280478997274189E-4 76-77 7.280478997274189E-4 78-79 2.9121915989096755E-4 80-81 5.824383197819351E-4 82-83 2.9121915989096755E-4 84-85 2.9121915989096755E-4 86-87 4.368287398364513E-4 88-89 0.0011648766395638702 90-91 0.001892924539291289 92-93 5.824383197819351E-4 94-95 0.0011648766395638702 96-97 0.0010192670596183865 98-99 0.001892924539291289 100-101 0.0011648766395638702 102-103 8.736574796729026E-4 104-105 8.736574796729026E-4 106-107 7.280478997274189E-4 108-109 8.736574796729026E-4 110-111 0.0010192670596183865 112-113 0.010483889756074831 114-115 0.02475362859073224 116-117 0.021695827411877082 118-119 0.011503156815693218 120-121 0.005824383197819351 122-123 0.00655243109754677 124-125 0.004950725718146448 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 343384.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.061854949560841 #Duplication Level Percentage of deduplicated Percentage of total 1 60.6631863882444 9.137001141579107 2 10.616782675947409 3.1981688139226057 3 6.463650425367362 2.9206369545465134 4 4.327146171693736 2.6069939193439415 5 2.919566898685228 2.1987046571768047 6 2.082366589327146 1.8818582112154325 7 1.4907192575406034 1.571709805931552 8 1.1813611755607114 1.4234792535470493 9 0.9551430781129158 1.2947603848752418 >10 7.070765661252901 22.245940404911117 >50 1.24323279195669 13.065256389348367 >100 0.9106728538283062 25.339270321272977 >500 0.056071152358855375 5.914661137385551 >1k 0.015467904098994586 4.065128252918016 >5k 0.0038669760247486465 3.13643035202572 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5430 1.5813200382079537 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5340 1.5551103138177667 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4843 1.410374391351956 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1406 0.4094541388067004 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1364 0.3972229340912797 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1360 0.39605805745171585 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1350 0.39314586585280614 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 1321 0.38470051021596813 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1263 0.36780979894229204 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1052 0.3063625562052979 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 988 0.28772452997227593 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 955 0.278114297695874 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 934 0.2719986953381637 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 894 0.26034992894252496 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 892 0.25976749062274307 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 850 0.2475362859073224 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 812 0.23646995783146565 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 804 0.23414020455233792 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 769 0.22394753395615402 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 748 0.21783193159844372 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 736 0.21433730167975212 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 727 0.21171632924073341 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 712 0.20734804184236888 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 690 0.2009412203247676 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 689 0.2006500011648766 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 688 0.2003587820049857 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 683 0.19890268620553084 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 647 0.188418796449456 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 626 0.1823031940917457 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 615 0.17909978333294505 No Hit CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC 581 0.16919833189665215 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 572 0.16657735945763344 No Hit CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC 544 0.15842322298068634 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 535 0.15580225054166763 No Hit GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG 535 0.15580225054166763 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 526 0.15318127810264895 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 526 0.15318127810264895 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 523 0.152307620622976 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 509 0.14823055238450247 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 485 0.14124129254711926 No Hit GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 475 0.1383291009482096 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 446 0.12988374531137153 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 444 0.1293013069915896 No Hit GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA 441 0.12842764951191668 No Hit GAGTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCA 434 0.1263891153926799 No Hit GGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATG 432 0.12580667707289797 No Hit GGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTC 428 0.12464180043333412 No Hit GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA 426 0.12405936211355219 No Hit GGTTAATAGTGTAATTGAATTATAGTGAAATCATATTACTAGACCTGATG 425 0.1237681429536612 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 425 0.1237681429536612 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 420 0.12231204715420638 No Hit TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA 418 0.12172960883442444 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 410 0.11939985555529668 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 407 0.11852619807562378 No Hit CCTTCTAACATCAGGTCTAGTAATATGATTTCACTATAATTCAATTACAC 405 0.11794375975584186 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 404 0.1176525405959509 No Hit GTACATGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACAT 401 0.11677888311627797 No Hit ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT 400 0.11648766395638702 No Hit TTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAATGGTAGCTGTTG 400 0.11648766395638702 No Hit CAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGT 397 0.11561400647671413 No Hit AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT 374 0.10891596579922186 No Hit GCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGT 369 0.10745986999976702 No Hit GTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGA 366 0.10658621252009413 No Hit GGTCTAGTAATATGATTTCACTATAATTCAATTACACTATTAACCCTTGG 363 0.10571255504042121 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 361 0.10513011672063928 No Hit GGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATAC 360 0.10483889756074832 No Hit CCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGA 360 0.10483889756074832 No Hit GCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTG 359 0.10454767840085734 No Hit GAATAGAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGT 359 0.10454767840085734 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 358 0.10425645924096638 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 353 0.10280036344151156 No Hit ACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCAT 346 0.10076182932227477 No Hit GGCTAATATTTATTAATACTAGAGTAGCTCCTCCGATTAGGTGTATTAAT 346 0.10076182932227477 No Hit ATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATT 344 0.10017939100249285 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.4560957994548377E-4 0.0 20-21 0.0 0.0 0.0 5.824383197819351E-4 0.0 22-23 0.0 0.0 0.0 8.736574796729026E-4 0.0 24-25 0.0 0.0 0.0 0.0014560957994548377 0.0 26-27 0.0 0.0 0.0 0.0014560957994548377 0.0 28-29 0.0 0.0 0.0 0.0023297532791277404 0.0 30-31 0.0 0.0 0.0 0.00844535563683806 0.0 32-33 0.0 0.0 0.0 0.021258998672040633 0.0 34-35 0.0 0.0 0.0 0.03305337464762482 0.0 36-37 0.0 0.0 0.0 0.04950725718146448 0.0 38-39 0.0 0.0 0.0 0.08110453602963447 0.0 40-41 0.0 0.0 0.0 0.11430352025720476 0.0 42-43 0.0 0.0 0.0 0.15099713440346668 0.0 44-45 0.0 0.0 0.0 0.19031172098874727 0.0 46-47 0.0 0.0 0.0 0.242294341029285 0.0 48-49 0.0 0.0 0.0 0.295878666449223 0.0 50-51 0.0 0.0 0.0 0.3475700673298698 0.0 52-53 0.0 0.0 0.0 0.40086317358991685 0.0 54-55 0.0 0.0 0.0 0.46595065582554807 0.0 56-57 0.0 0.0 0.0 0.5348239871397619 0.0 58-59 0.0 0.0 0.0 0.6277228991449806 0.0 60-61 0.0 0.0 0.0 0.7217866877897631 0.0 62-63 0.0 0.0 0.0 0.8219660787922559 0.0 64-65 0.0 0.0 0.0 0.9368520373692426 0.0 66-67 0.0 0.0 0.0 1.069939193439415 0.0 68-69 0.0 0.0 0.0 1.2183153554038628 0.0 70-71 0.0 0.0 0.0 1.394502947137898 0.0 72-73 0.0 0.0 0.0 1.5689432239125876 0.0 74-75 0.0 0.0 0.0 1.7640600610395358 0.0 76-77 0.0 0.0 0.0 1.9629627472450668 0.0 78-79 0.0 0.0 0.0 2.2129743960114623 0.0 80-81 0.0 0.0 0.0 2.4724506674743143 0.0 82-83 0.0 0.0 0.0 2.7370232742352583 0.0 84-85 0.0 0.0 0.0 3.038143885562519 0.0 86-87 0.0 0.0 0.0 3.343632784288144 0.0 88-89 0.0 0.0 0.0 3.677224331943247 0.0 90-91 0.0 0.0 0.0 4.026250495072572 0.0 92-93 0.0 0.0 0.0 4.432501223120472 0.0 94-95 0.0 0.0 0.0 4.871805325815997 0.0 96-97 0.0 0.0 0.0 5.356830836614403 0.0 98-99 0.0 0.0 0.0 5.865299489784032 0.0 100-101 0.0 0.0 0.0 6.4100249283600865 0.0 102-103 0.0 0.0 0.0 6.950382079537777 0.0 104-105 0.0 0.0 0.0 7.478944854739883 0.0 106-107 0.0 0.0 0.0 8.068809263099038 0.0 108-109 0.0 0.0 0.0 8.65648952775901 0.0 110-111 0.0 0.0 0.0 9.272854879668245 0.0 112-113 0.0 0.0 0.0 9.910916058989354 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTAC 25 1.7170072E-5 95.27777 5 TACCTGG 45 4.0199666E-10 92.631165 2 CAGCATA 20 8.4784295E-4 89.244865 9 TAACGCA 35 8.9015884E-7 85.069435 4 TATAACG 35 8.9015884E-7 85.069435 2 CCGAGTA 30 4.2427273E-5 79.39814 4 TGGGTAT 170 0.0 69.99597 6 GGTATCA 2100 0.0 65.002655 1 CATGGGT 295 0.0 60.557903 4 ACCTGGG 70 1.3293175E-8 59.548607 3 CAGCACA 40 1.7630891E-4 59.548603 4 CTGTCAC 15 0.0042343484 59.505245 112-113 GTACAGC 30 0.0042349896 59.49658 6 AAGCGTA 15 0.0042367913 59.49658 22-23 TACGCAC 15 0.0042367913 59.49658 86-87 ACGTGCT 15 0.0042367913 59.49658 64-65 ACTAAGC 15 0.0042367913 59.49658 18-19 TAAGCGA 15 0.0042367913 59.49658 12-13 GTACGCA 15 0.0042367913 59.49658 84-85 ACGCACA 20 1.7719818E-4 59.496574 86-87 >>END_MODULE