FastQCFastQC Report
Thu 26 May 2016
SRR936187_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936187_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973758
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33000.057.6913221
GTACATG170450.056.4051551
TACATGG178650.052.4174842
ACATGGG176900.051.9607123
CATGGGA99450.045.465634
CATGGGG76600.043.183764
GTATCAA44800.042.2302362
ATGGGAG44000.039.6177145
TCAACGC50400.037.183865
CAACGCA51750.036.2138446
ATCAACG51900.036.109184
AACGCAG53150.035.2599537
ATGGGGG44450.033.3274465
ATGGGAT46750.032.9604075
GAGTACA99750.031.4916551
TATCAAC63100.030.454233
AGTACAT99350.030.1812422
ACGCAGA64700.028.2303248
ATGGGGA42950.027.980965
TACCTGG25050.027.78772
Produced by FastQC (version 0.11.5)