FastQCFastQC Report
Thu 26 May 2016
SRR936187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936187_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973758
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73530.24726289092791004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54350.18276537633526332No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53060.1784274308803877No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.077.589821
GTATCAA84300.051.5507242
GTACATG150800.047.3214531
TACATGG156350.045.04292
ACATGGG153500.044.9872133
TCAACGC117500.038.0995185
ATCAACG117750.038.069184
CAACGCA120800.037.2825366
AACGCAG123300.036.6713647
CATGGGA91600.036.5891574
CATGGGG69750.036.0170144
TATCAAC126850.035.901323
ACGCAGA144400.031.2716718
CGCAGAG147750.030.24059
ATGGGGG40400.028.291725
GAGTACA100350.028.1954351
ATGGGAT41400.028.183525
AGTACAT96500.026.649922
CAGAGTA165500.026.5658049
GCAGAGT168550.025.7674038
Produced by FastQC (version 0.11.5)