##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936187_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2973758 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.371566886074792 31.0 31.0 34.0 30.0 34.0 2 31.37009097579561 31.0 31.0 34.0 30.0 34.0 3 31.712967901221283 31.0 31.0 34.0 30.0 34.0 4 35.54878978047306 37.0 35.0 37.0 33.0 37.0 5 35.19130944750716 37.0 35.0 37.0 33.0 37.0 6 35.378586287115496 37.0 35.0 37.0 33.0 37.0 7 35.19935213289044 37.0 35.0 37.0 32.0 37.0 8 35.2849135672775 37.0 35.0 37.0 32.0 37.0 9 36.96322229313885 39.0 37.0 39.0 33.0 39.0 10-11 36.83566921047375 39.0 37.0 39.0 32.0 39.0 12-13 36.787856980964825 39.0 37.0 39.0 32.0 39.0 14-15 37.9379132397458 40.0 37.0 41.0 32.5 41.0 16-17 37.83925474097085 40.0 37.0 41.0 32.0 41.0 18-19 37.91805167064704 40.0 37.0 41.0 32.0 41.0 20-21 37.96784119622377 40.0 37.0 41.0 32.0 41.0 22-23 37.89052353957518 40.0 37.0 41.0 32.0 41.0 24-25 37.866458871232965 40.0 37.0 41.0 32.0 41.0 26-27 37.700691347446565 40.0 37.0 41.0 32.0 41.0 28-29 37.55864633235119 40.0 37.0 41.0 32.0 41.0 30-31 37.416161469763175 39.5 36.0 41.0 31.5 41.0 32-33 37.25309524177825 39.0 36.0 41.0 31.0 41.0 34-35 37.086104013843766 39.0 36.0 41.0 31.0 41.0 36-37 36.90464203879401 39.0 36.0 41.0 30.0 41.0 38-39 36.909938703821894 39.0 35.5 41.0 30.5 41.0 40-41 36.96769609362968 39.5 36.0 41.0 31.0 41.0 42-43 36.881362235931775 40.0 36.0 41.0 31.0 41.0 44-45 36.758226795858974 40.0 36.0 41.0 30.0 41.0 46-47 36.54089354278324 39.5 35.5 41.0 30.0 41.0 48-49 36.31309625732827 39.0 35.0 41.0 30.0 41.0 50-51 36.131863285445554 39.0 35.0 41.0 29.0 41.0 52-53 35.9805201028463 39.0 35.0 41.0 28.5 41.0 54-55 35.7952378774601 39.0 35.0 41.0 28.0 41.0 56-57 35.598609234510675 39.0 35.0 40.5 28.0 41.0 58-59 35.38836330999362 38.0 34.5 40.0 28.0 41.0 60-61 35.16051659213696 38.0 34.0 40.0 27.0 41.0 62-63 34.93282842786804 37.5 34.0 40.0 27.0 41.0 64-65 34.67851906577469 37.0 34.0 40.0 26.5 41.0 66-67 34.39439187721395 37.0 34.0 40.0 26.0 41.0 68-69 34.076806855164406 36.0 33.5 39.0 26.0 41.0 70-71 33.74458261230403 36.0 33.0 39.0 26.0 41.0 72-73 33.478215611357754 35.0 33.0 39.0 26.0 40.0 74-75 33.30213874161919 35.0 33.0 37.5 26.0 40.0 76-77 33.00207279812278 35.0 33.0 37.0 26.0 39.0 78-79 32.67262198201737 35.0 33.0 36.5 26.0 39.0 80-81 32.39878211340667 35.0 33.0 36.0 26.0 38.5 82-83 32.14631957274264 35.0 32.0 36.0 25.0 37.0 84-85 31.898757060930983 35.0 32.0 35.5 25.0 37.0 86-87 31.705251738709066 35.0 32.0 35.0 25.0 36.5 88-89 31.55699202826861 35.0 32.0 35.0 25.0 36.0 90-91 31.427488887797864 35.0 32.0 35.0 25.0 36.0 92-93 31.2998882222427 35.0 32.0 35.0 24.0 36.0 94-95 31.18952786339709 35.0 32.0 35.0 24.0 35.5 96-97 31.12748465073486 35.0 32.0 35.0 24.0 35.0 98-99 31.04527873485334 34.0 32.0 35.0 24.0 35.0 100-101 30.966854397701493 34.0 32.0 35.0 23.0 35.0 102-103 29.93805481145406 33.5 30.0 34.5 19.5 35.0 104-105 30.716708622557718 34.0 31.5 35.0 23.0 35.0 106-107 30.872696433267265 34.0 32.0 35.0 23.0 35.0 108-109 30.847539880514823 34.0 32.0 35.0 23.0 35.0 110-111 30.81122572852263 34.0 32.0 35.0 22.5 35.0 112-113 30.734730431998837 34.0 32.0 35.0 20.0 35.0 114-115 30.72077889323879 34.0 32.0 35.0 20.0 35.0 116-117 30.6244874330729 34.0 32.0 35.0 19.0 35.0 118-119 30.491785982585 34.0 32.0 35.0 18.5 35.0 120-121 30.294680165635533 34.0 32.0 35.0 16.5 35.0 122-123 30.066522393550517 34.0 32.0 35.0 2.0 35.0 124-125 27.94443747608245 32.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23407730873962151 1101 2 0.6668596580019575 1101 3 0.4565823779929197 1101 4 0.32049114495202247 1101 5 0.5988180946035442 1101 6 0.3803985382082473 1101 7 0.5331869720723432 1101 8 0.5155377961202277 1101 9 0.33397914349383484 1101 10-11 0.3945313055616282 1101 12-13 0.48550109521345064 1101 14-15 0.5579205818820938 1101 16-17 0.6303366786436655 1101 18-19 0.5993191733787882 1101 20-21 0.47745378781274894 1101 22-23 0.5032567420738232 1101 24-25 0.44551846898199443 1101 26-27 0.46406913532175054 1101 28-29 0.5511102588610441 1101 30-31 0.47273298867308 1101 32-33 0.3454129871751448 1101 34-35 0.43269567636928485 1101 36-37 0.41821579541685594 1101 38-39 0.4418336249144019 1101 40-41 0.402720973306252 1101 42-43 0.4011901174743926 1101 44-45 0.36872073460198607 1101 46-47 0.3736543512005355 1101 48-49 0.4222279183119326 1101 50-51 0.34607652968387015 1101 52-53 0.3716907329580792 1101 54-55 0.34477916241679907 1101 56-57 0.21895320918233807 1101 58-59 0.38570060120959937 1101 60-61 0.4514678396386813 1101 62-63 0.3176643600261855 1101 64-65 0.24233146320075605 1101 66-67 0.310135465597817 1101 68-69 0.4408032447801844 1101 70-71 0.3564765528573517 1101 72-73 0.35473869228383137 1101 74-75 0.3716716019400934 1101 76-77 0.4056693312511115 1101 78-79 0.45167289084206175 1101 80-81 0.39992078940526454 1101 82-83 0.40382058398443377 1101 84-85 0.377887985154274 1101 86-87 0.3265181707254037 1101 88-89 0.3222373530048479 1101 90-91 0.3803543363662989 1101 92-93 0.3631253556876253 1101 94-95 0.33940188287042616 1101 96-97 0.33053422372041297 1101 98-99 0.3250239500748435 1101 100-101 0.3893255123574484 1101 102-103 0.4059397307167707 1101 104-105 0.24094206274921248 1101 106-107 0.3172502834380566 1101 108-109 0.24827529935929604 1101 110-111 0.3219061020299421 1101 112-113 0.41028331935824625 1101 114-115 0.30079806551378496 1101 116-117 0.3411529274947185 1101 118-119 0.48055639582566556 1101 120-121 0.5103447974048869 1101 122-123 0.45691162978077315 1101 124-125 0.4521610766762194 1102 1 0.2180483795189616 1102 2 0.6304277468124795 1102 3 0.38628123565127126 1102 4 0.2172385578449294 1102 5 0.48981738072143344 1102 6 0.26155791306970855 1102 7 0.4412921494132078 1102 8 0.3911085751249743 1102 9 0.36333070064548423 1102 10-11 0.39639007170663376 1102 12-13 0.36717573796545366 1102 14-15 0.43895029099220295 1102 16-17 0.534063164562049 1102 18-19 0.418018245916322 1102 20-21 0.3741274161755115 1102 22-23 0.424027750540958 1102 24-25 0.37258561918144295 1102 26-27 0.3672545741638231 1102 28-29 0.34981478963781854 1102 30-31 0.2822417222703777 1102 32-33 0.30414495205875625 1102 34-35 0.32994688877069933 1102 36-37 0.36588199546578437 1102 38-39 0.36114232584753836 1102 40-41 0.20160984697414364 1102 42-43 0.27551595524956696 1102 44-45 0.32243169971351193 1102 46-47 0.29242427504691904 1102 48-49 0.24007232991149152 1102 50-51 0.2444721326469832 1102 52-53 0.30140098004229543 1102 54-55 0.29981593960327046 1102 56-57 0.2754139883225051 1102 58-59 0.3050998056394576 1102 60-61 0.34526876660767414 1102 62-63 0.2990274101941708 1102 64-65 0.24731093203347143 1102 66-67 0.1730914191547015 1102 68-69 0.28195470507630915 1102 70-71 0.2689047575289294 1102 72-73 0.3548966243399221 1102 74-75 0.35908662192014873 1102 76-77 0.3395734333906901 1102 78-79 0.23395856740928878 1102 80-81 0.25488337938518413 1102 82-83 0.22769114673221225 1102 84-85 0.26108490264268625 1102 86-87 0.2305095150775749 1102 88-89 0.21834320763164072 1102 90-91 0.2464292555753964 1102 92-93 0.240174588760798 1102 94-95 0.12438760345176547 1102 96-97 0.1238652154772737 1102 98-99 0.1919841059943046 1102 100-101 0.2116649178009915 1102 102-103 0.21786588475873714 1102 104-105 0.2335741898601036 1102 106-107 0.18014587501174617 1102 108-109 0.16538179459687896 1102 110-111 0.22880601361088893 1102 112-113 0.1663092829855941 1102 114-115 0.15488637395033678 1102 116-117 0.20437660164613192 1102 118-119 0.13229138042704847 1102 120-121 0.16835740851939818 1102 122-123 0.12561841830889975 1102 124-125 0.0882010771294297 1103 1 0.15860573522422783 1103 2 0.5668825373516029 1103 3 0.33778093217264527 1103 4 0.20950286415596509 1103 5 0.3853081406368588 1103 6 0.20697439670128404 1103 7 0.2949496032893606 1103 8 0.31294924856754136 1103 9 0.27315164227396593 1103 10-11 0.37672805492226047 1103 12-13 0.3940619140574029 1103 14-15 0.3503930624376608 1103 16-17 0.4935254358552683 1103 18-19 0.5085159189543802 1103 20-21 0.37303750154916315 1103 22-23 0.385520724187181 1103 24-25 0.2887448728306268 1103 26-27 0.27024792475531 1103 28-29 0.262027843996826 1103 30-31 0.17905161111879409 1103 32-33 0.1817289375447544 1103 34-35 0.23767891710473776 1103 36-37 0.15864898985002185 1103 38-39 0.11701704425535553 1103 40-41 0.21173320630902737 1103 42-43 0.10980601668155288 1103 44-45 0.13211350941450206 1103 46-47 0.1972698264028523 1103 48-49 0.26174528644421 1103 50-51 0.20654607900900857 1103 52-53 0.20578764411702366 1103 54-55 0.20576069859641422 1103 56-57 0.2277095534504383 1103 58-59 0.2902698553742056 1103 60-61 0.24781752839174942 1103 62-63 0.13289605551967298 1103 64-65 0.18495474935345158 1103 66-67 0.1388156242891796 1103 68-69 0.1639407259949195 1103 70-71 0.18445607676554943 1103 72-73 0.19321845654197034 1103 74-75 0.23726657656865768 1103 76-77 0.3249815317750375 1103 78-79 0.24631264819370813 1103 80-81 0.22672495819832506 1103 82-83 0.20601743240032988 1103 84-85 0.253738179290508 1103 86-87 0.19866001367352837 1103 88-89 0.24054710219289888 1103 90-91 0.25442660934069394 1103 92-93 0.17328008645018755 1103 94-95 0.1710699267810618 1103 96-97 0.257106530719895 1103 98-99 0.24008297533401546 1103 100-101 0.18651318447099996 1103 102-103 0.16806055140009235 1103 104-105 0.1642151651141326 1103 106-107 0.10140882629810477 1103 108-109 0.09437341995883841 1103 110-111 0.14455033233068804 1103 112-113 0.03325003896445011 1103 114-115 0.16271166742003018 1103 116-117 0.24786964740292206 1103 118-119 0.19980426680191954 1103 120-121 0.23659925781101165 1103 122-123 0.22433228953552842 1103 124-125 0.31717440363565785 1104 1 0.22044582946605829 1104 2 0.4082374886637403 1104 3 0.29070156107293954 1104 4 0.232756119790146 1104 5 0.4320709883986922 1104 6 0.1815906553456088 1104 7 0.38523364662388815 1104 8 0.304076871998781 1104 9 0.3257899796063626 1104 10-11 0.34357221519123016 1104 12-13 0.27925904405294943 1104 14-15 0.32140638711394587 1104 16-17 0.48185183979980195 1104 18-19 0.3868991588869406 1104 20-21 0.38707473703641426 1104 22-23 0.37615568334208405 1104 24-25 0.3051236497760854 1104 26-27 0.22280916024899255 1104 28-29 0.39463215848554256 1104 30-31 0.28875242062783 1104 32-33 0.26075944721241484 1104 34-35 0.21645602328268154 1104 36-37 0.3872680352497042 1104 38-39 0.32645922711729014 1104 40-41 0.33740221241449575 1104 42-43 0.3983270836842152 1104 44-45 0.4032496184676049 1104 46-47 0.1437901187710935 1104 48-49 0.195346822213196 1104 50-51 0.19356388113651235 1104 52-53 0.20303407589695865 1104 54-55 0.22419205586198387 1104 56-57 0.20183519690324658 1104 58-59 0.33156265111340133 1104 60-61 0.2517810209322704 1104 62-63 0.22862793236887313 1104 64-65 0.1544973534429488 1104 66-67 0.17067933523804157 1104 68-69 0.20812036082389795 1104 70-71 0.15637754779952218 1104 72-73 0.18041633706505422 1104 74-75 0.21295619405675126 1104 76-77 0.20867515582663998 1104 78-79 0.2128768472265392 1104 80-81 0.18854962403678854 1104 82-83 0.1301380134082848 1104 84-85 0.16316601855869095 1104 86-87 0.11491801514887001 1104 88-89 0.17522822441639008 1104 90-91 0.2799025996915141 1104 92-93 0.27771125246784223 1104 94-95 0.2149389042796983 1104 96-97 0.22170131816952932 1104 98-99 0.20710351761025336 1104 100-101 0.22851572694330358 1104 102-103 0.2161614113728625 1104 104-105 0.25461299292350503 1104 106-107 0.33400116770295796 1104 108-109 0.24051418967545146 1104 110-111 0.26078442781643574 1104 112-113 0.300235827154971 1104 114-115 0.3230149078668738 1104 116-117 0.3245486916133302 1104 118-119 0.2731923602530486 1104 120-121 0.3500703432152612 1104 122-123 0.369146658521867 1104 124-125 0.4346581596161734 1105 1 0.20961703957191347 1105 2 0.621209476557766 1105 3 0.25052173124779387 1105 4 0.18110129856569301 1105 5 0.38642801314491493 1105 6 0.30471455449632145 1105 7 0.3238873882035307 1105 8 0.29962282190389544 1105 9 0.26059956735960554 1105 10-11 0.13506509762092378 1105 12-13 0.20110365922536744 1105 14-15 0.19347711732481798 1105 16-17 0.3713706448026315 1105 18-19 0.33056353837654484 1105 20-21 0.208001337043072 1105 22-23 0.29071099175427406 1105 24-25 0.28682921906280967 1105 26-27 0.21466374747113548 1105 28-29 0.2795073017643972 1105 30-31 0.21879163503958665 1105 32-33 0.17748922527486144 1105 34-35 0.05266471022215313 1105 36-37 0.1138605906151895 1105 38-39 0.0960941771698387 1105 40-41 0.04278711926540524 1105 42-43 0.01759696000861055 1105 44-45 0.07817149879348761 1105 46-47 0.09404263041175653 1105 48-49 -0.09026227343762372 1105 50-51 -0.06715520035685074 1105 52-53 -0.0542246726345752 1105 54-55 -0.08569878269386066 1105 56-57 -0.15715197418953863 1105 58-59 -0.04979179580247717 1105 60-61 -0.18003512871505478 1105 62-63 -0.17662657685249883 1105 64-65 -0.09926067453270093 1105 66-67 -0.0032745507698592746 1105 68-69 -0.09741904145556646 1105 70-71 -0.14044760681480994 1105 72-73 -0.08687708376019998 1105 74-75 -0.03604957356120053 1105 76-77 -0.027242851412218272 1105 78-79 0.014856077143321045 1105 80-81 -0.05546421317306738 1105 82-83 0.0029854616574667148 1105 84-85 0.05646171757718221 1105 86-87 0.06998021759257966 1105 88-89 0.028333669726276156 1105 90-91 -0.02096880525772704 1105 92-93 0.001058294066670129 1105 94-95 0.11835730390047416 1105 96-97 -0.01705468051777359 1105 98-99 -0.03781347181366712 1105 100-101 -0.009464544569134148 1105 102-103 -0.034708566733016255 1105 104-105 0.010434830919713534 1105 106-107 0.04179212880879035 1105 108-109 0.058763793726416935 1105 110-111 0.054485350349938955 1105 112-113 0.024445078490256122 1105 114-115 0.08174596057605399 1105 116-117 0.11980384291421586 1105 118-119 0.09015110704066842 1105 120-121 0.09087473408790103 1105 122-123 0.07467448751627614 1105 124-125 0.2100708740588395 1106 1 0.1942906349090805 1106 2 0.5754697239138267 1106 3 0.31551975117883657 1106 4 0.17459510667680433 1106 5 0.3356324950817182 1106 6 0.22517464935751974 1106 7 0.28649148160272375 1106 8 0.2465588109631156 1106 9 0.1576709662978928 1106 10-11 0.19755847808825422 1106 12-13 0.16222909080292425 1106 14-15 0.12135136832919358 1106 16-17 0.14087707966730534 1106 18-19 0.12523809539302988 1106 20-21 0.15991932816207566 1106 22-23 0.1309031327255994 1106 24-25 0.21740843276376864 1106 26-27 0.2671580193289316 1106 28-29 0.20747392669971987 1106 30-31 0.2082850888309693 1106 32-33 0.16011757471844845 1106 34-35 0.2226508345634599 1106 36-37 0.24092590477521014 1106 38-39 0.17879296722710336 1106 40-41 0.23894601712340346 1106 42-43 0.23339616104798466 1106 44-45 0.27756448265596845 1106 46-47 0.26503236775988626 1106 48-49 0.22873066940172038 1106 50-51 0.15349349227846432 1106 52-53 0.17116380507339812 1106 54-55 0.2640499266669991 1106 56-57 0.2584805144687934 1106 58-59 0.2845976808059518 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2216 72-73 -1.1298483461010846 2216 74-75 -1.189616856549236 2216 76-77 -1.0208916253714833 2216 78-79 -0.9587125767716103 2216 80-81 -0.9178959976832672 2216 82-83 -0.9854946803759894 2216 84-85 -0.9316171568692155 2216 86-87 -0.8693168475606079 2216 88-89 -0.9457386188416201 2216 90-91 -0.8237604060456256 2216 92-93 -0.9020514477262651 2216 94-95 -1.0270772267617971 2216 96-97 -1.1561909784767437 2216 98-99 -1.1443230105012923 2216 100-101 -1.2963352953461644 2216 102-103 -1.2956582797319598 2216 104-105 -1.0723416104934103 2216 106-107 -0.9627474829474281 2216 108-109 -0.9618645274061954 2216 110-111 -0.9382176876557295 2216 112-113 -0.7942448063088996 2216 114-115 -0.9335309949346531 2216 116-117 -0.9728499457870399 2216 118-119 -1.023475318095528 2216 120-121 -1.062089431557169 2216 122-123 -1.2463672947610753 2216 124-125 -1.4336809908881207 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82.0 3 96.0 4 231.0 5 494.0 6 1368.0 7 2699.0 8 4270.0 9 6976.0 10 10654.0 11 13270.0 12 14697.0 13 17877.0 14 23464.0 15 17986.0 16 10210.0 17 9415.0 18 9242.0 19 9041.0 20 9125.0 21 9437.0 22 10840.0 23 13192.0 24 17167.0 25 22766.0 26 29797.0 27 38633.0 28 49334.0 29 62556.0 30 78916.0 31 98377.0 32 124038.0 33 158360.0 34 212084.0 35 301260.0 36 505106.0 37 784440.0 38 295015.0 39 1243.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.6865609114381 18.158434985031114 10.325553505566097 23.829450597964687 2 19.415351970824766 20.815803588392452 36.14154494906271 23.627299491720063 3 18.124532156997518 25.931701142730702 31.87751912229458 24.066247577977194 4 11.952287964349475 16.325644630680987 36.989908724889084 34.73215868008045 5 10.959898763581942 40.76700516444357 35.862853051409246 12.410243020565245 6 32.07537271900341 37.65273198363685 17.190555375274695 13.081339922085041 7 27.126518028703074 33.66383545668478 22.318964757724068 16.890681756888085 8 27.069889345400668 33.56648389008117 19.81291012920352 19.55071663531464 9 26.459718645565644 11.599867911242272 19.136358775663656 42.804054667528426 10-11 24.424633746256422 24.927700909085406 30.19230213083916 20.455363213819012 12-13 25.60265495712832 24.141961114522434 29.54546065954257 20.709923268806673 14-15 22.70630293386348 25.92832032734338 26.593791424857034 24.771585313936104 16-17 22.258291447485455 28.22944174658521 27.0671914349424 22.445075370986935 18-19 21.404986552369092 26.726973075818545 29.688074819807124 22.17996555200524 20-21 22.409742151177063 26.121022625243885 29.75632179888209 21.712913424696968 22-23 22.021765052838866 26.496843388063184 29.543173990620623 21.938217568477327 24-25 22.015678478208383 26.522316207304026 29.550134879838915 21.911870434648684 26-27 21.899041549446864 26.570773411958875 29.540836880472455 21.98934815812181 28-29 21.867119606612107 26.421762100938306 29.679308026849537 22.031810265600047 30-31 21.907835136551125 26.23241366647858 29.807637339689375 22.052113857280922 32-33 21.69984914710612 26.68668734981125 29.637919427202885 21.975544075879746 34-35 22.252597222231564 26.56746321316424 29.285862246843553 21.894077317760647 36-37 22.363151271892335 26.22795802482919 29.098147865428185 22.310742837850288 38-39 22.01342543677058 26.555187073057056 29.3616360174567 22.06975147271567 40-41 22.1629275967955 26.44333858538855 29.357363321442087 22.036370496373863 42-43 22.194232235620312 26.533010837572608 29.045955645903692 22.22680128090339 44-45 22.15944317087103 26.748729897078487 28.874841746849107 22.216985185201377 46-47 22.40625419864927 26.91996926777911 28.164560314505838 22.50921621906578 48-49 22.455354635581298 26.93408315954759 28.055207199354026 22.55535500551709 50-51 22.45779070971873 26.89597096501246 28.110055570577053 22.53618275469175 52-53 22.628405893409212 26.85763087263444 28.04884651691794 22.465116717038406 54-55 22.349496484581444 27.05620415507626 28.050506071291707 22.54379328905059 56-57 22.398950091513882 27.320236380741473 27.93047466994889 22.350338857795744 58-59 22.60696128590962 26.81501093270685 27.92131275881527 22.656715022568257 60-61 22.523316573569634 26.8457248142417 28.337592072070738 22.293366540117923 62-63 22.272697924611293 26.89542819940626 28.417147884055865 22.41472599192658 64-65 22.052849817423837 26.926642164004626 28.371412559578747 22.64909545899279 66-67 22.180493036974664 27.46296959835678 27.932406118160664 22.424131246507894 68-69 22.328527673792657 26.944671246346324 28.11453750122742 22.612263578633602 70-71 22.245218458641244 26.822349449754935 28.224099809158705 22.708332282445124 72-73 22.30951935778064 26.993366486329435 27.886735259381616 22.81037889650831 74-75 22.05141552769382 27.25593631167839 27.963784228034 22.72886393259379 76-77 22.07035364803201 27.20849060616898 27.97478321443398 22.746372531365033 78-79 22.349743396773338 27.09330914841827 27.867582882178066 22.68936457263032 80-81 22.170579599929315 27.25528831530699 27.5938844209978 22.980247663765894 82-83 22.385564237251003 27.13636150407221 27.776209426907776 22.701864831769008 84-85 22.09098487531051 27.03274690146808 27.825433703674186 23.050834519547227 86-87 22.10329258003455 26.985987711799286 27.897010068442274 23.01370963972389 88-89 22.270330746054643 26.982599047353705 27.72685291284492 23.020217293746732 90-91 22.13901141185635 27.173336431600237 27.773379038374852 22.91427311816856 92-93 22.24396626603747 26.87177657487762 27.822162604048305 23.062094555036605 94-95 22.05630390766579 26.81551283090299 27.846931737665866 23.28125152376536 96-97 22.233326955211123 26.91813618490322 27.801545343909133 23.046991515976522 98-99 22.131895488817758 26.981392438840295 27.47210933949354 23.414602732848405 100-101 21.915941874979826 26.70491383206534 27.78717488620345 23.59196940675139 102-103 22.004610716291978 26.52665033361362 27.768409615618395 23.70032933447601 104-105 21.886516451533172 26.54123518067346 27.87208678648232 23.700161581311043 106-107 21.829947838421006 26.61109804302023 27.89848340835389 23.660470710204876 108-109 21.978440848020437 26.60512881905206 27.52091610338614 23.895514229541362 110-111 21.910081660028823 26.70093113651989 27.569637675761914 23.819349527689372 112-113 21.902446792392674 26.390959780872116 27.81714411014778 23.88944931658743 114-115 21.589669576179375 26.66512731906976 27.65612297649507 24.089080128255798 116-117 21.459095601816312 26.678860159550215 27.833839330775014 24.028204907858463 118-119 21.54151629951606 26.64453499871276 27.42589206725664 24.388056634514538 120-121 21.46707616356447 26.6648226414904 27.59409362659499 24.27400756835014 122-123 21.492062029435463 26.581776162951027 27.43923437123767 24.48692743637584 124-125 21.43264539782478 26.563189929455476 27.153151601956292 24.851013070763454 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 18.0 1 30.5 2 216.0 3 442.5 4 412.0 5 400.5 6 647.5 7 1351.5 8 2133.5 9 2608.0 10 2498.0 11 2151.5 12 2226.0 13 2376.0 14 2573.0 15 2894.0 16 3251.0 17 3595.5 18 3954.5 19 4311.0 20 4850.0 21 5746.5 22 6963.0 23 8560.5 24 10741.5 25 13759.0 26 17514.5 27 21548.0 28 25172.0 29 30147.5 30 37137.5 31 43837.0 32 50167.5 33 55549.0 34 62137.5 35 67914.5 36 74342.5 37 80904.0 38 83243.0 39 85257.0 40 87401.5 41 91484.5 42 96143.5 43 100531.0 44 106082.0 45 109775.5 46 110105.0 47 109440.5 48 110956.5 49 109879.0 50 107542.5 51 104651.5 52 100533.5 53 97731.5 54 95064.5 55 89931.5 56 81663.5 57 74705.5 58 69506.5 59 61688.5 60 50629.0 61 40589.5 62 33412.5 63 26714.5 64 20290.0 65 15858.5 66 12030.5 67 9780.0 68 7374.5 69 4770.0 70 3547.0 71 2685.0 72 2208.5 73 1724.5 74 1303.0 75 1096.5 76 667.5 77 356.0 78 181.0 79 75.0 80 31.0 81 14.5 82 8.5 83 6.5 84 3.0 85 2.5 86 2.5 87 1.0 88 1.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06480016194996366 2 1.0088245243896779E-4 3 1.3450993658529038E-4 4 3.3627484146322596E-5 5 0.05733486046948003 6 1.0088245243896779E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.3627484146322596E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.3627484146322596E-5 30-31 0.0 32-33 0.0 34-35 1.3450993658529038E-4 36-37 0.0 38-39 0.0 40-41 5.0441226219483894E-5 42-43 0.0025388750530473563 44-45 0.009365254334750845 46-47 0.011147510994505942 48-49 0.00904579323536078 50-51 0.00813785116341007 52-53 0.0066918693451181975 54-55 0.007213095349386198 56-57 0.00556534862621639 58-59 0.003766278224388131 60-61 0.002303482664023098 62-63 0.0016813742073161298 64-65 0.0022698551798767757 66-67 0.002908777378656905 68-69 0.0014123543341455491 70-71 0.0027910811841447758 72-73 0.002353923890242582 74-75 8.743145878043876E-4 76-77 8.238733615849037E-4 78-79 1.1769619451212909E-4 80-81 2.858336152437421E-4 82-83 1.513236786584517E-4 84-85 3.6990232560954857E-4 86-87 3.6990232560954857E-4 88-89 0.0013955405920723879 90-91 0.0017654429176819366 92-93 6.389221987801294E-4 94-95 0.0013114718817065813 96-97 0.0017150016914624527 98-99 0.0015973054969503234 100-101 0.0010088245243896781 102-103 0.0011265207189018072 104-105 7.734321353654198E-4 106-107 5.884809725606455E-4 108-109 0.0010928932347554843 110-111 8.911283298775489E-4 112-113 0.01124839344694491 114-115 0.021420707401207495 116-117 0.019756146935964527 118-119 0.011483785835969167 120-121 0.006406035729874455 122-123 0.006792751797557166 124-125 0.005884809725606455 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2973758.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.03603211210568 #Duplication Level Percentage of deduplicated Percentage of total 1 42.6601464065914 12.386813809689176 2 19.809531389028123 11.503803790751721 3 12.87900687249859 11.218657713656963 4 8.421316100945983 9.780864189330405 5 5.1716078960279335 7.508148647014318 6 3.0915772259235674 5.386028136538279 7 1.9137740145296593 3.8897882618837616 8 1.2333277065883508 2.8648754314599203 9 0.8659509755999628 2.2629402301526786 >10 3.547513295418107 18.007690507138932 >50 0.2552628072081336 5.159791099198681 >100 0.1424356619572679 7.586343103234194 >500 0.006909989223360587 1.3854610191571284 >1k 0.0012883030755154806 0.44347947155327133 >5k 3.513553842314947E-4 0.6153145892404641 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7353 0.24726289092791004 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5435 0.18276537633526332 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5306 0.1784274308803877 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.3627484146322596E-5 14-15 0.0 0.0 0.0 0.0 1.68137420731613E-4 16-17 0.0 0.0 0.0 0.0 1.68137420731613E-4 18-19 0.0 0.0 0.0 0.0 1.68137420731613E-4 20-21 0.0 0.0 0.0 0.0 1.68137420731613E-4 22-23 0.0 0.0 0.0 3.3627484146322596E-5 1.68137420731613E-4 24-25 0.0 0.0 0.0 6.725496829264519E-5 1.68137420731613E-4 26-27 0.0 0.0 0.0 1.1769619451212909E-4 1.68137420731613E-4 28-29 1.6813742073161298E-5 0.0 0.0 3.194610993900647E-4 1.68137420731613E-4 30-31 3.3627484146322596E-5 0.0 0.0 0.0014291680762187104 1.68137420731613E-4 32-33 3.3627484146322596E-5 0.0 0.0 0.00479191649085097 1.68137420731613E-4 34-35 3.3627484146322596E-5 0.0 0.0 0.009651087949994586 1.68137420731613E-4 36-37 3.3627484146322596E-5 0.0 0.0 0.01952075454694027 1.68137420731613E-4 38-39 3.3627484146322596E-5 0.0 0.0 0.03672121268778428 1.68137420731613E-4 40-41 3.3627484146322596E-5 0.0 0.0 0.05316505243533603 1.68137420731613E-4 42-43 3.3627484146322596E-5 0.0 0.0 0.07488840719386042 1.68137420731613E-4 44-45 6.725496829264519E-5 0.0 0.0 0.10224436554689387 1.68137420731613E-4 46-47 6.725496829264519E-5 0.0 0.0 0.13967175540175092 1.68137420731613E-4 48-49 6.725496829264519E-5 0.0 0.0 0.17748586132429067 1.68137420731613E-4 50-51 6.725496829264519E-5 0.0 0.0 0.21259295477305146 1.68137420731613E-4 52-53 6.725496829264519E-5 0.0 0.0 0.25260966090717535 1.68137420731613E-4 54-55 6.725496829264519E-5 0.0 0.0 0.3025969160906839 1.68137420731613E-4 56-57 6.725496829264519E-5 0.0 0.0 0.36970056070467067 1.68137420731613E-4 58-59 6.725496829264519E-5 0.0 0.0 0.4487419621906019 1.68137420731613E-4 60-61 6.725496829264519E-5 0.0 0.0 0.5233109082850723 1.68137420731613E-4 62-63 6.725496829264519E-5 0.0 0.0 0.5955259304893001 1.68137420731613E-4 64-65 6.725496829264519E-5 0.0 0.0 0.6806707203477889 1.68137420731613E-4 66-67 6.725496829264519E-5 0.0 0.0 0.7917086729989462 1.68137420731613E-4 68-69 6.725496829264519E-5 0.0 0.0 0.9316998895000871 1.68137420731613E-4 70-71 6.725496829264519E-5 0.0 0.0 1.068866397332937 1.68137420731613E-4 72-73 6.725496829264519E-5 0.0 0.0 1.2031241277871298 1.68137420731613E-4 74-75 6.725496829264519E-5 0.0 0.0 1.3495550075022917 1.68137420731613E-4 76-77 6.725496829264519E-5 0.0 0.0 1.5252249846826809 1.68137420731613E-4 78-79 6.725496829264519E-5 0.0 0.0 1.746191183008167 2.522061310974195E-4 80-81 6.725496829264519E-5 0.0 0.0 1.9693767952873098 4.2034355182903244E-4 82-83 6.725496829264519E-5 0.0 0.0 2.193134074796941 5.04412262194839E-4 84-85 6.725496829264519E-5 0.0 0.0 2.425903520057785 5.04412262194839E-4 86-87 6.725496829264519E-5 0.0 0.0 2.6872899543271513 5.716672304874842E-4 88-89 6.725496829264519E-5 0.0 0.0 3.000412272955634 6.389221987801294E-4 90-91 6.725496829264519E-5 3.3627484146322596E-5 0.0 3.334181866849959 6.389221987801294E-4 92-93 6.725496829264519E-5 3.3627484146322596E-5 0.0 3.655980076388193 6.389221987801294E-4 94-95 6.725496829264519E-5 3.3627484146322596E-5 0.0 3.9950796265197104 6.389221987801294E-4 96-97 6.725496829264519E-5 3.3627484146322596E-5 0.0 4.367907543249989 6.389221987801294E-4 98-99 6.725496829264519E-5 3.3627484146322596E-5 0.0 4.792824432922921 6.389221987801294E-4 100-101 6.725496829264519E-5 3.3627484146322596E-5 0.0 5.260465041203757 6.389221987801294E-4 102-103 6.725496829264519E-5 3.3627484146322596E-5 0.0 5.722204026016913 7.734321353654198E-4 104-105 6.725496829264519E-5 3.3627484146322596E-5 0.0 6.199445281021522 7.734321353654198E-4 106-107 6.725496829264519E-5 3.3627484146322596E-5 0.0 6.702915973660264 7.734321353654198E-4 108-109 6.725496829264519E-5 6.725496829264519E-5 0.0 7.250354601820323 7.734321353654198E-4 110-111 6.725496829264519E-5 6.725496829264519E-5 0.0 7.823165166768782 9.583832981701941E-4 112-113 6.725496829264519E-5 6.725496829264519E-5 0.0 8.38442469091298 0.0010424520085360006 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5635 0.0 77.58982 1 GTATCAA 8430 0.0 51.550724 2 GTACATG 15080 0.0 47.321453 1 TACATGG 15635 0.0 45.0429 2 ACATGGG 15350 0.0 44.987213 3 TCAACGC 11750 0.0 38.099518 5 ATCAACG 11775 0.0 38.06918 4 CAACGCA 12080 0.0 37.282536 6 AACGCAG 12330 0.0 36.671364 7 CATGGGA 9160 0.0 36.589157 4 CATGGGG 6975 0.0 36.017014 4 TATCAAC 12685 0.0 35.90132 3 ACGCAGA 14440 0.0 31.271671 8 CGCAGAG 14775 0.0 30.2405 9 ATGGGGG 4040 0.0 28.29172 5 GAGTACA 10035 0.0 28.195435 1 ATGGGAT 4140 0.0 28.18352 5 AGTACAT 9650 0.0 26.64992 2 CAGAGTA 16550 0.0 26.565804 9 GCAGAGT 16855 0.0 25.767403 8 >>END_MODULE