FastQCFastQC Report
Thu 26 May 2016
SRR936186_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936186_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888800
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG36730.12714621988368874No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30300.063.811991
GTACATG162300.052.6011161
TACATGG160200.051.9546362
ACATGGG163850.049.163333
CATGGGA92650.044.8849564
GTATCAA45500.041.7107852
TCAACGC49900.038.8674555
CATGGGG70200.037.628334
CAACGCA51700.037.514896
ATCAACG51750.037.4779934
AACGCAG55100.035.092637
ATGGGAT43900.032.795935
TATCAAC60850.031.5799183
GAGTACA93650.029.8573761
ATGGGAG44250.028.503075
ACGCAGA66650.028.386458
ATGGGGG39800.027.8034675
CGCAGAG67850.027.3582849
AGTACAT101450.026.0375442
GTCGGGA5900.025.2090852
Produced by FastQC (version 0.11.5)