FastQCFastQC Report
Thu 26 May 2016
SRR936186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888800
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67460.2335225699252285No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45510.1575394627527001No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43770.15151620049847686No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG40610.14057740238161176No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56650.081.9406741
GTATCAA90100.051.275232
GTACATG147600.047.113621
TACATGG147450.046.271172
ACATGGG149250.044.79493
CATGGGA85550.040.918574
ATCAACG121950.039.5915224
TCAACGC122050.039.5590825
CAACGCA123150.039.277256
AACGCAG127600.038.093987
TATCAAC130400.036.8433463
CATGGGG67550.034.7243424
ATGGGAT39650.033.0325935
ACGCAGA149350.032.1877828
CGCAGAG151400.031.6733599
GAGTACA93850.030.9847161
AGTACAT99650.027.3626922
ATGGGAG40900.027.2195845
ATGGGGG36400.026.3322325
CAGAGTA167300.026.0315539
Produced by FastQC (version 0.11.5)