FastQCFastQC Report
Thu 26 May 2016
SRR936185_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936185_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238587
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15300.6412755095625495No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6350.2661502931844568No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5420.22717080142673324No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT4370.18316169782930336No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3430.14376307175160424No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT3240.1357995196720693No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG2930.12280635575282811No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG2510.10520271431385617No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4850.061.3296171
GGATAGC401.768798E-459.50228
CGTATAG709.121286E-750.9911961
CGCAAAG350.00779404250.9911962
GTACATG19800.049.875231
AAAGACG255.295505E-447.66171318-19
GGGTATG751.4655052E-647.6017657
GGTATGC505.323083E-447.601768
GAACAAA3550.046.9214741
TACATGG21250.046.4719772
ACATGGG21150.043.3164023
GGATATG705.444482E-542.4926641
GTATCAA7150.042.4332352
CATGGGG6950.040.2304654
ATGGGTA1850.038.596025
TCAACGC8150.037.234515
TCCTCGA401.1976357E-437.18108478-79
TGGCGGG650.001935472636.616749
GCTAGGC650.001937466236.6090662
CAACGCA8650.036.4579966