##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936185_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 238587 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.33427219421008 31.0 28.0 33.0 15.0 34.0 2 28.552142405076555 31.0 28.0 34.0 16.0 34.0 3 28.694111581938664 31.0 28.0 34.0 16.0 34.0 4 31.79829160851178 35.0 32.0 37.0 17.0 37.0 5 32.42907618604534 35.0 33.0 37.0 24.0 37.0 6 32.0564909236463 35.0 33.0 37.0 17.0 37.0 7 32.14930402746168 35.0 33.0 37.0 17.0 37.0 8 31.881607128636514 35.0 32.0 37.0 17.0 37.0 9 32.96185458553903 38.0 33.0 39.0 12.0 39.0 10-11 33.015453482377495 38.0 32.5 39.0 13.0 39.0 12-13 33.057513611387044 38.0 33.0 39.0 13.5 39.0 14-15 34.05034012750066 39.0 33.0 41.0 14.0 41.0 16-17 34.096864037017944 39.0 33.0 41.0 16.5 41.0 18-19 34.071003449475455 39.0 33.0 41.0 16.5 41.0 20-21 34.16661217920507 39.0 33.0 41.0 18.0 41.0 22-23 34.01801858441574 39.0 32.5 41.0 17.5 41.0 24-25 33.95535381223621 39.0 32.0 41.0 18.0 41.0 26-27 34.021281545096755 39.0 33.0 41.0 18.0 41.0 28-29 33.896071454018866 39.0 32.5 41.0 18.0 41.0 30-31 33.79669889809587 38.0 32.0 40.0 17.0 41.0 32-33 33.58921902702159 38.0 32.0 40.0 16.0 41.0 34-35 33.526803639762434 38.0 32.0 40.0 15.5 41.0 36-37 33.38957068071605 38.0 32.0 40.0 11.5 41.0 38-39 33.11448863517292 38.0 31.0 40.0 9.0 41.0 40-41 33.19218356406678 38.0 31.5 40.0 9.0 41.0 42-43 33.068635340567596 38.0 31.0 40.0 8.0 41.0 44-45 32.93916055778395 38.0 31.0 40.0 6.0 41.0 46-47 32.906095051281085 38.0 31.0 40.0 2.0 41.0 48-49 32.80619857745812 38.0 31.0 40.0 2.0 41.0 50-51 32.65661163433045 38.0 31.0 40.0 2.0 41.0 52-53 32.51868081664131 38.0 31.0 40.0 2.0 41.0 54-55 32.36380020705235 38.0 31.0 40.0 2.0 41.0 56-57 32.15248735262189 37.5 30.0 40.0 2.0 41.0 58-59 31.917510174485617 37.0 30.0 40.0 2.0 41.0 60-61 31.704072309052883 37.0 30.0 40.0 2.0 41.0 62-63 31.436056868144533 36.0 30.0 40.0 2.0 41.0 64-65 31.146663481245838 36.0 30.0 39.5 2.0 41.0 66-67 30.850046314342357 35.5 30.0 39.0 2.0 41.0 68-69 30.590673423111905 35.0 29.5 39.0 2.0 40.5 70-71 30.26769899449677 35.0 29.0 38.5 2.0 40.0 72-73 29.925863521482732 35.0 29.0 38.0 2.0 40.0 74-75 29.584933378599843 35.0 29.0 37.0 2.0 39.5 76-77 29.254812709829118 34.0 29.0 37.0 2.0 39.0 78-79 28.915705801238126 34.0 28.5 36.0 2.0 39.0 80-81 28.606032600267405 34.0 28.0 36.0 2.0 37.5 82-83 28.288096166178374 34.0 28.0 35.5 2.0 37.0 84-85 28.24110282622272 34.0 28.0 35.0 2.0 37.0 86-87 28.12314375888041 34.0 28.0 35.0 2.0 36.5 88-89 27.92913486485014 34.0 28.0 35.0 2.0 36.0 90-91 27.73321052697758 34.0 28.0 35.0 2.0 36.0 92-93 27.596273057626778 34.0 27.0 35.0 2.0 36.0 94-95 27.459528390063163 34.0 27.0 35.0 2.0 35.0 96-97 27.334184176002886 34.0 27.0 35.0 2.0 35.0 98-99 27.239642143117607 34.0 27.0 35.0 2.0 35.0 100-101 27.14004954167662 34.0 26.5 35.0 2.0 35.0 102-103 26.200735999865877 32.5 25.0 34.5 2.0 35.0 104-105 26.80974026246191 34.0 25.0 35.0 2.0 35.0 106-107 26.921496141868587 34.0 25.0 35.0 2.0 35.0 108-109 26.863833737797954 34.0 25.0 35.0 2.0 35.0 110-111 26.735203510669066 34.0 25.0 35.0 2.0 35.0 112-113 26.708441784338625 34.0 25.0 35.0 2.0 35.0 114-115 26.594778843776066 34.0 25.0 35.0 2.0 35.0 116-117 26.451495680820834 34.0 25.0 35.0 2.0 35.0 118-119 26.24438883929133 34.0 24.5 35.0 2.0 35.0 120-121 26.038363364307358 34.0 24.0 35.0 2.0 35.0 122-123 25.75924924660606 34.0 21.5 35.0 2.0 35.0 124-125 23.821968506247195 31.5 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5732360321027095 1101 2 0.6851514729429979 1101 3 0.5918613885253876 1101 4 0.4510005545336355 1101 5 0.6424869830726649 1101 6 -0.027276305569664316 1101 7 0.07488127641623521 1101 8 -0.08128125020227372 1101 9 0.30453012652743183 1101 10-11 0.37326635386542506 1101 12-13 0.37555165130773105 1101 14-15 0.2877036261128154 1101 16-17 0.09520999560874088 1101 18-19 0.3710842811872439 1101 20-21 0.5560490149463817 1101 22-23 0.8884439922697496 1101 24-25 0.8346753485080072 1101 26-27 0.7437853660930003 1101 28-29 0.6871969147120254 1101 30-31 0.6289621306940703 1101 32-33 0.7851531688497317 1101 34-35 0.6961897509307207 1101 36-37 0.5148457657203807 1101 38-39 0.8162580597069109 1101 40-41 0.5774985115742126 1101 42-43 0.7069209760538868 1101 44-45 0.5653210904752726 1101 46-47 0.568739848330722 1101 48-49 0.7434251263258034 1101 50-51 0.7463391675159343 1101 52-53 0.5461018227202814 1101 54-55 0.8380348075052382 1101 56-57 0.6106003023933937 1101 58-59 0.2031854978116101 1101 60-61 0.701900516619709 1101 62-63 0.49761814443262153 1101 64-65 0.5876763837526937 1101 66-67 0.567790519722724 1101 68-69 0.3967216204441293 1101 70-71 0.3098552705064961 1101 72-73 0.2520984418572283 1101 74-75 0.10867835509192147 1101 76-77 -0.07365028868577639 1101 78-79 0.18016331620778203 1101 80-81 0.1220600885711356 1101 82-83 0.19404921009697418 1101 84-85 0.01930795206807545 1101 86-87 0.16236182068024974 1101 88-89 -0.06902082164904755 1101 90-91 -0.2931650121910856 1101 92-93 -0.23820627084900536 1101 94-95 -0.13242758672538102 1101 96-97 -0.2825203325038004 1101 98-99 -0.10592808124363984 1101 100-101 -0.2504683599095898 1101 102-103 -0.44301927406198516 1101 104-105 -0.4288070995633042 1101 106-107 -0.32898558093304686 1101 108-109 -0.4023497941473231 1101 110-111 -0.4650282677099682 1101 112-113 -0.49371936297087515 1101 114-115 -0.5060019445417083 1101 116-117 -0.7315942846427106 1101 118-119 -0.4880150262743399 1101 120-121 -0.27198099178312063 1101 122-123 -0.28495694288006845 1101 124-125 -0.2998232307673554 1102 1 0.5271380672504051 1102 2 -0.08666894263309288 1102 3 0.6627176462039301 1102 4 0.4909156231731302 1102 5 0.41030120239855705 1102 6 0.1838769447908959 1102 7 0.6123659193306068 1102 8 0.564076903742496 1102 9 0.7282521499236125 1102 10-11 0.7527933556281496 1102 12-13 0.6968152056050485 1102 14-15 0.7990812234633893 1102 16-17 1.044291241268695 1102 18-19 0.9126953062393213 1102 20-21 0.576994744341178 1102 22-23 0.8426531691299672 1102 24-25 0.8963774477548441 1102 26-27 0.7129042699977077 1102 28-29 0.9303930580804405 1102 30-31 0.6257037577439135 1102 32-33 0.8129294628511303 1102 34-35 0.3537534494510979 1102 36-37 0.7333036472438081 1102 38-39 0.5410924702619013 1102 40-41 0.9073420028407071 1102 42-43 0.6050567593987921 1102 44-45 0.7058451009447992 1102 46-47 0.790609673660569 1102 48-49 0.7677694455395283 1102 50-51 0.2921633756244759 1102 52-53 0.632348550993342 1102 54-55 0.4130374783027406 1102 56-57 0.517756704294996 1102 58-59 0.7191835748374089 1102 60-61 0.45505873466362345 1102 62-63 0.29307511788489293 1102 64-65 0.3107480679576007 1102 66-67 0.18021106349709726 1102 68-69 0.32069069260908023 1102 70-71 0.3907937887480415 1102 72-73 -0.31586000053367513 1102 74-75 0.023974012375184373 1102 76-77 0.05950899767713125 1102 78-79 0.012163476455629763 1102 80-81 0.02705314449763918 1102 82-83 0.002165763699878198 1102 84-85 0.06712190431421661 1102 86-87 0.20619628465119177 1102 88-89 0.13303836322355522 1102 90-91 0.31432791019554074 1102 92-93 0.3799231025819019 1102 94-95 0.10193489391133781 1102 96-97 0.40183814193666834 1102 98-99 0.47372177720409425 1102 100-101 0.20803412393208376 1102 102-103 0.20807441751432165 1102 104-105 0.2474989204142588 1102 106-107 0.2855515698601785 1102 108-109 0.43957237881352285 1102 110-111 0.20005372577328728 1102 112-113 -0.011286533528004128 1102 114-115 0.11996621955209008 1102 116-117 0.34998522499886775 1102 118-119 0.2955502213085879 1102 120-121 0.08113182270328068 1102 122-123 0.11735863855254891 1102 124-125 0.21670232876472184 1103 1 -0.13292704398128308 1103 2 -0.0611670435555034 1103 3 0.0037648518469026726 1103 4 0.2418922916484405 1103 5 -0.2699755203361178 1103 6 -0.26354573562147365 1103 7 -0.7425716824056892 1103 8 -0.5581151743909842 1103 9 -0.49602891355983303 1103 10-11 -0.6024426725867116 1103 12-13 -0.6308977623819274 1103 14-15 -0.6839898562978703 1103 16-17 -0.5067125568864839 1103 18-19 -0.23006106923545389 1103 20-21 -0.7067952719366275 1103 22-23 -0.47918622316520754 1103 24-25 -0.23327529234282451 1103 26-27 -0.11688411849931413 1103 28-29 0.02777504397300845 1103 30-31 0.1507851467835266 1103 32-33 0.2224384156454846 1103 34-35 0.1938104217788208 1103 36-37 -0.15695679768294468 1103 38-39 0.009133707375305278 1103 40-41 -0.2591799721999877 1103 42-43 -0.2297370550341924 1103 44-45 0.015137016300201367 1103 46-47 -0.05167193241648249 1103 48-49 -0.04561905147620848 1103 50-51 -0.17034340679548876 1103 52-53 -0.1615065765161532 1103 54-55 -0.3653944262007869 1103 56-57 -0.5286893076420647 1103 58-59 -0.2760822960253151 1103 60-61 -0.5143525512832063 1103 62-63 -0.2587290058264493 1103 64-65 -0.3903642489170629 1103 66-67 -0.5963081985756098 1103 68-69 -0.2269625900821879 1103 70-71 -0.2183353485281394 1103 72-73 -0.1542078030295997 1103 74-75 -0.30422728984945024 1103 76-77 -0.36443294480794464 1103 78-79 -0.4802266483408957 1103 80-81 -0.6053396932668775 1103 82-83 -0.48265518041297995 1103 84-85 -0.5666274174437866 1103 86-87 -0.5640153268517913 1103 88-89 -0.23532843004828408 1103 90-91 -0.7711170165543244 1103 92-93 -0.6040025620952534 1103 94-95 -0.7779484484652208 1103 96-97 -0.5446078700003909 1103 98-99 -0.6985923844888013 1103 100-101 -0.7075654419930402 1103 102-103 -0.7426852135220336 1103 104-105 -0.638597118652477 1103 106-107 -0.7521993796786184 1103 108-109 -1.1010271871115975 1103 110-111 -0.9509636372218289 1103 112-113 -0.797803082632722 1103 114-115 -0.8444938998185023 1103 116-117 -0.9341167825974424 1103 118-119 -0.8750412057125736 1103 120-121 -0.8865160340520077 1103 122-123 -1.0265236186368156 1103 124-125 -1.0386015236498203 1104 1 0.16115868580710568 1104 2 0.1968362120060796 1104 3 0.06735682146166155 1104 4 0.3105032520391049 1104 5 0.19896099621298902 1104 6 -0.15890656036374295 1104 7 0.08659272345431646 1104 8 0.1413964913713599 1104 9 0.12515936641845826 1104 10-11 0.24892737624670502 1104 12-13 0.11691829838855483 1104 14-15 -0.061743518804661335 1104 16-17 0.05460051961921408 1104 18-19 -0.15044902365759327 1104 20-21 0.3385926824855119 1104 22-23 0.41997275675883117 1104 24-25 0.09483105600226338 1104 26-27 -0.06029160629095287 1104 28-29 0.024465223028890648 1104 30-31 -0.2261003659674259 1104 32-33 -0.05949321756123993 1104 34-35 6.60665945943606E-4 1104 36-37 0.24619024518194976 1104 38-39 0.5681543259320065 1104 40-41 0.2771873636654476 1104 42-43 0.5264485119761133 1104 44-45 0.544762626717997 1104 46-47 0.46071276644407533 1104 48-49 0.2858106826529223 1104 50-51 0.22128708696468635 1104 52-53 0.35657535511705163 1104 54-55 0.21200655046768446 1104 56-57 0.22394227130530453 1104 58-59 0.28232790473431635 1104 60-61 0.08134739445743122 1104 62-63 0.006992643658090714 1104 64-65 0.14322229476172055 1104 66-67 0.31680900164142756 1104 68-69 0.15574223900082984 1104 70-71 0.19234018050061863 1104 72-73 0.3658410303941615 1104 74-75 0.24433483711745652 1104 76-77 0.20899353375960317 1104 78-79 0.1101016207855281 1104 80-81 0.19844489707496038 1104 82-83 0.5524234956586405 1104 84-85 0.4794930383348337 1104 86-87 0.44588700630067635 1104 88-89 0.6678321776565426 1104 90-91 0.5901011060718275 1104 92-93 0.6492529994891179 1104 94-95 0.5864709763855664 1104 96-97 0.6015804099779025 1104 98-99 0.6631547668948166 1104 100-101 0.807260928055797 1104 102-103 0.6011156546277228 1104 104-105 0.6224989204142624 1104 106-107 0.8435412605818264 1104 108-109 0.9163765025248658 1104 110-111 0.9152599113402964 1104 112-113 0.728404188121484 1104 114-115 0.8841414772840466 1104 116-117 0.5510161528339168 1104 118-119 0.597096613061165 1104 120-121 0.7782967711568922 1104 122-123 0.4652967828824437 1104 124-125 0.10330026690905214 1105 1 0.37706629379353274 1105 2 0.3811047752319716 1105 3 0.43670955537788103 1105 4 0.262878300975558 1105 5 0.13262274748533542 1105 6 0.2529099605810572 1105 7 0.20639567269090975 1105 8 0.044782893563713344 1105 9 0.33824823500778933 1105 10-11 0.16606165785965032 1105 12-13 0.6696685267586417 1105 14-15 0.40888156275609333 1105 16-17 0.40241469161450993 1105 18-19 0.19045858432884444 1105 20-21 -0.09377062170344885 1105 22-23 -0.22029215254561763 1105 24-25 0.062477539102872015 1105 26-27 -0.021197909305435303 1105 28-29 0.007122145905050559 1105 30-31 0.5481036011472824 1105 32-33 0.3642605342330967 1105 34-35 0.6894966309726911 1105 36-37 0.5823836420239203 1105 38-39 0.6214754795271986 1105 40-41 0.285490247653442 1105 42-43 0.7579211728139086 1105 44-45 0.8326753240949998 1105 46-47 0.7811062154849111 1105 48-49 0.8200205221413768 1105 50-51 0.5786406038640663 1105 52-53 0.49505571528930403 1105 54-55 0.510704840954439 1105 56-57 0.5147650561154293 1105 58-59 0.699850415500336 1105 60-61 0.9006707665049305 1105 62-63 0.8536127663254511 1105 64-65 0.38636792963056976 1105 66-67 0.7742114549886807 1105 68-69 0.7245697217099512 1105 70-71 0.6872442650628017 1105 72-73 0.7175277066306229 1105 74-75 0.6640105515895911 1105 76-77 0.22760569609811654 1105 78-79 0.5932576954299229 1105 80-81 0.7002066091981476 1105 82-83 0.6249897229847541 1105 84-85 0.3711640549079789 1105 86-87 0.3073870715492717 1105 88-89 0.14311927148402503 1105 90-91 0.06489231056093203 1105 92-93 -0.07870276196201331 1105 94-95 -0.18192912801218952 1105 96-97 0.04315029122545866 1105 98-99 0.42715711584431304 1105 100-101 0.261671733223487 1105 102-103 0.13371385376644085 1105 104-105 -0.022907578346014645 1105 106-107 0.04375331852258313 1105 108-109 0.1476403678517606 1105 110-111 0.17163796171221435 1105 112-113 0.24698372616141384 1105 114-115 0.26473328206024505 1105 116-117 0.15179261114006692 1105 118-119 0.41414933392766784 1105 120-121 0.9072214742757758 1105 122-123 0.48325319681954326 1105 124-125 0.6017049387086111 1106 1 -0.07374924874692823 1106 2 -0.22462606424154785 1106 3 -0.00779576611591537 1106 4 0.060195512386854944 1106 5 0.0959579701064115 1106 6 0.0707826225874797 1106 7 -0.22772486386680768 1106 8 0.043573749411056184 1106 9 -0.06056151371694796 1106 10-11 0.3796141485706599 1106 12-13 0.11237272727504433 1106 14-15 -0.21897283613552787 1106 16-17 -0.01810297322583665 1106 18-19 0.1364092109426096 1106 20-21 0.2774807165420299 1106 22-23 0.024924800663022495 1106 24-25 -4.15780396477016 1106 26-27 -1.2645602117174306 1106 28-29 -0.629019928532891 1106 30-31 -0.8215701818365346 1106 32-33 -5.829977138164416 1106 34-35 -1.025009950046261 1106 36-37 -0.12173815405895994 1106 38-39 0.19035261284793847 1106 40-41 -0.42540149615913947 1106 42-43 0.17909379073694964 1106 44-45 -0.05983679883465243 1106 46-47 0.1848690468974752 1106 48-49 0.1789096209511314 1106 50-51 0.3862355405729332 1106 52-53 0.2717482022217368 1106 54-55 0.051488265603346406 1106 56-57 0.2544149081212197 1106 58-59 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-0.28022023389662465 2216 64-65 0.12089306102445718 2216 66-67 0.2618789359273137 2216 68-69 0.22713097716927777 2216 70-71 0.13327283860636285 2216 72-73 -0.28271657495943003 2216 74-75 0.11901832418563885 2216 76-77 0.17299395577684606 2216 78-79 0.23585522300412975 2216 80-81 -0.047591155255179984 2216 82-83 0.223046573972983 2216 84-85 0.4238696585796049 2216 86-87 0.4425636205166299 2216 88-89 0.5602856050394323 2216 90-91 0.8850233343228702 2216 92-93 0.7359097186065 2216 94-95 0.8353556451020303 2216 96-97 0.7388716621633513 2216 98-99 0.9572555687275752 2216 100-101 0.8481363123929277 2216 102-103 1.1059989970042814 2216 104-105 1.0669584369030787 2216 106-107 0.8005783980991588 2216 108-109 1.022657023414716 2216 110-111 1.2509369475734893 2216 112-113 1.0543898998234162 2216 114-115 0.8718803691873447 2216 116-117 1.4062340945955398 2216 118-119 1.259023121772806 2216 120-121 0.9643611589304477 2216 122-123 1.02449977317605 2216 124-125 1.403211341847257 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10778.0 3 1905.0 4 2032.0 5 2234.0 6 2618.0 7 1580.0 8 1288.0 9 1274.0 10 1370.0 11 1556.0 12 1654.0 13 1609.0 14 1627.0 15 1619.0 16 1544.0 17 1364.0 18 1215.0 19 1071.0 20 1089.0 21 1082.0 22 1238.0 23 1736.0 24 2315.0 25 3161.0 26 4036.0 27 3627.0 28 4101.0 29 4766.0 30 5585.0 31 7088.0 32 8947.0 33 11713.0 34 15967.0 35 22405.0 36 35830.0 37 49317.0 38 16233.0 39 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.14803948278391 20.512605570341808 10.097868684116772 21.24148626275751 2 18.374429453406936 24.342483035538397 35.783173433590264 21.499914077464403 3 15.629937926207212 33.32620804989375 28.923621152870858 22.12023287102818 4 10.329232768195821 19.97443259215793 36.08986315149737 33.60647148814888 5 10.097868684116772 45.946308443531656 33.30385397237882 10.651968899972756 6 27.129534547435924 42.459500806840325 17.658696062200054 12.75226858352369 7 25.510405096716056 38.47852966448016 21.444348974160153 14.566716264643627 8 25.566257586426584 37.24851624585052 19.593937564966637 17.59128860275626 9 26.065671341509418 18.139256104819307 18.40834597748363 37.38672657618764 10-11 22.08208900485157 31.289176699883686 27.95448115431769 18.67425314094705 12-13 23.115742176693967 31.239573815292015 26.837104224124204 18.807579783889818 14-15 21.49371906161062 32.42406079328021 23.72840861936717 22.353811525742 16-17 20.859128422870413 33.51866677285092 25.197314097217273 20.424890707061394 18-19 20.430592027362064 32.50292880540368 26.916507564596138 20.149971602638125 20-21 22.14265355565056 31.69236056970886 26.49846171901852 19.66652415562206 22-23 21.972229796337857 31.623214547895213 26.302619711810728 20.101935943956207 24-25 21.150017394657535 31.767045716513188 26.859027827260572 20.223909061568705 26-27 20.429496838606514 31.925149267650678 26.85350708248544 20.79184681125737 28-29 20.81816562405038 30.99451971540243 27.25346578227657 20.93384887827062 30-31 20.990135529070535 31.9200615298208 26.778465401002165 20.311337540106503 32-33 20.62963809012853 32.27094305300285 26.599715401236057 20.499703455632563 34-35 21.070985481004595 31.81919156355833 26.630453006069143 20.479369949367936 36-37 20.922225109604085 31.98888451124542 26.233727041821396 20.855163337329095 38-39 20.377433619045622 32.362470398390506 26.942808642622122 20.31728733994174 40-41 20.46126118574093 31.996353500848755 27.530439885156234 20.011945428254084 42-43 20.44810589231375 32.09860760660905 26.46574343412328 20.987543066953922 44-45 20.21053425739835 32.525509936772664 26.462643057881202 20.801312747947783 46-47 20.46038485558485 31.84740740119791 26.721308726941988 20.970899016275258 48-49 20.144643253823553 32.59775260177629 26.520724096451186 20.736880047948965 50-51 20.57179022901787 31.70518559501056 26.656020521074336 21.067003654897228 52-53 20.482518552360514 31.930388567428743 26.5005878494351 21.086505030775644 54-55 20.54455186173278 32.01798965971796 26.71938100816702 20.718077470382237 56-57 20.788616097157167 32.10336047279243 26.285247241520228 20.822776188530174 58-59 20.475135643765626 31.489891422116695 26.64129480330868 21.393678130809 60-61 21.000945157795247 32.10854016082352 26.25359149483726 20.63692318654397 62-63 20.330159714814435 31.99263144879213 26.90380436035485 20.773404476038586 64-65 20.58625054488147 31.399842403514068 26.881727190423497 21.132179861180965 66-67 20.670237691072856 32.112604626404625 26.052341493878544 21.164816188643975 68-69 20.49533501546612 31.710368588265865 26.627505385898587 21.166791010369426 70-71 20.91770228639688 31.165203177064775 26.85772366242639 21.059370874111952 72-73 20.746397525420605 31.83149891443756 26.01703369015785 21.40506986998399 74-75 20.586583397950417 32.05147012595092 26.501037366137854 20.860909109960808 76-77 20.445543516985563 31.29136366494122 27.341199153341577 20.92189366473165 78-79 20.276251430295865 31.6285044868329 26.924769580907594 21.170474501963643 80-81 20.515329966259404 32.07871408512689 26.426221262862292 20.97973468575141 82-83 21.12562712972626 31.225716405336417 26.2790931609853 21.36956330395202 84-85 20.368546352258722 31.219979378505023 26.68073566764186 21.730738601594393 86-87 20.672629575918116 31.6483364489115 26.72390668354388 20.9551272916265 88-89 20.90776491495729 30.960743715054527 26.98272321091766 21.148768159070524 90-91 20.665417646393138 31.188203883698606 26.973808296344732 21.17257017356352 92-93 20.709390783159044 31.348995117044243 26.60330699750613 21.33830710229059 94-95 20.585658839998995 30.754738333347305 27.1072066257031 21.5523962009506 96-97 20.666342534767338 31.128398145741997 26.895542906960173 21.30971641253049 98-99 20.12515350794469 31.277269926693407 27.03751671298101 21.560059852380892 100-101 19.973217316953566 31.433607028044275 26.802172792314753 21.791002862687407 102-103 20.597524147629038 31.53536797235361 26.526241226902837 21.340866653114514 104-105 19.62365929409398 32.631702481694305 26.420550993977038 21.324087230234674 106-107 19.683049298785342 32.294225143134966 26.970358696654458 21.05236686142523 108-109 20.060984554770837 32.51294088060859 26.023848942724815 21.40222562189576 110-111 19.98805457174592 33.185657103338436 25.57055137581994 21.25573694909571 112-113 19.53777770326485 32.69673682291328 26.660351696344932 21.10513377747693 114-115 20.112663777421975 32.6743815647188 26.092897320043924 21.120057337815297 116-117 19.6652360155248 32.89317059676595 26.14109796886657 21.300495418842683 118-119 19.45616866106419 32.16568518557328 26.453465222038268 21.924680931324268 120-121 19.623281283727287 32.20404339725844 26.432970851242633 21.73970446777165 122-123 19.631495561348945 32.163454687198744 26.504488947381656 21.700560804070648 124-125 20.152649039237343 32.01166034121796 26.003357272938743 21.83233334660595 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 865.0 1 1086.0 2 1274.0 3 1045.5 4 715.5 5 520.0 6 412.5 7 350.0 8 334.0 9 330.5 10 291.0 11 252.0 12 239.0 13 232.0 14 217.5 15 211.0 16 233.0 17 269.0 18 303.0 19 323.0 20 378.5 21 489.5 22 564.0 23 630.0 24 741.0 25 948.0 26 1311.5 27 1704.0 28 2058.0 29 2590.0 30 3329.5 31 4003.0 32 5028.5 33 6513.0 34 7746.5 35 8571.5 36 9773.5 37 10791.0 38 10405.5 39 10074.0 40 9468.5 41 8486.5 42 7968.5 43 7785.0 44 8145.0 45 8301.0 46 7614.5 47 6881.0 48 6816.5 49 6482.0 50 6246.5 51 6086.5 52 5731.5 53 5522.0 54 5263.5 55 4909.5 56 4442.0 57 4204.0 58 3908.0 59 3269.5 60 2802.5 61 2368.0 62 1940.0 63 1463.5 64 973.5 65 773.0 66 669.5 67 598.5 68 500.0 69 423.5 70 368.5 71 276.0 72 233.0 73 217.0 74 197.5 75 159.5 76 107.0 77 74.5 78 43.0 79 31.5 80 31.5 81 22.5 82 13.5 83 7.5 84 4.5 85 4.5 86 4.5 87 4.5 88 3.5 89 2.5 90 2.0 91 1.5 92 2.5 93 2.0 94 2.0 95 3.0 96 1.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.38268639951045E-4 2 0.0 3 0.0 4 8.38268639951045E-4 5 8.38268639951045E-4 6 8.38268639951045E-4 7 8.38268639951045E-4 8 0.0012574029599265676 9 0.0020956715998776128 10-11 0.0018861044398898515 12-13 0.0020956715998776128 14-15 0.00335307455980418 16-17 0.00335307455980418 18-19 0.0035626417197919414 20-21 0.003772208879779703 22-23 0.08466513263505555 24-25 0.00335307455980418 26-27 0.002305238759865374 28-29 0.0018861044398898515 30-31 0.0014669701199143288 32-33 0.002305238759865374 34-35 0.0012574029599265676 36-37 4.191343199755225E-4 38-39 8.38268639951045E-4 40-41 8.38268639951045E-4 42-43 0.0020956715998776128 44-45 0.0014669701199143288 46-47 0.00167653727990209 48-49 0.0 50-51 0.0012574029599265676 52-53 0.002305238759865374 54-55 0.002305238759865374 56-57 0.0018861044398898515 58-59 0.0010478357999388064 60-61 0.0010478357999388064 62-63 0.002305238759865374 64-65 0.0012574029599265676 66-67 0.0 68-69 4.191343199755225E-4 70-71 8.38268639951045E-4 72-73 4.191343199755225E-4 74-75 8.38268639951045E-4 76-77 8.38268639951045E-4 78-79 0.0 80-81 8.38268639951045E-4 82-83 0.0 84-85 4.191343199755225E-4 86-87 4.191343199755225E-4 88-89 4.191343199755225E-4 90-91 0.0 92-93 8.38268639951045E-4 94-95 4.191343199755225E-4 96-97 4.191343199755225E-4 98-99 0.0 100-101 0.0 102-103 0.0010478357999388064 104-105 0.0 106-107 4.191343199755225E-4 108-109 8.38268639951045E-4 110-111 8.38268639951045E-4 112-113 2.0956715998776126E-4 114-115 4.191343199755225E-4 116-117 4.191343199755225E-4 118-119 8.38268639951045E-4 120-121 2.0956715998776126E-4 122-123 4.191343199755225E-4 124-125 2.0956715998776126E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 238587.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.65827140623756 #Duplication Level Percentage of deduplicated Percentage of total 1 62.515868164934886 22.292077942218143 2 11.369834030748978 8.108572554246459 3 7.462739197893647 7.983251392573779 4 5.088391555785416 7.257729884696149 5 3.5403639099158397 6.31216285883137 6 2.36024260661056 5.0497302870650955 7 1.6103248859842965 4.019498128565261 8 1.1060698669424984 3.1552431607757336 9 0.8333725139874935 2.6744960957638098 >10 3.8577272086134746 23.529781589105863 >50 0.17983920259532654 4.400910359742987 >100 0.0717005971131694 4.081949142241614 >500 0.0023508392496121113 0.49332109461119 >1k 0.0011754196248060557 0.6412755095625495 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1530 0.6412755095625495 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 635 0.2661502931844568 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 542 0.22717080142673324 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 437 0.18316169782930336 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 343 0.14376307175160424 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 324 0.1357995196720693 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 293 0.12280635575282811 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 251 0.10520271431385617 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.095671599877613E-4 0.0 20-21 0.0 0.0 0.0 0.0010478357999388064 0.0 22-23 0.0 0.0 0.0 0.0016765372799020903 0.0 24-25 0.0 0.0 0.0 0.002305238759865374 0.0 26-27 0.0 0.0 0.0 0.0029339402398286577 0.0 28-29 0.0 0.0 0.0 0.0035626417197919414 0.0 30-31 0.0 0.0 0.0 0.010059223679412543 0.0 32-33 0.0 0.0 0.0 0.02116628315876389 0.0 34-35 0.0 0.0 0.0 0.03562641719791942 4.191343199755226E-4 36-37 0.0 0.0 0.0 0.0557448645567445 4.191343199755226E-4 38-39 0.0 0.0 0.0 0.0892756101547863 4.191343199755226E-4 40-41 0.0 0.0 0.0 0.12469246019271796 4.191343199755226E-4 42-43 0.0 0.0 0.0 0.17247377266992753 4.191343199755226E-4 44-45 0.0 0.0 0.0 0.2217220552670514 4.191343199755226E-4 46-47 0.0 0.0 0.0 0.27830518846374697 4.191343199755226E-4 48-49 0.0 0.0 0.0 0.3359361574603813 4.191343199755226E-4 50-51 0.0 0.0 0.0 0.390633186217187 4.191343199755226E-4 52-53 0.0 0.0 0.0 0.45266506557356434 4.191343199755226E-4 54-55 0.0 0.0 0.0 0.533767556488828 4.191343199755226E-4 56-57 0.0 0.0 0.0 0.6211570622037244 4.191343199755226E-4 58-59 0.0 0.0 0.0 0.730341552557348 4.191343199755226E-4 60-61 0.0 0.0 0.0 0.8368016698311308 4.191343199755226E-4 62-63 0.0 0.0 0.0 0.9503870705444974 4.191343199755226E-4 64-65 0.0 0.0 0.0 1.0840909186166892 4.191343199755226E-4 66-67 0.0 0.0 0.0 1.2391706170076324 4.191343199755226E-4 68-69 0.0 0.0 0.0 1.4145783299173886 4.191343199755226E-4 70-71 0.0 0.0 0.0 1.6103140573459576 4.191343199755226E-4 72-73 0.0 0.0 0.0 1.8054210832945634 4.191343199755226E-4 74-75 0.0 0.0 0.0 2.010377765762594 4.191343199755226E-4 76-77 0.0 0.0 0.0 2.2536852385083845 4.191343199755226E-4 78-79 0.0 0.0 0.0 2.5192068302128785 4.191343199755226E-4 80-81 0.0 0.0 0.0 2.820983540595255 4.191343199755226E-4 82-83 0.0 0.0 0.0 3.119616743577814 4.191343199755226E-4 84-85 0.0 0.0 0.0 3.4463319459987343 4.191343199755226E-4 86-87 0.0 0.0 0.0 3.805739625377745 4.191343199755226E-4 88-89 0.0 0.0 0.0 4.183798781995666 4.191343199755226E-4 90-91 0.0 0.0 0.0 4.598113057291471 4.191343199755226E-4 92-93 0.0 0.0 0.0 5.0231152577466505 4.191343199755226E-4 94-95 0.0 0.0 0.0 5.466140233960777 4.191343199755226E-4 96-97 0.0 0.0 0.0 5.993830342809961 4.191343199755226E-4 98-99 0.0 0.0 0.0 6.5338849140984205 4.191343199755226E-4 100-101 0.0 0.0 0.0 7.111032872704715 4.191343199755226E-4 102-103 0.0 0.0 0.0 7.671625025671977 4.191343199755226E-4 104-105 0.0 0.0 0.0 8.273921043476804 4.191343199755226E-4 106-107 0.0 0.0 0.0 8.916663523159267 4.191343199755226E-4 108-109 0.0 0.0 0.0 9.605091643719062 4.191343199755226E-4 110-111 0.0 0.0 0.0 10.338576703676228 4.191343199755226E-4 112-113 0.0 0.0 0.0 11.095323718392034 4.191343199755226E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 485 0.0 61.329617 1 GGATAGC 40 1.768798E-4 59.5022 8 CGTATAG 70 9.121286E-7 50.991196 1 CGCAAAG 35 0.007794042 50.991196 2 GTACATG 1980 0.0 49.87523 1 AAAGACG 25 5.295505E-4 47.661713 18-19 GGGTATG 75 1.4655052E-6 47.601765 7 GGTATGC 50 5.323083E-4 47.60176 8 GAACAAA 355 0.0 46.921474 1 TACATGG 2125 0.0 46.471977 2 ACATGGG 2115 0.0 43.316402 3 GGATATG 70 5.444482E-5 42.492664 1 GTATCAA 715 0.0 42.433235 2 CATGGGG 695 0.0 40.230465 4 ATGGGTA 185 0.0 38.59602 5 TCAACGC 815 0.0 37.23451 5 TCCTCGA 40 1.1976357E-4 37.181084 78-79 TGGCGGG 65 0.0019354726 36.61674 9 GCTAGGC 65 0.0019374662 36.609066 2 CAACGCA 865 0.0 36.457996 6 >>END_MODULE