FastQCFastQC Report
Thu 26 May 2016
SRR936185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936185_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238587
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30641.2842275564050012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24781.0386148448993449No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23300.9765829655429676No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6000.25148059198531353No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.20956715998776126No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.20872889134781022No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT4810.20160360790822635No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.17519814574976844No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.15466056407096782No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA2780.11651934095319527No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG2620.10981319183358691No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT2510.10520271431385617No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT2450.10268790839400303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12750.078.529631
CGTAAGG300.004204834359.5983851
CGTTATA150.004233719359.50467106-107
CGTGAGG150.004235477759.4984362-63
CAACGCG150.004238995359.4859634-35
GTGGACG350.007795996550.9879659
GTATCAA19800.050.5258872
AGGTCCG302.1917816E-549.58202460-61
TGTCGTG255.33014E-447.59874350-51
CTAAGCG255.335653E-447.58876820-21
GGGATGA2300.046.5542267
GATGACC1700.045.489269
ATGGGAT4400.044.661275
TAGGACG558.480789E-443.30794
GGATGAC2300.041.3815358
GTACATG18700.041.1133231
GTCCAAT600.001295856439.7322581
GGCTTAT1351.2951205E-939.7322541
ATGGGGG3900.039.698915
GTAGGAC758.130096E-539.698913
Produced by FastQC (version 0.11.5)