##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936185_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 238587 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38937578325726 31.0 31.0 34.0 30.0 34.0 2 31.35152795416347 31.0 31.0 34.0 28.0 34.0 3 31.643216101464038 31.0 31.0 34.0 30.0 34.0 4 35.508615305947096 37.0 35.0 37.0 33.0 37.0 5 35.176145389312914 37.0 35.0 37.0 33.0 37.0 6 35.38971109071324 37.0 35.0 37.0 33.0 37.0 7 35.21157062203724 37.0 35.0 37.0 32.0 37.0 8 35.29233780549653 37.0 35.0 37.0 32.0 37.0 9 36.93037340676567 39.0 37.0 39.0 33.0 39.0 10-11 36.809989647382295 39.0 37.0 39.0 32.0 39.0 12-13 36.770582638618194 39.0 37.0 39.0 32.0 39.0 14-15 37.78376441298143 40.0 37.0 41.0 32.0 41.0 16-17 37.70730593033149 40.0 36.5 41.0 32.0 41.0 18-19 37.920609672781836 40.0 37.0 41.0 32.0 41.0 20-21 37.98959918184981 40.0 37.0 41.0 32.0 41.0 22-23 37.866518293117394 40.0 37.0 41.0 32.0 41.0 24-25 37.818724406610585 40.0 37.0 41.0 32.0 41.0 26-27 37.60242175810082 40.0 36.5 41.0 32.0 41.0 28-29 37.41119591595519 39.5 36.0 41.0 32.0 41.0 30-31 37.12646120702301 39.0 36.0 41.0 31.5 41.0 32-33 36.775679311949105 39.0 35.0 41.0 31.0 41.0 34-35 36.51732910845939 39.0 35.0 41.0 30.0 41.0 36-37 36.24068578757434 39.0 35.0 41.0 30.0 41.0 38-39 36.1712436134408 39.0 35.0 41.0 30.0 41.0 40-41 36.1164606621484 39.0 35.0 41.0 30.0 41.0 42-43 35.93174607166358 39.0 35.0 41.0 29.5 41.0 44-45 35.6643991499956 39.0 35.0 41.0 28.0 41.0 46-47 35.16793664365619 39.0 35.0 41.0 25.0 41.0 48-49 34.71902073457481 39.0 34.5 41.0 23.0 41.0 50-51 34.501527744596316 39.0 34.0 41.0 22.0 41.0 52-53 34.395258333438115 39.0 34.0 41.0 22.0 41.0 54-55 34.22513590430325 38.0 34.0 40.5 21.0 41.0 56-57 34.04146914961838 38.0 34.0 40.0 20.0 41.0 58-59 33.83113287815346 38.0 33.5 40.0 19.5 41.0 60-61 33.62926102428045 38.0 33.0 40.0 18.5 41.0 62-63 33.39981013215305 37.0 33.0 40.0 18.0 41.0 64-65 33.15493300137895 37.0 33.0 40.0 17.0 41.0 66-67 32.86700448892857 36.0 33.0 39.5 13.5 41.0 68-69 32.55229538910335 36.0 32.5 39.0 13.0 41.0 70-71 32.24212341829186 35.5 32.0 39.0 9.5 41.0 72-73 31.959842740803147 35.0 32.0 39.0 8.0 40.0 74-75 31.732988385787994 35.0 32.0 37.5 7.0 40.0 76-77 31.39829705725794 35.0 32.0 37.0 4.5 39.0 78-79 31.03645630315147 35.0 31.5 36.5 2.0 39.0 80-81 30.73477389799109 35.0 31.0 36.0 2.0 39.0 82-83 30.457814130694466 35.0 31.0 36.0 2.0 37.0 84-85 30.140994689568167 35.0 31.0 35.5 2.0 37.0 86-87 29.924589353150004 35.0 31.0 35.0 2.0 36.5 88-89 29.718236953396456 35.0 31.0 35.0 2.0 36.0 90-91 29.537472703877413 34.5 31.0 35.0 2.0 36.0 92-93 29.369779158126804 34.0 31.0 35.0 2.0 36.0 94-95 29.207961875542257 34.0 30.5 35.0 2.0 35.5 96-97 29.110852644947126 34.0 30.0 35.0 2.0 35.0 98-99 29.017568015021773 34.0 30.0 35.0 2.0 35.0 100-101 28.92821696068939 34.0 30.0 35.0 2.0 35.0 102-103 27.918534957897958 33.5 28.0 34.5 2.0 35.0 104-105 28.611454102696293 34.0 29.5 35.0 2.0 35.0 106-107 28.731458545520084 34.0 30.0 35.0 2.0 35.0 108-109 28.674955047844183 34.0 30.0 35.0 2.0 35.0 110-111 28.62246266561045 34.0 30.0 35.0 2.0 35.0 112-113 28.52828318391195 34.0 30.0 35.0 2.0 35.0 114-115 28.478318181627664 34.0 30.0 35.0 2.0 35.0 116-117 28.360547724729344 34.0 30.0 35.0 2.0 35.0 118-119 28.219454538595983 34.0 29.5 35.0 2.0 35.0 120-121 28.022756897903072 34.0 29.0 35.0 2.0 35.0 122-123 27.806751834760487 34.0 29.0 35.0 2.0 35.0 124-125 25.817718484242647 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4366890979678466 1101 2 0.7713661785862307 1101 3 0.530634076964553 1101 4 0.4091877524482399 1101 5 0.5947881687038858 1101 6 0.5198075969855083 1101 7 0.5962189733053833 1101 8 0.5365453749843354 1101 9 0.5032329753854654 1101 10-11 0.5504304400016835 1101 12-13 0.7225742468435712 1101 14-15 0.7155270652206625 1101 16-17 0.6328142835511414 1101 18-19 0.7094800617281933 1101 20-21 0.48209614207871 1101 22-23 0.5649281192729987 1101 24-25 0.6306119067353393 1101 26-27 0.7199549465693238 1101 28-29 0.6278080445019327 1101 30-31 0.6975528095136241 1101 32-33 0.5998116270628131 1101 34-35 0.5038318396219097 1101 36-37 0.40709961860604693 1101 38-39 0.16905915789069326 1101 40-41 0.25989635785751375 1101 42-43 0.11393957550186684 1101 44-45 0.21406613330574942 1101 46-47 0.25382793005304194 1101 48-49 0.15564089931373104 1101 50-51 -0.18506574221424898 1101 52-53 0.3691642097410863 1101 54-55 0.623513092220513 1101 56-57 0.39024724014618073 1101 58-59 0.16951243618717626 1101 60-61 0.42425976397109366 1101 62-63 0.0678885072092541 1101 64-65 -0.08980319198203546 1101 66-67 0.04675200850999772 1101 68-69 0.1987867926902922 1101 70-71 0.18192285374436068 1101 72-73 0.05977566105688581 1101 74-75 0.26359973013266824 1101 76-77 0.12887591621883487 1101 78-79 0.18262274897272945 1101 80-81 0.3205269728682687 1101 82-83 0.2086741107450507 1101 84-85 0.26048010204760175 1101 86-87 0.421316418494726 1101 88-89 0.4310901490608998 1101 90-91 0.4480012860352929 1101 92-93 0.4205544170532889 1101 94-95 0.2700461984817544 1101 96-97 0.2555316677021331 1101 98-99 0.3566149558023852 1101 100-101 0.24557270751242655 1101 102-103 0.5251144839755426 1101 104-105 0.2036907839785158 1101 106-107 -8.056792048947159E-4 1101 108-109 0.07140152897181551 1101 110-111 0.08449062010948083 1101 112-113 0.06777204135807935 1101 114-115 0.24818012143668966 1101 116-117 0.2577022578118715 1101 118-119 0.2164942200840052 1101 120-121 0.016622788167872216 1101 122-123 0.10398305549366782 1101 124-125 -0.03398450421501664 1102 1 0.0974978154508861 1102 2 0.5750225003639109 1102 3 0.4834945010871934 1102 4 0.1803938845992974 1102 5 0.26629343017496154 1102 6 0.2705153583230455 1102 7 0.47366943001176054 1102 8 0.40101575584005644 1102 9 0.5450443102500557 1102 10-11 0.43419065580211935 1102 12-13 0.5397180959969248 1102 14-15 0.7143051753643448 1102 16-17 0.7923076332653665 1102 18-19 0.5969259780788221 1102 20-21 0.8599939573663562 1102 22-23 0.6222567876133667 1102 24-25 0.4196990281497932 1102 26-27 0.5946678358380524 1102 28-29 0.4215089685978697 1102 30-31 0.4550577504890043 1102 32-33 0.8442029523125356 1102 34-35 0.843083215082622 1102 36-37 0.9379072677700933 1102 38-39 0.8649888625004891 1102 40-41 0.4375511305726434 1102 42-43 0.44148618091823977 1102 44-45 0.5565758817166255 1102 46-47 0.15032117293536373 1102 48-49 -0.061167764753356835 1102 50-51 -0.09476607873473597 1102 52-53 -0.09060690291314444 1102 54-55 0.06029531539234512 1102 56-57 0.045680977660197186 1102 58-59 -0.01681575140963787 1102 60-61 0.3201454004221347 1102 62-63 -0.02164997898272958 1102 64-65 -0.1489880645849908 1102 66-67 -0.09020648729684666 1102 68-69 -0.10888907932508829 1102 70-71 0.03573007558080832 1102 72-73 0.07537979545141482 1102 74-75 -0.026201829613068384 1102 76-77 0.0014588444812133616 1102 78-79 -0.1636461469195858 1102 80-81 -0.014584666467236218 1102 82-83 0.47089968630191237 1102 84-85 0.3346815335950595 1102 86-87 0.34112572355321547 1102 88-89 0.23839878642001366 1102 90-91 0.18662368868472257 1102 92-93 0.06100498059156223 1102 94-95 0.16586506499529818 1102 96-97 0.09902293419430563 1102 98-99 0.04296985137420606 1102 100-101 0.013820931432089623 1102 102-103 0.3552050240107967 1102 104-105 0.20471236402231696 1102 106-107 0.13987190212088763 1102 108-109 0.1874276493713687 1102 110-111 0.3399991399535054 1102 112-113 0.35607127750999723 1102 114-115 0.21491533857252776 1102 116-117 0.45566844551552066 1102 118-119 0.5932502694403432 1102 120-121 0.7175468841029158 1102 122-123 0.7398398473317123 1102 124-125 0.646401158944002 1103 1 -6.302366380985802E-4 1103 2 0.46753470871985314 1103 3 0.4197668830730876 1103 4 0.24159844238551642 1103 5 0.2773352098819615 1103 6 0.09132382278315987 1103 7 0.19002862697323764 1103 8 0.37537820836311653 1103 9 0.24621088648445522 1103 10-11 0.1749123052866537 1103 12-13 0.19190203522644111 1103 14-15 0.07058569625420574 1103 16-17 0.5574188649528296 1103 18-19 0.29898783374891735 1103 20-21 0.26137757103970927 1103 22-23 0.3827424088830398 1103 24-25 0.2240397769289615 1103 26-27 0.3655712541777163 1103 28-29 -0.10342320720245368 1103 30-31 0.039037674525900457 1103 32-33 -0.167218642912637 1103 34-35 -0.13008553152616997 1103 36-37 -0.12279539093854197 1103 38-39 0.2162639574543732 1103 40-41 -0.352709314354108 1103 42-43 -0.019650552668302623 1103 44-45 0.10832574606822476 1103 46-47 0.2695696808029666 1103 48-49 0.09212849135082024 1103 50-51 -0.06561523771465261 1103 52-53 -0.2514316451811851 1103 54-55 -0.21705140191638606 1103 56-57 -0.09508136634414655 1103 58-59 -0.09526122075310184 1103 60-61 -0.17261097505263479 1103 62-63 -0.07363044561321885 1103 64-65 0.0243841546228154 1103 66-67 -0.16793302012375477 1103 68-69 -0.1482271151362724 1103 70-71 -0.3663182152493647 1103 72-73 -0.13670647693056992 1103 74-75 -0.2697449658040618 1103 76-77 -0.19661494824802972 1103 78-79 -0.09354305413608088 1103 80-81 0.2378163102012394 1103 82-83 0.023856821407719053 1103 84-85 0.10950519067591102 1103 86-87 0.23011107352608562 1103 88-89 0.1950184283082379 1103 90-91 0.0052210842354583065 1103 92-93 0.05743742768868998 1103 94-95 -0.14898843473340762 1103 96-97 -0.03347842228968645 1103 98-99 0.08972785462976773 1103 100-101 0.3002018321374642 1103 102-103 0.5442581981833392 1103 104-105 0.07687731247592566 1103 106-107 -0.03961805989755973 1103 108-109 -0.06953111351522878 1103 110-111 0.003143469850414249 1103 112-113 -0.09822877674936592 1103 114-115 0.0882332984206009 1103 116-117 0.06314267231964266 1103 118-119 0.1242730583714362 1103 120-121 -0.07778681095948059 1103 122-123 0.01809540891608208 1103 124-125 0.22793941179261878 1104 1 0.25471431029624725 1104 2 0.41007404675566406 1104 3 0.3443192433552369 1104 4 0.2551361526405387 1104 5 0.27918002811310316 1104 6 0.3813401005910819 1104 7 0.29325705887773523 1104 8 0.3391600857369639 1104 9 0.44195152674490146 1104 10-11 0.168726738276348 1104 12-13 0.19306861146083776 1104 14-15 0.13440826814785112 1104 16-17 0.31034887037876757 1104 18-19 0.4049156688004629 1104 20-21 0.46953003984058483 1104 22-23 0.4624629731803793 1104 24-25 0.30243098691268244 1104 26-27 0.36528639253908324 1104 28-29 0.37769453560817823 1104 30-31 0.36614022471580654 1104 32-33 0.014306045096041942 1104 34-35 0.09952651405168922 1104 36-37 0.1389381956051423 1104 38-39 0.12014350167574861 1104 40-41 0.210747006861304 1104 42-43 0.27009442834091857 1104 44-45 0.08879237656199024 1104 46-47 0.0871768430384563 1104 48-49 0.19012089504045804 1104 50-51 5.947460075361732E-4 1104 52-53 -0.29808112971727496 1104 54-55 -0.08918922069012325 1104 56-57 -0.04967984708207496 1104 58-59 -0.19980544213128582 1104 60-61 -0.21980305318611215 1104 62-63 -0.3863407006322106 1104 64-65 -0.3629055903581886 1104 66-67 -0.2036085491525128 1104 68-69 -0.3949715535518976 1104 70-71 -0.2516410584398123 1104 72-73 -0.31637278186817497 1104 74-75 -0.2581605924996673 1104 76-77 -0.16735558850847454 1104 78-79 0.10310643040000045 1104 80-81 -0.1241207489414613 1104 82-83 -0.18502814874963036 1104 84-85 0.02669184287341153 1104 86-87 0.09499170293466008 1104 88-89 0.029635899822075373 1104 90-91 0.1505412144579168 1104 92-93 0.058427661003932485 1104 94-95 0.08596815777880451 1104 96-97 0.15830128470976845 1104 98-99 0.12802139776595922 1104 100-101 0.07696526132899706 1104 102-103 -0.08938260485518512 1104 104-105 -0.06461753288489902 1104 106-107 0.043222417584807715 1104 108-109 -0.13344863928842798 1104 110-111 0.05649398531433292 1104 112-113 0.013287772355354832 1104 114-115 0.02032770970654596 1104 116-117 0.03427669293819946 1104 118-119 -0.07943014293078932 1104 120-121 -0.28245311589708066 1104 122-123 0.006592424651298501 1104 124-125 0.0381537362635882 1105 1 0.37535244157642467 1105 2 0.695882476874413 1105 3 0.4741639516940097 1105 4 0.4041900479817855 1105 5 0.6668088940924832 1105 6 0.4368601319104073 1105 7 0.47730377687329906 1105 8 0.4526600204883664 1105 9 0.43615092645781317 1105 10-11 0.2676338242740002 1105 12-13 0.33582275474676493 1105 14-15 0.27405005334946964 1105 16-17 0.4333848876043902 1105 18-19 0.1961397324830969 1105 20-21 0.45693053572991715 1105 22-23 0.5221328152787734 1105 24-25 0.416045106709106 1105 26-27 0.37501495837491916 1105 28-29 0.24882529379688378 1105 30-31 0.12756199164781634 1105 32-33 0.18577935841980775 1105 34-35 0.34075057773432604 1105 36-37 0.3255557083286149 1105 38-39 0.2408142572544989 1105 40-41 0.3432766949730137 1105 42-43 0.23519621615248099 1105 44-45 0.510957325015589 1105 46-47 0.4450360365657957 1105 48-49 0.46501323485514234 1105 50-51 0.36914492217874795 1105 52-53 -0.07579873378878688 1105 54-55 0.2668593242661572 1105 56-57 -0.040417808715893955 1105 58-59 0.20728708037947285 1105 60-61 -0.010002713893413784 1105 62-63 0.101219654320154 1105 64-65 0.3447872192464061 1105 66-67 0.29515825236125437 1105 68-69 0.3818195204400112 1105 70-71 0.3622960419125292 1105 72-73 0.3350789994185277 1105 74-75 0.28956549388947295 1105 76-77 0.44857159405709623 1105 78-79 0.493798718015821 1105 80-81 0.22279299241310113 1105 82-83 0.1108155156115842 1105 84-85 0.49922870833080424 1105 86-87 0.6662203340190906 1105 88-89 0.6571936448305564 1105 90-91 0.3894815772401188 1105 92-93 0.37049538904778245 1105 94-95 0.6329569350200934 1105 96-97 0.3080272406017208 1105 98-99 0.37781912711477617 1105 100-101 0.2471205529902285 1105 102-103 0.2398944406883352 1105 104-105 0.4221304770328871 1105 106-107 0.3438096549592018 1105 108-109 0.1785701523075538 1105 110-111 0.40820676098965336 1105 112-113 0.5535200573612293 1105 114-115 0.7017742711054744 1105 116-117 0.7036490014335683 1105 118-119 0.45780070660594774 1105 120-121 0.6174163869086087 1105 122-123 0.5823525708081547 1105 124-125 0.6969100980018155 1106 1 0.3549733245035611 1106 2 0.6558169815661472 1106 3 0.5049593418319276 1106 4 0.3938241559230917 1106 5 0.4687989438437299 1106 6 0.4232824506962274 1106 7 0.4459856837943903 1106 8 0.3681773704474338 1106 9 0.3932389042901647 1106 10-11 0.3369769819035753 1106 12-13 0.04076953980879239 1106 14-15 0.15012222414108578 1106 16-17 0.21258382960326117 1106 18-19 0.25831619195787425 1106 20-21 0.3067101188270911 1106 22-23 0.49017236436876743 1106 24-25 0.3316726651195836 1106 26-27 0.3756982507736879 1106 28-29 0.6132500056804986 1106 30-31 0.7716705627165226 1106 32-33 0.7716789742646313 1106 34-35 0.7402404700449239 1106 36-37 1.0529377852856072 1106 38-39 0.7751929964698192 1106 40-41 1.1216884337474937 1106 42-43 1.241314359612396 1106 44-45 1.0141911558613614 1106 46-47 1.3041140641493953 1106 48-49 1.237794896168829 1106 50-51 0.9512281767917585 1106 52-53 1.0964539269581195 1106 54-55 1.086876327855805 1106 56-57 1.0978749049310608 1106 58-59 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-0.3478994514611742 2216 64-65 -0.2544170149678209 2216 66-67 -0.5173482248021202 2216 68-69 0.07984306030233057 2216 70-71 -0.16778020355345546 2216 72-73 0.022650248215626334 2216 74-75 0.059640999108218296 2216 76-77 0.1453441160794533 2216 78-79 0.12049203358321847 2216 80-81 0.16705607628311014 2216 82-83 0.19154687988725172 2216 84-85 -0.019880774236373355 2216 86-87 0.16695317878924598 2216 88-89 0.2916558552088233 2216 90-91 0.01267722458633358 2216 92-93 0.05977660897523762 2216 94-95 0.06329226702098012 2216 96-97 -0.05014508895635217 2216 98-99 0.06209627568239995 2216 100-101 -0.04321922049411597 2216 102-103 -0.18001080766461186 2216 104-105 0.13871941773908247 2216 106-107 0.2745632266521483 2216 108-109 0.2646794128005574 2216 110-111 0.2394007797919322 2216 112-113 0.24928584313367708 2216 114-115 0.17785318146153628 2216 116-117 0.4336105085769546 2216 118-119 0.33348358468722594 2216 120-121 0.29560500190601857 2216 122-123 0.07423575979327524 2216 124-125 -0.4397506466869139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 13.0 4 29.0 5 84.0 6 239.0 7 481.0 8 686.0 9 1061.0 10 1549.0 11 2120.0 12 2231.0 13 3013.0 14 4253.0 15 3248.0 16 1447.0 17 1217.0 18 1173.0 19 1199.0 20 1299.0 21 1273.0 22 1471.0 23 1574.0 24 1850.0 25 2218.0 26 2758.0 27 3350.0 28 3949.0 29 4869.0 30 5995.0 31 7420.0 32 9218.0 33 11600.0 34 15751.0 35 21616.0 36 35085.0 37 58327.0 38 24876.0 39 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.567762526054445 19.18991121567838 12.220530692869994 21.02179556539718 2 19.471303968782877 22.97275207785839 36.188895455326566 21.367048498032165 3 17.03110395788538 28.94290133158973 33.47961121100479 20.54638349952009 4 11.222321417344617 18.396643572365637 37.862079660668854 32.518955349620896 5 9.61277957415399 42.01800849678959 36.05431906157866 12.314892867477761 6 27.53838222535176 40.07343233285971 17.692497914806758 14.695687526981771 7 23.878920477645472 35.97010734029934 23.897362387724392 16.25360979433079 8 26.48719335085315 33.41632192868849 20.964679550855664 19.131805169602703 9 26.546291289969698 11.563496753804692 20.281071474975583 41.60914048125003 10-11 23.51490232074673 26.250172892906992 30.09405374140251 20.140871044943772 12-13 25.237544375846127 26.949917640106126 29.87107428317553 17.94146370087222 14-15 22.55089338480303 27.544878807311377 28.704413903523662 21.199813904361932 16-17 19.21248852619799 29.030919538784595 30.244942096593697 21.51164983842372 18-19 18.666566074429873 25.96348501804373 35.12429428258874 20.245654624937654 20-21 19.76784150016556 25.4485785059538 36.007200727617175 18.77637926626346 22-23 19.846219618000983 25.425945252675124 35.79008914986986 18.937745979454036 24-25 19.3476593443901 25.9058540490471 35.9212781920222 18.825208414540608 26-27 18.716862192826937 26.137425760833576 36.02187042881632 19.12384161752317 28-29 19.15276188560148 24.755749475034264 36.65664935641925 19.434839282945006 30-31 19.26404204755498 25.538273250428567 36.19958338048595 18.998101321530513 32-33 18.924752815534795 26.223976997908522 35.63794339171874 19.213326794837943 34-35 19.51032434777324 25.48111481580056 35.702774465445444 19.305786370980755 36-37 19.525581863219706 25.477498774032114 35.21859950458323 19.77831985816495 38-39 19.174976004560182 26.037462225519413 35.471966201008435 19.31559556891197 40-41 19.215841600757795 25.541416757828383 35.88292740174444 19.359814239669387 42-43 19.549039209152504 25.90520141085047 34.22297389339822 20.322785486598807 44-45 19.085814169901326 26.101810011653154 34.27012684333632 20.542248975109196 46-47 19.825911262111912 26.10052545204176 33.529583138585615 20.54398014726071 48-49 19.830106702045384 26.93561199103795 32.382635085711655 20.851646221205016 50-51 20.3545387286823 26.433842561833643 32.23083162344617 20.98078708603789 52-53 19.88376528383822 26.840662449857273 31.87379751769928 21.401774748605227 54-55 20.201996914925722 26.88730257201301 31.96677676804936 20.943923745011904 56-57 20.408804833730485 26.83240175459554 31.37146413998202 21.38732927169195 58-59 20.121972943802326 26.186040175624274 32.131068520711295 21.5609183598621 60-61 20.743399998742557 27.19051720705258 31.476509926460572 20.589572867744295 62-63 19.9668037270361 26.87934076896316 32.43217188292446 20.72168362107628 64-65 20.28153901287401 26.36562348715219 32.22630421889375 21.12653328108005 66-67 20.412440271607007 26.982773074021292 31.463240841646407 21.14154581272529 68-69 20.178427723569055 26.844633336686453 31.96232773362841 21.014611206116086 70-71 20.669124257850076 25.79339049084062 32.526966788987366 21.010518462321944 72-73 20.360799896052075 27.19914829764315 31.28287667500765 21.157175131297127 74-75 20.121884771109787 27.007452239444056 31.522176489819188 21.34848649962697 76-77 20.08382756669531 26.555315715573062 32.237567324014506 21.123289393717123 78-79 19.957290212794494 26.801334523674804 31.65532908331133 21.586046180219377 80-81 19.904772283369518 27.224145591107607 31.043732658244828 21.827349467278047 82-83 20.458784426645206 26.68271951112173 31.19574830145817 21.662747760774895 84-85 19.72265765803526 26.901829948110954 30.996998985690695 22.378513408163094 86-87 19.94479977869615 27.383207732222342 30.908561273503054 21.76343121557845 88-89 20.189744492505785 26.58770076786373 31.11377963316903 22.108775106461458 90-91 20.13466983257434 26.648741425957787 31.203750468913398 22.012838272554472 92-93 20.432173925799873 27.186175128727978 30.336002548363368 22.04564839710878 94-95 19.996982188625786 26.420100300314147 30.84999591338043 22.73292159767964 96-97 20.323325635103924 26.57754324490848 30.760154746985325 22.338976373002268 98-99 19.959720516549794 26.6938549687111 30.53549498497378 22.81092952976532 100-101 19.838044801736913 26.734972305409617 30.480814973311343 22.94616791954213 102-103 20.538006450544778 26.348107274361915 30.22346380422869 22.89042247086462 104-105 19.583376993524322 26.89481736068906 30.333843284364065 23.187962361422553 106-107 19.837164951694362 26.266529748307732 30.577991072364146 23.318314227633756 108-109 20.20839620427187 26.630662575864267 29.56945981289609 23.591481406967777 110-111 19.764946517788285 27.089829996982196 29.520001341246687 23.625222143982832 112-113 19.52305299737172 26.530962990287517 30.51090086729069 23.43508314505007 114-115 19.668516162300055 26.665436930242503 29.739009405387502 23.92703750206994 116-117 19.463908795744135 26.87540742386545 29.799217326964072 23.861466453426345 118-119 19.30129027390025 26.029117097177156 30.067825314184628 24.601767314737963 120-121 19.378420124953106 26.48913628942509 29.80179779896424 24.330645786657556 122-123 19.19666341811018 26.78990222892892 29.455395223574044 24.55803912938686 124-125 19.426967846319577 26.32129369207748 29.15103553210129 25.10070292950166 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 98.0 1 83.5 2 117.5 3 205.5 4 198.5 5 203.0 6 346.5 7 841.0 8 1330.0 9 1494.0 10 1262.0 11 849.5 12 714.0 13 679.0 14 678.5 15 649.5 16 651.0 17 687.0 18 678.0 19 666.5 20 706.5 21 810.5 22 886.5 23 903.0 24 1001.0 25 1159.5 26 1378.0 27 1732.5 28 2173.5 29 2631.0 30 3415.5 31 4166.0 32 5077.0 33 6339.5 34 7452.5 35 8217.5 36 9474.5 37 10689.0 38 10288.5 39 9607.0 40 8881.5 41 8325.0 42 7649.5 43 7602.0 44 8139.5 45 8049.0 46 7544.0 47 6962.0 48 6833.0 49 6526.5 50 6452.0 51 6147.0 52 5494.0 53 5328.0 54 5159.0 55 4747.0 56 4300.0 57 3993.0 58 3689.0 59 3074.0 60 2710.5 61 2347.5 62 1827.0 63 1387.0 64 885.5 65 691.0 66 612.0 67 481.5 68 413.0 69 360.0 70 304.0 71 270.5 72 240.0 73 193.5 74 158.0 75 134.0 76 95.0 77 47.5 78 19.0 79 6.0 80 3.0 81 6.5 82 5.5 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06035534207647525 2 0.0 3 0.0 4 0.0 5 0.06035534207647525 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 2.0956715998776126E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.003143507399816419 44-45 0.010478357999388064 46-47 0.01278359675925344 48-49 0.010687925159375826 50-51 0.00984965651942478 52-53 0.007544417759559406 54-55 0.00796355207953493 56-57 0.005658313319669555 58-59 0.003981776039767465 60-61 0.003143507399816419 62-63 0.0025148059198531352 64-65 0.002305238759865374 66-67 0.0029339402398286577 68-69 0.0012574029599265676 70-71 0.0027243730798408965 72-73 0.0025148059198531352 74-75 4.191343199755225E-4 76-77 8.38268639951045E-4 78-79 0.0 80-81 4.191343199755225E-4 82-83 0.0 84-85 4.191343199755225E-4 86-87 4.191343199755225E-4 88-89 0.0012574029599265676 90-91 0.0014669701199143288 92-93 0.0010478357999388064 94-95 0.0014669701199143288 96-97 0.00167653727990209 98-99 0.00167653727990209 100-101 0.0010478357999388064 102-103 0.0010478357999388064 104-105 8.38268639951045E-4 106-107 8.38268639951045E-4 108-109 0.0012574029599265676 110-111 0.0012574029599265676 112-113 0.01173576095931463 114-115 0.022423686118690455 116-117 0.019070611558886275 118-119 0.013831432559192244 120-121 0.007334850599571645 122-123 0.00817311923952269 124-125 0.005867880479657315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 238587.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.43449140145943 #Duplication Level Percentage of deduplicated Percentage of total 1 51.02224318627378 14.50791535163274 2 14.209696201412125 8.080909689128076 3 9.765481051281673 8.330294609513512 4 6.762872009551746 7.691953040190789 5 4.712489497501511 6.6998621048087275 6 3.1072655179021536 5.3012108790504096 7 2.1638831974764523 4.30702427206847 8 1.5772173169617192 3.587789778990473 9 1.1128963311271944 2.848017704233676 >10 5.204817145973674 25.298109285082592 >50 0.26237820786839816 5.090386316102721 >100 0.09286419716690497 4.705621010365191 >500 0.0014740348756651582 0.25148059198531353 >1k 0.004422104626995475 3.2994253668473132 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3064 1.2842275564050012 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2478 1.0386148448993449 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2330 0.9765829655429676 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 600 0.25148059198531353 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 500 0.20956715998776126 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 498 0.20872889134781022 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 481 0.20160360790822635 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 418 0.17519814574976844 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 369 0.15466056407096782 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 278 0.11651934095319527 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 262 0.10981319183358691 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 251 0.10520271431385617 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 245 0.10268790839400303 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.095671599877613E-4 0.0 20-21 0.0 0.0 0.0 0.0010478357999388064 0.0 22-23 0.0 0.0 0.0 0.0016765372799020903 0.0 24-25 0.0 0.0 0.0 0.002305238759865374 0.0 26-27 0.0 0.0 0.0 0.0025148059198531352 0.0 28-29 0.0 0.0 0.0 0.003143507399816419 0.0 30-31 0.0 0.0 0.0 0.00964008935943702 0.0 32-33 0.0 0.0 0.0 0.020747148838788365 0.0 34-35 0.0 0.0 0.0 0.036045551517894944 0.0 36-37 0.0 0.0 0.0 0.0557448645567445 0.0 38-39 0.0 0.0 0.0 0.08990431163474959 0.0 40-41 0.0 0.0 0.0 0.12636899747262004 0.0 42-43 0.0 0.0 0.0 0.17456944426980514 0.0 44-45 0.0 0.0 0.0 0.22486556266686786 0.0 46-47 0.0 0.0 0.0 0.2814486958635634 0.0 48-49 0.0 0.0 0.0 0.34075620214009983 0.0 50-51 0.0 0.0 0.0 0.3977584696567709 0.0 52-53 0.0 0.0 0.0 0.46062861765309926 0.0 54-55 0.0 0.0 0.0 0.5427789443683018 0.0 56-57 0.0 0.0 0.0 0.6307971515631614 0.0 58-59 0.0 0.0 0.0 0.741867746356675 0.0 60-61 0.0 0.0 0.0 0.8508426695503108 0.0 62-63 0.0 0.0 0.0 0.9675715776634939 0.0 64-65 0.0 0.0 0.0 1.1023232615356244 0.0 66-67 0.0 0.0 0.0 1.2594986315264451 0.0 68-69 0.0 0.0 0.0 1.4399359562759078 0.0 70-71 0.0 0.0 0.0 1.641539564184134 0.0 72-73 0.0 0.0 0.0 1.8441910078922992 0.0 74-75 0.0 0.0 0.0 2.0545964365200113 0.0 76-77 0.0 0.0 0.0 2.3031430882654966 0.0 78-79 0.0 0.0 0.0 2.574532560449647 0.0 80-81 0.0 0.0 0.0 2.878614509591889 0.0 82-83 0.0 0.0 0.0 3.180391219974265 0.0 84-85 0.0 0.0 0.0 3.512764735714854 0.0 86-87 0.0 0.0 0.0 3.877621161253547 0.0 88-89 0.0 0.0 0.0 4.260500362551187 0.0 90-91 0.0 0.0 0.0 4.68047295116666 0.0 92-93 0.0 0.0 0.0 5.113438703701375 0.0 94-95 0.0 0.0 0.0 5.566313336434927 0.0 96-97 0.0 0.0 0.0 6.105948773403412 0.0 98-99 0.0 0.0 0.0 6.66318785181087 0.0 100-101 0.0 0.0 0.0 7.250814168416553 0.0 102-103 0.0 0.0 0.0 7.823142082343129 0.0 104-105 0.0 0.0 0.0 8.447023517626693 0.0 106-107 0.0 0.0 0.0 9.10192089258845 0.0 108-109 0.0 0.0 0.0 9.795169057827962 0.0 110-111 0.0 0.0 0.0 10.532426326664906 0.0 112-113 0.0 0.0 0.0 11.301747370979978 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1275 0.0 78.52963 1 CGTAAGG 30 0.0042048343 59.598385 1 CGTTATA 15 0.0042337193 59.50467 106-107 CGTGAGG 15 0.0042354777 59.49843 62-63 CAACGCG 15 0.0042389953 59.48596 34-35 GTGGACG 35 0.0077959965 50.987965 9 GTATCAA 1980 0.0 50.525887 2 AGGTCCG 30 2.1917816E-5 49.582024 60-61 TGTCGTG 25 5.33014E-4 47.598743 50-51 CTAAGCG 25 5.335653E-4 47.588768 20-21 GGGATGA 230 0.0 46.554226 7 GATGACC 170 0.0 45.48926 9 ATGGGAT 440 0.0 44.66127 5 TAGGACG 55 8.480789E-4 43.3079 4 GGATGAC 230 0.0 41.381535 8 GTACATG 1870 0.0 41.113323 1 GTCCAAT 60 0.0012958564 39.732258 1 GGCTTAT 135 1.2951205E-9 39.732254 1 ATGGGGG 390 0.0 39.69891 5 GTAGGAC 75 8.130096E-5 39.69891 3 >>END_MODULE