FastQCFastQC Report
Thu 26 May 2016
SRR936184_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936184_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3074863
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37000.068.336121
GTACATG218100.054.3644141
TACATGG221300.052.0997282
ACATGGG218800.050.547793
GTATCAA53250.047.705822
CATGGGA139700.046.6749954
ATCAACG61600.041.143294
TCAACGC62650.040.3594365
CAACGCA63150.039.9456676
CATGGGG76550.039.2478944
AACGCAG65800.038.24657
ATGGGAT65650.037.8807685
ATGGGAG54050.035.333495
GAGTACA126950.033.3664781
AGTACAT124450.032.9372562
TATCAAC78900.032.7252433
ACGCAGA80850.030.6855038
CGCAGAG81500.030.1487739
ATGGGGG42200.028.901385
CATGGGT28300.026.488314
Produced by FastQC (version 0.11.5)