FastQCFastQC Report
Thu 26 May 2016
SRR936184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3074863
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97440.31689216722826347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69350.22553850366666744No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67350.21903414883850109No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66100.085.983811
GTATCAA104850.054.1089442
GTACATG193000.045.129431
TACATGG194300.044.273072
ACATGGG194550.043.634793
TCAACGC148400.039.8345535
ATCAACG148600.039.7408754
CAACGCA151200.039.190126
CATGGGA126000.038.6952744
AACGCAG155400.038.1692127
TATCAAC160050.037.3441543
CATGGGG68700.036.307964
ATGGGAG47550.032.926825
ACGCAGA182300.032.4064678
CGCAGAG185050.031.7962769
GAGTACA127350.029.893151
ATGGGAT58600.029.5624185
AGTACAT124550.029.2995742
CAGAGTA206350.027.1879399
GCAGAGT208500.026.679318
Produced by FastQC (version 0.11.5)