##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936184_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3074863 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.392748880194013 31.0 31.0 34.0 30.0 34.0 2 31.379967172521184 31.0 31.0 34.0 30.0 34.0 3 31.72122075032286 31.0 31.0 34.0 30.0 34.0 4 35.550111338293775 37.0 35.0 37.0 33.0 37.0 5 35.1909532229566 37.0 35.0 37.0 33.0 37.0 6 35.38325805084649 37.0 35.0 37.0 33.0 37.0 7 35.21226409111561 37.0 35.0 37.0 32.0 37.0 8 35.29343746371789 37.0 35.0 37.0 32.0 37.0 9 36.95872303904272 39.0 37.0 39.0 33.0 39.0 10-11 36.84120105513644 39.0 37.0 39.0 32.0 39.0 12-13 36.794489868329094 39.0 37.0 39.0 32.0 39.0 14-15 37.923911569393496 40.0 37.0 41.0 32.5 41.0 16-17 37.831950236482086 40.0 37.0 41.0 32.0 41.0 18-19 37.93030209801217 40.0 37.0 41.0 32.0 41.0 20-21 37.9832161628014 40.0 37.0 41.0 32.5 41.0 22-23 37.89716159711831 40.0 37.0 41.0 32.0 41.0 24-25 37.86751035737202 40.0 37.0 41.0 32.0 41.0 26-27 37.69415808118931 40.0 37.0 41.0 32.0 41.0 28-29 37.54373446882023 40.0 37.0 41.0 32.0 41.0 30-31 37.38510756414188 39.5 36.0 41.0 31.5 41.0 32-33 37.19205684936207 39.0 36.0 41.0 31.0 41.0 34-35 37.00139388323967 39.0 36.0 41.0 31.0 41.0 36-37 36.80642259508798 39.0 35.0 41.0 30.0 41.0 38-39 36.795561298178164 39.0 35.5 41.0 30.5 41.0 40-41 36.833887883785394 39.0 35.0 41.0 30.5 41.0 42-43 36.73449776461585 39.0 35.0 41.0 30.0 41.0 44-45 36.576040298380775 39.5 35.0 41.0 30.0 41.0 46-47 36.31869241003582 39.0 35.0 41.0 30.0 41.0 48-49 36.0600777985881 39.0 35.0 41.0 29.0 41.0 50-51 35.87438172042137 39.0 35.0 41.0 28.0 41.0 52-53 35.73351316790374 39.0 35.0 41.0 28.0 41.0 54-55 35.557045305758336 39.0 35.0 41.0 28.0 41.0 56-57 35.36190669307868 39.0 35.0 40.5 27.0 41.0 58-59 35.15603735841239 38.0 34.0 40.0 26.5 41.0 60-61 34.93628236445006 38.0 34.0 40.0 26.0 41.0 62-63 34.721990540716774 38.0 34.0 40.0 26.0 41.0 64-65 34.47936200734797 37.0 34.0 40.0 26.0 41.0 66-67 34.201098878226446 37.0 34.0 40.0 26.0 41.0 68-69 33.89859954736195 36.0 33.0 39.0 26.0 41.0 70-71 33.58048748838566 36.0 33.0 39.0 25.5 41.0 72-73 33.312596040864264 35.0 33.0 39.0 25.5 40.0 74-75 33.13591499848936 35.0 33.0 37.5 26.0 40.0 76-77 32.83159428566411 35.0 33.0 37.0 25.5 39.0 78-79 32.49958420911761 35.0 33.0 37.0 25.0 39.0 80-81 32.21656493313686 35.0 32.5 36.0 25.0 39.0 82-83 31.959974314302784 35.0 32.0 36.0 25.0 37.0 84-85 31.705338579312315 35.0 32.0 35.5 24.0 37.0 86-87 31.503919686828326 35.0 32.0 35.0 24.0 36.5 88-89 31.343695637821913 35.0 32.0 35.0 24.0 36.0 90-91 31.20605161921035 35.0 32.0 35.0 23.5 36.0 92-93 31.075377829841525 35.0 32.0 35.0 23.0 36.0 94-95 30.953665740554946 35.0 32.0 35.0 23.0 35.5 96-97 30.89816830863684 35.0 32.0 35.0 23.0 35.0 98-99 30.81585911307268 34.0 32.0 35.0 20.5 35.0 100-101 30.73318681190024 34.0 32.0 35.0 20.0 35.0 102-103 29.704832377897812 33.5 29.5 34.5 19.0 35.0 104-105 30.469940449379372 34.0 31.0 35.0 20.0 35.0 106-107 30.628291244195267 34.0 32.0 35.0 20.0 35.0 108-109 30.595537427195943 34.0 32.0 35.0 19.0 35.0 110-111 30.558910266896444 34.0 32.0 35.0 19.0 35.0 112-113 30.475952261938176 34.0 32.0 35.0 19.0 35.0 114-115 30.460870939615845 34.0 32.0 35.0 18.5 35.0 116-117 30.35913469965979 34.0 32.0 35.0 17.5 35.0 118-119 30.226746199749385 34.0 32.0 35.0 12.5 35.0 120-121 30.03027825955173 34.0 31.5 35.0 2.0 35.0 122-123 29.798728593761737 34.0 31.0 35.0 2.0 35.0 124-125 27.703385484166287 32.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15814706903543296 1101 2 0.6211807128549616 1101 3 0.4222068213462009 1101 4 0.2796602660706853 1101 5 0.5079129527724646 1101 6 0.3579963655629257 1101 7 0.46875070153396337 1101 8 0.48967819491468134 1101 9 0.3887165986232901 1101 10-11 0.40150884530866904 1101 12-13 0.4114557667337877 1101 14-15 0.48904307504053435 1101 16-17 0.5799967750188202 1101 18-19 0.5551294402491891 1101 20-21 0.3967759926003609 1101 22-23 0.4335188920398849 1101 24-25 0.4170264352968118 1101 26-27 0.40946559402333094 1101 28-29 0.4548354776887962 1101 30-31 0.4071644696567702 1101 32-33 0.3628350193936001 1101 34-35 0.4176785194000061 1101 36-37 0.432546165055264 1101 38-39 0.30518216014579735 1101 40-41 0.2863901280251113 1101 42-43 0.3209391079713484 1101 44-45 0.3188955633339958 1101 46-47 0.2895093790307257 1101 48-49 0.3262961798047641 1101 50-51 0.34726522367294876 1101 52-53 0.3182823893928983 1101 54-55 0.29016132753348245 1101 56-57 0.0720065480223866 1101 58-59 0.22395895952000444 1101 60-61 0.25751160686903773 1101 62-63 0.19991208158283058 1101 64-65 0.1142223714206736 1101 66-67 0.28528196757925883 1101 68-69 0.22366955078433648 1101 70-71 0.21483817755189705 1101 72-73 0.2908671920891308 1101 74-75 0.25201096949692925 1101 76-77 0.2608447574946169 1101 78-79 0.2793258030102592 1101 80-81 0.23461094000101923 1101 82-83 0.1458374028298941 1101 84-85 0.12270707048260832 1101 86-87 0.18663104324866353 1101 88-89 0.10340909678957644 1101 90-91 0.1912974908176146 1101 92-93 0.22039277547679603 1101 94-95 0.25891925984002384 1101 96-97 0.22770052379696537 1101 98-99 0.23168499112033203 1101 100-101 0.2889775786498703 1101 102-103 0.281646837304244 1101 104-105 0.22263545177106892 1101 106-107 0.22830864991303912 1101 108-109 0.18613001929159267 1101 110-111 0.24825647112558968 1101 112-113 0.30884588302697935 1101 114-115 0.296090075261052 1101 116-117 0.3430491894552148 1101 118-119 0.4289661881082196 1101 120-121 0.37077000953145145 1101 122-123 0.40981629489606064 1101 124-125 0.4330072071184041 1102 1 0.23382787371217972 1102 2 0.6569228130843285 1102 3 0.3830139112278985 1102 4 0.211446238906845 1102 5 0.42793999479127365 1102 6 0.29852170885382634 1102 7 0.47188812901778476 1102 8 0.382105615968122 1102 9 0.4167210826884471 1102 10-11 0.45116810006299346 1102 12-13 0.43019756892440597 1102 14-15 0.46916659838868213 1102 16-17 0.5493470226327659 1102 18-19 0.48267893862811206 1102 20-21 0.4154183516724217 1102 22-23 0.43671600864278304 1102 24-25 0.35707438187488094 1102 26-27 0.4325188453192865 1102 28-29 0.39015508124609966 1102 30-31 0.30973009049102984 1102 32-33 0.2857483528850224 1102 34-35 0.3145965150359018 1102 36-37 0.3555480732472347 1102 38-39 0.2779796723358672 1102 40-41 0.11006515744539769 1102 42-43 0.2309031573369822 1102 44-45 0.26660952238133007 1102 46-47 0.27055340507400416 1102 48-49 0.16377218210538302 1102 50-51 0.134472119294756 1102 52-53 0.20863289645399874 1102 54-55 0.21252031358795875 1102 56-57 0.2282386035087356 1102 58-59 0.21446054340303533 1102 60-61 0.2519240195090404 1102 62-63 0.1810740909870887 1102 64-65 0.12589348309593618 1102 66-67 0.19127608026766296 1102 68-69 0.23642703929868247 1102 70-71 0.1892590361731834 1102 72-73 0.2288905906887635 1102 74-75 0.204033064816322 1102 76-77 0.16179627295892374 1102 78-79 0.13013520373847598 1102 80-81 0.1672619110447613 1102 82-83 0.0958359963287414 1102 84-85 0.1698625207094402 1102 86-87 0.11630156861055241 1102 88-89 0.16488168257103553 1102 90-91 0.13473086241707932 1102 92-93 0.21894053397891966 1102 94-95 0.13337020131237054 1102 96-97 0.15903002307790715 1102 98-99 0.18760233906825974 1102 100-101 0.21930578859934258 1102 102-103 0.32815366964512194 1102 104-105 0.24843277518775508 1102 106-107 0.1631841013301667 1102 108-109 0.11767560829475698 1102 110-111 0.17266133515528637 1102 112-113 0.10189586101116888 1102 114-115 0.09991925720743566 1102 116-117 0.1477899236348641 1102 118-119 0.1099129098446383 1102 120-121 0.07949059564024097 1102 122-123 0.13959261006762702 1102 124-125 0.28303257063501874 1103 1 0.11314278007200329 1103 2 0.5913001483434357 1103 3 0.3105408643980567 1103 4 0.23933752569892164 1103 5 0.43062436990351216 1103 6 0.27112410688019395 1103 7 0.3336060297752468 1103 8 0.24304782528101043 1103 9 0.32995473441673084 1103 10-11 0.3880581466957054 1103 12-13 0.3868796997627726 1103 14-15 0.43943805344009945 1103 16-17 0.5309602945436254 1103 18-19 0.4278774986620917 1103 20-21 0.33880666705930906 1103 22-23 0.3504524105130784 1103 24-25 0.3166760088290772 1103 26-27 0.28619326347275376 1103 28-29 0.2906948548624584 1103 30-31 0.17657798279175552 1103 32-33 0.22547907928535693 1103 34-35 0.20120340599830655 1103 36-37 0.2002513432653501 1103 38-39 0.1469952740464251 1103 40-41 0.24031884446262097 1103 42-43 0.17212492737085228 1103 44-45 0.15327201784433697 1103 46-47 0.22102313318705313 1103 48-49 0.19107393416104657 1103 50-51 0.1085041823626085 1103 52-53 0.0397708624794717 1103 54-55 0.1110445828449329 1103 56-57 0.032284474738879965 1103 58-59 0.1840046361552652 1103 60-61 0.13539575204018917 1103 62-63 0.0675432698685583 1103 64-65 0.16351238174342342 1103 66-67 0.10493164485774997 1103 68-69 0.09346561635006623 1103 70-71 0.13766949231370518 1103 72-73 0.20148403887340294 1103 74-75 0.2109435551972112 1103 76-77 0.14463353677404456 1103 78-79 0.16003402664374278 1103 80-81 0.1410792933927354 1103 82-83 0.11207372306432362 1103 84-85 0.09582665828940762 1103 86-87 0.13937605057927982 1103 88-89 0.1471493322652222 1103 90-91 0.09839421625651923 1103 92-93 0.05518828222760774 1103 94-95 0.12588865328769216 1103 96-97 0.16010325345456877 1103 98-99 0.16495600598009474 1103 100-101 0.10234660068604029 1103 102-103 0.13700808874785153 1103 104-105 0.05915615220902026 1103 106-107 0.029947922859470566 1103 108-109 0.17240804786802144 1103 110-111 0.15332702091930273 1103 112-113 0.15230979985268434 1103 114-115 0.15385735502077935 1103 116-117 0.14531907442422565 1103 118-119 0.13654117187429193 1103 120-121 0.1321581623722814 1103 122-123 0.1117913991020778 1103 124-125 0.06436172260229256 1104 1 0.2085784647193094 1104 2 0.4567417606730153 1104 3 0.250671273408134 1104 4 0.161092119652686 1104 5 0.35178619562964997 1104 6 0.21224246293630955 1104 7 0.30930253244270034 1104 8 0.3216884101456614 1104 9 0.36473047043728 1104 10-11 0.3179139064269876 1104 12-13 0.26378149782640037 1104 14-15 0.30794230161239966 1104 16-17 0.39294409221206905 1104 18-19 0.3816415769074979 1104 20-21 0.33742354119485896 1104 22-23 0.36122103331489797 1104 24-25 0.2880255444939621 1104 26-27 0.22098875843422405 1104 28-29 0.23853125083163462 1104 30-31 0.18843334734421546 1104 32-33 0.16422636243098054 1104 34-35 0.09114610506963317 1104 36-37 0.19926339621930822 1104 38-39 0.15914702271270187 1104 40-41 0.1378687357884445 1104 42-43 0.13507691314441672 1104 44-45 0.12807936817036136 1104 46-47 0.017901220521572725 1104 48-49 0.06165287113002904 1104 50-51 0.034902127432758334 1104 52-53 -0.04222874234170604 1104 54-55 -0.02647764596360247 1104 56-57 0.005906288609658361 1104 58-59 -0.022970269989762926 1104 60-61 0.017731258857018872 1104 62-63 0.06616014400410819 1104 64-65 -0.06006003477504862 1104 66-67 -0.05590613423728996 1104 68-69 -0.007996545278068368 1104 70-71 -0.07315840731087064 1104 72-73 -0.037796735677090965 1104 74-75 -0.002749390860877554 1104 76-77 0.03092083177502758 1104 78-79 0.06706820961153426 1104 80-81 0.03299788655614577 1104 82-83 -0.08709640141700348 1104 84-85 -0.06352920023657305 1104 86-87 0.0024465899983638906 1104 88-89 -0.0026234399141884523 1104 90-91 -0.09731809356367194 1104 92-93 -0.01979689856669964 1104 94-95 0.02531564400099029 1104 96-97 -0.014862168398817488 1104 98-99 -0.01929611810605536 1104 100-101 -0.0654068077312644 1104 102-103 -0.07905593022073631 1104 104-105 -0.09555635520058559 1104 106-107 -0.04651917850389964 1104 108-109 -0.017376579675943304 1104 110-111 -0.03833470601213662 1104 112-113 -0.04518081465038293 1104 114-115 0.007739986911705188 1104 116-117 0.027753375945660963 1104 118-119 0.059876481101717616 1104 120-121 -0.030655510814835196 1104 122-123 0.05775069568377944 1104 124-125 0.04578831813677198 1105 1 0.19054985679372294 1105 2 0.5947272919496314 1105 3 0.2879342547394792 1105 4 0.18526857870867985 1105 5 0.333937915012001 1105 6 0.2552860518997804 1105 7 0.3344747219028932 1105 8 0.24974987463151166 1105 9 0.3147059412996214 1105 10-11 0.18164701384257143 1105 12-13 0.1885946625521555 1105 14-15 0.18998914987636795 1105 16-17 0.36082654639583467 1105 18-19 0.28269640688967 1105 20-21 0.21541710936674718 1105 22-23 0.25976430462488054 1105 24-25 0.21306984027883402 1105 26-27 0.24970913813263707 1105 28-29 0.20432391188010257 1105 30-31 0.1361490049331735 1105 32-33 0.1305787124348754 1105 34-35 0.141033038306297 1105 36-37 0.20582058648558643 1105 38-39 0.09802251934489448 1105 40-41 0.11573953111818014 1105 42-43 0.0972689618814897 1105 44-45 0.15995784544341518 1105 46-47 0.1214310389520108 1105 48-49 -0.017100610959467133 1105 50-51 0.07205034276267241 1105 52-53 0.02417703926003867 1105 54-55 0.02731749635779579 1105 56-57 0.0761971989419763 1105 58-59 0.09750004674821611 1105 60-61 -0.041097539167452624 1105 62-63 0.042606642587223575 1105 64-65 0.05090200447712334 1105 66-67 0.026756000048912654 1105 68-69 0.09477110129027011 1105 70-71 0.03593287098163955 1105 72-73 0.014698836285688799 1105 74-75 0.04736967525327174 1105 76-77 0.07499937618533892 1105 78-79 0.11084457092087519 1105 80-81 0.15406759893562594 1105 82-83 0.145989282413435 1105 84-85 0.1742028594311087 1105 86-87 0.16622464823391425 1105 88-89 0.12879517189845302 1105 90-91 0.09985155374385357 1105 92-93 0.16129067392256857 1105 94-95 0.13411550874009848 1105 96-97 0.15936223537282856 1105 98-99 0.1309307282529204 1105 100-101 0.10691452643804666 1105 102-103 0.17916922984672468 1105 104-105 0.15686538660091998 1105 106-107 0.1382040314084918 1105 108-109 0.11015563186741062 1105 110-111 0.1579483898285936 1105 112-113 0.10867976182365524 1105 114-115 0.15504932623252543 1105 116-117 0.12691725062157744 1105 118-119 0.12640685423128772 1105 120-121 0.2500161220967172 1105 122-123 0.23506921551408055 1105 124-125 0.3807925111319932 1106 1 0.24461479324277136 1106 2 0.5323603214168635 1106 3 0.3287080352784528 1106 4 0.16596831906578302 1106 5 0.39379535642390806 1106 6 0.17006526968804536 1106 7 0.32251309502792225 1106 8 0.2290985715475813 1106 9 0.15004608635418748 1106 10-11 0.16316020546563692 1106 12-13 0.15463225870853847 1106 14-15 0.16727196615957496 1106 16-17 0.19290465578639981 1106 18-19 0.17820675616111714 1106 20-21 0.19393627663519908 1106 22-23 0.11681317432996252 1106 24-25 0.08874865423940292 1106 26-27 0.21585300473535796 1106 28-29 0.11915990166833268 1106 30-31 0.18749497219942413 1106 32-33 0.15488118630062075 1106 34-35 0.24862451511024375 1106 36-37 0.13949783640243396 1106 38-39 0.14613864599231619 1106 40-41 0.141153863356962 1106 42-43 0.31381363956965913 1106 44-45 0.2776820714913768 1106 46-47 0.26217962237002723 1106 48-49 0.3131003473733287 1106 50-51 0.2959815055451145 1106 52-53 0.2722294495778925 1106 54-55 0.33432707356067226 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-0.5361432307102412 2216 86-87 -0.5829969440382001 2216 88-89 -0.5653659393060337 2216 90-91 -0.4584762152141124 2216 92-93 -0.47651872190086664 2216 94-95 -0.5583432473966319 2216 96-97 -0.6646203052216464 2216 98-99 -0.6990288682700196 2216 100-101 -0.9095712725629639 2216 102-103 -0.9051629534308141 2216 104-105 -0.6298184264910738 2216 106-107 -0.7063844925830054 2216 108-109 -0.7311979782025553 2216 110-111 -0.8211650523780492 2216 112-113 -0.6575582187435565 2216 114-115 -0.7828115623460157 2216 116-117 -0.7783504047467353 2216 118-119 -0.8224615657356793 2216 120-121 -0.7876891422353047 2216 122-123 -0.8236967957532073 2216 124-125 -0.9611490827903317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 94.0 3 114.0 4 281.0 5 586.0 6 1576.0 7 3177.0 8 5099.0 9 8226.0 10 13395.0 11 16805.0 12 18183.0 13 21882.0 14 29039.0 15 22104.0 16 11458.0 17 10054.0 18 9832.0 19 9226.0 20 9501.0 21 10125.0 22 11437.0 23 14060.0 24 17934.0 25 23291.0 26 30483.0 27 39054.0 28 50455.0 29 63481.0 30 79918.0 31 100155.0 32 125789.0 33 161384.0 34 217145.0 35 306194.0 36 505841.0 37 803850.0 38 322300.0 39 1335.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.2402474837675 18.63271466730538 10.857126678573103 23.269911170354014 2 18.993273508329008 21.293803256541686 36.590449572126445 23.122473663002864 3 17.869058626140987 26.740300443240123 32.01410667227777 23.37653425834112 4 11.653299675465217 16.763088306698542 37.572600795547636 34.0110112222886 5 10.818807365572765 41.29904700539711 35.69520443743705 12.186941191593082 6 30.867704263704926 37.81663626420836 17.899573346567536 13.41608612551917 7 26.691693255927174 34.123016212429626 22.618601218981137 16.56668931266206 8 26.91817489104393 33.603383305207416 20.307246209018093 19.171195594730563 9 26.789323621897953 11.763385880931931 19.754636222817084 41.69265427435304 10-11 24.248755147790323 25.51138050703397 29.968457131260806 20.271407213914898 12-13 25.464614195819458 24.773233799359517 29.599204907665804 20.16294709715522 14-15 22.633089669360878 26.21869657282292 27.33551055770615 23.812703200110054 16-17 21.68842754192791 28.466181072900532 27.613849861831152 22.231541523340404 18-19 20.922200436247078 26.79197414649043 30.608957212077414 21.676868205185077 20-21 22.186809623713316 26.089357477064834 30.661723790620915 21.062109108600936 22-23 21.67628281325054 26.540141788430898 30.331985522607024 21.451589875711534 24-25 21.543272260141713 26.517819902515967 30.553593859888046 21.385313977454278 26-27 21.477021903089664 26.53866204770749 30.493082130813633 21.49123391838921 28-29 21.257190970784716 26.532678041265576 30.662162834571816 21.54796815337789 30-31 21.532601615096347 26.293025087621793 30.770330255364225 21.40404304191764 32-33 21.22335206479118 26.61003108105955 30.50287118483002 21.66374566931925 34-35 21.722043749662586 26.613196446795268 30.307367039388485 21.357392764153662 36-37 21.871998850030067 26.341482531091632 29.938992403889213 21.84752621498909 38-39 21.585563324284692 26.57227330128204 30.098804402017258 21.743358972416008 40-41 21.769233583615527 26.610669582786223 30.08152397058405 21.5385728630142 42-43 21.701038086141082 26.583952155478435 29.83623613036715 21.87877362801333 44-45 21.695325893610732 26.869045725639655 29.520255957160934 21.915372423588675 46-47 22.013203960797938 26.934050450605163 28.794675092014003 22.258070496582892 48-49 22.098630354411366 27.06415259785773 28.63959791725143 22.197619130479467 50-51 22.106890915480463 27.102174073190493 28.497582059314315 22.29335295201473 52-53 22.18603937149494 27.181793374944423 28.49969476309746 22.132472490463176 54-55 22.020875495888568 27.155479919308085 28.638604462448257 22.18504012235509 56-57 22.10663924287972 27.35960188675134 28.359714743180074 22.174044127188868 58-59 22.272659787752435 26.924878815685847 28.444998200671694 22.357463195890016 60-61 22.278301768668456 27.065964311813552 28.694753705851994 21.960980213665998 62-63 21.933036839494928 27.070581252591612 28.840486856975602 22.155895050937858 64-65 21.90235943187347 26.996205941964508 28.81070948169772 22.290725144464304 66-67 21.97008896556995 27.43447102805562 28.38700917299909 22.208430833375342 68-69 22.08111959342149 27.133511912282643 28.539556895900443 22.245811598395424 70-71 22.00291174498669 26.853261614579342 28.679614874323878 22.464211766110093 72-73 22.1225316001204 27.136157731721884 28.364664194194695 22.37664647396302 74-75 21.804679267864344 27.36986314735076 28.416080186286113 22.409377398498783 76-77 21.77400458006752 27.316299306745655 28.413297513647436 22.49639859953939 78-79 22.046622595297805 27.228499109390636 28.2198772562402 22.50500103907136 80-81 21.793601773353643 27.332274854204364 28.096815716658885 22.77730765578311 82-83 22.043543778179135 27.275262495733543 28.157611754293164 22.523581971794158 84-85 21.871125326051907 27.13690122999276 28.17392557146181 22.818047872493523 86-87 21.80616793813687 27.185661751254734 28.17707516051583 22.831095150092565 88-89 21.944011493327675 27.107455621494214 28.142489294114874 22.806043591063236 90-91 21.955107513855314 27.196889697031985 28.151175372290933 22.696827416821773 92-93 21.984548517074245 27.059201740052075 28.102276842402578 22.853972900471096 94-95 21.711949694738976 27.025906331330784 28.224656505121022 23.037487468809225 96-97 21.84771231212509 27.15277340760113 28.10982176671566 22.889692513558117 98-99 21.750885091884996 27.112089740479284 27.96761893859222 23.169406229043503 100-101 21.664380638001195 27.06011293279726 28.070995621719845 23.204510807481697 102-103 21.7879664938609 26.87252456591637 28.065806361600337 23.273702578622395 104-105 21.556963540264157 26.776382801859217 28.211707650859687 23.454946007016943 106-107 21.571440279324378 26.91302139162651 28.15200946518332 23.36352886386579 108-109 21.738481409385095 26.9167757914262 27.742557387861407 23.602185411327305 110-111 21.7135016543961 26.954363124171092 27.757787564743246 23.574347656689557 112-113 21.564841115190877 26.890984390098886 27.96820840175858 23.575966092951656 114-115 21.371906012152238 26.9955730644416 27.846707902760627 23.785813020645534 116-117 21.30705696523692 27.03409170749986 27.909804390989024 23.74904693627419 118-119 21.256623398081715 26.94554949606719 27.69734237930706 24.10048472654404 120-121 21.175282131490665 27.0491396731589 27.833238933096823 23.942339262253615 122-123 21.239096641979575 26.960400964847274 27.675450172351074 24.125052220822077 124-125 21.209953475211442 26.939641363968253 27.415914697507564 24.43449046331274 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 55.0 1 75.0 2 343.0 3 645.0 4 554.5 5 513.5 6 789.5 7 1807.0 8 2827.0 9 3347.5 10 3199.0 11 2598.5 12 2540.0 13 2758.0 14 3077.5 15 3321.0 16 3696.0 17 4073.0 18 4513.5 19 5110.5 20 5723.0 21 6660.5 22 8291.0 23 10516.0 24 13302.0 25 16702.5 26 21782.5 27 27494.5 28 31281.0 29 35373.0 30 41686.0 31 48104.5 32 54603.0 33 61505.0 34 70112.5 35 77529.5 36 83835.5 37 90872.5 38 94223.0 39 94739.0 40 94949.0 41 96897.0 42 98981.0 43 102093.5 44 107983.5 45 113003.0 46 113023.5 47 111930.0 48 112513.0 49 110976.5 50 108296.0 51 102817.0 52 96573.0 53 93233.5 54 89609.0 55 83642.5 56 76093.5 57 68705.5 58 62228.0 59 55998.5 60 48233.5 61 39500.5 62 32196.0 63 25241.5 64 19686.0 65 16313.0 66 12586.5 67 9615.0 68 7353.0 69 5007.0 70 3826.0 71 3170.5 72 2872.5 73 2802.5 74 2157.0 75 1604.0 76 1152.0 77 765.0 78 527.0 79 259.5 80 119.5 81 73.0 82 43.0 83 19.5 84 14.5 85 12.5 86 6.0 87 4.0 88 1.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06553137489377575 2 1.9513064484498986E-4 3 1.9513064484498986E-4 4 0.0 5 0.062376762802115085 6 6.504354828166328E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.504354828166328E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.626088707041582E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 1.626088707041582E-4 36-37 0.0 38-39 0.0 40-41 1.626088707041582E-5 42-43 0.002276524189858215 44-45 0.00930122740427785 46-47 0.010764707240615273 48-49 0.008845922566306206 50-51 0.007886530229151673 52-53 0.0065693983764479906 54-55 0.0066832245859409015 56-57 0.005203483862533062 58-59 0.003398525397716906 60-61 0.002081393545013225 62-63 0.0012358274173516022 64-65 0.0017886975777457403 66-67 0.0025366983829848678 68-69 0.0010569576595770283 70-71 0.0024228721734919573 72-73 0.002357828625210294 74-75 7.480008052391277E-4 76-77 5.691310474645537E-4 78-79 6.504354828166328E-5 80-81 3.5773951554914806E-4 82-83 1.1382620949291073E-4 84-85 2.4391330605623727E-4 86-87 3.4147862847873226E-4 88-89 0.0011545229819995232 90-91 0.0016260887070415821 92-93 4.0652217676039553E-4 94-95 0.0011545229819995232 96-97 0.001447218949267008 98-99 0.0013333927397740973 100-101 8.293052405912068E-4 102-103 7.642616923095436E-4 104-105 6.179137086758011E-4 106-107 4.8782661211247455E-4 108-109 6.016528216053853E-4 110-111 8.130443535207911E-4 112-113 0.011057403207882758 114-115 0.019984630209541044 116-117 0.01843984593785154 118-119 0.011252533852727746 120-121 0.006130354425546765 122-123 0.00583765845827928 124-125 0.0051384403142513985 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3074863.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.66170702584055 #Duplication Level Percentage of deduplicated Percentage of total 1 41.80052282950529 11.980743388662399 2 18.945560266820706 10.860241956160412 3 12.98728757632294 11.167134947187204 4 8.592307209099225 9.850807676128785 5 5.56345833058583 7.97291063608615 6 3.4500796293384415 5.9331102931151385 7 2.1760326730200528 4.365816766693021 8 1.4291529038531352 3.276956946029426 9 0.968078088152068 2.497209348667537 >10 3.701419036955226 17.852778775331632 >50 0.2335698932127727 4.676671486141147 >100 0.14623601354925828 7.519184944727801 >500 0.005150904205990207 0.9702159166685591 >1k 8.012517652951957E-4 0.30806233580611314 >5k 3.433936136979411E-4 0.7681545825947106 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9744 0.31689216722826347 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6935 0.22553850366666744 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6735 0.21903414883850109 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.252177414083164E-5 0.0 5 0.0 0.0 0.0 3.252177414083164E-5 0.0 6 0.0 0.0 0.0 3.252177414083164E-5 0.0 7 0.0 0.0 0.0 3.252177414083164E-5 0.0 8 0.0 0.0 0.0 3.252177414083164E-5 0.0 9 0.0 0.0 0.0 3.252177414083164E-5 0.0 10-11 0.0 0.0 0.0 6.504354828166328E-5 0.0 12-13 0.0 0.0 0.0 6.504354828166328E-5 0.0 14-15 0.0 0.0 0.0 6.504354828166328E-5 0.0 16-17 0.0 0.0 0.0 6.504354828166328E-5 0.0 18-19 0.0 0.0 0.0 6.504354828166328E-5 3.252177414083164E-5 20-21 0.0 0.0 0.0 6.504354828166328E-5 3.252177414083164E-5 22-23 0.0 0.0 0.0 6.504354828166328E-5 3.252177414083164E-5 24-25 0.0 0.0 0.0 9.756532242249492E-5 3.252177414083164E-5 26-27 0.0 0.0 0.0 1.463479836337424E-4 3.252177414083164E-5 28-29 0.0 0.0 0.0 3.7400040261956387E-4 3.252177414083164E-5 30-31 0.0 0.0 0.0 0.0016748713682528296 3.252177414083164E-5 32-33 0.0 0.0 0.0 0.005528701603941378 3.252177414083164E-5 34-35 0.0 0.0 0.0 0.011659056029488142 3.252177414083164E-5 36-37 0.0 0.0 0.0 0.0220985455286951 3.252177414083164E-5 38-39 0.0 0.0 0.0 0.04112378340108161 3.252177414083164E-5 40-41 0.0 0.0 0.0 0.06073441320800309 3.252177414083164E-5 42-43 0.0 0.0 0.0 0.08475174341100726 3.252177414083164E-5 44-45 0.0 0.0 0.0 0.11637916876296603 3.252177414083164E-5 46-47 6.504354828166328E-5 1.626088707041582E-5 0.0 0.15629964652083686 3.252177414083164E-5 48-49 6.504354828166328E-5 3.252177414083164E-5 0.0 0.1980576045176647 3.252177414083164E-5 50-51 6.504354828166328E-5 3.252177414083164E-5 0.0 0.2404172153360979 3.252177414083164E-5 52-53 6.504354828166328E-5 3.252177414083164E-5 0.0 0.28656561284193804 1.463479836337424E-4 54-55 6.504354828166328E-5 3.252177414083164E-5 0.0 0.343072195411633 2.6017419312665313E-4 56-57 6.504354828166328E-5 3.252177414083164E-5 0.0 0.4182950589993766 2.6017419312665313E-4 58-59 6.504354828166328E-5 3.252177414083164E-5 0.0 0.5022662798310038 2.6017419312665313E-4 60-61 6.504354828166328E-5 3.252177414083164E-5 0.0 0.5847902817133641 2.6017419312665313E-4 62-63 6.504354828166328E-5 3.252177414083164E-5 0.0 0.6703225477037513 2.6017419312665313E-4 64-65 6.504354828166328E-5 3.252177414083164E-5 0.0 0.7706196991540761 2.6017419312665313E-4 66-67 6.504354828166328E-5 3.252177414083164E-5 0.0 0.8967716610463621 2.7643508019706893E-4 68-69 6.504354828166328E-5 3.252177414083164E-5 0.0 1.0508273051514816 2.9269596726748474E-4 70-71 6.504354828166328E-5 3.252177414083164E-5 0.0 1.2046227750634744 2.9269596726748474E-4 72-73 6.504354828166328E-5 3.252177414083164E-5 0.0 1.3539302401440323 3.9026128968997967E-4 74-75 6.504354828166328E-5 3.252177414083164E-5 0.0 1.517335894314641 3.9026128968997967E-4 76-77 6.504354828166328E-5 3.252177414083164E-5 0.0 1.7132795835131516 4.3904395090122713E-4 78-79 6.504354828166328E-5 3.252177414083164E-5 0.0 1.9526886238508836 4.878266121124746E-4 80-81 6.504354828166328E-5 3.252177414083164E-5 0.0 2.201528978689457 4.878266121124746E-4 82-83 1.78869757774574E-4 3.252177414083164E-5 0.0 2.4478651569191863 4.878266121124746E-4 84-85 1.9513064484498983E-4 3.252177414083164E-5 0.0 2.7076653496432197 4.878266121124746E-4 86-87 1.9513064484498983E-4 3.252177414083164E-5 0.0 3.0025565366652107 4.878266121124746E-4 88-89 1.9513064484498983E-4 3.252177414083164E-5 0.0 3.3509623030359403 4.878266121124746E-4 90-91 2.9269596726748474E-4 3.252177414083164E-5 0.0 3.723482964932096 4.878266121124746E-4 92-93 2.9269596726748474E-4 3.252177414083164E-5 0.0 4.095044234491098 4.878266121124746E-4 94-95 2.9269596726748474E-4 3.252177414083164E-5 0.0 4.475581513712969 4.878266121124746E-4 96-97 2.9269596726748474E-4 3.252177414083164E-5 0.0 4.885827433612489 4.878266121124746E-4 98-99 3.57739515549148E-4 3.252177414083164E-5 0.0 5.353669415515423 4.878266121124746E-4 100-101 3.9026128968997967E-4 3.252177414083164E-5 0.0 5.8674158816181405 4.878266121124746E-4 102-103 3.9026128968997967E-4 3.252177414083164E-5 0.0 6.368413812257652 4.878266121124746E-4 104-105 3.9026128968997967E-4 3.252177414083164E-5 0.0 6.875802271515837 4.878266121124746E-4 106-107 4.2278306383081133E-4 3.252177414083164E-5 0.0 7.41912729119964 4.878266121124746E-4 108-109 4.5530483797164294E-4 3.252177414083164E-5 0.0 7.998112436228865 4.878266121124746E-4 110-111 4.5530483797164294E-4 3.252177414083164E-5 0.0 8.621408498524975 4.878266121124746E-4 112-113 4.5530483797164294E-4 3.252177414083164E-5 0.0 9.247111172107505 4.878266121124746E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6610 0.0 85.98381 1 GTATCAA 10485 0.0 54.108944 2 GTACATG 19300 0.0 45.12943 1 TACATGG 19430 0.0 44.27307 2 ACATGGG 19455 0.0 43.63479 3 TCAACGC 14840 0.0 39.834553 5 ATCAACG 14860 0.0 39.740875 4 CAACGCA 15120 0.0 39.19012 6 CATGGGA 12600 0.0 38.695274 4 AACGCAG 15540 0.0 38.169212 7 TATCAAC 16005 0.0 37.344154 3 CATGGGG 6870 0.0 36.30796 4 ATGGGAG 4755 0.0 32.92682 5 ACGCAGA 18230 0.0 32.406467 8 CGCAGAG 18505 0.0 31.796276 9 GAGTACA 12735 0.0 29.89315 1 ATGGGAT 5860 0.0 29.562418 5 AGTACAT 12455 0.0 29.299574 2 CAGAGTA 20635 0.0 27.187939 9 GCAGAGT 20850 0.0 26.67931 8 >>END_MODULE