FastQCFastQC Report
Thu 26 May 2016
SRR936183_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936183_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2582628
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145270.5624890615295738No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41890.16219912430284192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC2850.026.1515454
TCGCGCT3500.025.35393137
ATTCGCG3700.024.94520656
CGCGGAA3750.024.6145159
AATTCGC3700.023.0254555
TCGCGGA4100.022.51239858
CTCGCGC4000.022.18383436
CGATCGT4400.021.7792241
CAATTCG4000.021.30101854
CGCGCTT4350.020.3993238
CGCTCCG4650.019.84810425
TAAGGCG10550.019.51449617
CCTCGCG4750.018.68003735
ACGATCG2507.185008E-1018.46088259
ACGCTCC5250.018.2576624
GCGTAAG6050.018.18742838
GATCGTT5300.018.0808662
ATCGTTC5300.018.0805153
TTGCGTA6000.017.74706636
GATTCGT3400.017.74637833
Produced by FastQC (version 0.11.5)