##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936183_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2582628 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.61544906970729 40.0 36.0 41.0 30.0 41.0 2 36.471302100031444 39.0 36.0 41.0 30.0 41.0 3 36.376146312980424 39.0 36.0 41.0 30.0 41.0 4 36.254205406276085 39.0 36.0 41.0 29.0 41.0 5 36.17443123825809 39.0 35.0 41.0 29.0 41.0 6 36.052539119067866 39.0 35.0 40.0 29.0 41.0 7 35.85773560884494 39.0 35.0 40.0 28.0 41.0 8 35.65276958199168 38.0 35.0 40.0 27.0 41.0 9 35.530381069205475 38.0 35.0 40.0 27.0 41.0 10-11 35.72198028519787 39.0 35.0 40.0 28.0 41.0 12-13 35.86278047012578 39.0 35.0 41.0 28.5 41.0 14-15 35.77607189266128 39.0 35.0 41.0 27.5 41.0 16-17 35.573804860785216 39.0 35.0 41.0 27.0 41.0 18-19 35.37330656989702 39.0 35.0 41.0 26.0 41.0 20-21 35.099128871831326 39.0 35.0 41.0 25.0 41.0 22-23 34.74668806347643 39.0 34.0 41.0 23.5 41.0 24-25 34.436567519596316 39.0 34.0 40.5 22.5 41.0 26-27 33.47759259173215 37.5 33.0 39.5 20.0 40.5 28-29 33.499090074141535 38.0 33.0 39.5 20.0 40.5 30-31 33.8473502571799 38.0 33.0 40.0 19.5 41.0 32-33 33.678010538103045 38.0 33.0 40.0 18.5 41.0 34-35 33.3992322936172 38.0 33.0 40.0 16.0 41.0 36-37 33.10179282498292 37.0 33.0 40.0 13.5 41.0 38-39 32.812769396134485 37.0 32.0 40.0 11.0 41.0 40-41 32.43206629061561 36.0 32.0 39.5 8.0 41.0 42-43 32.072944690447095 36.0 31.5 39.0 7.0 41.0 44-45 31.697802780733426 35.0 31.0 39.0 2.0 40.5 46-47 31.310674824248785 35.0 31.0 38.5 2.0 40.0 48-49 30.85591691873549 35.0 31.0 37.5 2.0 40.0 50-51 30.427046403895567 35.0 30.0 37.0 2.0 39.0 52-53 29.990578588941187 35.0 30.0 36.0 2.0 39.0 54-55 29.53936048861857 34.0 29.0 36.0 2.0 38.5 56-57 29.093692161627615 34.0 29.0 35.5 2.0 37.0 58-59 28.671033149179827 34.0 29.0 35.0 2.0 37.0 60-61 28.33203852819686 34.0 28.5 35.0 2.0 36.0 62-63 27.993457633077625 34.0 27.5 35.0 2.0 36.0 64-65 27.618247769326437 34.0 27.0 35.0 2.0 36.0 66-67 27.23720586162622 34.0 26.0 35.0 2.0 35.0 68-69 26.875267556922637 33.0 25.5 35.0 2.0 35.0 70-71 26.45733744852143 33.0 25.0 35.0 2.0 35.0 72-73 25.976168848165514 33.0 24.0 35.0 2.0 35.0 74-75 25.399193573367903 33.0 20.0 35.0 2.0 35.0 76-77 23.728773559335686 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0003286415339758 1101 2 1.045692697547139 1101 3 1.0705982747228688 1101 4 0.9816924454040503 1101 5 1.0413611043557367 1101 6 0.919869371988888 1101 7 0.9446116933679676 1101 8 1.0049156016179452 1101 9 1.2149773847844614 1101 10-11 1.0268405196092232 1101 12-13 1.0966875367385143 1101 14-15 1.1425181405156764 1101 16-17 1.0744497802034303 1101 18-19 1.0543824658236574 1101 20-21 0.9612961653403218 1101 22-23 0.9588543937518281 1101 24-25 0.9493532941688798 1101 26-27 0.6744710414507296 1101 28-29 0.8282801928947663 1101 30-31 0.8534042900684469 1101 32-33 0.8120977409150996 1101 34-35 0.8180861461933588 1101 36-37 0.9591398047986743 1101 38-39 0.9302426660737737 1101 40-41 0.9020178189554642 1101 42-43 0.8430907793615461 1101 44-45 0.758318361427488 1101 46-47 0.679707601239997 1101 48-49 0.7043277249615159 1101 50-51 0.7542306272539179 1101 52-53 0.8202569837985969 1101 54-55 0.8907574498226118 1101 56-57 0.8273779066064719 1101 58-59 0.8222549932609873 1101 60-61 0.7931868980101626 1101 62-63 0.8581401336791288 1101 64-65 0.6806069957535996 1101 66-67 0.6917698799311864 1101 68-69 0.7007841777468222 1101 70-71 0.7444687541325763 1101 72-73 0.7153256230755893 1101 74-75 0.6946796587852511 1101 76-77 0.6617396995980407 1102 1 0.8269575613761049 1102 2 0.8482012266735452 1102 3 0.9746771821454914 1102 4 0.8307412381637889 1102 5 0.910888292472066 1102 6 1.082092613247987 1102 7 0.9509270682992366 1102 8 0.9895329214235673 1102 9 0.8473003855083974 1102 10-11 0.8656872670770213 1102 12-13 0.9471032719417778 1102 14-15 0.8231273016572516 1102 16-17 0.9373253642920218 1102 18-19 0.9437726465540877 1102 20-21 0.9862243550849854 1102 22-23 1.0051950959956173 1102 24-25 1.0063840648367446 1102 26-27 0.822736396532008 1102 28-29 0.8243080720454898 1102 30-31 0.9114312754388934 1102 32-33 0.8408563416925432 1102 34-35 0.9027222014166725 1102 36-37 0.8624340007467239 1102 38-39 0.8100175438424202 1102 40-41 0.9028601166288652 1102 42-43 0.9893536208185125 1102 44-45 0.9076802426544965 1102 46-47 0.8283160851466391 1102 48-49 0.7808035238970454 1102 50-51 0.90116831527153 1102 52-53 0.9757322901996339 1102 54-55 0.9207749857489738 1102 56-57 0.9380158555399838 1102 58-59 1.057919479613325 1102 60-61 1.0820957961128457 1102 62-63 1.0405012952113353 1102 64-65 0.9713705940577526 1102 66-67 0.9959648620237402 1102 68-69 1.1724735285990988 1102 70-71 1.0750167199863476 1102 72-73 1.04372220038546 1102 74-75 1.0841850119133731 1102 76-77 1.2612404246003273 1103 1 0.706935045761135 1103 2 0.5616683832783025 1103 3 0.7306722109736086 1103 4 0.7898858489063585 1103 5 0.9550633096763832 1103 6 0.8657298000489391 1103 7 0.6284471904424009 1103 8 0.7262686182630205 1103 9 0.7425311608841696 1103 10-11 0.8922110345468184 1103 12-13 0.8776291565409764 1103 14-15 1.0330295445440072 1103 16-17 1.1075898488654943 1103 18-19 1.1033218908398865 1103 20-21 1.1667350632869429 1103 22-23 1.0743429848399941 1103 24-25 1.0742735884069745 1103 26-27 0.9150201531064681 1103 28-29 0.9229476158069119 1103 30-31 1.1980513941156872 1103 32-33 1.2384393266725695 1103 34-35 1.2277897209880635 1103 36-37 1.178299168882731 1103 38-39 1.0736540866362532 1103 40-41 1.2960417704497047 1103 42-43 1.3422547409158057 1103 44-45 1.263722569054547 1103 46-47 1.0295750838704194 1103 48-49 1.083232609651521 1103 50-51 1.009135932622911 1103 52-53 1.041977484120558 1103 54-55 1.1059917847626188 1103 56-57 1.1230396283765032 1103 58-59 1.2864339681018713 1103 60-61 1.2622074310516034 1103 62-63 1.1147606826268373 1103 64-65 1.1010650056419955 1103 66-67 1.0938070510707973 1103 68-69 1.0145948317387017 1103 70-71 0.8804380634027922 1103 72-73 0.9804316090655156 1103 74-75 0.996498955881588 1103 76-77 1.2556244537868686 1104 1 0.8359353450293767 1104 2 0.7313782999520981 1104 3 0.8763627483049063 1104 4 0.7566932773784885 1104 5 0.8377623989079694 1104 6 0.7145810651123412 1104 7 0.9049050198236515 1104 8 0.8449601954480244 1104 9 0.7430079903509252 1104 10-11 0.7151406016973709 1104 12-13 0.851069962110401 1104 14-15 0.8373984471259917 1104 16-17 0.9495593235049284 1104 18-19 0.8344257562270982 1104 20-21 0.854229374432137 1104 22-23 0.9764794256441789 1104 24-25 0.8796144232136598 1104 26-27 0.6551172359996258 1104 28-29 0.7309579026355166 1104 30-31 1.0022178251833154 1104 32-33 0.893528972680997 1104 34-35 0.9295880541880805 1104 36-37 0.8344309958532818 1104 38-39 0.8475014212181549 1104 40-41 0.9336162082529356 1104 42-43 0.846013436244192 1104 44-45 0.8083793035553448 1104 46-47 0.7012273134877702 1104 48-49 0.7638513363961721 1104 50-51 0.7130874111735146 1104 52-53 0.7803093843221838 1104 54-55 0.9143907238084381 1104 56-57 0.9133988640426587 1104 58-59 0.8963069583846206 1104 60-61 0.7879593252678632 1104 62-63 0.780933252141196 1104 64-65 0.7908512357172164 1104 66-67 0.7717066776060975 1104 68-69 0.6373246338906782 1104 70-71 0.6798815382518484 1104 72-73 0.5001271139737895 1104 74-75 0.514031661625058 1104 76-77 0.49389024260002756 1105 1 0.2772212847089506 1105 2 0.3977104550462869 1105 3 0.31557982870957346 1105 4 0.41667512978710874 1105 5 0.3382792328304234 1105 6 0.08185208720093584 1105 7 0.13654734298620497 1105 8 0.37072368086900553 1105 9 0.20583556114684143 1105 10-11 0.18454108789030244 1105 12-13 0.17038038749536355 1105 14-15 0.03974070528224871 1105 16-17 0.15393539847885762 1105 18-19 0.27225891350170883 1105 20-21 0.2674547702256902 1105 22-23 0.09787978656455465 1105 24-25 0.04202961030991048 1105 26-27 0.02203529420735606 1105 28-29 0.16933413335370773 1105 30-31 0.2866705703450876 1105 32-33 0.24827299345592735 1105 34-35 0.3589954473001242 1105 36-37 0.41488915482076294 1105 38-39 0.4193682531091554 1105 40-41 0.462673346079832 1105 42-43 0.34192929710362563 1105 44-45 0.20101471641305224 1105 46-47 0.06680258536306383 1105 48-49 0.09636125662802897 1105 50-51 0.3519677792716749 1105 52-53 0.20844331422372875 1105 54-55 0.25628601288331865 1105 56-57 0.22908865873110074 1105 58-59 0.19660270318073003 1105 60-61 -0.06997990738696558 1105 62-63 -0.0465533233812252 1105 64-65 0.08647837720070939 1105 66-67 0.04914289017668949 1105 68-69 -0.32488249919617473 1105 70-71 -0.4842916121158467 1105 72-73 -0.7104692510907391 1105 74-75 -0.8368262860719611 1105 76-77 -0.5578832104532374 1106 1 -0.12855424700090623 1106 2 -0.14753988724545053 1106 3 -0.3834467361331235 1106 4 -0.35820779016793836 1106 5 -0.3227811081347056 1106 6 -0.11519681984339059 1106 7 -0.10877283432967744 1106 8 -0.07985289611346502 1106 9 -0.10820728601957796 1106 10-11 -0.29064244806472317 1106 12-13 -0.1594536854884936 1106 14-15 -0.10044622230208233 1106 16-17 -0.030927422005085248 1106 18-19 0.011314463634001015 1106 20-21 -0.07808167039076608 1106 22-23 -0.08747988884024238 1106 24-25 -0.08572443023445686 1106 26-27 -0.038638826534651116 1106 28-29 -0.09921632683156645 1106 30-31 -0.14090400169637718 1106 32-33 -0.14453825423814237 1106 34-35 -0.1345864887069652 1106 36-37 -0.07790306971260463 1106 38-39 -0.10189989085611728 1106 40-41 -0.16733393778827832 1106 42-43 -0.0751517490700877 1106 44-45 -0.21541428589726053 1106 46-47 -0.037252351522592875 1106 48-49 0.015606938245397828 1106 50-51 -0.09375163411412402 1106 52-53 -8.487488630812834E-4 1106 54-55 0.14569236511716 1106 56-57 0.010511290785629512 1106 58-59 -0.1665061318258907 1106 60-61 -0.1609306399038033 1106 62-63 -0.12776219518264398 1106 64-65 -0.21625645245439173 1106 66-67 -0.3011263125776651 1106 68-69 -0.41956102029561393 1106 70-71 -0.5014394896567502 1106 72-73 -0.5186376961726289 1106 74-75 -0.5457844426275678 1106 76-77 -0.5777766807533276 1107 1 0.4580807326754126 1107 2 0.3993766587883414 1107 3 0.3443771505688744 1107 4 0.40533169007856884 1107 5 0.3543038961542848 1107 6 0.01880061810562239 1107 7 0.15068408604355454 1107 8 0.20653829012002944 1107 9 0.3140902379036419 1107 10-11 0.4104966211388472 1107 12-13 0.4514962307247288 1107 14-15 0.3387766038676503 1107 16-17 0.39124606637349046 1107 18-19 0.4016960459030301 1107 20-21 0.330274695800405 1107 22-23 0.3394664160023453 1107 24-25 0.21775530554958067 1107 26-27 -0.00917305272946578 1107 28-29 0.25300808174329603 1107 30-31 0.5317112671034749 1107 32-33 0.5370625836620704 1107 34-35 0.5768164205463293 1107 36-37 0.5242773471570032 1107 38-39 0.3930338526127741 1107 40-41 0.500117531178212 1107 42-43 0.5730060759929643 1107 44-45 0.5711678551231394 1107 46-47 0.5364662422076094 1107 48-49 0.6721618319539822 1107 50-51 0.5772156875833652 1107 52-53 0.6000461866303333 1107 54-55 0.5187464815579368 1107 56-57 0.6144461908393914 1107 58-59 0.595462771628597 1107 60-61 0.5962778425395356 1107 62-63 0.6748436206666213 1107 64-65 0.6582467167307371 1107 66-67 0.584308592823156 1107 68-69 0.4943083248258766 1107 70-71 0.4444779342667644 1107 72-73 0.4113918357912745 1107 74-75 0.4552758951963263 1107 76-77 0.45231672425002856 1108 1 -0.17158290277166088 1108 2 -0.1116931740191589 1108 3 -0.11086891675567756 1108 4 -0.19988105678180546 1108 5 -0.24008676968941245 1108 6 -0.34786895846627885 1108 7 -0.19219916691901773 1108 8 0.08882975442532626 1108 9 -0.033840880950819496 1108 10-11 -0.24849578523802052 1108 12-13 -0.22176618698848216 1108 14-15 -0.16134573510598216 1108 16-17 -0.19495710976758573 1108 18-19 -0.25962986881133787 1108 20-21 -0.38185924017020767 1108 22-23 -0.21449137274837682 1108 24-25 -0.07422402223771485 1108 26-27 -0.27146996388555067 1108 28-29 -0.1854251371610829 1108 30-31 -0.039887309829907736 1108 32-33 -0.10753655025177267 1108 34-35 -0.2065964126275759 1108 36-37 -0.13725159492440042 1108 38-39 -0.1174817662011236 1108 40-41 -0.10537693344431176 1108 42-43 -0.05863988116503549 1108 44-45 -0.09379825882547621 1108 46-47 -0.024455953893770754 1108 48-49 -0.03477315871382913 1108 50-51 0.05712765885153104 1108 52-53 0.018363097442151144 1108 54-55 0.08103838234902483 1108 56-57 -0.016551492712103766 1108 58-59 0.012605935277569813 1108 60-61 -3.1492482952444334E-4 1108 62-63 0.06258178206553922 1108 64-65 -0.05528674021209312 1108 66-67 -0.21094253404789143 1108 68-69 -0.1781739513921643 1108 70-71 -0.12716398386079675 1108 72-73 -0.03252158510151659 1108 74-75 0.04796180740243372 1108 76-77 0.07750207701661083 1109 1 0.18891484859219076 1109 2 0.15761442801742476 1109 3 0.09396246338576475 1109 4 0.31729287859760547 1109 5 0.2413514599722859 1109 6 0.2977560732547744 1109 7 0.07472765368400758 1109 8 0.2118313277897883 1109 9 0.3197723177325571 1109 10-11 0.16076450445414991 1109 12-13 0.2327750615275903 1109 14-15 0.33039131620351725 1109 16-17 0.36628170739905386 1109 18-19 0.11370957250191793 1109 20-21 0.2854509255467903 1109 22-23 0.22378091895414087 1109 24-25 0.2262886674232547 1109 26-27 0.22076332855598935 1109 28-29 0.3597138505874682 1109 30-31 0.3977477754548673 1109 32-33 0.4146264111890403 1109 34-35 0.561309041289924 1109 36-37 0.5463905828793969 1109 38-39 0.5642316165572367 1109 40-41 0.6641074554626627 1109 42-43 0.5827079140821922 1109 44-45 0.4189689253225346 1109 46-47 0.1588881233360162 1109 48-49 0.5014649002421585 1109 50-51 0.4618129209695958 1109 52-53 0.43804537932815535 1109 54-55 0.5510586559733177 1109 56-57 0.5990036784083017 1109 58-59 0.5526025527296632 1109 60-61 0.5370083811422042 1109 62-63 0.51126322280194 1109 64-65 0.4993017958904957 1109 66-67 0.5446413778981665 1109 68-69 0.6038663284711241 1109 70-71 0.6513441949110366 1109 72-73 0.5266380327850761 1109 74-75 0.3620434502870751 1109 76-77 0.34625525685922653 1110 1 -0.2836826191673367 1110 2 -0.43550514574227606 1110 3 -0.43829614741279954 1110 4 -0.3335174119788178 1110 5 -0.26558969160306845 1110 6 -0.5017069001765364 1110 7 -0.46667512553413815 1110 8 -0.3618469969822513 1110 9 -0.5690483159330171 1110 10-11 -0.45092185548463704 1110 12-13 -0.41403982571091547 1110 14-15 -0.3644877786353078 1110 16-17 -0.5634263194351945 1110 18-19 -0.5479991323219977 1110 20-21 -0.6145035604249358 1110 22-23 -0.6785815993069235 1110 24-25 -0.6503060470148014 1110 26-27 -0.474549925452763 1110 28-29 -0.34818402474515864 1110 30-31 -0.3503681955480431 1110 32-33 -0.17407971056015725 1110 34-35 -0.13173165847214108 1110 36-37 -0.21476005431699718 1110 38-39 -0.14510533831599304 1110 40-41 -0.1311841730133736 1110 42-43 -0.11076552113142668 1110 44-45 -0.1586710082092999 1110 46-47 -0.2852788125882668 1110 48-49 -0.21299219548027182 1110 50-51 -0.2392176862777582 1110 52-53 -0.20415606093531835 1110 54-55 -0.2588003089864266 1110 56-57 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-0.7118408684787099 2314 20-21 -0.7455861802720349 2314 22-23 -0.6951564317254224 2314 24-25 -0.6241185415939583 2314 26-27 -0.6662355908845043 2314 28-29 -0.7323718275379747 2314 30-31 -0.858864422641112 2314 32-33 -1.038592763950824 2314 34-35 -0.992586293138281 2314 36-37 -0.995445310862273 2314 38-39 -0.8040680057426073 2314 40-41 -0.671362720103474 2314 42-43 -0.9000031337300882 2314 44-45 -0.8057148210136411 2314 46-47 -0.7402727412437713 2314 48-49 -0.7111166456518809 2314 50-51 -0.5398671342810317 2314 52-53 -0.5643066054977481 2314 54-55 -0.5611089067536561 2314 56-57 -0.47681848859266296 2314 58-59 -0.5872682427537974 2314 60-61 -0.6381199807361853 2314 62-63 -0.7123884735443156 2314 64-65 -0.61097501900338 2314 66-67 -0.6124380052246181 2314 68-69 -0.6073118118429974 2314 70-71 -0.47961281071646056 2314 72-73 -0.33659036180750235 2314 74-75 -0.332514230772734 2314 76-77 -0.2424099051751334 2315 1 -1.491261838288466 2315 2 -1.4798795334309034 2315 3 -1.4433982806980694 2315 4 -1.3413768972798223 2315 5 -1.2901621938005903 2315 6 -1.3522951218481083 2315 7 -1.4235102573302285 2315 8 -1.4396401185544363 2315 9 -1.5131725432753598 2315 10-11 -1.4805413640647984 2315 12-13 -1.5909037946666658 2315 14-15 -1.8576355549821812 2315 16-17 -1.707389092917495 2315 18-19 -1.5710863546611264 2315 20-21 -1.5379757145426467 2315 22-23 -1.4715588094929686 2315 24-25 -1.7560287481222758 2315 26-27 -1.6001462525410588 2315 28-29 -1.4740929893360644 2315 30-31 -1.7321425115465203 2315 32-33 -1.6516562765417788 2315 34-35 -1.5492231729566726 2315 36-37 -1.7193107927154259 2315 38-39 -1.6972000095834474 2315 40-41 -1.6559356488536991 2315 42-43 -1.8115903102143847 2315 44-45 -1.818784327976548 2315 46-47 -1.8227262154818504 2315 48-49 -1.7238798846108558 2315 50-51 -1.6602755841253405 2315 52-53 -1.5922670759449424 2315 54-55 -1.428718539931392 2315 56-57 -1.6768469618594324 2315 58-59 -1.566924787442396 2315 60-61 -1.589819329680786 2315 62-63 -1.731178886989472 2315 64-65 -1.7820651066433193 2315 66-67 -1.7193388594226207 2315 68-69 -1.6254147596309103 2315 70-71 -1.6821142954335926 2315 72-73 -2.999170716918538 2315 74-75 -3.46403041835217 2315 76-77 -3.1348919081834232 2316 1 -3.4446137392067016 2316 2 -3.420228123204133 2316 3 -2.994782888722341 2316 4 -2.7243636874240167 2316 5 -3.404027462376817 2316 6 -3.0310357344189356 2316 7 -3.386907225110548 2316 8 -3.3397505672193475 2316 9 -2.9678754726342547 2316 10-11 -3.0192138297579376 2316 12-13 -3.2490802239338663 2316 14-15 -3.1893091146161083 2316 16-17 -4.413752353277715 2316 18-19 -4.247364764519304 2316 20-21 -3.581710903708487 2316 22-23 -3.7376589401845486 2316 24-25 -4.00372396176482 2316 26-27 -3.7676517228880115 2316 28-29 -3.930503191664954 2316 30-31 -3.9742483514103633 2316 32-33 -4.062498173676367 2316 34-35 -4.199392652754781 2316 36-37 -4.123499791112714 2316 38-39 -3.983943674796926 2316 40-41 -4.09774689892733 2316 42-43 -4.333488609989143 2316 44-45 -4.051111062598036 2316 46-47 -3.7098102035377565 2316 48-49 -3.7594530228988106 2316 50-51 -4.084906652403657 2316 52-53 -5.118367903692608 2316 54-55 -4.142845551780578 2316 56-57 -4.037775849535404 2316 58-59 -4.096237796232245 2316 60-61 -3.919511423804451 2316 62-63 -3.8528298990454566 2316 64-65 -3.9491726634257382 2316 66-67 -4.073072700775228 2316 68-69 -4.066135605646075 2316 70-71 -3.857449300329705 2316 72-73 -3.891961819499212 2316 74-75 -3.9751493631275814 2316 76-77 -3.989261781059426 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64622.0 3 6211.0 4 8557.0 5 10330.0 6 11702.0 7 12393.0 8 15010.0 9 16332.0 10 20926.0 11 23904.0 12 17933.0 13 13886.0 14 13253.0 15 13426.0 16 13618.0 17 14260.0 18 15039.0 19 15390.0 20 16167.0 21 17685.0 22 19290.0 23 21970.0 24 25533.0 25 30050.0 26 35528.0 27 41311.0 28 48726.0 29 57672.0 30 68332.0 31 82421.0 32 99712.0 33 126748.0 34 169799.0 35 226349.0 36 312483.0 37 412366.0 38 362522.0 39 100240.0 40 932.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.117418360511 26.302688594673928 29.910819047471527 21.669073997343546 2 21.719986725007445 26.55920170359823 29.960937908429656 21.75987366296467 3 21.961007553358545 26.67352103458358 29.369068987083196 21.996402424974683 4 21.705043301267068 26.75953632810367 29.723029117551043 21.812391253078218 5 21.971797497138237 26.668806857698723 29.638844699580684 21.72055094558235 6 21.987797378868308 26.605369587003697 29.575169726099325 21.831663308028666 7 21.858186751203515 26.38416374702921 29.692625783867953 22.06502371789932 8 21.878325647392693 26.748157775295834 29.334514236675336 22.039002340636138 9 21.601201146774514 26.60217125016314 29.787363406151112 22.00926419691124 10-11 22.297321371171684 26.620360150603645 29.355957185933228 21.726361292291436 12-13 22.353349396993135 26.350888729336624 29.28022179313507 22.015540080535168 14-15 21.988173883411683 26.635016767218346 29.429430534150136 21.94737881521983 16-17 22.11795109888684 26.520817029541792 29.545413182769874 21.81581868880149 18-19 22.07879946750575 26.600334019121384 29.08315139779741 22.237715115575458 20-21 21.985830160607907 26.867234681502 29.074409762692856 22.072525395197236 22-23 22.268194752521932 27.046635535644953 28.530700331803892 22.15446938002922 24-25 22.29164113077109 27.228012940381646 28.27442795237069 22.205917976476574 26-27 22.341159324982563 27.335510684006465 28.086681174591998 22.236648816418974 28-29 22.6186096761952 27.291358493196306 28.012755477420402 22.077276353188093 30-31 22.146173800610978 27.283759916574347 28.209713450838148 22.36035283197653 32-33 22.191019122203702 27.67171326898275 27.929790701506313 22.207476907307235 34-35 22.2567044652547 27.265577274764723 28.12383402182721 22.35388423815337 36-37 22.19488275687141 27.40421263985579 28.428958534821515 21.971946068451285 38-39 22.16832906092877 27.30092656866373 28.498516518124852 22.032227852282656 40-41 21.94088292816951 27.26546996621876 28.40524553115466 22.38840157445707 42-43 21.94400044147503 27.604988280580617 28.141961330272697 22.309049947671657 44-45 22.069803587012064 27.260703054674178 28.364859309871843 22.304634048441915 46-47 21.997772674539185 27.457431108699566 28.194140983776077 22.350655232985176 48-49 22.09880538048373 27.55899366991662 28.014080364115827 22.32812058548382 50-51 21.9487816314029 27.75200782581935 27.880546637587756 22.41866390518999 52-53 21.969629769364584 27.596841812096105 28.046096869120667 22.387431549418636 54-55 22.02054864156457 27.573155274800076 28.016816926147087 22.389479157488267 56-57 22.028548551223476 27.90934097055005 27.806904671308253 22.255205806918227 58-59 22.12533575604361 27.866299387506622 27.723167399366126 22.28519745708364 60-61 22.01717506107394 27.669457917459944 27.683496791003968 22.62987023046215 62-63 22.056899279178197 27.638151057123334 27.75151351664331 22.553436147055162 64-65 21.99543139186853 27.65638458726057 27.634889355716258 22.713294665154642 66-67 22.002751060405394 27.659931287395956 27.834956515777932 22.50236113642072 68-69 22.171638436265688 27.402983236074952 27.888548844471998 22.53682948318736 70-71 21.92605302725359 27.25463844156267 28.00643963798247 22.812868893201266 72-73 22.088065869776514 26.999853509039813 27.950591466940324 22.961489154243356 74-75 21.910916839672712 27.151207606091106 27.774378156787076 23.16349739744911 76-77 21.813399291379902 27.020171876417027 28.15577007595459 23.010658756248485 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14629.0 1 8850.0 2 2939.5 3 2948.0 4 3088.0 5 3161.0 6 3339.5 7 3649.5 8 3910.5 9 3967.0 10 4064.0 11 4188.0 12 4483.5 13 4752.0 14 4926.5 15 5380.5 16 6034.0 17 6408.0 18 7012.0 19 8335.0 20 10030.0 21 12768.5 22 14531.0 23 16326.5 24 20246.5 25 25601.5 26 28832.0 27 30433.0 28 36437.0 29 42188.0 30 43536.0 31 48655.5 32 58358.5 33 67018.0 34 74184.0 35 77274.0 36 82122.0 37 89453.5 38 92241.5 39 92546.0 40 95089.5 41 99976.5 42 106075.0 43 109830.0 44 108724.0 45 106964.5 46 108278.5 47 111646.0 48 113046.0 49 114031.5 50 114362.0 51 110355.0 52 107003.0 53 106296.5 54 100717.5 55 90251.0 56 79856.0 57 75055.0 58 71558.0 59 61761.0 60 51054.0 61 46647.0 62 41438.0 63 30906.0 64 22378.0 65 19173.0 66 16716.5 67 12884.0 68 9924.0 69 7118.0 70 5896.0 71 5009.5 72 3546.5 73 2708.0 74 2446.0 75 2087.0 76 1379.5 77 809.0 78 587.0 79 488.0 80 346.5 81 267.0 82 190.0 83 150.0 84 125.0 85 91.5 86 73.0 87 63.0 88 55.0 89 49.0 90 45.5 91 40.0 92 42.5 93 44.0 94 40.0 95 33.5 96 30.0 97 39.5 98 45.5 99 86.5 100 131.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00921541933255583 2 0.01273896201853306 3 0.012893843015718873 4 0.01436521248898409 5 0.011770955786121733 6 0.008983097836777114 7 0.015526819967877682 8 0.016417385701696104 9 0.016610986948178367 10-11 0.018818041158076195 12-13 0.015565540217174134 14-15 0.008053811853662239 16-17 0.0058467576437644135 18-19 0.01705626981508758 20-21 0.02301918820674135 22-23 0.020541092251768353 24-25 0.018527639288352795 26-27 0.022883667334203765 28-29 0.008053811853662239 30-31 0.008092532102958691 32-33 0.010202785689615385 34-35 0.011635434913584148 36-37 0.010861029927655086 38-39 0.013881209372778425 40-41 0.010744869179765727 42-43 0.014539453610818128 44-45 0.013145524636145817 46-47 0.02352255144759524 48-49 0.022941747708148445 50-51 0.035525828729495695 52-53 0.039533374531678586 54-55 0.02731713587864764 56-57 0.02073469349825062 58-59 0.015333218721395417 60-61 0.019824767639783972 62-63 0.024955200671564003 64-65 0.025555364535659028 66-67 0.02766561812231572 68-69 0.04268907484933951 70-71 0.05525379574603854 72-73 0.08766264440716975 74-75 0.08808856714943072 76-77 0.09300603881008027 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2582628.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.90542666014998 #Duplication Level Percentage of deduplicated Percentage of total 1 73.99217123344626 37.66603046149458 2 13.385568706144973 13.627961721501231 3 5.0776373490819156 7.7543788704158345 4 2.44492316842564 4.978394281599717 5 1.3881857282777263 3.533309339075435 6 0.8415255232521521 2.570292948393406 7 0.5805270447537599 2.0686383830662294 8 0.3976532189220866 1.6194165417608677 9 0.293848512799687 1.3462635525766748 >10 1.4234446473585218 13.181457640500192 >50 0.1101329819177932 3.880257340364943 >100 0.06055215730288588 5.38482812148577 >500 0.002527620688997016 0.8508960274183984 >1k 0.00122551306132452 0.9714555525376968 >5k 0.0 0.0 >10k+ 7.65945663327825E-5 0.5664192178091559 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14527 0.5624890615295738 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4189 0.16219912430284192 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.323214957787184E-4 0.0 3 0.0 0.0 0.0 3.0976199437162457E-4 0.0 4 0.0 0.0 0.0 0.0010454467310042328 0.0 5 0.0 0.0 0.0 0.00290401869723398 0.0 6 0.0 0.0 0.0 0.0050336324085388994 0.0 7 0.0 0.0 0.0 0.009563901576223909 0.0 8 0.0 0.0 0.0 0.013823128998833745 0.0 9 0.0 0.0 0.0 0.01788875517496132 0.0 10 0.0 0.0 0.0 0.022341583844053423 0.0 11 0.0 0.0 0.0 0.02938866921600788 0.0 12 0.0 0.0 0.0 0.037558641817559475 0.0 13 0.0 0.0 0.0 0.05025888358679609 0.0 14 0.0 0.0 0.0 0.06547594156030215 0.0 15 0.0 0.0 0.0 0.08286133349440956 0.0 16 0.0 0.0 0.0 0.10078880891866734 0.0 17 0.0 0.0 0.0 0.11813548060347832 0.0 18 0.0 0.0 0.0 0.13385590181783827 0.0 19 0.0 0.0 0.0 0.15201569873787477 0.0 20 0.0 0.0 0.0 0.17122094238891547 0.0 21 0.0 0.0 0.0 0.1937174072301547 0.0 22 0.0 0.0 0.0 0.21962125400948182 0.0 23 0.0 0.0 0.0 0.2457961425338841 0.0 24 0.0 0.0 0.0 0.27123534632165375 0.0 25 0.0 0.0 0.0 0.298688003072839 0.0 26 0.0 0.0 0.0 0.33009012525226245 0.0 27 0.0 0.0 0.0 0.35897543122741643 0.0 28 0.0 0.0 0.0 0.3872799334631236 0.0 29 0.0 0.0 0.0 0.4179850911552109 0.0 30 0.0 0.0 0.0 0.44845792735151946 0.0 31 0.0 0.0 0.0 0.48404183645495985 0.0 32 0.0 0.0 0.0 0.5250465804599036 0.0 33 0.0 0.0 0.0 0.5666708484535907 0.0 34 0.0 0.0 0.0 0.611392736390994 0.0 35 0.0 0.0 0.0 0.660877214991861 0.0 36 0.0 0.0 0.0 0.7139239565280017 0.0 37 0.0 0.0 0.0 0.7676289423021821 0.0 38 0.0 0.0 0.0 0.8198238383538009 0.0 39 0.0 0.0 0.0 0.8732577823829061 0.0 40 3.872024929645307E-5 0.0 0.0 0.9328869662994438 0.0 41 3.872024929645307E-5 0.0 0.0 0.9941811209357291 0.0 42 3.872024929645307E-5 0.0 0.0 1.057256407039651 0.0 43 3.872024929645307E-5 0.0 0.0 1.1276111000113063 0.0 44 3.872024929645307E-5 0.0 0.0 1.2065229680774776 0.0 45 3.872024929645307E-5 0.0 0.0 1.2872934081098788 0.0 46 3.872024929645307E-5 0.0 0.0 1.3753819752593095 0.0 47 3.872024929645307E-5 0.0 0.0 1.4693173000525046 0.0 48 3.872024929645307E-5 0.0 0.0 1.5610842908850984 0.0 49 3.872024929645307E-5 0.0 0.0 1.6547872941825148 0.0 50 3.872024929645307E-5 0.0 0.0 1.7515104769250547 0.0 51 3.872024929645307E-5 0.0 0.0 1.8505181543760851 0.0 52 3.872024929645307E-5 0.0 0.0 1.9609870256188657 0.0 53 3.872024929645307E-5 0.0 0.0 2.0808649174406844 0.0 54 3.872024929645307E-5 0.0 0.0 2.210306710838727 0.0 55 3.872024929645307E-5 0.0 0.0 2.346176065619981 0.0 56 3.872024929645307E-5 0.0 0.0 2.4957523886521793 0.0 57 3.872024929645307E-5 0.0 0.0 2.6417664487491037 0.0 58 3.872024929645307E-5 0.0 0.0 2.798970660892703 0.0 59 3.872024929645307E-5 0.0 0.0 2.958420647495497 0.0 60 3.872024929645307E-5 0.0 0.0 3.1182965568405514 0.0 61 3.872024929645307E-5 0.0 0.0 3.2853744325547467 0.0 62 3.872024929645307E-5 0.0 0.0 3.4652687107860674 0.0 63 3.872024929645307E-5 0.0 0.0 3.646556917992061 0.0 64 3.872024929645307E-5 0.0 0.0 3.8357440560545304 0.0 65 7.744049859290614E-5 0.0 0.0 4.049983195411805 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 285 0.0 26.151545 4 TCGCGCT 350 0.0 25.353931 37 ATTCGCG 370 0.0 24.945206 56 CGCGGAA 375 0.0 24.61451 59 AATTCGC 370 0.0 23.02545 55 TCGCGGA 410 0.0 22.512398 58 CTCGCGC 400 0.0 22.183834 36 CGATCGT 440 0.0 21.779224 1 CAATTCG 400 0.0 21.301018 54 CGCGCTT 435 0.0 20.39932 38 CGCTCCG 465 0.0 19.848104 25 TAAGGCG 1055 0.0 19.514496 17 CCTCGCG 475 0.0 18.680037 35 ACGATCG 250 7.185008E-10 18.460882 59 ACGCTCC 525 0.0 18.25766 24 GCGTAAG 605 0.0 18.187428 38 GATCGTT 530 0.0 18.080866 2 ATCGTTC 530 0.0 18.080515 3 TTGCGTA 600 0.0 17.747066 36 GATTCGT 340 0.0 17.746378 33 >>END_MODULE