FastQCFastQC Report
Thu 26 May 2016
SRR936183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936183_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2582628
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145690.5641153120000247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107380.4157780369453131No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71330.27619153823159975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13450.046.470238100-101
CGGTATC11300.041.352245100-101
GGTATCA103700.041.1406941
CGTACAT18200.041.046886100-101
TCGGTAT3350.038.203358100-101
CGAGTAC12700.037.73115100-101
TCGAGTA4650.036.483776100-101
AGTATCA36750.035.229767100-101
CCGTACA8200.034.481617100-101
AGGTATC24850.034.374264100-101
TACGCAG11500.033.386414100-101
CCGGTAT4950.033.070087100-101
TCGTATC6050.032.960766100-101
GCGTATC3550.032.69747100-101
CCTGTCG6650.032.2203296-97
GGGTATC16250.032.05255100-101
CCGTATC5300.032.00935100-101
GTATCAA204750.031.3275221
GCGTACA6700.030.207306100-101
GCGCGTA802.596371E-729.76250398-99
Produced by FastQC (version 0.11.5)