FastQCFastQC Report
Thu 26 May 2016
SRR936182_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936182_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2590657
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146140.5641040091374504No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41630.16069282811271426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC3000.030.7613284
TCGCGCT3650.026.25723837
ATTCGCG3300.025.81669456
CGCGGAA3200.025.51858959
CAATTCG3650.024.31460654
AATTCGC3650.024.31413855
CGATCGT3950.023.361231
CTCGCGC4200.022.81702836
CGCTCCG4050.021.91062525
ATACGTA2452.5465852E-1120.280631
CGCGCTT4600.020.06328638
TCGCGGA3900.020.02680658
AACTCGA4500.019.71918313
ACTCGAG4750.019.42858314
CCTCGCG5000.019.16482435
ACCGTTA2901.4551915E-1118.35959211
ATCGTTC5100.018.09423
TAACCGT3350.018.0124979
AGCTACG3400.017.75206244
TTGCGTA5000.017.74657836
Produced by FastQC (version 0.11.5)