FastQCFastQC Report
Thu 26 May 2016
SRR936182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936182_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2590657
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145510.5616721935786945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107000.4130226425188668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70880.273598550483526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13000.048.54177100-101
GGTATCA101650.041.2750971
CGTACAT18550.039.634754100-101
CGGTATC11050.039.329075100-101
TACGCAG12900.037.380787100-101
AGGTATC24000.036.091496100-101
AGTATCA40100.034.219975100-101
TCGTATC5550.033.78864100-101
CGAGTAC13350.032.776245100-101
CCGTATC5900.032.28874100-101
TCGAGTA5200.032.055885100-101
CCGTACA9200.032.031002100-101
GTATCAA202450.031.1009921
GGGTATC14700.030.576145100-101
GCGTACA5600.030.29772100-101
TCGGTAT3700.029.766182100-101
CCTGTCG8200.029.39721996-97
CCCGTAC4300.029.07141798-99
AAACGCA11450.028.8563100-101
GACGCAG10000.028.575535100-101
Produced by FastQC (version 0.11.5)