FastQCFastQC Report
Thu 26 May 2016
SRR936181_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936181_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738294
Sequences flagged as poor quality0
Sequence length77
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369905.010199188940991No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA237983.2233771370212954No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG13900.18827188084963442No Hit
GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC9490.12853957908367128No Hit
AGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG7460.10104375763584697No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGA200.006570698753.25238460
CGGTACA200.006577747453.2379539
CGCAATT200.00658127453.230742
TAACGGG305.4871937E-447.32262811
GTTAGCG400.002258032335.49437336
CGGTTAC903.938112E-935.484764
TCGCAAT450.00402571431.5441441
ATCACCG500.006727144628.3993516
CGCGCTT500.00673605528.39165538
GGCGGTT1455.0567905E-1026.9212952
GCGGTTA1259.620999E-825.5490253
GCCTAGT1201.9161143E-623.66612417
TTAGTCG750.001687476523.66612415
ATGGCGG750.001690807423.6581086
GGGCGGT1802.401066E-1023.6581081
GGCGACC750.001690807423.6581086
GTGGGTA2153.6379788E-1223.107921
CGTGTGC800.00245578322.1869955
CGAACTC800.002459652522.18097935
TAGTCGG850.00349095820.88187416
Produced by FastQC (version 0.11.5)