##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936181_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 738294 Sequences flagged as poor quality 0 Sequence length 77 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.84903304103785 40.0 36.0 41.0 31.0 41.0 2 36.65961798416349 39.0 36.0 41.0 30.0 41.0 3 36.526260541193615 39.0 35.0 41.0 30.0 41.0 4 36.38089297759429 39.0 35.0 41.0 30.0 41.0 5 36.21055297754011 39.0 35.0 41.0 30.0 41.0 6 35.94948083013 39.0 35.0 40.0 30.0 41.0 7 35.578615023283405 38.0 35.0 40.0 29.0 41.0 8 35.19012751017887 38.0 35.0 40.0 29.0 41.0 9 34.92911902304502 38.0 35.0 40.0 27.0 41.0 10-11 34.90176135794141 38.0 35.0 40.0 27.5 41.0 12-13 34.71195959875064 38.0 35.0 40.0 25.5 41.0 14-15 34.40428745188231 38.0 34.0 40.0 24.0 41.0 16-17 33.9646292669316 38.0 33.5 40.0 21.5 41.0 18-19 33.462347655541016 37.0 33.0 40.0 16.5 41.0 20-21 32.75266899094399 37.0 33.0 40.0 4.5 41.0 22-23 31.845727447331278 37.0 32.0 40.0 2.0 41.0 24-25 31.152730484061905 36.5 31.0 40.0 2.0 41.0 26-27 30.092857317003798 35.5 28.5 39.0 2.0 40.5 28-29 30.03243761970164 35.0 28.5 39.5 2.0 40.5 30-31 30.273194012141502 35.0 29.5 40.0 2.0 41.0 32-33 30.059788512435425 35.0 29.0 40.0 2.0 41.0 34-35 29.74850669245585 35.0 28.0 40.0 2.0 41.0 36-37 29.433924696665557 35.0 27.5 39.0 2.0 41.0 38-39 29.1183031421087 35.0 27.0 39.0 2.0 41.0 40-41 28.70752369652198 35.0 26.0 39.0 2.0 41.0 42-43 28.32520174889678 35.0 26.0 38.0 2.0 40.0 44-45 27.92570439418443 35.0 24.5 37.5 2.0 40.0 46-47 27.524942638027667 35.0 23.5 37.0 2.0 40.0 48-49 27.047722181136514 34.0 21.0 36.5 2.0 39.0 50-51 26.585214697667865 34.0 19.0 36.0 2.0 39.0 52-53 26.11341349110246 34.0 12.5 35.5 2.0 39.0 54-55 25.612865606384446 33.5 4.5 35.0 2.0 37.5 56-57 25.10045185251404 33.0 2.0 35.0 2.0 37.0 58-59 24.57960514375032 33.0 2.0 35.0 2.0 36.5 60-61 24.13749739263762 33.0 2.0 35.0 2.0 36.0 62-63 23.68136270916464 33.0 2.0 35.0 2.0 36.0 64-65 23.21180654319282 32.0 2.0 35.0 2.0 35.0 66-67 22.705458123728487 32.0 2.0 35.0 2.0 35.0 68-69 22.2338865004998 31.0 2.0 35.0 2.0 35.0 70-71 21.700766225920837 31.0 2.0 35.0 2.0 35.0 72-73 21.15277721341363 31.0 2.0 35.0 2.0 35.0 74-75 20.555464354308718 30.0 2.0 34.0 2.0 35.0 76-77 19.263389652360715 27.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9950469164990494 1101 2 0.8074377434647175 1101 3 0.9539226638826648 1101 4 1.1082338074998788 1101 5 1.1780305992039217 1101 6 1.19829378916274 1101 7 1.0216417309522896 1101 8 1.4212332326300015 1101 9 1.3044927643306181 1101 10-11 1.3217271693389492 1101 12-13 1.5298094057456595 1101 14-15 1.5288068192733988 1101 16-17 1.402377882305018 1101 18-19 1.556357856952232 1101 20-21 1.392099307976629 1101 22-23 1.6323443355596403 1101 24-25 1.5801902760957738 1101 26-27 1.5194070039215894 1101 28-29 1.6067747479161945 1101 30-31 1.5448227398051024 1101 32-33 1.371393851791229 1101 34-35 1.29936397077417 1101 36-37 1.090465940727725 1101 38-39 1.3350828050346557 1101 40-41 1.65522330000152 1101 42-43 1.6266589928473962 1101 44-45 1.7818904470165826 1101 46-47 1.3949597206732172 1101 48-49 1.4151143573078144 1101 50-51 1.4049571825353517 1101 52-53 1.5636271576798748 1101 54-55 1.7605996639202282 1101 56-57 1.6858308724276014 1101 58-59 1.5661899764451555 1101 60-61 1.4388916295624448 1101 62-63 1.4179737229091032 1101 64-65 1.3303236211154967 1101 66-67 1.295378275170318 1101 68-69 1.2434957878397022 1101 70-71 1.0398610835149285 1101 72-73 1.0896336715239556 1101 74-75 1.1889606736304579 1101 76-77 1.2074284212209783 1102 1 0.6361430335316385 1102 2 0.7452653614544573 1102 3 0.8684879275132289 1102 4 1.0522321058951718 1102 5 1.0461302376129211 1102 6 0.927107628881032 1102 7 0.909810851553523 1102 8 0.7818576270241593 1102 9 1.0590953687311568 1102 10-11 0.8695139015489062 1102 12-13 0.9040005526744963 1102 14-15 0.9994588647440565 1102 16-17 1.106784329496186 1102 18-19 1.4298931770666172 1102 20-21 1.4162810015459897 1102 22-23 1.0690150986820832 1102 24-25 1.6271923794682586 1102 26-27 1.39086494828857 1102 28-29 1.408913570500271 1102 30-31 1.194821558135164 1102 32-33 1.174573725588882 1102 34-35 1.4449409802038922 1102 36-37 1.3912009394278897 1102 38-39 1.438191778636149 1102 40-41 1.6410952542472579 1102 42-43 1.6095011453773544 1102 44-45 1.4289989006833075 1102 46-47 1.321212881640296 1102 48-49 1.28245063693819 1102 50-51 1.3369603494107771 1102 52-53 1.2178906228087918 1102 54-55 0.8474287708140906 1102 56-57 1.0281901946217253 1102 58-59 0.9087761792669831 1102 60-61 0.7639796639726733 1102 62-63 0.9826937853012794 1102 64-65 1.2088172282811414 1102 66-67 1.0112563740997267 1102 68-69 0.9735397459613182 1102 70-71 0.9922634184947228 1102 72-73 1.0170023289105714 1102 74-75 0.9460400582167559 1102 76-77 1.1196031665711068 1103 1 0.6245518898971483 1103 2 0.3442755875544705 1103 3 0.2453290946020772 1103 4 0.4920529146014303 1103 5 0.44908079582663873 1103 6 0.5846458594842758 1103 7 0.4228561653728278 1103 8 0.4177287218735799 1103 9 0.5978654222070929 1103 10-11 0.7894289967736015 1103 12-13 0.7790286979038754 1103 14-15 0.8295296969046717 1103 16-17 0.743668652603418 1103 18-19 0.9435600313681434 1103 20-21 0.5195223937966702 1103 22-23 0.7239358229526509 1103 24-25 0.5342615229151129 1103 26-27 0.4754155627927439 1103 28-29 0.7072952198107103 1103 30-31 0.9037459412855533 1103 32-33 0.7984526072850997 1103 34-35 0.9701192333233273 1103 36-37 0.8425073471584454 1103 38-39 0.8429881948102746 1103 40-41 0.9538989881810203 1103 42-43 0.9324535799396969 1103 44-45 0.8442371969587548 1103 46-47 0.7898590961069445 1103 48-49 0.6694921042428135 1103 50-51 0.7833460328707673 1103 52-53 1.0528071252949545 1103 54-55 0.9860276074779435 1103 56-57 0.8226455844331504 1103 58-59 0.616265155718807 1103 60-61 0.9207040283595767 1103 62-63 0.9518043960527578 1103 64-65 1.0697117774883864 1103 66-67 1.0428048308029823 1103 68-69 0.9142799654941065 1103 70-71 1.0126288373895527 1103 72-73 1.0322429706218017 1103 74-75 0.9974848466584483 1103 76-77 0.8921381135642577 1104 1 0.37712855474926243 1104 2 0.2625481333719222 1104 3 0.43903259668881134 1104 4 0.13523019640146572 1104 5 0.2895906851389185 1104 6 0.3335934189108798 1104 7 0.5795518648829017 1104 8 0.6912725433215954 1104 9 0.20483330462407423 1104 10-11 0.39444623502436826 1104 12-13 0.5059475873976211 1104 14-15 0.3902446306735001 1104 16-17 0.36817872337098123 1104 18-19 0.5875538618889209 1104 20-21 0.09368316316702163 1104 22-23 0.36119312652732205 1104 24-25 0.5054691077454514 1104 26-27 0.32496192461455564 1104 28-29 0.41110578050750135 1104 30-31 0.45172725139261516 1104 32-33 0.26425736141320755 1104 34-35 0.4149568308555338 1104 36-37 0.324153146468916 1104 38-39 0.1308397643983703 1104 40-41 0.28041413969617324 1104 42-43 0.06579598603659065 1104 44-45 0.12738000225724733 1104 46-47 0.010449733014947782 1104 48-49 -0.11705298739209624 1104 50-51 -0.3336427169024141 1104 52-53 -0.23078478179994732 1104 54-55 -0.2820135828686361 1104 56-57 -0.25148343026472375 1104 58-59 -0.4048743833848043 1104 60-61 -0.37773999268923575 1104 62-63 -0.3162277215302609 1104 64-65 -0.5499307556272015 1104 66-67 -0.3561427286236203 1104 68-69 -0.3462262947127499 1104 70-71 -0.35090318946582855 1104 72-73 -0.044771182889356 1104 74-75 -0.1392916004473399 1104 76-77 -0.2999640457534696 1105 1 0.09987782365331555 1105 2 0.172649442381811 1105 3 0.4515482630305456 1105 4 0.4575303635091075 1105 5 0.4993891115419302 1105 6 0.3950228855589799 1105 7 0.3950284698125728 1105 8 0.7804401383640709 1105 9 0.6764859296066703 1105 10-11 0.6797346697827606 1105 12-13 0.6591687613288286 1105 14-15 0.48352899218303946 1105 16-17 0.5356083989036264 1105 18-19 0.8616669728762503 1105 20-21 0.6828768687790472 1105 22-23 0.5780892760886651 1105 24-25 0.7354727632251858 1105 26-27 0.6741646818906979 1105 28-29 0.7392851775274139 1105 30-31 0.7437609166202996 1105 32-33 1.112480972330907 1105 34-35 0.9977795574952744 1105 36-37 0.9000478686524573 1105 38-39 0.8131872786721495 1105 40-41 1.1786699536496634 1105 42-43 1.1094223960073464 1105 44-45 1.0288126021736907 1105 46-47 0.9315609988553568 1105 48-49 1.1237909062160334 1105 50-51 1.0751051942520213 1105 52-53 1.1439584642660634 1105 54-55 1.1808963530734324 1105 56-57 0.9373786711139154 1105 58-59 1.0204450359161292 1105 60-61 0.8000468754349797 1105 62-63 0.8799888569407059 1105 64-65 0.8644439832671011 1105 66-67 1.1107708279840942 1105 68-69 0.9316293312446362 1105 70-71 0.589390641476939 1105 72-73 0.7912413849981235 1105 74-75 0.9690933737902299 1105 76-77 1.175735717510701 1106 1 -0.11346662376977434 1106 2 0.0783905120152113 1106 3 -0.2807166655935376 1106 4 -0.08038180725692712 1106 5 -0.28368926792325766 1106 6 -0.026287683437736575 1106 7 -0.06617972668900052 1106 8 0.23702397992964563 1106 9 0.2342945550339195 1106 10-11 0.02378625814815649 1106 12-13 0.13021418388763806 1106 14-15 0.13229583086677366 1106 16-17 0.3821543743744442 1106 18-19 0.30350021126509574 1106 20-21 0.19969879760419218 1106 22-23 -0.08146981847604806 1106 24-25 -0.08946566248354415 1106 26-27 0.3340065155479621 1106 28-29 0.4783588823729339 1106 30-31 0.18882366375520832 1106 32-33 0.20467695450368595 1106 34-35 0.2563496935451397 1106 36-37 0.35892294404954583 1106 38-39 0.2028017482117228 1106 40-41 0.13488099848579793 1106 42-43 0.36373469075971343 1106 44-45 -0.011537407879252726 1106 46-47 0.19314701855913086 1106 48-49 0.25738617462967994 1106 50-51 0.3146773576458948 1106 52-53 0.34460478488800206 1106 54-55 0.6152150146912874 1106 56-57 0.5022892836733952 1106 58-59 0.20233842442896233 1106 60-61 0.13913691631696068 1106 62-63 3.3727527727833717E-4 1106 64-65 -0.014099680026241401 1106 66-67 0.11721099620338649 1106 68-69 0.16813115752973928 1106 70-71 -0.17064554912690966 1106 72-73 0.07040121007687361 1106 74-75 0.007667363469206379 1106 76-77 0.1572634301304987 1107 1 0.2043442890265652 1107 2 0.1774917268479541 1107 3 -0.06799754192898178 1107 4 0.12484213274976241 1107 5 0.11024771362338726 1107 6 -0.037763315134839104 1107 7 -0.16363303603350232 1107 8 0.1232786967605719 1107 9 -0.3929683296763855 1107 10-11 -0.23790232751905194 1107 12-13 -0.14133795202078403 1107 14-15 -0.2948341068790228 1107 16-17 -0.5534559079189307 1107 18-19 -0.6151374405889953 1107 20-21 -0.9413682809061612 1107 22-23 -0.7249550191702347 1107 24-25 -1.1637587349117773 1107 26-27 -1.1910543853070372 1107 28-29 -1.0625063874218341 1107 30-31 -0.5860163222336148 1107 32-33 -0.5837841246753896 1107 34-35 -0.7063833616875606 1107 36-37 -0.8944882999200736 1107 38-39 -0.8865991147494121 1107 40-41 -0.7900278492541055 1107 42-43 -0.722129544118566 1107 44-45 -0.5630623175230802 1107 46-47 -0.5998696834008435 1107 48-49 -0.5097854782115654 1107 50-51 -0.7832118107588997 1107 52-53 -0.3892027524884014 1107 54-55 -0.3508675875664231 1107 56-57 -0.5953729991931667 1107 58-59 -0.5556543152338129 1107 60-61 -0.5459877513156322 1107 62-63 -0.6426205160517142 1107 64-65 -0.5046938813095423 1107 66-67 -0.4557804697160215 1107 68-69 -0.6736281208946409 1107 70-71 -0.7901545210567917 1107 72-73 -0.5299996210406803 1107 74-75 -0.6398540115367659 1107 76-77 -0.09694191322294898 1108 1 0.09945270866983691 1108 2 0.12657912431482998 1108 3 0.07277486146288226 1108 4 0.050071822341784866 1108 5 -0.01075291734969852 1108 6 -0.02830256627751737 1108 7 -0.05259331550840329 1108 8 0.12354031974646063 1108 9 -0.046918067283918674 1108 10-11 -0.03146447836778776 1108 12-13 0.12369190377878425 1108 14-15 0.28258321256264907 1108 16-17 0.16933337785031455 1108 18-19 0.1996354091009067 1108 20-21 -0.14947397659246064 1108 22-23 -0.0196406098995503 1108 24-25 0.27999039942393367 1108 26-27 0.1834281398164599 1108 28-29 0.08620127197581695 1108 30-31 0.16906416530851587 1108 32-33 0.31431949341660825 1108 34-35 0.3818162874298494 1108 36-37 0.3127171049030224 1108 38-39 0.22539091279795187 1108 40-41 0.25046985000258104 1108 42-43 0.31410716312531406 1108 44-45 0.2063689602743608 1108 46-47 0.18532630299476338 1108 48-49 0.310525699245769 1108 50-51 0.5929539504870398 1108 52-53 0.2780012672718257 1108 54-55 0.26736676507058377 1108 56-57 0.005957815377694686 1108 58-59 0.016503612712135407 1108 60-61 0.04467423178660468 1108 62-63 0.12944430489043413 1108 64-65 0.2773318500805999 1108 66-67 0.18437442648121305 1108 68-69 0.053023951313303286 1108 70-71 0.05760013814079912 1108 72-73 0.051247781966445416 1108 74-75 0.16456934062316364 1108 76-77 0.3768515431244843 1109 1 -0.41303632097873333 1109 2 -0.1861312202495995 1109 3 -0.7037161523883739 1109 4 -0.43586323317773434 1109 5 -0.5625215287893894 1109 6 -0.1867945722301272 1109 7 -0.4262721036184516 1109 8 -0.3235539680392421 1109 9 -0.05113831378294975 1109 10-11 -0.3512206503828992 1109 12-13 -0.3252988238893977 1109 14-15 -0.006595251206576336 1109 16-17 0.17467736991350336 1109 18-19 -0.27268114754426875 1109 20-21 -0.4017519939160792 1109 22-23 -0.4707300134940091 1109 24-25 -0.3447339108748402 1109 26-27 -0.4737645949490563 1109 28-29 -0.5046599483088414 1109 30-31 -0.2608763086106656 1109 32-33 -0.18082302575765752 1109 34-35 0.03015451767367594 1109 36-37 0.06130145259638908 1109 38-39 0.04366274856701935 1109 40-41 0.15507167394275 1109 42-43 -0.08790756576391345 1109 44-45 -0.13422897157590796 1109 46-47 -0.15708984536254533 1109 48-49 0.1443468157413612 1109 50-51 0.005710084344407562 1109 52-53 0.09908151300769674 1109 54-55 0.15681424093580887 1109 56-57 0.15359203524261744 1109 58-59 0.387072544676883 1109 60-61 0.3648405995509414 1109 62-63 0.3151294271860081 1109 64-65 0.31791642672042997 1109 66-67 0.49266992005163246 1109 68-69 0.24454148479672355 1109 70-71 0.11575254017078862 1109 72-73 0.10104745380984781 1109 74-75 0.24497343113333514 1109 76-77 -0.02116616206878419 1110 1 -0.38907643580014195 1110 2 -0.5045026457065234 1110 3 -0.5061579241263203 1110 4 -0.2048323840080073 1110 5 -0.24466970654455622 1110 6 -0.4024371724263389 1110 7 -0.39258873455482757 1110 8 -0.14587147103695486 1110 9 -0.2514835047280428 1110 10-11 -0.2632496464222882 1110 12-13 -0.17311140337415054 1110 14-15 -0.21052134400843414 1110 16-17 -0.29989295819957107 1110 18-19 -0.6377296559560293 1110 20-21 -0.8510507112591981 1110 22-23 -1.0135209731248338 1110 24-25 -0.9191425442820602 1110 26-27 -0.8606727741576599 1110 28-29 -0.9283691158536236 1110 30-31 -1.106890261592028 1110 32-33 -0.8612270808428626 1110 34-35 -0.4830899138513409 1110 36-37 -0.7391400284544467 1110 38-39 -0.6585410230982554 1110 40-41 -0.7288696799324761 1110 42-43 -0.6095165189269558 1110 44-45 -0.5348902847549333 1110 46-47 -0.5486144992632056 1110 48-49 -0.41948989077366505 1110 50-51 -0.46614958645244187 1110 52-53 -0.5758903266783584 1110 54-55 -0.5112937493261711 1110 56-57 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2314 3 -0.2523034811555007 2314 4 -0.20108155509554138 2314 5 -0.09937023231189102 2314 6 -0.07577757125165618 2314 7 -0.0019814969648308534 2314 8 -0.1944871872173266 2314 9 0.07097824688241872 2314 10-11 -0.2742208950012923 2314 12-13 -0.6220163507974164 2314 14-15 -0.628117083398358 2314 16-17 -1.0383118668771019 2314 18-19 -0.6702319058613 2314 20-21 -0.46327657806480715 2314 22-23 -0.426686471419643 2314 24-25 -0.43407925089441335 2314 26-27 -0.710789117943186 2314 28-29 -0.5780943905789009 2314 30-31 -0.6227387719179092 2314 32-33 -0.7276093515893614 2314 34-35 -0.6984413727372818 2314 36-37 -0.7743318032744533 2314 38-39 -0.7715173517265228 2314 40-41 -0.4556349674466844 2314 42-43 -0.39718318954082577 2314 44-45 -0.25608409633128915 2314 46-47 -0.3176084363605902 2314 48-49 -0.24921054727233738 2314 50-51 -0.018138680332697277 2314 52-53 0.08382038287071225 2314 54-55 -0.226885000747167 2314 56-57 -0.3554083928395748 2314 58-59 -0.5501140380041392 2314 60-61 -0.6729208363590402 2314 62-63 -0.8306631364147776 2314 64-65 -0.5192120590917 2314 66-67 -0.42277620274680316 2314 68-69 -0.40663115463576105 2314 70-71 -0.24519719074897495 2314 72-73 -0.09375134756001557 2314 74-75 0.019731870467971646 2314 76-77 -0.054642189466044044 2315 1 -1.2125437859625663 2315 2 -1.3018769490475108 2315 3 -1.1455970204813468 2315 4 -1.0725878753202593 2315 5 -0.9927816488447974 2315 6 -1.2650306812034984 2315 7 -1.4517607907048031 2315 8 -1.2795644344083357 2315 9 -1.1152602161994452 2315 10-11 -1.4051147553544254 2315 12-13 -1.3086372877958112 2315 14-15 -1.6937508614882475 2315 16-17 -1.5073527978562353 2315 18-19 -1.3846405134054365 2315 20-21 -1.522323026700441 2315 22-23 -1.5084782049669947 2315 24-25 -1.390270312290884 2315 26-27 -1.386339629580423 2315 28-29 -1.1795791417827495 2315 30-31 -1.369449496235724 2315 32-33 -1.0871875016696144 2315 34-35 -1.1058851929620026 2315 36-37 -1.359169534012814 2315 38-39 -1.3950012502016413 2315 40-41 -1.2139678827757372 2315 42-43 -1.3438487693963594 2315 44-45 -1.1726082235383508 2315 46-47 -1.050794632187234 2315 48-49 -1.0328657439015494 2315 50-51 -1.147152775437032 2315 52-53 -1.2758530721854697 2315 54-55 -1.1407769550007778 2315 56-57 -1.2265372551991227 2315 58-59 -1.3907402920169787 2315 60-61 -1.2899616168566332 2315 62-63 -1.2287369726908608 2315 64-65 -1.322686928674365 2315 66-67 -1.2167882914305892 2315 68-69 -1.2736669190819896 2315 70-71 -1.4587279893043537 2315 72-73 -2.5365006453128274 2315 74-75 -3.0369720821805046 2315 76-77 -2.9451618523873933 2316 1 -2.7294048752061073 2316 2 -2.701941358980953 2316 3 -2.1154603282892595 2316 4 -2.204714118789809 2316 5 -2.375827523030729 2316 6 -2.289857132216163 2316 7 -2.5645497408228835 2316 8 -2.5636551808479027 2316 9 -2.4352845488409685 2316 10-11 -2.5046889341277705 2316 12-13 -2.5541357491140744 2316 14-15 -2.6916969002773357 2316 16-17 -3.700743623019047 2316 18-19 -3.3456939736501923 2316 20-21 -2.7695820261994726 2316 22-23 -2.68713346868989 2316 24-25 -2.2034242520318124 2316 26-27 -2.186826282410884 2316 28-29 -2.595860541625303 2316 30-31 -2.558843327195433 2316 32-33 -2.7224555185593324 2316 34-35 -2.5526611179602092 2316 36-37 -2.4591766906720878 2316 38-39 -2.663443961144175 2316 40-41 -2.792834267947139 2316 42-43 -2.7555892689311783 2316 44-45 -2.338020089279425 2316 46-47 -2.050934187043026 2316 48-49 -2.2019275058710654 2316 50-51 -2.7598164840178647 2316 52-53 -3.7111139324159126 2316 54-55 -2.59246592658884 2316 56-57 -2.494754531374607 2316 58-59 -2.540446868986848 2316 60-61 -2.4383048502352906 2316 62-63 -2.3393073584347945 2316 64-65 -2.4284015211026215 2316 66-67 -2.4723418590252386 2316 68-69 -2.473502315918747 2316 70-71 -2.1713559145888297 2316 72-73 -2.1738369367319876 2316 74-75 -1.9355300153373065 2316 76-77 -1.6128030174915224 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17918.0 3 3010.0 4 5591.0 5 6933.0 6 7513.0 7 7421.0 8 10087.0 9 11277.0 10 16826.0 11 19545.0 12 12031.0 13 7295.0 14 6630.0 15 6264.0 16 6140.0 17 6342.0 18 6662.0 19 6892.0 20 6967.0 21 7391.0 22 7987.0 23 8797.0 24 10030.0 25 10819.0 26 12312.0 27 13849.0 28 15618.0 29 17424.0 30 19747.0 31 22894.0 32 27518.0 33 34070.0 34 50515.0 35 62277.0 36 66399.0 37 81123.0 38 74206.0 39 23730.0 40 244.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.34980690378241 26.725437632834076 39.02304556962368 16.90170989375983 2 17.39404308582622 27.502265643097207 38.80135627017574 16.30233500090084 3 17.24738180355162 27.654196226220844 38.35889780397969 16.739524166247854 4 17.471860111326805 27.607909648910102 38.37035873024044 16.549871509522653 5 17.249698595250674 27.753484780753446 38.36591213882229 16.630904485173595 6 17.057919932378432 28.03260790056162 38.657538477376846 16.251933689683103 7 17.452782902954 28.036429842885124 37.90318429230573 16.607602961855143 8 16.716565108661197 28.368551247280422 37.7331115223405 17.181772121717877 9 16.709317243070355 28.340800006502576 37.97815947296622 16.97172327746085 10-11 17.087721009500818 28.390821882362182 37.877143712566735 16.644313395570258 12-13 17.057451302066045 28.613211266193694 37.09719808229236 17.232139349447895 14-15 17.074688852887153 28.924828916498246 36.962637964011506 17.03784426660309 16-17 17.050866651134314 29.252794469037337 36.62860788573967 17.067730994088677 18-19 16.914768451504834 29.421211737380816 36.12608993980396 17.537929871310393 20-21 16.86879853467943 29.607644703115433 35.61822275690527 17.905334005299864 22-23 17.336648867459694 30.163525557091695 34.269242414902834 18.230583160545777 24-25 17.37499491986941 30.790332850156467 32.98183345299863 18.852838776975496 26-27 17.72460500895521 31.299128323655566 32.18767188986043 18.788594777528797 28-29 17.784565785730734 31.5185646749658 31.733131950747058 18.963737588556413 30-31 17.460244355236895 31.393667407855425 31.783316412194868 19.36277182471281 32-33 17.695331099565234 31.556004386259005 31.465989898599933 19.282674615575825 34-35 17.55087886133762 31.06070919852967 31.899980967204666 19.48843097292805 36-37 17.696251744083664 31.29861421546714 31.955473374784955 19.04966066566424 38-39 18.161744635052145 31.067218734357947 31.952351311205923 18.818685319383984 40-41 17.85324104709971 31.27502250374385 32.040657779860894 18.831078669295547 42-43 17.81916136596547 31.283540721644947 31.70572286075982 19.191575051629766 44-45 18.164540200339612 31.141640471066985 31.60398349220025 19.089835836393153 46-47 17.804160332956698 31.37089934617146 31.547701303592667 19.277239017279175 48-49 17.861732185109442 31.314661260178656 31.167260851982487 19.656345702729414 50-51 17.462339133427456 31.486760772538936 31.297406352552866 19.753493741480746 52-53 17.078957548706963 31.464186485151636 31.65591817460592 19.800937791535485 54-55 17.191725513361767 31.285531571274504 31.466474997154766 20.056267918208963 56-57 17.181340074620195 31.439649496435624 31.16429448151972 20.214715947424466 58-59 17.27057083134079 31.0382175355508 30.789358534427752 20.901853098680654 60-61 17.54572777318293 30.404574707831994 30.915788282933377 21.1339092360517 62-63 17.501774808022674 30.413977358326967 30.744755510033762 21.3394923236166 64-65 17.678869369592125 30.05758693079475 30.873463879967726 21.3900798196454 66-67 17.8746301254051 30.17736367479217 30.587612859171266 21.36039334063147 68-69 18.211900338627522 29.674353877278353 30.745793579492098 21.367952204602027 70-71 18.341596687340296 29.307768772449794 30.854733692326768 21.49590084788314 72-73 18.592745474817924 29.01857519037791 30.776524971955904 21.612154362848262 74-75 18.678494956731804 28.626554140658417 30.73204490104946 21.962906001560327 76-77 19.108326882641695 28.385257446990224 31.159348577300523 21.34706709306755 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 46601.0 1 27002.0 2 6618.0 3 5635.0 4 5437.0 5 5377.5 6 5210.0 7 5098.5 8 4989.5 9 4884.0 10 4800.0 11 4720.0 12 4818.0 13 4912.0 14 5152.0 15 5521.5 16 5939.5 17 6228.0 18 6542.5 19 7054.5 20 7629.0 21 8650.0 22 9294.0 23 9762.0 24 10687.0 25 11831.0 26 12518.0 27 13276.5 28 14471.5 29 15159.5 30 15411.0 31 16097.5 32 17871.0 33 19555.5 34 20484.0 35 20815.0 36 21155.0 37 21882.5 38 22433.0 39 22596.0 40 22757.0 41 22971.5 42 23049.0 43 23073.0 44 23063.0 45 23170.5 46 23031.0 47 22810.0 48 22846.0 49 22832.0 50 22933.5 51 22226.5 52 21404.0 53 20766.0 54 19350.0 55 17631.5 56 16076.5 57 15462.0 58 15255.0 59 13760.5 60 11918.0 61 11363.0 62 10456.5 63 7428.0 64 4419.0 65 3532.0 66 3101.5 67 2400.5 68 1920.5 69 1446.0 70 1181.0 71 1027.5 72 853.5 73 763.5 74 694.0 75 699.0 76 505.0 77 246.5 78 187.0 79 159.0 80 124.5 81 94.0 82 59.0 83 48.0 84 45.5 85 38.0 86 27.5 87 22.0 88 19.5 89 17.0 90 19.5 91 22.0 92 15.0 93 10.5 94 11.5 95 10.0 96 10.0 97 9.0 98 7.0 99 19.0 100 32.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008262291173976762 2 0.012596607855407194 3 0.012867502647996597 4 0.01449287140353301 5 0.011377581288754886 6 0.009210422948039669 7 0.01462831879982771 8 0.01733726672572173 9 0.016930924536837626 10-11 0.019910767255321052 12-13 0.016389134951658824 14-15 0.007855948985092658 16-17 0.0067723698147350506 18-19 0.016592306046100878 20-21 0.021942478199741565 22-23 0.020655727934941907 24-25 0.016795477140542928 26-27 0.023838741747867383 28-29 0.008668633362860865 30-31 0.008871804457302917 32-33 0.01076806800542873 34-35 0.012799778949849246 36-37 0.011377581288754886 38-39 0.013477015931322753 40-41 0.011309857590607536 42-43 0.01428970030909096 44-45 0.013206121138733349 46-47 0.024380531333046185 48-49 0.022822886275657123 50-51 0.03521632303662227 52-53 0.038399336849547744 54-55 0.029527532392244822 56-57 0.021129793821973362 58-59 0.016930924536837626 60-61 0.020113938349763102 62-63 0.025193215710814388 64-65 0.02607362378672995 66-67 0.02790216363670841 68-69 0.04266592983283082 70-71 0.053840340027143656 72-73 0.08350331981568318 74-75 0.08499324117492489 76-77 0.08763446540267156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 738294.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.65160239044707 #Duplication Level Percentage of deduplicated Percentage of total 1 83.5635436413876 49.84699279632824 2 8.808100421627405 10.50834608332094 3 3.054299192105384 5.465815229668022 4 1.530400191122411 3.6516329479639302 5 0.8659485258040732 2.5827608575929184 6 0.5224902097046791 1.8700426945322892 7 0.33251036983346133 1.3884343460404298 8 0.23583491154181413 1.1254344298462842 9 0.17120093525177146 0.919116910666021 >10 0.8372729155778185 8.763751152381014 >50 0.0497662685731015 2.007690614291837 >100 0.02704172761118786 3.0344926246478954 >500 9.089656338866833E-4 0.40706608376826225 >1k 2.2724140847167084E-4 0.18841886661268215 >5k 0.0 0.0 >10k+ 4.544828169433417E-4 8.240004362339368 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36990 5.010199188940991 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23798 3.2233771370212954 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 1390 0.18827188084963442 No Hit GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC 949 0.12853957908367128 No Hit AGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG 746 0.10104375763584697 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 9.481317740629071E-4 0.0 2 0.0 0.0 0.0 0.006366027625850948 0.0 3 0.0 0.0 0.0 0.009887659929513175 0.0 4 0.0 0.0 0.0 0.02072345163308926 0.0 5 0.0 0.0 0.0 0.03697713918845338 0.0 6 0.0 0.0 0.0 0.061086775728910164 0.0 7 0.0 0.0 0.0 0.10740978526169792 0.0 8 0.0 0.0 0.0 0.15332645260560157 0.0 9 0.0 0.0 0.0 0.20520280538647206 0.0 10 0.0 0.0 0.0 0.26642502851167693 0.0 11 0.0 0.0 0.0 0.332523357903491 0.0 12 0.0 0.0 0.0 0.4110828477544176 0.0 13 0.0 0.0 0.0 0.5176799486383473 0.0 14 0.0 0.0 0.0 0.6510956339886278 0.0 15 0.0 0.0 0.0 0.7995459803276201 0.0 16 0.0 0.0 0.0 0.9666880673552812 0.0 17 0.0 0.0 0.0 1.1075533595017704 0.0 18 0.0 0.0 0.0 1.2416462818335243 0.0 19 0.0 0.0 0.0 1.3751974145800996 0.0 20 0.0 0.0 0.0 1.5216160499746714 0.0 21 0.0 0.0 0.0 1.6720981072580843 0.0 22 0.0 0.0 0.0 1.8308424557154739 0.0 23 0.0 0.0 0.0 1.9994744641023765 0.0 24 0.0 0.0 0.0 2.179754948570624 0.0 25 0.0 0.0 0.0 2.378185384142361 0.0 26 0.0 0.0 0.0 2.5829818473399486 0.0 27 0.0 0.0 0.0 2.7887264423115994 0.0 28 0.0 0.0 0.0 2.979165481501949 0.0 29 0.0 0.0 0.0 3.152673596155461 0.0 30 0.0 0.0 0.0 3.341622713986569 0.0 31 0.0 0.0 0.0 3.5366669646509385 0.0 32 0.0 0.0 0.0 3.7505384034002716 0.0 33 0.0 0.0 0.0 3.959533735882995 0.0 34 0.0 0.0 0.0 4.197785705965374 0.0 35 0.0 0.0 0.0 4.43305783332927 0.0 36 0.0 0.0 0.0 4.699482861840947 0.0 37 0.0 0.0 0.0 4.984057841456114 0.0 38 0.0 0.0 0.0 5.2557653184232835 0.0 39 0.0 0.0 0.0 5.496048999450084 0.0 40 0.0 0.0 0.0 5.7384998388175985 0.0 41 0.0 0.0 0.0 6.006143893895928 0.0 42 0.0 0.0 0.0 6.280424871392698 0.0 43 0.0 0.0 0.0 6.578002801052155 0.0 44 0.0 0.0 0.0 6.880998626563402 0.0 45 0.0 0.0 0.0 7.208104088615104 0.0 46 0.0 0.0 0.0 7.562840819510926 0.0 47 0.0 0.0 0.0 7.923537235843716 0.0 48 0.0 0.0 0.0 8.273533307869222 0.0 49 0.0 0.0 0.0 8.599419743354273 0.0 50 0.0 0.0 0.0 8.918940151213473 0.0 51 0.0 0.0 0.0 9.270155249805633 0.0 52 0.0 0.0 0.0 9.614597978583058 0.0 53 0.0 0.0 0.0 9.96472949800486 0.0 54 0.0 0.0 0.0 10.354682551937303 0.0 55 0.0 0.0 0.0 10.736644209488361 0.0 56 0.0 0.0 0.0 11.148675189016842 0.0 57 0.0 0.0 0.0 11.566936748774879 0.0 58 0.0 0.0 0.0 12.006463549751183 0.0 59 0.0 0.0 0.0 12.426621373057346 0.0 60 0.0 0.0 0.0 12.82551395514524 0.0 61 0.0 0.0 0.0 13.22833451172568 0.0 62 0.0 0.0 0.0 13.632915884457953 0.0 63 0.0 0.0 0.0 14.0509065494234 0.0 64 0.0 0.0 0.0 14.480003900885013 0.0 65 0.0 0.0 0.0 14.922781439372391 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGCGA 20 0.0065706987 53.252384 60 CGGTACA 20 0.0065777474 53.237953 9 CGCAATT 20 0.006581274 53.23074 2 TAACGGG 30 5.4871937E-4 47.322628 11 GTTAGCG 40 0.0022580323 35.494373 36 CGGTTAC 90 3.938112E-9 35.48476 4 TCGCAAT 45 0.004025714 31.544144 1 ATCACCG 50 0.0067271446 28.39935 16 CGCGCTT 50 0.006736055 28.391655 38 GGCGGTT 145 5.0567905E-10 26.921295 2 GCGGTTA 125 9.620999E-8 25.549025 3 GCCTAGT 120 1.9161143E-6 23.666124 17 TTAGTCG 75 0.0016874765 23.666124 15 ATGGCGG 75 0.0016908074 23.658108 6 GGGCGGT 180 2.401066E-10 23.658108 1 GGCGACC 75 0.0016908074 23.658108 6 GTGGGTA 215 3.6379788E-12 23.10792 1 CGTGTGC 80 0.002455783 22.18699 55 CGAACTC 80 0.0024596525 22.180979 35 TAGTCGG 85 0.003490958 20.881874 16 >>END_MODULE