FastQCFastQC Report
Thu 26 May 2016
SRR936181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936181_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738294
Sequences flagged as poor quality0
Sequence length125
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209552.83830018935546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144021.9507134014362844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111701.5129474166118104No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50580.6850929304585979No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46780.6336229198666115No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35700.4835472047720827No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28620.3876504481954343No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25090.3398375173034049No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19630.2658832389264981No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19230.2604653430747101No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13990.18949090741628674No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG13860.1877300912644556No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT12120.16416224430917764No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11020.14926303071676053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7890.10686799567651911No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7680.10402360035433038No Hit
GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG7680.10402360035433038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7600.10294002118397277No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC7460.10104375763584697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11400.056.653187100-101
CCCGGTA1650.054.1099898-99
ACCGTAC1050.053.85230698-99
AACGTAT1400.053.14372698-99
AGGTATC17400.053.02515100-101
CGAGCGT850.052.51494696-97
CCCGTAC2450.052.23269398-99
AAGTATC18650.052.18412100-101
GTCGTAC800.052.08085398-99
AGTATCA27000.051.919033100-101
TACGCAG8550.051.86682100-101
TCCGGTA1100.051.40447698-99
CCGTACA4700.050.026337100-101
CCGTATC4600.049.819843100-101
ACGTATC2750.049.784554100-101
CGGTATC8650.049.20275100-101
CGAGTAC7100.048.62606100-101
CGTACAT11500.048.396416100-101
TGTATCA17450.048.268127100-101
TGAGTAC12600.048.186913100-101
Produced by FastQC (version 0.11.5)