FastQCFastQC Report
Thu 26 May 2016
SRR936180_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936180_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739353
Sequences flagged as poor quality0
Sequence length77
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT373925.057394776243553No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA242863.28476384081758No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG14010.18949000004057603No Hit
GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC9290.12565039974139552No Hit
AGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG8100.10955524627613604No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG252.2286622E-456.80005355
GGCGATT200.006563052553.26807464
ATCGCGT903.932655E-935.4904252
TACGTAG450.004023906331.5470472
TAAGGCG952.4925612E-729.89476422
TCGCGAT853.5558314E-629.22148328
CGACGGC500.00672636828.40002656
TGCGGAC851.1904448E-425.0520673
TGGCGAT750.001684588723.67309863
CGCGTCA1051.8302613E-523.6618864
TTCGCGA901.7552591E-423.65868427
GCGTCAA1102.6225494E-522.5863465
CGGTATA800.002459308122.1815191
CGGTTGC1003.577536E-421.2956984
CGAATGT1106.787145E-419.36627658
CGTCAAC1309.489542E-519.1128166
GCAGTAC1501.3216659E-518.93591349
AGGCGGA1701.8490337E-618.79413624
CGGTTAC950.00664949518.6804374
CGTGACC950.00665209218.67917326
Produced by FastQC (version 0.11.5)