##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936180_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 739353 Sequences flagged as poor quality 0 Sequence length 77 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.85105761388674 40.0 36.0 41.0 31.0 41.0 2 36.6639372532471 39.0 36.0 41.0 30.0 41.0 3 36.480687844642546 39.0 35.0 41.0 30.0 41.0 4 36.35666454318844 39.0 35.0 41.0 30.0 41.0 5 36.190344801468314 39.0 35.0 41.0 30.0 41.0 6 35.92943830619474 39.0 35.0 40.0 30.0 41.0 7 35.57159705850926 38.0 35.0 40.0 30.0 41.0 8 35.23987729812417 38.0 35.0 40.0 29.0 41.0 9 35.148742211095374 38.0 35.0 40.0 29.0 41.0 10-11 35.08173835772628 38.0 35.0 40.0 28.0 41.0 12-13 34.711745269174536 38.0 35.0 40.0 25.0 41.0 14-15 34.33778925628218 38.0 34.0 40.0 23.5 41.0 16-17 33.92227799170355 38.0 33.5 40.0 21.0 41.0 18-19 33.420386473037915 37.0 33.0 40.0 16.0 41.0 20-21 32.71707290022492 37.0 33.0 40.0 4.0 41.0 22-23 31.800474874653922 37.0 31.5 40.0 2.0 41.0 24-25 31.108462398881183 36.5 31.0 40.0 2.0 41.0 26-27 30.02582122477355 35.5 28.5 39.0 2.0 40.5 28-29 29.947931502272933 35.0 28.5 39.0 2.0 40.5 30-31 30.22388967110433 35.0 29.0 40.0 2.0 41.0 32-33 30.040989216247176 35.0 29.0 40.0 2.0 41.0 34-35 29.75885943520889 35.0 28.0 40.0 2.0 41.0 36-37 29.422352381068315 35.0 27.5 39.0 2.0 41.0 38-39 29.079479626105528 35.0 27.0 39.0 2.0 41.0 40-41 28.696097804431712 35.0 26.0 39.0 2.0 41.0 42-43 28.308794986968337 35.0 26.0 38.0 2.0 40.0 44-45 27.91426355205159 35.0 24.5 37.5 2.0 40.0 46-47 27.48553870749155 35.0 23.5 37.0 2.0 40.0 48-49 27.044897362964647 34.0 21.0 36.5 2.0 39.0 50-51 26.56844700704535 34.0 19.0 36.0 2.0 39.0 52-53 26.07498380340649 34.0 10.5 35.5 2.0 38.5 54-55 25.570737523212863 33.5 4.5 35.0 2.0 37.5 56-57 25.064164884703246 33.0 2.0 35.0 2.0 37.0 58-59 24.546956595834466 33.0 2.0 35.0 2.0 36.5 60-61 24.090560260119318 33.0 2.0 35.0 2.0 36.0 62-63 23.62434993839208 32.5 2.0 35.0 2.0 36.0 64-65 23.136344885325414 32.0 2.0 35.0 2.0 35.0 66-67 22.658730673981168 32.0 2.0 35.0 2.0 35.0 68-69 22.180887884407042 31.0 2.0 35.0 2.0 35.0 70-71 21.630020436787298 31.0 2.0 35.0 2.0 35.0 72-73 21.080868678425595 30.5 2.0 34.5 2.0 35.0 74-75 20.504065040650406 30.0 2.0 34.0 2.0 35.0 76-77 19.178183492864708 27.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7415924966349507 1101 2 0.4951409971754188 1101 3 0.46980949689014295 1101 4 0.5390547764747993 1101 5 0.5821245112300417 1101 6 0.6371698923437421 1101 7 0.49807482253467583 1101 8 0.5120212234209802 1101 9 0.25044930428876455 1101 10-11 0.2238546270781967 1101 12-13 0.5516297411577114 1101 14-15 0.6781977780910324 1101 16-17 0.9236859787652918 1101 18-19 0.9003229921753402 1101 20-21 1.1000891351510447 1101 22-23 0.8549871748231688 1101 24-25 0.8232430927507153 1101 26-27 0.7615626657011347 1101 28-29 0.5966149229731776 1101 30-31 0.3075921611860153 1101 32-33 0.19045179259942557 1101 34-35 0.3427369574882917 1101 36-37 0.3403798733333936 1101 38-39 0.5927246949513894 1101 40-41 0.5482095058085967 1101 42-43 0.401016708294172 1101 44-45 0.35444872562031904 1101 46-47 0.4229736071282453 1101 48-49 0.5367597416065983 1101 50-51 0.4101320737614138 1101 52-53 0.5494984749457323 1101 54-55 0.6675225602842687 1101 56-57 0.6536008984953128 1101 58-59 0.47567214840965377 1101 60-61 0.12284163396435233 1101 62-63 0.31248092320383236 1101 64-65 0.40986398901945975 1101 66-67 0.3845222037296594 1101 68-69 0.10518465084783557 1101 70-71 -0.20528974463056215 1101 72-73 0.040912131837938404 1101 74-75 0.1620716950362997 1101 76-77 0.37406272049759437 1102 1 0.8911981057497584 1102 2 0.8793702307911602 1102 3 0.694251993725274 1102 4 0.7551176842554952 1102 5 0.7894523912573064 1102 6 0.9549710414332324 1102 7 0.7553101911197331 1102 8 0.9725431827159454 1102 9 1.02086998789963 1102 10-11 0.9912467782126839 1102 12-13 1.0484697450529268 1102 14-15 1.0516786428295646 1102 16-17 1.311547503743384 1102 18-19 1.2544421858650878 1102 20-21 1.0222018043495993 1102 22-23 0.9142066703923568 1102 24-25 0.8895957074162126 1102 26-27 0.9334270147127235 1102 28-29 1.2585805476688599 1102 30-31 1.1422474343382198 1102 32-33 1.3069431744282305 1102 34-35 1.1471069058766474 1102 36-37 1.216804062641021 1102 38-39 1.2162542499226596 1102 40-41 1.2290206850860308 1102 42-43 1.2895355514142395 1102 44-45 1.0929529616705658 1102 46-47 1.1942244592073195 1102 48-49 1.2196451247987277 1102 50-51 1.0540798778319207 1102 52-53 0.8460755515841605 1102 54-55 0.8902706370200768 1102 56-57 0.8381490531354956 1102 58-59 0.9963484557423996 1102 60-61 0.8023698256091087 1102 62-63 0.7309442594585782 1102 64-65 0.8722313081352446 1102 66-67 0.8673102064563096 1102 68-69 0.9395210029755994 1102 70-71 0.9075936909522611 1102 72-73 1.1019891597861324 1102 74-75 0.6432139678963615 1102 76-77 0.8605950021219009 1103 1 0.3454511569427723 1103 2 0.4689998983484358 1103 3 0.25148688205766234 1103 4 0.5590104572802517 1103 5 0.16662965370180416 1103 6 0.21352415686189374 1103 7 0.3276241637683839 1103 8 0.1927736862675289 1103 9 0.3580582355699278 1103 10-11 0.3833146073272502 1103 12-13 0.34075588225653775 1103 14-15 0.26753949353290096 1103 16-17 0.18238964232480015 1103 18-19 0.002729235401012886 1103 20-21 0.17736230036047118 1103 22-23 0.044752812995486835 1103 24-25 0.09235574539902558 1103 26-27 0.044609369461038995 1103 28-29 -0.10948619223264089 1103 30-31 0.07915802577160846 1103 32-33 0.21832591645230437 1103 34-35 0.05618205226874551 1103 36-37 0.1213792229973869 1103 38-39 -0.06590430039991446 1103 40-41 0.11641048372125695 1103 42-43 -0.06459135587231657 1103 44-45 -0.1509562891928944 1103 46-47 -0.13449562234401924 1103 48-49 -0.12733207620297193 1103 50-51 -0.12454408299364772 1103 52-53 -0.18765979756913964 1103 54-55 -0.02255740695806452 1103 56-57 0.014784269162753105 1103 58-59 -0.15483222329350355 1103 60-61 -0.2211355320158468 1103 62-63 -0.3755239904459984 1103 64-65 -0.2965478422785601 1103 66-67 -0.3857526234495694 1103 68-69 -0.13773632128405922 1103 70-71 -0.33207274918291674 1103 72-73 -0.49958694083109023 1103 74-75 -0.27444105877539116 1103 76-77 0.12163841238284334 1104 1 0.028731164418779542 1104 2 0.018661920696345646 1104 3 0.2959557520686573 1104 4 0.45850271205176796 1104 5 0.14592379438416714 1104 6 0.34303034013967704 1104 7 0.15678636189137052 1104 8 0.292182281001395 1104 9 0.160629446726972 1104 10-11 0.3617287601136212 1104 12-13 0.4623777744863844 1104 14-15 0.5569297987585387 1104 16-17 0.5951773905792876 1104 18-19 0.49263551835883845 1104 20-21 0.6495631378508548 1104 22-23 0.4722077678502181 1104 24-25 0.27942937958216163 1104 26-27 0.4261113786369428 1104 28-29 0.15915041615383174 1104 30-31 0.2541499045179556 1104 32-33 0.11570664527363306 1104 34-35 0.4247390056193723 1104 36-37 0.6347870790309251 1104 38-39 0.4868293358302189 1104 40-41 0.4141267426935755 1104 42-43 0.5487335076238722 1104 44-45 0.24616077700978778 1104 46-47 0.20306484205818975 1104 48-49 0.3688409220748774 1104 50-51 0.3221159680033736 1104 52-53 0.22532820077546134 1104 54-55 0.08274855292961547 1104 56-57 0.19478462516291017 1104 58-59 0.24826675810103893 1104 60-61 0.3752225863453056 1104 62-63 0.021397275668572746 1104 64-65 0.19559456668552144 1104 66-67 -0.01927382103410835 1104 68-69 0.21384498692752985 1104 70-71 0.2603994049295544 1104 72-73 0.13840317126446067 1104 74-75 -0.14240393685868824 1104 76-77 -0.2347488979914445 1105 1 -0.07887414274286186 1105 2 0.1513787740435717 1105 3 0.161713803980696 1105 4 0.10452528177338394 1105 5 -0.13876055721949854 1105 6 -0.05428414868050169 1105 7 -0.18121748655705971 1105 8 -0.16395549009154564 1105 9 -0.12208446566621234 1105 10-11 0.25212360420104574 1105 12-13 0.3369187755871508 1105 14-15 0.24030038417644306 1105 16-17 0.33246616705837795 1105 18-19 0.29047853863460915 1105 20-21 0.20589711259226107 1105 22-23 0.33405435088175395 1105 24-25 0.48127965981306886 1105 26-27 0.38039424799976373 1105 28-29 0.4097112981039359 1105 30-31 0.5395064741891531 1105 32-33 0.6020017994216396 1105 34-35 0.7488678530174688 1105 36-37 0.7639369752572591 1105 38-39 0.7684740013597171 1105 40-41 0.6479388913545989 1105 42-43 0.7963912477947837 1105 44-45 0.44817911175757885 1105 46-47 0.711146163638908 1105 48-49 0.67354056663957 1105 50-51 0.6766122666026391 1105 52-53 0.8049062158835589 1105 54-55 0.2980747047332635 1105 56-57 0.41715436190551003 1105 58-59 0.48757008810128966 1105 60-61 0.8257956522478835 1105 62-63 0.3992867630182708 1105 64-65 0.3887333208973871 1105 66-67 0.5460153587758221 1105 68-69 0.20170304808256745 1105 70-71 0.011615699495511222 1105 72-73 0.03973416303819377 1105 74-75 0.17385365710479306 1105 76-77 0.24153440831311812 1106 1 0.3216202358859874 1106 2 0.22790413479292937 1106 3 0.392494039963033 1106 4 0.5204848889728737 1106 5 0.34931514152746956 1106 6 0.27370865024172275 1106 7 0.19630259260710403 1106 8 0.32425731526872426 1106 9 0.35558106573120085 1106 10-11 -0.02971294881947273 1106 12-13 0.5665087672017819 1106 14-15 0.4247534877535415 1106 16-17 0.42687599263491904 1106 18-19 0.3951912307328982 1106 20-21 0.2203002616728469 1106 22-23 0.3241657847784829 1106 24-25 0.05775534425325546 1106 26-27 -0.0024610667691966626 1106 28-29 0.09524337111618664 1106 30-31 0.02391208721467919 1106 32-33 0.08700750226192966 1106 34-35 0.07608570151664651 1106 36-37 -0.06842733887187435 1106 38-39 -0.18468322614585375 1106 40-41 -0.2358096034532302 1106 42-43 -0.09544653719819962 1106 44-45 -0.3291388841808853 1106 46-47 -0.26351891868466737 1106 48-49 -0.2321877079233765 1106 50-51 0.06245908870979022 1106 52-53 0.17498776451268938 1106 54-55 0.03863521246488233 1106 56-57 0.023264945343825616 1106 58-59 0.013420645866887071 1106 60-61 -0.04988143584609617 1106 62-63 0.2000907244058645 1106 64-65 0.11602826702840119 1106 66-67 0.14519411003263372 1106 68-69 0.1919545691711555 1106 70-71 -0.07276547278589973 1106 72-73 -0.021008040761845592 1106 74-75 0.012603364014569962 1106 76-77 0.09130728822760048 1107 1 0.18808355604659965 1107 2 0.4612778511992204 1107 3 0.4827194073774379 1107 4 0.28533220175626184 1107 5 0.4869996420809457 1107 6 0.33185467518465117 1107 7 0.2708065621573965 1107 8 -0.02380308387034802 1107 9 0.27617512624608054 1107 10-11 0.5044972385418021 1107 12-13 0.48590626917582824 1107 14-15 0.4517861905758096 1107 16-17 0.5612172227568379 1107 18-19 0.6818650033270472 1107 20-21 0.7096311035759229 1107 22-23 0.7289964465444179 1107 24-25 0.5572205329865625 1107 26-27 -0.09416486532922264 1107 28-29 0.032836589746661815 1107 30-31 0.3468410235842718 1107 32-33 0.41907769787405513 1107 34-35 0.4163488697273934 1107 36-37 0.5048194041928475 1107 38-39 0.5550802248708457 1107 40-41 0.6136349581788672 1107 42-43 0.7231759084479847 1107 44-45 0.6931271848909724 1107 46-47 0.7552676104182083 1107 48-49 0.6717936026162299 1107 50-51 0.7946297131042748 1107 52-53 0.7976088810642175 1107 54-55 0.9330729886206974 1107 56-57 1.070630743183301 1107 58-59 1.0122772884468247 1107 60-61 0.9741458703291563 1107 62-63 0.8081804681631333 1107 64-65 0.7397535401741528 1107 66-67 0.6721698783221512 1107 68-69 0.6658150851660238 1107 70-71 0.4503630144542292 1107 72-73 0.5250006618214904 1107 74-75 0.8261875701885124 1107 76-77 0.9997052251441367 1108 1 0.17893782846847017 1108 2 0.10289002854649709 1108 3 0.36878462499912956 1108 4 0.49474781484499886 1108 5 0.3289311603988949 1108 6 0.2831516446247093 1108 7 0.32918343395824223 1108 8 0.46813920867282377 1108 9 0.6833701891781487 1108 10-11 0.5158993714851476 1108 12-13 0.5806823595804076 1108 14-15 0.6677740810531603 1108 16-17 0.5979829416449292 1108 18-19 0.5247511886507894 1108 20-21 0.6956334421126726 1108 22-23 0.5297465798975303 1108 24-25 0.4716307942880178 1108 26-27 0.20425732886824122 1108 28-29 0.437966004088036 1108 30-31 0.4278958732220097 1108 32-33 0.22091673448169047 1108 34-35 0.3147779523639329 1108 36-37 0.3660641627972119 1108 38-39 0.3778265572186008 1108 40-41 0.5506154550649391 1108 42-43 0.5550662021074437 1108 44-45 0.3065859584662114 1108 46-47 0.5196052781693652 1108 48-49 0.31621855925439135 1108 50-51 0.34533392353019465 1108 52-53 0.44675506787161723 1108 54-55 0.19625021932688824 1108 56-57 0.3005200336034264 1108 58-59 0.5312964453725328 1108 60-61 0.5563249485500315 1108 62-63 0.41750529515733703 1108 64-65 0.4010463412254346 1108 66-67 0.5172868581478554 1108 68-69 0.5821187840811852 1108 70-71 0.6682010690177336 1108 72-73 0.4633968212517594 1108 74-75 0.3985921299819317 1108 76-77 0.6159449852145009 1109 1 -0.38496525412492844 1109 2 -0.5732142882011431 1109 3 -0.6348173183749708 1109 4 -0.47711374767244763 1109 5 -0.5832714698574293 1109 6 -0.5257184899595302 1109 7 -0.5567166330546556 1109 8 -0.20257266377928573 1109 9 -0.164786258021266 1109 10-11 -0.5441312729555747 1109 12-13 -0.5207444567484032 1109 14-15 -0.21613479536387814 1109 16-17 -0.6036316504140373 1109 18-19 -0.7666222965784542 1109 20-21 -0.7040374268212659 1109 22-23 -0.3432988771862888 1109 24-25 -0.29051703896154635 1109 26-27 -0.31696865817593434 1109 28-29 -0.3183757896239179 1109 30-31 -0.3363984669801461 1109 32-33 -0.3864225741685594 1109 34-35 -0.23035279259613972 1109 36-37 -0.16256465554198485 1109 38-39 -0.17589021555182072 1109 40-41 -0.14845125069258458 1109 42-43 0.0522705076862664 1109 44-45 -0.03653405046884117 1109 46-47 -0.06672792951478357 1109 48-49 -0.0660307008115062 1109 50-51 0.03834678163037708 1109 52-53 0.08056272688764565 1109 54-55 -0.19785020229594252 1109 56-57 -0.24928241795183226 1109 58-59 -0.1013541940422229 1109 60-61 0.010195990600625038 1109 62-63 -0.08958341316901297 1109 64-65 0.042150378752658924 1109 66-67 0.041067712003886925 1109 68-69 0.1961209899224734 1109 70-71 0.36000280856092814 1109 72-73 0.22559002781902393 1109 74-75 0.20021125937267215 1109 76-77 0.3709028927367015 1110 1 -0.23960099341930174 1110 2 -0.19204003557523208 1110 3 -0.7806023978255539 1110 4 -0.7161993927034231 1110 5 -0.6253297785027243 1110 6 -0.30247246248848825 1110 7 -0.5030302608488881 1110 8 -0.38166975508012513 1110 9 -0.7036194118052705 1110 10-11 -0.42485945771183253 1110 12-13 -0.25721092591080463 1110 14-15 -0.2496201845370578 1110 16-17 -0.6981946722292776 1110 18-19 -0.5810513297053106 1110 20-21 -0.6870918975996148 1110 22-23 -0.3033109967661396 1110 24-25 -0.05535847445845121 1110 26-27 -0.05081426819478807 1110 28-29 -0.1439918682665251 1110 30-31 0.18953911718360317 1110 32-33 0.08333907409430097 1110 34-35 0.09215427715877667 1110 36-37 0.14236902342381086 1110 38-39 -0.04406453550872769 1110 40-41 0.09068489258778101 1110 42-43 0.20074546055456466 1110 44-45 0.2876916430404499 1110 46-47 0.0852802175361198 1110 48-49 0.11452747317179401 1110 50-51 0.2206404121178558 1110 52-53 0.17682417315750953 1110 54-55 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-0.381413785929972 2314 4 -0.2570497733103849 2314 5 -0.4648240228079601 2314 6 -0.267277428298371 2314 7 -0.4982436444519678 2314 8 -0.7167515389871113 2314 9 -0.8456840074730891 2314 10-11 -0.7779501869576464 2314 12-13 -0.763142230296026 2314 14-15 -0.9043508925663559 2314 16-17 -1.0625136653052465 2314 18-19 -1.154959324601876 2314 20-21 -0.8795249150745619 2314 22-23 -0.6129881617818391 2314 24-25 -0.6778759279039832 2314 26-27 -0.5083819674937793 2314 28-29 -0.8414731246278961 2314 30-31 -1.0287665725679602 2314 32-33 -0.9225692935247203 2314 34-35 -0.9291589960611155 2314 36-37 -1.2335968294655046 2314 38-39 -0.9809167510569914 2314 40-41 -0.8343247838604633 2314 42-43 -0.7548561278164918 2314 44-45 -0.8768029596517515 2314 46-47 -0.7153741970746026 2314 48-49 -0.49520560021151994 2314 50-51 -0.7364470486387695 2314 52-53 -0.6223759071211745 2314 54-55 -0.5760617212954848 2314 56-57 -0.4457852130652 2314 58-59 -0.4874864559154304 2314 60-61 -0.614018636757752 2314 62-63 -0.8300580402864348 2314 64-65 -0.6565797723280404 2314 66-67 -0.7061409395948672 2314 68-69 -0.8539275028849289 2314 70-71 -0.5986646030677072 2314 72-73 -0.530485879990426 2314 74-75 -0.28571453157212545 2314 76-77 -0.11232567404482197 2315 1 -1.3667558714149095 2315 2 -1.363709439286957 2315 3 -0.9908888429126748 2315 4 -0.9780939123156855 2315 5 -0.9485171522474403 2315 6 -0.9037063127115701 2315 7 -1.5788008722514704 2315 8 -1.4263601298857438 2315 9 -1.2341168757998062 2315 10-11 -1.564170449006582 2315 12-13 -1.5113092270051425 2315 14-15 -1.4880533836825123 2315 16-17 -1.4590268308131868 2315 18-19 -1.502481665853196 2315 20-21 -1.506924332310188 2315 22-23 -1.3569020967675947 2315 24-25 -1.6413159800173176 2315 26-27 -1.5432912697498153 2315 28-29 -1.496095517498457 2315 30-31 -1.2805222120029818 2315 32-33 -1.1479250047325955 2315 34-35 -1.1209537465009198 2315 36-37 -0.9913816487266338 2315 38-39 -1.15506019014693 2315 40-41 -1.4712445061827317 2315 42-43 -1.6619076807496214 2315 44-45 -1.7783324235787425 2315 46-47 -1.4434187898611697 2315 48-49 -1.2717599310921806 2315 50-51 -1.0612536715049288 2315 52-53 -1.1127106260693402 2315 54-55 -1.3602224144756327 2315 56-57 -1.336826998649002 2315 58-59 -1.3040528529571418 2315 60-61 -1.3354547959738667 2315 62-63 -1.3021218869213662 2315 64-65 -1.30176832559793 2315 66-67 -1.3323421922392171 2315 68-69 -1.2310307920840238 2315 70-71 -1.0393310764330899 2315 72-73 -1.0834404641564497 2315 74-75 -1.4137634151271676 2315 76-77 -1.3681985008681075 2316 1 -2.0377725827301276 2316 2 -1.9009789322778445 2316 3 -1.7480035542562433 2316 4 -2.6990404616204415 2316 5 -2.4870112912390923 2316 6 -2.541666086492235 2316 7 -1.822736464943283 2316 8 -1.5967971187306915 2316 9 -1.9500797962403382 2316 10-11 -2.1167097468195095 2316 12-13 -2.6679469784190104 2316 14-15 -3.927710511150586 2316 16-17 -2.983192328113013 2316 18-19 -2.5763084011227164 2316 20-21 -2.199734498005924 2316 22-23 -2.2931609733249765 2316 24-25 -2.171024984850696 2316 26-27 -1.955721284916148 2316 28-29 -2.4863656766740903 2316 30-31 -2.7755648053042847 2316 32-33 -2.508401822920856 2316 34-35 -2.4932418784905437 2316 36-37 -2.4822832659964114 2316 38-39 -2.490696821803464 2316 40-41 -2.3061114818457256 2316 42-43 -2.304450663839866 2316 44-45 -2.3241227029511116 2316 46-47 -2.370853553365585 2316 48-49 -2.018266713419859 2316 50-51 -2.1457762154802076 2316 52-53 -2.291506816059556 2316 54-55 -2.3729536301919225 2316 56-57 -2.117298572404156 2316 58-59 -2.367449544712038 2316 60-61 -2.3173700062472875 2316 62-63 -2.20127569055278 2316 64-65 -2.3575081450193416 2316 66-67 -2.0765889073814563 2316 68-69 -2.2204767248135404 2316 70-71 -2.0188699915441397 2316 72-73 -1.7418039175447788 2316 74-75 -2.008475042177107 2316 76-77 -2.5747908950226908 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18054.0 3 3103.0 4 5560.0 5 7367.0 6 7653.0 7 7504.0 8 9980.0 9 11421.0 10 16674.0 11 19624.0 12 12246.0 13 7439.0 14 6455.0 15 6227.0 16 6153.0 17 6416.0 18 6588.0 19 6771.0 20 6905.0 21 7460.0 22 8134.0 23 8794.0 24 9820.0 25 11239.0 26 12236.0 27 13851.0 28 15576.0 29 17470.0 30 19634.0 31 22789.0 32 27315.0 33 34146.0 34 50866.0 35 61818.0 36 66438.0 37 80954.0 38 75014.0 39 23436.0 40 223.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.2868678398009 26.79290709155587 39.033854976803326 16.886370091839908 2 17.40214909480473 27.517827964203395 38.8112616463408 16.268761294651068 3 17.330615314691368 27.72541338412259 38.29849688338672 16.645474417799324 4 17.408878950337456 27.60139554577934 38.36235760116557 16.627367902717634 5 17.087410686023325 27.961328164102344 38.379781561212376 16.571479588661962 6 17.094464532344226 27.896208459643614 38.59920378869964 16.41012321931252 7 17.37764129758394 28.011417440004326 38.072292416330725 16.538648846081006 8 16.60673847227746 28.317221712674744 37.9100617138652 17.165978101182603 9 16.65731009657333 28.303331434964175 38.09012338497709 16.949235083485405 10-11 16.967522211587564 28.402558179647524 37.93384041477413 16.69607919399078 12-13 17.099395363529307 28.607645110385555 37.11198998382721 17.180969542257927 14-15 17.06516865179338 28.897881006019972 37.049829599906396 16.987120742280258 16-17 17.090104895814314 29.241517371044427 36.637923455136914 17.030454278004342 18-19 16.863057281760902 29.43972199151041 36.157539045908194 17.5396816808205 20-21 16.920813775751757 29.574377426380078 35.688990490619126 17.815818307249042 22-23 17.328378722045766 30.159122021957568 34.39155407416225 18.120945181834415 24-25 17.42174373016367 30.764204368660153 32.97505052937468 18.839001371801498 26-27 17.688364989590703 31.261236156446525 32.259284159606516 18.791114694356253 28-29 17.709674714049193 31.54190247914209 31.830626224150805 18.917796582657907 30-31 17.486601937545146 31.479427447564273 31.765108496576428 19.268862118314157 32-33 17.658290002651242 31.627159545284844 31.470857974558893 19.243692477505018 34-35 17.51721637784454 31.02823472439592 31.91769685377543 19.53685204398411 36-37 17.73066577751145 31.29706869603269 31.982534322315608 18.989731204140252 38-39 18.192812482244943 31.100434779079738 31.963499660455664 18.743253078219652 40-41 17.884197084636444 31.204278355535838 32.094903524624755 18.81662103520296 42-43 17.750082517626307 31.28939878469122 31.75731430828585 19.203204389396618 44-45 18.11166357666243 31.20825365947364 31.614159141193017 19.065923622670912 46-47 17.773406851472075 31.38023722045161 31.58403090792015 19.26232502015616 48-49 17.827914312923404 31.302688678224357 31.196567322468237 19.672829686384002 50-51 17.41261863369163 31.512558428995664 31.36049083738644 19.714332099926267 52-53 17.06963488312783 31.49842481493182 31.624656094176924 19.807284207763427 54-55 17.17962055910272 31.241540302489355 31.559533609815922 20.019305528592003 56-57 17.205864747193196 31.380334009486894 31.19223300445531 20.221568238864602 58-59 17.31507798116115 31.08228030470078 30.788523532332185 20.814118181805885 60-61 17.544320082684376 30.43317981078103 30.93805233114605 21.084447775388547 62-63 17.511641687659136 30.360655560275667 30.802038546771037 21.325664205294164 64-65 17.663380213096392 30.147349282193087 30.862247631210128 21.327022873500397 66-67 17.91635072548663 30.083800790997216 30.65347571373395 21.346372769782203 68-69 18.101969952491412 29.826978898826255 30.702197652779383 21.36885349590295 70-71 18.21490580515247 29.374095841785675 30.88157878711193 21.529419565949926 72-73 18.515646252979224 29.043995739422655 30.82909373405498 21.611264273543142 74-75 18.64284955300612 28.664392160737766 30.77472848395675 21.918029802299365 76-77 19.081016439618868 28.41040370495449 31.197173232881404 21.31140662254524 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 46944.0 1 27167.0 2 6594.0 3 5561.0 4 5324.0 5 5344.5 6 5171.5 7 5049.5 8 5011.0 9 4901.0 10 4879.0 11 4822.0 12 4945.0 13 5103.0 14 5172.0 15 5490.5 16 5963.0 17 6186.0 18 6476.5 19 7025.5 20 7671.0 21 8732.0 22 9406.0 23 9812.0 24 10753.5 25 12079.0 26 12869.0 27 13405.0 28 14399.5 29 15159.5 30 15461.0 31 16312.0 32 18183.5 33 19669.5 34 20543.5 35 20952.0 36 21235.0 37 21943.0 38 22389.5 39 22411.0 40 22689.0 41 22796.5 42 22875.5 43 23125.0 44 23030.0 45 23044.0 46 23026.5 47 22952.0 48 23004.0 49 23027.0 50 22987.0 51 22145.0 52 21366.0 53 20779.0 54 19215.0 55 17514.5 56 15983.0 57 15175.0 58 15159.0 59 13881.5 60 12011.5 61 11403.0 62 10396.5 63 7419.0 64 4479.5 65 3511.0 66 3104.0 67 2430.0 68 1923.5 69 1424.0 70 1164.0 71 989.0 72 807.5 73 767.0 74 733.0 75 712.5 76 499.0 77 242.5 78 179.0 79 150.0 80 118.0 81 84.0 82 56.5 83 60.0 84 47.5 85 29.0 86 24.0 87 25.0 88 21.0 89 14.5 90 14.0 91 16.0 92 10.0 93 3.5 94 4.0 95 5.5 96 6.0 97 5.5 98 5.0 99 11.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003110827980680406 2 0.008791470380183755 3 0.012037551751328526 4 0.019476488226868628 5 0.014472112779687104 6 0.013795845827365278 7 0.017988700931760606 8 0.018259207712689338 9 0.016500913636652587 10-11 0.02103190221720883 12-13 0.02008512848395827 14-15 0.008588590294487207 16-17 0.0064921627422895424 18-19 0.012849072094114717 20-21 0.011226031408542333 22-23 0.014742619560615837 24-25 0.024210356893121416 26-27 0.014877872951080201 28-29 0.008791470380183755 30-31 0.009061977161112485 32-33 0.01041451106575614 34-35 0.01163179157993543 36-37 0.010076377589595228 38-39 0.017177180588974415 40-41 0.01311957887504345 42-43 0.01582464668433076 44-45 0.019138354750707715 46-47 0.016906673808045683 48-49 0.014201605998758374 50-51 0.02813270521658802 52-53 0.03158166667342934 54-55 0.025224757321604157 56-57 0.01656854033188477 58-59 0.018394461103153705 60-61 0.021167155607673193 62-63 0.02691542470240873 64-65 0.02123478230290538 66-67 0.021572915779066292 68-69 0.030229132768785683 70-71 0.05491287652853238 72-73 0.06573314776568162 74-75 0.0790556067264216 76-77 0.07553901857434811 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 739353.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.56951888173617 #Duplication Level Percentage of deduplicated Percentage of total 1 83.63118338206144 49.81869357579649 2 8.71428094933529 10.38211047104365 3 3.1343231654374555 5.601303689549687 4 1.4981293304250702 3.56971373744156 5 0.8168277315484772 2.432901748880137 6 0.5095561453608968 1.8212408653428385 7 0.3620597338202634 1.509740690508579 8 0.23816642605561494 1.134996753113246 9 0.1660904794023361 0.8904536953950665 >10 0.849097959787755 8.785071258749545 >50 0.05276516532314251 2.1235501535525043 >100 0.025927492433319346 2.9671786985050805 >500 9.097365765078836E-4 0.41701182747117305 >1k 2.274341441269709E-4 0.18980947702635265 >5k 0.0 0.0 >10k+ 4.548682882539418E-4 8.35622335762411 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37392 5.057394776243553 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 24286 3.28476384081758 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 1401 0.18949000004057603 No Hit GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC 929 0.12565039974139552 No Hit AGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG 810 0.10955524627613604 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0012172805141792893 0.0 2 0.0 0.0 0.0 0.006897922913682639 0.0 3 0.0 0.0 0.0 0.010279257675291776 0.0 4 0.0 0.0 0.0 0.018935474665011165 0.0 5 0.0 0.0 0.0 0.036518415425378675 0.0 6 0.0 0.0 0.0 0.06181079944221502 0.0 7 0.0 0.0 0.0 0.10698543185731309 0.0 8 0.0 0.0 0.0 0.14986075663451692 0.0 9 0.0 0.0 0.0 0.19395336192590007 0.0 10 0.0 0.0 0.0 0.2537353605111496 0.0 11 0.0 0.0 0.0 0.3154109065629003 0.0 12 0.0 0.0 0.0 0.39439888659408967 0.0 13 0.0 0.0 0.0 0.4997612777658304 0.0 14 0.0 0.0 0.0 0.6336621343255522 0.0 15 0.0 0.0 0.0 0.7858221985979633 0.0 16 0.0 0.0 0.0 0.9451506925649859 0.0 17 0.0 0.0 0.0 1.082162377105388 0.0 18 0.0 0.0 0.0 1.2195798218171834 0.0 19 0.0 0.0 0.0 1.3487468097106525 0.0 20 0.0 0.0 0.0 1.4938736976789166 0.0 21 0.0 0.0 0.0 1.652120164522224 0.0 22 0.0 0.0 0.0 1.8146947398603914 0.0 23 0.0 0.0 0.0 1.9901183872926735 0.0 24 0.0 0.0 0.0 2.1614844330110246 0.0 25 0.0 0.0 0.0 2.3551672881559957 0.0 26 0.0 0.0 0.0 2.5664330840613347 0.0 27 0.0 0.0 0.0 2.7647145544820946 0.0 28 0.0 0.0 0.0 2.9404087086953052 0.0 29 0.0 0.0 0.0 3.123541799384056 0.0 30 0.0 0.0 0.0 3.3139785731578826 0.0 31 0.0 0.0 0.0 3.5141535910451434 0.0 32 0.0 0.0 0.0 3.7294769886644135 0.0 33 0.0 0.0 0.0 3.9519688159782946 0.0 34 0.0 0.0 0.0 4.185821928091182 0.0 35 0.0 0.0 0.0 4.43888102165001 0.0 36 0.0 0.0 0.0 4.717503006006603 0.0 37 0.0 0.0 0.0 4.991526375087408 0.0 38 0.0 0.0 0.0 5.253647445807347 0.0 39 0.0 0.0 0.0 5.486553784186985 0.0 40 0.0 0.0 0.0 5.727304819213556 0.0 41 0.0 0.0 0.0 5.995106532332999 0.0 42 0.0 0.0 0.0 6.262367231890585 0.0 43 0.0 0.0 0.0 6.5554613290268655 0.0 44 0.0 0.0 0.0 6.863839059285619 0.0 45 0.0 0.0 0.0 7.178573698896197 0.0 46 0.0 0.0 0.0 7.52008850981872 0.0 47 0.0 0.0 0.0 7.875128659787679 0.0 48 0.0 0.0 0.0 8.221918352938312 0.0 49 0.0 0.0 0.0 8.557211507899474 0.0 50 0.0 0.0 0.0 8.875192228881197 0.0 51 0.0 0.0 0.0 9.21021487706143 0.0 52 0.0 0.0 0.0 9.54266771082284 0.0 53 0.0 0.0 0.0 9.896220073496693 0.0 54 0.0 0.0 0.0 10.297922643175857 0.0 55 0.0 0.0 0.0 10.672168774590757 0.0 56 0.0 0.0 0.0 11.084962122288001 0.0 57 0.0 0.0 0.0 11.49518565556642 0.0 58 0.0 0.0 0.0 11.92894327878564 0.0 59 0.0 0.0 0.0 12.347282015491924 0.0 60 0.0 0.0 0.0 12.754529974180128 0.0 61 0.0 0.0 0.0 13.153121715878612 0.0 62 0.0 0.0 0.0 13.573354000051395 0.0 63 0.0 0.0 0.0 13.992910017271859 0.0 64 0.0 0.0 0.0 14.418958197234609 0.0 65 0.0 0.0 0.0 14.874221109537663 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACGG 25 2.2286622E-4 56.800053 55 GGCGATT 20 0.0065630525 53.268074 64 ATCGCGT 90 3.932655E-9 35.490425 2 TACGTAG 45 0.0040239063 31.547047 2 TAAGGCG 95 2.4925612E-7 29.894764 22 TCGCGAT 85 3.5558314E-6 29.221483 28 CGACGGC 50 0.006726368 28.400026 56 TGCGGAC 85 1.1904448E-4 25.052067 3 TGGCGAT 75 0.0016845887 23.673098 63 CGCGTCA 105 1.8302613E-5 23.661886 4 TTCGCGA 90 1.7552591E-4 23.658684 27 GCGTCAA 110 2.6225494E-5 22.586346 5 CGGTATA 80 0.0024593081 22.181519 1 CGGTTGC 100 3.577536E-4 21.295698 4 CGAATGT 110 6.787145E-4 19.366276 58 CGTCAAC 130 9.489542E-5 19.112816 6 GCAGTAC 150 1.3216659E-5 18.935913 49 AGGCGGA 170 1.8490337E-6 18.794136 24 CGGTTAC 95 0.006649495 18.680437 4 CGTGACC 95 0.006652092 18.679173 26 >>END_MODULE