FastQCFastQC Report
Thu 26 May 2016
SRR936180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936180_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739353
Sequences flagged as poor quality0
Sequence length125
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210672.8493831769127875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147491.9948522559589263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111691.510645118096498No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49860.6743734048553263No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47740.6456996860768808No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34850.4713580657683136No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29400.39764496796523446No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25620.34651918636970436No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20020.27077728770965964No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19060.2577929622250806No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG14460.19557640261147247No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13920.18827271952639674No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT11220.15175430410101806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10820.14634416848244344No Hit
GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG8280.11198980730449459No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7970.10779695220009929No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7820.1057681513431338No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC7710.10428036404802578No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA150.00423041559.52263698-99
GTATCGC300.004238653559.490436
CCCGGTA2450.055.8783998-99
CGTATCA10500.055.55822100-101
TACGCAG8250.055.19745100-101
AGGTATC15800.054.6289100-101
CGAGGTA1000.053.5703798-99
ACCGTAC950.053.25709598-99
CCGTACA4000.052.82991100-101
ACGTATC3500.052.72361100-101
CGGTATC10100.051.57013100-101
AGTATCA28700.051.43767100-101
TCCGGTA1800.051.255698-99
AAGTATC20550.051.12631100-101
CCGTAAA350.007798031450.9952472
TTACGCA4600.050.468258100-101
GTCGTAC950.050.12432598-99
CACGCAG5950.050.022404100-101
CCGGTAT4050.049.973100-101
CGAGTAC6350.049.68367100-101
Produced by FastQC (version 0.11.5)