FastQCFastQC Report
Thu 26 May 2016
SRR936179_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936179_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011295
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59570.19782186733614607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGT2600.021.8404122
ATTCGCG3150.020.28644656
TAAGGCG12800.019.96913717
CGCGGAA2605.820766E-1119.11448759
TCGCGCT3151.8189894E-1218.02850237
CTCGCGC3253.6379788E-1217.4737836
AACGTTC3950.017.0714594
CCTCGCG3409.094947E-1216.702635
CGATCGT3206.366463E-1116.6376321
TAACGTT3501.4551915E-1116.2243043
GGTAACG3301.0186341E-1016.1334611
CGGTTAC2452.1187589E-715.9345874
AATTCGC3201.1350494E-915.53129455
TCGCGGA3754.3655746E-1115.14570358
CGATTCG2352.4000856E-615.1029135
CGCGCTT4007.2759576E-1215.08403538
CAATTCG3301.7516868E-915.06164954
GTACATA17000.015.03733447
GGCACGC4101.0913936E-1114.71808642
CGGTCGG1707.3323125E-414.62157465
Produced by FastQC (version 0.11.5)