FastQCFastQC Report
Thu 26 May 2016
SRR936179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011295
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75400.2503906126766059No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54990.18261246407276605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36680.12180805932331439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71950.040.356151
CGTATCA12700.038.663788100-101
CGTACAT16250.033.879932100-101
GTATCAA139200.032.57141
CGGTATC12400.031.67936100-101
CCGTACA9000.027.77544100-101
CCGTATC5450.027.302202100-101
TACGCAG10850.027.153738100-101
TCGTACA9750.026.859766100-101
CCTGTCG8100.025.34870596-97
TATCAAC179550.025.0843222
AGTATCA29450.024.959496100-101
GCGTACA6350.024.838554100-101
ATCAACG182200.024.6864193
TCAACGC183100.024.5967544
GGGTATC13000.024.265356100-101
CAACGCA187600.024.0067445
CGAGTAC13750.023.80752100-101
TAAATCG3300.023.4450596-97
CCGGTAT5500.023.26644100-101
Produced by FastQC (version 0.11.5)