FastQCFastQC Report
Thu 26 May 2016
SRR936178_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936178_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023014
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59700.19748502653312225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA3100.025.19710259
TCGCGGA3350.023.31517858
CGCTAGT800.002458730222.18512220
ATTCGCG3600.021.69463256
TCGCGCT3450.021.60709437
AATTCGC3750.020.82650455
GATCGCG1901.1506927E-820.5558761
CTCGCGC3750.019.87655436
CAATTCG4200.018.5950954
TTACGCG1000.00893317517.75015356
CGCTCCG4450.016.75018125
TACGCGA1502.8356325E-416.56790757
TTGCGTA3004.5474735E-1016.56379536
ATCGCGG2401.6675949E-716.27366662
CGCGCTT4600.016.20612538
TCGAACT6400.016.08501224
ACGAGGT4000.015.9724954
CGTCGAC1350.002686368515.77399727
ATCGTTC4750.015.6914983
CGGTTAC3008.107236E-915.380924
Produced by FastQC (version 0.11.5)