FastQCFastQC Report
Thu 26 May 2016
SRR936178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023014
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74660.2469720616576701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54630.18071368508382693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36740.12153433626175732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14450.042.841393100-101
GGTATCA79750.037.0880471
CGGTATC11850.032.901905100-101
CGTACAT18450.031.617517100-101
GCGTACA6700.031.53927100-101
GTATCAA151050.030.0615981
CCGTACA8300.029.045242100-101
TACGCAG8850.028.921663100-101
CCTGTCG7900.028.62863796-97
TCGAGTA5000.028.571915100-101
AGTATCA33050.028.186485100-101
CGAGTAC14700.027.332823100-101
CGTCCTA4100.026.85876100-101
TCGTATC6850.026.503752100-101
TGTATCA23300.026.058073100-101
GGGTATC15300.024.704746100-101
GCGTATC5100.024.51022100-101
CCGTATC7650.024.121168100-101
AGGTATC25500.023.926641100-101
AGCGTAC3800.023.49508598-99
Produced by FastQC (version 0.11.5)